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The map label for this gene is pbpA [H]

Identifier: 221229359

GI number: 221229359

Start: 22185

End: 23663

Strand: Reverse

Name: pbpA [H]

Synonym: MLBr_00018

Alternate gene names: 221229359

Gene position: 23663-22185 (Counterclockwise)

Preceding gene: 221229360

Following gene: 221229358

Centisome position: 0.72

GC content: 61.26

Gene sequence:

>1479_bases
ATGAACACCTCTCTCCGCCGAATCTCGGTGACCGTGATGGCGTTGATCGTGCTGCTGCTGCTCAACGCCACGGTAACGCA
GGTGTTCACCGCCGACGGACTGCGTGCCGACCCCCGGAACCAGCGGGTCTTACTCGATGAATATTCACGTCAACGTGGCC
AGATCACCGCAAGTGGCCAGCTACTAGCCTATTCGGTGGCCACTGACAACCGCTTCCGTTTCCTCCGGGTATACCCCAAC
CCTGCGGTGTACGCGCCGATTACTGGCTTCTATTCACTGCGATATTCCAGCACAGGCCTTGAGCAAGCCGAGGACGCGCT
GTTGAATGGATCCGACGAGCGGCTGTTCGGGCGCCGGTTGGCCGACTTCTTCACCGGCCGCGATCCACGCGGCGGCAATG
TCGATACCACGATCAACCCACGCGTCCAGCAGACTGGATGGGACGCGATGCAGCAGGGTTGCGGAGGATCGCCGTGTAAG
GGAGCTGTAGTCGCCCTAGAACCGTCCACCGGCAAGATCCTGGCTATGGTCTCGACACCATCGTATGACCCCAACCTGCT
GGCGTCTCACAATCCGGAAGAACAAGCACAGGCCTGGCGGCGCTTGCACGACGACCCTAACTCGCCCCTAATCAACCGTG
CCATTTCCGAAACCTATCCTCCTGGTTCCACATTTAAGGTGATCACCACCACTGCGGCGCTGCAGGCCGGCGCCACTACG
AGTGACCAGTTGACCGCAGAGCCCACAATCCCACTACCCGGCAGCACGGCCACGTTAGAGAACTACGGCGGCGTTTCCTG
CGGCCCCGGTCCGACGGTATCATTGAGCCAGGCATTCGCCATGTCGTGTAACACCGCGTTCGTCCAGCTGGGCCTGCTCA
TTGGCGCCGACGCGTTGCGGAGCATGGCACATTCCTTCGGCCTGGACAGTAACCCCAACGTAATTCCGTTGCAGGTCGCC
GAATCGACTGTTGGGGTAATCCCAGACGCCGCCGCGCTGGGGATGTCAAGTATCGGCCAGAGGGATGTTGCGCTGACTCC
TCTGCAAAACGCTCAAATCGCCGCGACCATCGCCAATGCCGGGGTGACGATGCAGCCCTACCTGATCGACAACCTCAAAG
GGCCAGATCTGGCCAACATCCGCACCACTACCTCATATCAGCAGCACCGCGCAGTATCACCACAGATCGCCGCTACGCTA
ACAGAGCTGATGGTCGGCGCCGAAAAGGTCACACAACAGAAAGGGGCCATTCCCGGCGTGCAGATTGCTTCCAAGACTGG
TACCGCAGAGCATGGCACCGACCCGCGTCATACACCGCCGCACGCGTGGTACATCGCCTTCGCACCCGTACAAGCCCCGA
AGGTTGCCGTAGCGGTGCTGGTAGAGAAGGGCGCCGACAGCCTGTTTGCTACGGGAGGTGCGTTAGCCGCACCGATCGGA
CGGGCCGTGATCGAGGCAGCGCTGCAGGGGGGGGCATGA

Upstream 100 bases:

>100_bases
TCCTGGCACGCATCTCGCATAGCGCTCGACATCCGTTACGCAGCCGCCCACACAACACGTCCCCGATCGCGGTGGCAAGT
ACCGAAGTGATCGAAAGGGT

Downstream 100 bases:

>100_bases
GTCCGCGAATTGGTGTGATGCTGTCTGGTAGATACCGCCTGCATCGGCTCATCGCCACCGGTGGCATGGGCCAAGTATGG
GAAGCGGTGGACAGTCGGCT

Product: putative penicillin-binding protein

Products: NA

Alternate protein names: PBPA [H]

Number of amino acids: Translated: 492; Mature: 492

Protein sequence:

>492_residues
MNTSLRRISVTVMALIVLLLLNATVTQVFTADGLRADPRNQRVLLDEYSRQRGQITASGQLLAYSVATDNRFRFLRVYPN
PAVYAPITGFYSLRYSSTGLEQAEDALLNGSDERLFGRRLADFFTGRDPRGGNVDTTINPRVQQTGWDAMQQGCGGSPCK
GAVVALEPSTGKILAMVSTPSYDPNLLASHNPEEQAQAWRRLHDDPNSPLINRAISETYPPGSTFKVITTTAALQAGATT
SDQLTAEPTIPLPGSTATLENYGGVSCGPGPTVSLSQAFAMSCNTAFVQLGLLIGADALRSMAHSFGLDSNPNVIPLQVA
ESTVGVIPDAAALGMSSIGQRDVALTPLQNAQIAATIANAGVTMQPYLIDNLKGPDLANIRTTTSYQQHRAVSPQIAATL
TELMVGAEKVTQQKGAIPGVQIASKTGTAEHGTDPRHTPPHAWYIAFAPVQAPKVAVAVLVEKGADSLFATGGALAAPIG
RAVIEAALQGGA

Sequences:

>Translated_492_residues
MNTSLRRISVTVMALIVLLLLNATVTQVFTADGLRADPRNQRVLLDEYSRQRGQITASGQLLAYSVATDNRFRFLRVYPN
PAVYAPITGFYSLRYSSTGLEQAEDALLNGSDERLFGRRLADFFTGRDPRGGNVDTTINPRVQQTGWDAMQQGCGGSPCK
GAVVALEPSTGKILAMVSTPSYDPNLLASHNPEEQAQAWRRLHDDPNSPLINRAISETYPPGSTFKVITTTAALQAGATT
SDQLTAEPTIPLPGSTATLENYGGVSCGPGPTVSLSQAFAMSCNTAFVQLGLLIGADALRSMAHSFGLDSNPNVIPLQVA
ESTVGVIPDAAALGMSSIGQRDVALTPLQNAQIAATIANAGVTMQPYLIDNLKGPDLANIRTTTSYQQHRAVSPQIAATL
TELMVGAEKVTQQKGAIPGVQIASKTGTAEHGTDPRHTPPHAWYIAFAPVQAPKVAVAVLVEKGADSLFATGGALAAPIG
RAVIEAALQGGA
>Mature_492_residues
MNTSLRRISVTVMALIVLLLLNATVTQVFTADGLRADPRNQRVLLDEYSRQRGQITASGQLLAYSVATDNRFRFLRVYPN
PAVYAPITGFYSLRYSSTGLEQAEDALLNGSDERLFGRRLADFFTGRDPRGGNVDTTINPRVQQTGWDAMQQGCGGSPCK
GAVVALEPSTGKILAMVSTPSYDPNLLASHNPEEQAQAWRRLHDDPNSPLINRAISETYPPGSTFKVITTTAALQAGATT
SDQLTAEPTIPLPGSTATLENYGGVSCGPGPTVSLSQAFAMSCNTAFVQLGLLIGADALRSMAHSFGLDSNPNVIPLQVA
ESTVGVIPDAAALGMSSIGQRDVALTPLQNAQIAATIANAGVTMQPYLIDNLKGPDLANIRTTTSYQQHRAVSPQIAATL
TELMVGAEKVTQQKGAIPGVQIASKTGTAEHGTDPRHTPPHAWYIAFAPVQAPKVAVAVLVEKGADSLFATGGALAAPIG
RAVIEAALQGGA

Specific function: Cell wall formation. Plays an important role in cell division and cell shape maintenance by cross-linking adjacent peptidoglycan chains through transpeptidation [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell membrane; Single-pass type II membrane protein (Probable). Note=Localized to the division site (septum) along with newly synthesized peptidoglycan, between segregated nucleoids [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=371, Percent_Identity=28.0323450134771, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1786272, Length=431, Percent_Identity=25.9860788863109, Blast_Score=95, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 51756; Mature: 51756

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTSLRRISVTVMALIVLLLLNATVTQVFTADGLRADPRNQRVLLDEYSRQRGQITASGQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHCCCEEECCC
LLAYSVATDNRFRFLRVYPNPAVYAPITGFYSLRYSSTGLEQAEDALLNGSDERLFGRRL
EEEEEEECCCCEEEEEECCCCEEEECCCCEEEEEECCCCHHHHHHHHHCCCCHHHHHHHH
ADFFTGRDPRGGNVDTTINPRVQQTGWDAMQQGCGGSPCKGAVVALEPSTGKILAMVSTP
HHHHCCCCCCCCCCCCCCCCCHHHCCHHHHHHCCCCCCCCCEEEEEECCCCEEEEEEECC
SYDPNLLASHNPEEQAQAWRRLHDDPNSPLINRAISETYPPGSTFKVITTTAALQAGATT
CCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCEEEEEEEHHHHCCCCC
SDQLTAEPTIPLPGSTATLENYGGVSCGPGPTVSLSQAFAMSCNTAFVQLGLLIGADALR
CCCEECCCCCCCCCCCCCHHCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHH
SMAHSFGLDSNPNVIPLQVAESTVGVIPDAAALGMSSIGQRDVALTPLQNAQIAATIANA
HHHHHCCCCCCCCEEEEEECCCCCCCCCCHHHHCHHHCCCCCEEECCCCCCEEEEEEECC
GVTMQPYLIDNLKGPDLANIRTTTSYQQHRAVSPQIAATLTELMVGAEKVTQQKGAIPGV
CCEECCCEECCCCCCCCCCEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCE
QIASKTGTAEHGTDPRHTPPHAWYIAFAPVQAPKVAVAVLVEKGADSLFATGGALAAPIG
EEECCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEEEECCCCCEEECCCHHHHHHH
RAVIEAALQGGA
HHHHHHHHCCCC
>Mature Secondary Structure
MNTSLRRISVTVMALIVLLLLNATVTQVFTADGLRADPRNQRVLLDEYSRQRGQITASGQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHCCCEEECCC
LLAYSVATDNRFRFLRVYPNPAVYAPITGFYSLRYSSTGLEQAEDALLNGSDERLFGRRL
EEEEEEECCCCEEEEEECCCCEEEECCCCEEEEEECCCCHHHHHHHHHCCCCHHHHHHHH
ADFFTGRDPRGGNVDTTINPRVQQTGWDAMQQGCGGSPCKGAVVALEPSTGKILAMVSTP
HHHHCCCCCCCCCCCCCCCCCHHHCCHHHHHHCCCCCCCCCEEEEEECCCCEEEEEEECC
SYDPNLLASHNPEEQAQAWRRLHDDPNSPLINRAISETYPPGSTFKVITTTAALQAGATT
CCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCEEEEEEEHHHHCCCCC
SDQLTAEPTIPLPGSTATLENYGGVSCGPGPTVSLSQAFAMSCNTAFVQLGLLIGADALR
CCCEECCCCCCCCCCCCCHHCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHH
SMAHSFGLDSNPNVIPLQVAESTVGVIPDAAALGMSSIGQRDVALTPLQNAQIAATIANA
HHHHHCCCCCCCCEEEEEECCCCCCCCCCHHHHCHHHCCCCCEEECCCCCCEEEEEEECC
GVTMQPYLIDNLKGPDLANIRTTTSYQQHRAVSPQIAATLTELMVGAEKVTQQKGAIPGV
CCEECCCEECCCCCCCCCCEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCE
QIASKTGTAEHGTDPRHTPPHAWYIAFAPVQAPKVAVAVLVEKGADSLFATGGALAAPIG
EEECCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEEEECCCCCEEECCCHHHHHHH
RAVIEAALQGGA
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]