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The map label for this gene is buk1 [H]

Identifier: 220933020

GI number: 220933020

Start: 2378543

End: 2379622

Strand: Reverse

Name: buk1 [H]

Synonym: Hore_21870

Alternate gene names: 220933020

Gene position: 2379622-2378543 (Counterclockwise)

Preceding gene: 220933021

Following gene: 220933019

Centisome position: 92.3

GC content: 42.87

Gene sequence:

>1080_bases
ATGAATACCAAAATTCTCGTTATAAATCCCGGTTCAACTTCAACAAAGATAGCAGTTTTTGATAAAATAGAGGAATTATG
GGCTACTACCATTGACCATAAACCGGACGAGTTATCCCGGTTTAAAAAAATTATTGACCAGCTGGAATGGAGACAGGAAT
TAATTCAAAAAGAATTGAAAGAGGCCGGGTTTTTAATTAATGATTTCGGGGCTATTGCTGCCCGGGGTGGGTTGCTTGAC
CCTATACCGGGGGGAACCTATATAGTAGATTCTTTAATGGTTGATCATTTATATGAAGGTAAGAATGGAGAACATGCTTC
CAATCTGGCCGGTATTATTGCCTATCGGCTTGGTCAGGAATATAATGTTCCGGCCTATACCGTAGATCCTGTAGCTGTAG
ACGAGTTTATAGATGAATCGAGATTTTCGGGAATGCCGGAACTAAAAAGAAGATGTCAATCCCATGCCCTTAACCTTAAA
GCTATAGCCCGGAGGGTCGCTGAAGATTTAGGAAAGGATTTAAATACCATAAACCTGATAGGGGTTCACCTGGGTGGTGG
TATTTCTGTTGCTGCTATCAAGGGCGGACGGATCATTGATGTAAATAATGCCAACCAGGAAGGTCCCTATTCACCGGAAA
GGGTTGGCAGCCTGCCTTCACTGGAGCTTGTAAACTATATATATAAGAATAAACCACCCTATCATGAATTAAAAAAGAAA
TTACTGGGTCAGGGAGGACTGTCAGCCTATACCGGGACTAATGATGGAAGAGAAATTGAGAAAAGGATTAAGTCAGGGGA
CCGGGAGGCAAAACTGGCCTATGATGGCATGATCTATCAGATTGCAAAGGAAATCGGAAAAATGGCAACTGTATTCAGGG
GAAAGGTTGCGGCGGTTTACCTTACCGGTGGCCTGGCTTATTCAGAGTATGTTGTTCAAGGGATTAAAGAACGGGTCAGC
TTTATCGCACCGGTTATGGTTTATCCCGGGGCTGAAGAAATGAAACATCTGGCTGCCGGTGTCACCAGGGTTTTACTGGG
AGAAGAAAGTCCACGGGATTATGATAAAACAAGATTATAA

Upstream 100 bases:

>100_bases
GGGACAAAATTCCAGTAAAAAGAGGGAGGAAATTAAAGAGTCGATAGATAATTATTAAAAAAAGGAGACAATTCATACTT
TTTTGAAAGGGGTTCTCATA

Downstream 100 bases:

>100_bases
TAATAAAAATTAACTGATTTCAAACTGCTGTCAAGGACAGCAGTTTTTTTATTGATATAAATCACAGACCAGTGTTTATT
TTTAGTTTATAATTGTTATG

Product: butyrate kinase

Products: NA

Alternate protein names: BK 1; Branched-chain carboxylic acid kinase 1 [H]

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MNTKILVINPGSTSTKIAVFDKIEELWATTIDHKPDELSRFKKIIDQLEWRQELIQKELKEAGFLINDFGAIAARGGLLD
PIPGGTYIVDSLMVDHLYEGKNGEHASNLAGIIAYRLGQEYNVPAYTVDPVAVDEFIDESRFSGMPELKRRCQSHALNLK
AIARRVAEDLGKDLNTINLIGVHLGGGISVAAIKGGRIIDVNNANQEGPYSPERVGSLPSLELVNYIYKNKPPYHELKKK
LLGQGGLSAYTGTNDGREIEKRIKSGDREAKLAYDGMIYQIAKEIGKMATVFRGKVAAVYLTGGLAYSEYVVQGIKERVS
FIAPVMVYPGAEEMKHLAAGVTRVLLGEESPRDYDKTRL

Sequences:

>Translated_359_residues
MNTKILVINPGSTSTKIAVFDKIEELWATTIDHKPDELSRFKKIIDQLEWRQELIQKELKEAGFLINDFGAIAARGGLLD
PIPGGTYIVDSLMVDHLYEGKNGEHASNLAGIIAYRLGQEYNVPAYTVDPVAVDEFIDESRFSGMPELKRRCQSHALNLK
AIARRVAEDLGKDLNTINLIGVHLGGGISVAAIKGGRIIDVNNANQEGPYSPERVGSLPSLELVNYIYKNKPPYHELKKK
LLGQGGLSAYTGTNDGREIEKRIKSGDREAKLAYDGMIYQIAKEIGKMATVFRGKVAAVYLTGGLAYSEYVVQGIKERVS
FIAPVMVYPGAEEMKHLAAGVTRVLLGEESPRDYDKTRL
>Mature_359_residues
MNTKILVINPGSTSTKIAVFDKIEELWATTIDHKPDELSRFKKIIDQLEWRQELIQKELKEAGFLINDFGAIAARGGLLD
PIPGGTYIVDSLMVDHLYEGKNGEHASNLAGIIAYRLGQEYNVPAYTVDPVAVDEFIDESRFSGMPELKRRCQSHALNLK
AIARRVAEDLGKDLNTINLIGVHLGGGISVAAIKGGRIIDVNNANQEGPYSPERVGSLPSLELVNYIYKNKPPYHELKKK
LLGQGGLSAYTGTNDGREIEKRIKSGDREAKLAYDGMIYQIAKEIGKMATVFRGKVAAVYLTGGLAYSEYVVQGIKERVS
FIAPVMVYPGAEEMKHLAAGVTRVLLGEESPRDYDKTRL

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG3426

COG function: function code C; Butyrate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

None

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR011245 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.7 [H]

Molecular weight: Translated: 39579; Mature: 39579

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: PS01075 ACETATE_KINASE_1 ; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTKILVINPGSTSTKIAVFDKIEELWATTIDHKPDELSRFKKIIDQLEWRQELIQKELK
CCCEEEEECCCCCCCEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EAGFLINDFGAIAARGGLLDPIPGGTYIVDSLMVDHLYEGKNGEHASNLAGIIAYRLGQE
HCCCEEEHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCC
YNVPAYTVDPVAVDEFIDESRFSGMPELKRRCQSHALNLKAIARRVAEDLGKDLNTINLI
CCCCCEEECHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEE
GVHLGGGISVAAIKGGRIIDVNNANQEGPYSPERVGSLPSLELVNYIYKNKPPYHELKKK
EEEECCCEEEEEECCCEEEEECCCCCCCCCCHHHHCCCCHHHHHHHHHCCCCCHHHHHHH
LLGQGGLSAYTGTNDGREIEKRIKSGDREAKLAYDGMIYQIAKEIGKMATVFRGKVAAVY
HHCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
LTGGLAYSEYVVQGIKERVSFIAPVMVYPGAEEMKHLAAGVTRVLLGEESPRDYDKTRL
EECCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MNTKILVINPGSTSTKIAVFDKIEELWATTIDHKPDELSRFKKIIDQLEWRQELIQKELK
CCCEEEEECCCCCCCEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EAGFLINDFGAIAARGGLLDPIPGGTYIVDSLMVDHLYEGKNGEHASNLAGIIAYRLGQE
HCCCEEEHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCC
YNVPAYTVDPVAVDEFIDESRFSGMPELKRRCQSHALNLKAIARRVAEDLGKDLNTINLI
CCCCCEEECHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEE
GVHLGGGISVAAIKGGRIIDVNNANQEGPYSPERVGSLPSLELVNYIYKNKPPYHELKKK
EEEECCCEEEEEECCCEEEEECCCCCCCCCCHHHHCCCCHHHHHHHHHCCCCCHHHHHHH
LLGQGGLSAYTGTNDGREIEKRIKSGDREAKLAYDGMIYQIAKEIGKMATVFRGKVAAVY
HHCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
LTGGLAYSEYVVQGIKERVSFIAPVMVYPGAEEMKHLAAGVTRVLLGEESPRDYDKTRL
EECCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]