Click here to switch to the map view.

The map label for this gene is nagB

Identifier: 220933124

GI number: 220933124

Start: 2502976

End: 2503701

Strand: Reverse

Name: nagB

Synonym: Hore_22920

Alternate gene names: 220933124

Gene position: 2503701-2502976 (Counterclockwise)

Preceding gene: 220933125

Following gene: 220933123

Centisome position: 97.11

GC content: 48.07

Gene sequence:

>726_bases
ATGAGGGTTCTTATAGAAGAAAATTACCGGGAGATGAGTAAAAAAGCGGCTTTACTGGTAGCAAGCCAGGTTATCTTAAA
ACCCGATAGTGTCCTGGGGCTGGCCACAGGGAGTACCCCCATCGGGATGTATCAGGAGCTTGCTGAGATGTATAAAGAGG
GGGAGATAGATTTTTCAGAGGTTACTACCTTTAACCTCGATGAATATTATAACCTTCCCCCGGAGGCCCCCCAGAGTTAC
CATTATTATATGAAGGAAAACTTTTTTAAACATGTCAATATACATCCTGCCAGGACCCATATACCAGATGGGATGGCCGG
TGATGTCGAGGCAGAGTGTCAGGATTATGAAGAAAAAATAAGGCGGTCCGGGGGGATTGACCTTCAAATACTGGGGATTG
GCCCCAACGGCCATATCGGGTTTAATGAACCCGATGAGCGTCTCAATGTAACCACCCACCTGGTGGATCTGACTGAGGAG
ACCATCCAGGCCAATAGCCGGTTCTTTGATTCCCCTGATGATGTTCCCCGCAAGGCTATCTCAGTAGGGATGGCAACCAT
ACTTAAAGCGAGACGGATTATTCTTCTTGCCAGTGGCAGGAATAAGGCCAGGGCCATAAAGGAGACTGTAAGCGGCTATG
TCAGCACTAAAGTACCGGCTTCATTGCTCCAGACCCACCCCGAGGTAACCCTGATTATAGATAAAGAGGCAGCCTCTTTG
CTGTAA

Upstream 100 bases:

>100_bases
ATGGTTTTACTTGATAAAGAACTTAAAGTAAACAAGGTTTTTGTAGATGGAGAAATCAAATGTTAAATTTACATTAAATT
TTAATCAGGGAGGTAGGATA

Downstream 100 bases:

>100_bases
ATTATTAAATTTGTATACATGGTAAATACTGACAATGATTACCTGAAACTTTTGTAAAAATTGAACAGGGCTGCAGGTGT
GTTAATGTTCACCTGTCACA

Product: glucosamine-6-phosphate isomerase

Products: NA

Alternate protein names: GlcN6P deaminase; GNPDA; Glucosamine-6-phosphate isomerase

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MRVLIEENYREMSKKAALLVASQVILKPDSVLGLATGSTPIGMYQELAEMYKEGEIDFSEVTTFNLDEYYNLPPEAPQSY
HYYMKENFFKHVNIHPARTHIPDGMAGDVEAECQDYEEKIRRSGGIDLQILGIGPNGHIGFNEPDERLNVTTHLVDLTEE
TIQANSRFFDSPDDVPRKAISVGMATILKARRIILLASGRNKARAIKETVSGYVSTKVPASLLQTHPEVTLIIDKEAASL
L

Sequences:

>Translated_241_residues
MRVLIEENYREMSKKAALLVASQVILKPDSVLGLATGSTPIGMYQELAEMYKEGEIDFSEVTTFNLDEYYNLPPEAPQSY
HYYMKENFFKHVNIHPARTHIPDGMAGDVEAECQDYEEKIRRSGGIDLQILGIGPNGHIGFNEPDERLNVTTHLVDLTEE
TIQANSRFFDSPDDVPRKAISVGMATILKARRIILLASGRNKARAIKETVSGYVSTKVPASLLQTHPEVTLIIDKEAASL
L
>Mature_241_residues
MRVLIEENYREMSKKAALLVASQVILKPDSVLGLATGSTPIGMYQELAEMYKEGEIDFSEVTTFNLDEYYNLPPEAPQSY
HYYMKENFFKHVNIHPARTHIPDGMAGDVEAECQDYEEKIRRSGGIDLQILGIGPNGHIGFNEPDERLNVTTHLVDLTEE
TIQANSRFFDSPDDVPRKAISVGMATILKARRIILLASGRNKARAIKETVSGYVSTKVPASLLQTHPEVTLIIDKEAASL
L

Specific function: Catalyzes the reversible isomerization-deamination of glucosamine 6-phosphate (GlcN6P) to form fructose 6-phosphate (Fru6P) and ammonium ion

COG id: COG0363

COG function: function code G; 6-phosphogluconolactonase/Glucosamine-6-phosphate isomerase/deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. NagB subfamily

Homologues:

Organism=Homo sapiens, GI13027378, Length=243, Percent_Identity=44.0329218106996, Blast_Score=215, Evalue=2e-56,
Organism=Homo sapiens, GI19923881, Length=243, Percent_Identity=43.6213991769547, Blast_Score=211, Evalue=4e-55,
Organism=Escherichia coli, GI1786893, Length=241, Percent_Identity=41.49377593361, Blast_Score=191, Evalue=4e-50,
Organism=Escherichia coli, GI1789530, Length=238, Percent_Identity=31.0924369747899, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI48994958, Length=240, Percent_Identity=28.75, Blast_Score=94, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17554876, Length=242, Percent_Identity=44.6280991735537, Blast_Score=219, Evalue=1e-57,
Organism=Drosophila melanogaster, GI24581960, Length=243, Percent_Identity=41.9753086419753, Blast_Score=208, Evalue=3e-54,
Organism=Drosophila melanogaster, GI19920764, Length=243, Percent_Identity=41.9753086419753, Blast_Score=208, Evalue=3e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NAGB_HALOH (B8D185)

Other databases:

- EMBL:   CP001098
- RefSeq:   YP_002510032.1
- ProteinModelPortal:   B8D185
- GeneID:   7313044
- GenomeReviews:   CP001098_GR
- KEGG:   hor:Hore_22920
- HOGENOM:   HBG725991
- OMA:   HLSITMG
- ProtClustDB:   CLSK976844
- HAMAP:   MF_01241
- InterPro:   IPR006148
- InterPro:   IPR004547
- InterPro:   IPR018321
- PANTHER:   PTHR11280
- TIGRFAMs:   TIGR00502

Pfam domain/function: PF01182 Glucosamine_iso

EC number: =3.5.99.6

Molecular weight: Translated: 26894; Mature: 26894

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS01161 GLC_GALNAC_ISOMERASE

Important sites: ACT_SITE 67-67 ACT_SITE 136-136 ACT_SITE 138-138 ACT_SITE 143-143

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLIEENYREMSKKAALLVASQVILKPDSVLGLATGSTPIGMYQELAEMYKEGEIDFSE
CEEEEECHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCHHH
VTTFNLDEYYNLPPEAPQSYHYYMKENFFKHVNIHPARTHIPDGMAGDVEAECQDYEEKI
EEEECCHHHCCCCCCCCCHHHHHHHHCCHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
RRSGGIDLQILGIGPNGHIGFNEPDERLNVTTHLVDLTEETIQANSRFFDSPDDVPRKAI
HHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
SVGMATILKARRIILLASGRNKARAIKETVSGYVSTKVPASLLQTHPEVTLIIDKEAASL
HHHHHHHHHHCEEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEECCCHHHC
L
C
>Mature Secondary Structure
MRVLIEENYREMSKKAALLVASQVILKPDSVLGLATGSTPIGMYQELAEMYKEGEIDFSE
CEEEEECHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCHHH
VTTFNLDEYYNLPPEAPQSYHYYMKENFFKHVNIHPARTHIPDGMAGDVEAECQDYEEKI
EEEECCHHHCCCCCCCCCHHHHHHHHCCHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
RRSGGIDLQILGIGPNGHIGFNEPDERLNVTTHLVDLTEETIQANSRFFDSPDDVPRKAI
HHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
SVGMATILKARRIILLASGRNKARAIKETVSGYVSTKVPASLLQTHPEVTLIIDKEAASL
HHHHHHHHHHCEEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEECCCHHHC
L
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA