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The map label for this gene is ykuT [H]

Identifier: 220933171

GI number: 220933171

Start: 2556807

End: 2557706

Strand: Reverse

Name: ykuT [H]

Synonym: Hore_23390

Alternate gene names: 220933171

Gene position: 2557706-2556807 (Counterclockwise)

Preceding gene: 220933172

Following gene: 220933170

Centisome position: 99.21

GC content: 38.44

Gene sequence:

>900_bases
ATGCCCCTGAACTGGGAAGAGGTAATAGAAATAATTTCCGGATTTTTTACCTTTGAGTTTTTAAGCAAATATATTATAAA
AATCATCAGTATTGTTATTACAGTAGCTATAGCTAAAATTACTATAAAGATAGGAAATAAATTTATCGATAATATGCTTA
AAAAGAAAAAGGATGACCATGTCAGTGAGAGGCGGAGACAGACCTTAAATACCCTTTTGAAAAGTATTTTAAGATATGGG
GTCTATTTTTTTATGGCCCTTATTATACTTTCTATCTTTAATGTCCCGATCGCATCATTACTGGCCGGAGCCGGTATTGT
GAGTGTGGCAATTGGTTTCGGAGCTCAGGACCTTGTTAAAGATATAATTAACGGTTTTTTTATTCTGTTTGAAGATCAGT
ACGGGGTTGGAGATTATATTGAGGCCGCCGGTGTTACCGGAGTAGTTGAAGAGATCGGATTAAGGAGTACTTTTATAAGA
AGTTTTGGGGGGGAACTCCACATTATTCCCAACAGTGAAATAAAAAAAGTAACCAATTATTCTTATGGAGATATGCGCGT
TCTGGTTGATGTCAGTGTCAGTTATGATGAATCCCCCTCCCGGGTAATTGAAGTTCTGGAGAATCTCTGTTACAGGATAG
CCGAGGAAAAGAAGGATGTTATAACCGAAGGTCCTACTGTTCTGGGGGTTCAGGAACTGGCTGATTCCGGTATGGTAATT
CGTATCTGGGCCAGAACTATCCCCATGGAACAATGGTATATCGGGCGTTATATTAAAAAACGTGTCAAAGAAACCCTTGA
TGAAGCAGGGATTGAAATACCTTATCCCCATATGGTATTATTAACAAAGGATCAGAAGACTGGTGACTTAAATACAGGTA
ACGAAGGGGTGGGAAAATGA

Upstream 100 bases:

>100_bases
TCACTAAAAAATCCCGGTTGTCTAATGCTTATTGCGATTATAATTGGGGCATTTATTTTAATCTACTTATGGTTACAATA
GTGATGGGAGGTAGGAAATT

Downstream 100 bases:

>100_bases
AAGATTACCACTTAGGTGATATAGTCCGTTTTAAAAAACAGCACCCCTGTGGTGGGGATACCTGGGAAGTAATCAGAATA
GGAATGGATTTTAAAGCCAG

Product: mechanosensitive ion channel MscS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MPLNWEEVIEIISGFFTFEFLSKYIIKIISIVITVAIAKITIKIGNKFIDNMLKKKKDDHVSERRRQTLNTLLKSILRYG
VYFFMALIILSIFNVPIASLLAGAGIVSVAIGFGAQDLVKDIINGFFILFEDQYGVGDYIEAAGVTGVVEEIGLRSTFIR
SFGGELHIIPNSEIKKVTNYSYGDMRVLVDVSVSYDESPSRVIEVLENLCYRIAEEKKDVITEGPTVLGVQELADSGMVI
RIWARTIPMEQWYIGRYIKKRVKETLDEAGIEIPYPHMVLLTKDQKTGDLNTGNEGVGK

Sequences:

>Translated_299_residues
MPLNWEEVIEIISGFFTFEFLSKYIIKIISIVITVAIAKITIKIGNKFIDNMLKKKKDDHVSERRRQTLNTLLKSILRYG
VYFFMALIILSIFNVPIASLLAGAGIVSVAIGFGAQDLVKDIINGFFILFEDQYGVGDYIEAAGVTGVVEEIGLRSTFIR
SFGGELHIIPNSEIKKVTNYSYGDMRVLVDVSVSYDESPSRVIEVLENLCYRIAEEKKDVITEGPTVLGVQELADSGMVI
RIWARTIPMEQWYIGRYIKKRVKETLDEAGIEIPYPHMVLLTKDQKTGDLNTGNEGVGK
>Mature_298_residues
PLNWEEVIEIISGFFTFEFLSKYIIKIISIVITVAIAKITIKIGNKFIDNMLKKKKDDHVSERRRQTLNTLLKSILRYGV
YFFMALIILSIFNVPIASLLAGAGIVSVAIGFGAQDLVKDIINGFFILFEDQYGVGDYIEAAGVTGVVEEIGLRSTFIRS
FGGELHIIPNSEIKKVTNYSYGDMRVLVDVSVSYDESPSRVIEVLENLCYRIAEEKKDVITEGPTVLGVQELADSGMVIR
IWARTIPMEQWYIGRYIKKRVKETLDEAGIEIPYPHMVLLTKDQKTGDLNTGNEGVGK

Specific function: May play a role in resistance to osmotic downshock [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI87081787, Length=255, Percent_Identity=30.9803921568627, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI1789291, Length=264, Percent_Identity=23.8636363636364, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1786670, Length=145, Percent_Identity=28.9655172413793, Blast_Score=64, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 33585; Mature: 33453

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLNWEEVIEIISGFFTFEFLSKYIIKIISIVITVAIAKITIKIGNKFIDNMLKKKKDDH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
VSERRRQTLNTLLKSILRYGVYFFMALIILSIFNVPIASLLAGAGIVSVAIGFGAQDLVK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCHHHHHH
DIINGFFILFEDQYGVGDYIEAAGVTGVVEEIGLRSTFIRSFGGELHIIPNSEIKKVTNY
HHHCCEEEEEECCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHCC
SYGDMRVLVDVSVSYDESPSRVIEVLENLCYRIAEEKKDVITEGPTVLGVQELADSGMVI
CCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHCCCCEE
RIWARTIPMEQWYIGRYIKKRVKETLDEAGIEIPYPHMVLLTKDQKTGDLNTGNEGVGK
EEEHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PLNWEEVIEIISGFFTFEFLSKYIIKIISIVITVAIAKITIKIGNKFIDNMLKKKKDDH
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
VSERRRQTLNTLLKSILRYGVYFFMALIILSIFNVPIASLLAGAGIVSVAIGFGAQDLVK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCHHHHHH
DIINGFFILFEDQYGVGDYIEAAGVTGVVEEIGLRSTFIRSFGGELHIIPNSEIKKVTNY
HHHCCEEEEEECCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHCC
SYGDMRVLVDVSVSYDESPSRVIEVLENLCYRIAEEKKDVITEGPTVLGVQELADSGMVI
CCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHCCCCEE
RIWARTIPMEQWYIGRYIKKRVKETLDEAGIEIPYPHMVLLTKDQKTGDLNTGNEGVGK
EEEHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]