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The map label for this gene is mtnU [H]

Identifier: 220933128

GI number: 220933128

Start: 2507276

End: 2508097

Strand: Reverse

Name: mtnU [H]

Synonym: Hore_22960

Alternate gene names: 220933128

Gene position: 2508097-2507276 (Counterclockwise)

Preceding gene: 220933129

Following gene: 220933127

Centisome position: 97.28

GC content: 41.12

Gene sequence:

>822_bases
ATGGAAAAAGTTGTTATTGGTGCTGTCCAGATGGCAAGCAAACTGGCTGATAAAGAAGGTAACATTAAACAGGCCTTAAC
TTATATTGCGGAATATGGAGACCGGGCTGATATACTTATTTTCCCGGAATTATTTACAACAGGTTATGACCTTGATATTG
TAGGAGATGACTACTATTCTCTGGCAGAGAAAATTCCAGGAAGGACTACAGAAATATTCAGTGAATACGCCCGGATGTAT
AAAACGGCTATTATTGGGAATATGGTTGAAAGGGATAAAAATGTCGGGGAAATTCTATATAATACCACCTTTGTTATTGA
TAAAAAGGGTGACTATACAGGTAAATACCGCAAGGTACATGTTTACCCGGCAGAATTTACTTACTTTAAAAGGGGTACTG
AGTTTCCTGTATTTAACGTAAACGGGGTAAAGATTGGTCTGGCGACATGTTATGACCATGGATTTGGAGAGATGTTCCGC
ATTCTGGCTAGAAAAGGGGCTCAAATAATATTTATTCCCTCAGCGATACCTAAAGGTTATGAATATCTGCTAAAATTAAG
AACCAGGGCCCGGGCCCAGGATAATCAGCTGTTTACAGTGGCTGTTAACAGTGCTGGCAAAACCCCCAATTCCCATTTTT
GTGGTAATAGTATGGTTGTTAACCCCCGGGGTGAAATTATACAGGAAGCTGATGACGGGGAGGGTGTTTTCCTCGCCGAA
CTTGACCTGGAGTTGATTGAGAGGGAACGAAAGCAGGAACCCCTCATCAGGGACAGTGCCTTTGATTTATACATGAAAGA
GTATAAGCGGTTTTTAGAGTAA

Upstream 100 bases:

>100_bases
TGAATCAGGCCATTTGTCCTGACTTTCCGGGATTCTGTTCAAAAATCAATTGATACTATTCTATATTATAAAATTACCTG
ACTGTAAAGGGGTAGTGATT

Downstream 100 bases:

>100_bases
CCAATGAATAATATGAATGATTACCAGGCATCCTTCAGTGGTGCCTGGTTCATTTTATCCATTTTATTAAGCTGGAGGTG
TATTATATGAAAGTCTTAAT

Product: Nitrilase/cyanide hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MEKVVIGAVQMASKLADKEGNIKQALTYIAEYGDRADILIFPELFTTGYDLDIVGDDYYSLAEKIPGRTTEIFSEYARMY
KTAIIGNMVERDKNVGEILYNTTFVIDKKGDYTGKYRKVHVYPAEFTYFKRGTEFPVFNVNGVKIGLATCYDHGFGEMFR
ILARKGAQIIFIPSAIPKGYEYLLKLRTRARAQDNQLFTVAVNSAGKTPNSHFCGNSMVVNPRGEIIQEADDGEGVFLAE
LDLELIERERKQEPLIRDSAFDLYMKEYKRFLE

Sequences:

>Translated_273_residues
MEKVVIGAVQMASKLADKEGNIKQALTYIAEYGDRADILIFPELFTTGYDLDIVGDDYYSLAEKIPGRTTEIFSEYARMY
KTAIIGNMVERDKNVGEILYNTTFVIDKKGDYTGKYRKVHVYPAEFTYFKRGTEFPVFNVNGVKIGLATCYDHGFGEMFR
ILARKGAQIIFIPSAIPKGYEYLLKLRTRARAQDNQLFTVAVNSAGKTPNSHFCGNSMVVNPRGEIIQEADDGEGVFLAE
LDLELIERERKQEPLIRDSAFDLYMKEYKRFLE
>Mature_273_residues
MEKVVIGAVQMASKLADKEGNIKQALTYIAEYGDRADILIFPELFTTGYDLDIVGDDYYSLAEKIPGRTTEIFSEYARMY
KTAIIGNMVERDKNVGEILYNTTFVIDKKGDYTGKYRKVHVYPAEFTYFKRGTEFPVFNVNGVKIGLATCYDHGFGEMFR
ILARKGAQIIFIPSAIPKGYEYLLKLRTRARAQDNQLFTVAVNSAGKTPNSHFCGNSMVVNPRGEIIQEADDGEGVFLAE
LDLELIERERKQEPLIRDSAFDLYMKEYKRFLE

Specific function: May be involved in a regulatory step in the methylthioribose (MTR) recycling pathway [H]

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=270, Percent_Identity=32.2222222222222, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI297632350, Length=281, Percent_Identity=30.6049822064057, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI5031947, Length=281, Percent_Identity=30.6049822064057, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI297632348, Length=281, Percent_Identity=30.6049822064057, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI7706509, Length=266, Percent_Identity=27.0676691729323, Blast_Score=79, Evalue=4e-15,
Organism=Escherichia coli, GI1786412, Length=227, Percent_Identity=25.1101321585903, Blast_Score=66, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17556280, Length=280, Percent_Identity=28.2142857142857, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17533173, Length=234, Percent_Identity=29.9145299145299, Blast_Score=88, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6323383, Length=278, Percent_Identity=30.5755395683453, Blast_Score=128, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6322335, Length=311, Percent_Identity=26.0450160771704, Blast_Score=73, Evalue=6e-14,
Organism=Drosophila melanogaster, GI21355835, Length=273, Percent_Identity=33.3333333333333, Blast_Score=131, Evalue=4e-31,
Organism=Drosophila melanogaster, GI21358471, Length=230, Percent_Identity=31.304347826087, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI17933642, Length=285, Percent_Identity=27.3684210526316, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 31097; Mature: 31097

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKVVIGAVQMASKLADKEGNIKQALTYIAEYGDRADILIFPELFTTGYDLDIVGDDYYS
CCCEEHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEEHHHHHCCCCEEEECCHHHH
LAEKIPGRTTEIFSEYARMYKTAIIGNMVERDKNVGEILYNTTFVIDKKGDYTGKYRKVH
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEEEEEECCCCCCCCEEEEE
VYPAEFTYFKRGTEFPVFNVNGVKIGLATCYDHGFGEMFRILARKGAQIIFIPSAIPKGY
EEECCEEEEECCCCCCEEECCCEEEEEEHHHHCCHHHHHHHHHHCCCEEEEECCCCCHHH
EYLLKLRTRARAQDNQLFTVAVNSAGKTPNSHFCGNSMVVNPRGEIIQEADDGEGVFLAE
HHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCEEECCCHHHHCCCCCCCEEEEEE
LDLELIERERKQEPLIRDSAFDLYMKEYKRFLE
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEKVVIGAVQMASKLADKEGNIKQALTYIAEYGDRADILIFPELFTTGYDLDIVGDDYYS
CCCEEHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEEHHHHHCCCCEEEECCHHHH
LAEKIPGRTTEIFSEYARMYKTAIIGNMVERDKNVGEILYNTTFVIDKKGDYTGKYRKVH
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEEEEEECCCCCCCCEEEEE
VYPAEFTYFKRGTEFPVFNVNGVKIGLATCYDHGFGEMFRILARKGAQIIFIPSAIPKGY
EEECCEEEEECCCCCCEEECCCEEEEEEHHHHCCHHHHHHHHHHCCCEEEEECCCCCHHH
EYLLKLRTRARAQDNQLFTVAVNSAGKTPNSHFCGNSMVVNPRGEIIQEADDGEGVFLAE
HHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCEEECCCHHHHCCCCCCCEEEEEE
LDLELIERERKQEPLIRDSAFDLYMKEYKRFLE
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 12022921 [H]