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The map label for this gene is gltD [H]

Identifier: 221229386

GI number: 221229386

Start: 82312

End: 83778

Strand: Direct

Name: gltD [H]

Synonym: MLBr_00062

Alternate gene names: 221229386

Gene position: 82312-83778 (Clockwise)

Preceding gene: 221229385

Following gene: 221229388

Centisome position: 2.52

GC content: 60.05

Gene sequence:

>1467_bases
ATGGCTGATCCGGCTGGCTTTTTGAAATACACACAGCGAGAATTACCGAGGCGACGGCCTGTCCCGCTACGGCTGCGTGA
CTGGAAGGAATTATACGAGGAATTCGATAAAGAGACTTTGCGGAATCAGGCGTCCCGTTGCATGGACTGCGGTATTCCTT
TCTGTCACAATGGGTGTCCTTTGGGAAATTTGATCCCGGAATGGAATGACTTGGTGCGTCGCGGCCGTTGGCACGATGCG
GTTGAACGTCTGCACGCCACAAACAATTTTCCCGATGTCACTGGCCGGCTATGTCCGGCGCCATGCGAGTCGGCGTGTGT
GCTCGGCATTAACCAGGATCCGGTGACGATCAAGCAGATCGAGCTAGAGATCATCGACAAGGCCTTCGACGAAGGTTGGG
TGCAACCACTGCCGCCGCAGCAGTTGACCGGTAAGACGGTTGCCGTGGTGGGTTCGGGGCCAGCTGGACTTGCTGCTGCC
CAACAACTCACCCGTGCGGGTCATCGCGTGACGGTCTTCGAACGCGACGATCGCATCGGCGGGTTGCTGCGGTATGGCAT
CCCGGAATTCAAGATGGAAAAGCGCTTCCTGGAGCGGAGGCTAGACCAGTTGCGGGCTGAAGGCACCGAATTCCGGTCTG
GCGTCAACGTCGGAGTTGACATCACTGCCGATCAGCTGTGCGCTGATTTTGATGCGGTCGTGCTGGCCGGCGGCGCGGCC
ACTTGGCGCGAACTGCCCATTCCGGGACGTGAGTTAGATGGCGTTCACCAGGCGATGGAGTTCCTGCCCTGGGTCAACCG
TGTCCAGGAGGGAGATGACGTCCTCGGAGACGACGGGCAGCCGCCTATCACTGCTAAGGACAAAAAGGTGGTCATCATCG
GTGGTGGCGACACCGGGGCAGACTGCTTGGGCGCGGTGCACCGGCAAGGTGCGATCCACGTGCACCAGCTCGAGATAATG
CCGCGTCCGCCGGATGCCCGCGCTGCATCCACGCCGTGGCCAACTTACCCACTGATGTATCGGGTATCGCCGGCCCACGA
AGAAGGTGGCGAGCGGGTGTTCTCAGTTAACACCGAGGAGTTCGTCGGCAAGAACGGGCGGGTGCACGCGCTCAAGGCTC
ATGAAGTGACGATGCAAGACGGCCGGTTTGTCAAGGTTGAGAACTCCGTTTTCGAGCTCGAGGCCGATCTAGTGTTGCTG
GCGATGGGATTCGTTGGTCCGGAAAAGCCGGGCCTGCTCACCGACCTGGGTGTGAAGTTCACCGACCGCGGCAACGTCGC
ACGTGGCGCCGACTTTGATACTTCGGTTCCTGGTGTTTTCGTTGCGGGCGACATGGGACGTGGCCAGTCGTTGATCGTTT
GGGCGATTGCTGAGGGTCGGGCTGCCGCGGCGGCTGTGGATCGATTTTTGATGGGCGCAACCGCGTTGCCGGCACCCGTC
AAGCCGTCGTCTTTACCACTTCAGTAG

Upstream 100 bases:

>100_bases
GTCAAGGTGATGCCGAAGGACTACAAGCGGGTGTTGCAGGCTATCGCGTTAGCTGAACGCAACGGTGTGGATGTCAACAA
GGTGATAATGGAGCCGGCAC

Downstream 100 bases:

>100_bases
TCGCACCAGTCACGCTAGAAACAGGCATATAATCGCTTCGGCGGGTCTTTCGTTGTTTGTCAGATAACGGGTGTCTAATC
ATTCTGTTGCAGGCTTCGTC

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MADPAGFLKYTQRELPRRRPVPLRLRDWKELYEEFDKETLRNQASRCMDCGIPFCHNGCPLGNLIPEWNDLVRRGRWHDA
VERLHATNNFPDVTGRLCPAPCESACVLGINQDPVTIKQIELEIIDKAFDEGWVQPLPPQQLTGKTVAVVGSGPAGLAAA
QQLTRAGHRVTVFERDDRIGGLLRYGIPEFKMEKRFLERRLDQLRAEGTEFRSGVNVGVDITADQLCADFDAVVLAGGAA
TWRELPIPGRELDGVHQAMEFLPWVNRVQEGDDVLGDDGQPPITAKDKKVVIIGGGDTGADCLGAVHRQGAIHVHQLEIM
PRPPDARAASTPWPTYPLMYRVSPAHEEGGERVFSVNTEEFVGKNGRVHALKAHEVTMQDGRFVKVENSVFELEADLVLL
AMGFVGPEKPGLLTDLGVKFTDRGNVARGADFDTSVPGVFVAGDMGRGQSLIVWAIAEGRAAAAAVDRFLMGATALPAPV
KPSSLPLQ

Sequences:

>Translated_488_residues
MADPAGFLKYTQRELPRRRPVPLRLRDWKELYEEFDKETLRNQASRCMDCGIPFCHNGCPLGNLIPEWNDLVRRGRWHDA
VERLHATNNFPDVTGRLCPAPCESACVLGINQDPVTIKQIELEIIDKAFDEGWVQPLPPQQLTGKTVAVVGSGPAGLAAA
QQLTRAGHRVTVFERDDRIGGLLRYGIPEFKMEKRFLERRLDQLRAEGTEFRSGVNVGVDITADQLCADFDAVVLAGGAA
TWRELPIPGRELDGVHQAMEFLPWVNRVQEGDDVLGDDGQPPITAKDKKVVIIGGGDTGADCLGAVHRQGAIHVHQLEIM
PRPPDARAASTPWPTYPLMYRVSPAHEEGGERVFSVNTEEFVGKNGRVHALKAHEVTMQDGRFVKVENSVFELEADLVLL
AMGFVGPEKPGLLTDLGVKFTDRGNVARGADFDTSVPGVFVAGDMGRGQSLIVWAIAEGRAAAAAVDRFLMGATALPAPV
KPSSLPLQ
>Mature_487_residues
ADPAGFLKYTQRELPRRRPVPLRLRDWKELYEEFDKETLRNQASRCMDCGIPFCHNGCPLGNLIPEWNDLVRRGRWHDAV
ERLHATNNFPDVTGRLCPAPCESACVLGINQDPVTIKQIELEIIDKAFDEGWVQPLPPQQLTGKTVAVVGSGPAGLAAAQ
QLTRAGHRVTVFERDDRIGGLLRYGIPEFKMEKRFLERRLDQLRAEGTEFRSGVNVGVDITADQLCADFDAVVLAGGAAT
WRELPIPGRELDGVHQAMEFLPWVNRVQEGDDVLGDDGQPPITAKDKKVVIIGGGDTGADCLGAVHRQGAIHVHQLEIMP
RPPDARAASTPWPTYPLMYRVSPAHEEGGERVFSVNTEEFVGKNGRVHALKAHEVTMQDGRFVKVENSVFELEADLVLLA
MGFVGPEKPGLLTDLGVKFTDRGNVARGADFDTSVPGVFVAGDMGRGQSLIVWAIAEGRAAAAAVDRFLMGATALPAPVK
PSSLPLQ

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=471, Percent_Identity=23.5668789808917, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI111118983, Length=157, Percent_Identity=33.1210191082803, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI111118981, Length=157, Percent_Identity=33.1210191082803, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1789606, Length=473, Percent_Identity=37.6321353065539, Blast_Score=265, Evalue=4e-72,
Organism=Escherichia coli, GI1788811, Length=474, Percent_Identity=34.8101265822785, Blast_Score=229, Evalue=4e-61,
Organism=Escherichia coli, GI87082180, Length=477, Percent_Identity=32.7044025157233, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI1788468, Length=452, Percent_Identity=25.8849557522124, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1789243, Length=431, Percent_Identity=28.0742459396752, Blast_Score=110, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17570289, Length=494, Percent_Identity=44.9392712550607, Blast_Score=426, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17543860, Length=171, Percent_Identity=35.672514619883, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6320030, Length=492, Percent_Identity=46.3414634146341, Blast_Score=415, Evalue=1e-116,
Organism=Drosophila melanogaster, GI28574881, Length=493, Percent_Identity=49.0872210953347, Blast_Score=457, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24665539, Length=493, Percent_Identity=49.0872210953347, Blast_Score=457, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24665547, Length=493, Percent_Identity=49.0872210953347, Blast_Score=456, Evalue=1e-128,
Organism=Drosophila melanogaster, GI24665543, Length=493, Percent_Identity=49.0872210953347, Blast_Score=456, Evalue=1e-128,
Organism=Drosophila melanogaster, GI24640763, Length=467, Percent_Identity=24.8394004282655, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI18858217, Length=467, Percent_Identity=24.8394004282655, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 53405; Mature: 53273

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADPAGFLKYTQRELPRRRPVPLRLRDWKELYEEFDKETLRNQASRCMDCGIPFCHNGCP
CCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
LGNLIPEWNDLVRRGRWHDAVERLHATNNFPDVTGRLCPAPCESACVLGINQDPVTIKQI
CCCCCCCHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEHH
ELEIIDKAFDEGWVQPLPPQQLTGKTVAVVGSGPAGLAAAQQLTRAGHRVTVFERDDRIG
HHHHHHHHHHCCCCCCCCCHHHCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCC
GLLRYGIPEFKMEKRFLERRLDQLRAEGTEFRSGVNVGVDITADQLCADFDAVVLAGGAA
HHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCEEECHHHHHHCCCEEEEECCCH
TWRELPIPGRELDGVHQAMEFLPWVNRVQEGDDVLGDDGQPPITAKDKKVVIIGGGDTGA
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCH
DCLGAVHRQGAIHVHQLEIMPRPPDARAASTPWPTYPLMYRVSPAHEEGGERVFSVNTEE
HHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEECHHH
FVGKNGRVHALKAHEVTMQDGRFVKVENSVFELEADLVLLAMGFVGPEKPGLLTDLGVKF
HHCCCCCEEEEEEEEEEECCCEEEEEECCEEEEHHCCCEEEHHCCCCCCCCCEEECCEEE
TDRGNVARGADFDTSVPGVFVAGDMGRGQSLIVWAIAEGRAAAAAVDRFLMGATALPAPV
ECCCCCCCCCCCCCCCCCEEEECCCCCCCEEEEEEEECCCHHHHHHHHHHHCCCCCCCCC
KPSSLPLQ
CCCCCCCC
>Mature Secondary Structure 
ADPAGFLKYTQRELPRRRPVPLRLRDWKELYEEFDKETLRNQASRCMDCGIPFCHNGCP
CCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
LGNLIPEWNDLVRRGRWHDAVERLHATNNFPDVTGRLCPAPCESACVLGINQDPVTIKQI
CCCCCCCHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEHH
ELEIIDKAFDEGWVQPLPPQQLTGKTVAVVGSGPAGLAAAQQLTRAGHRVTVFERDDRIG
HHHHHHHHHHCCCCCCCCCHHHCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCC
GLLRYGIPEFKMEKRFLERRLDQLRAEGTEFRSGVNVGVDITADQLCADFDAVVLAGGAA
HHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCEEECHHHHHHCCCEEEEECCCH
TWRELPIPGRELDGVHQAMEFLPWVNRVQEGDDVLGDDGQPPITAKDKKVVIIGGGDTGA
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCH
DCLGAVHRQGAIHVHQLEIMPRPPDARAASTPWPTYPLMYRVSPAHEEGGERVFSVNTEE
HHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEECHHH
FVGKNGRVHALKAHEVTMQDGRFVKVENSVFELEADLVLLAMGFVGPEKPGLLTDLGVKF
HHCCCCCEEEEEEEEEEECCCEEEEEECCEEEEHHCCCEEEHHCCCCCCCCCEEECCEEE
TDRGNVARGADFDTSVPGVFVAGDMGRGQSLIVWAIAEGRAAAAAVDRFLMGATALPAPV
ECCCCCCCCCCCCCCCCCEEEECCCCCCCEEEEEEEECCCHHHHHHHHHHHCCCCCCCCC
KPSSLPLQ
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]