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The map label for this gene is dacF [H]

Identifier: 220932948

GI number: 220932948

Start: 2303163

End: 2304329

Strand: Reverse

Name: dacF [H]

Synonym: Hore_21160

Alternate gene names: 220932948

Gene position: 2304329-2303163 (Counterclockwise)

Preceding gene: 220932949

Following gene: 220932947

Centisome position: 89.38

GC content: 41.73

Gene sequence:

>1167_bases
ATGAAAAAGATAGCGACAACAGTAGCCATTATTATAGTTTCACTAATTCTTATGGTTAATAATGCCCGGGCCGGCGGGTT
TGATATAGGAGTAGATGCTGCTATATTAATAGATGCGGAATCAGGACAGGTTCTTTATGAAAAAAATGCTGATAAAAAAT
TACCTCCTGCCAGTATTACAAAAATAATGACCCTTTTGTTGACTATGGAGGAAGTTGATAAAGGGAATATAAGCCTTGAC
AGTATGGTTACTGTAAGTCAGCTGGCAGAATCAATGGGGGGATCCCAGATTTATCTTGCCGCTGATACCAGACTGAAGCT
CAGGGATTTATTAAAGGCAGTCACCATAGCATCAGCCAACGATGCTTGTGTTGCCGTTGCTGAGGCTGTGGCCGGTACAG
AAGAAAACTTTGTCAGATGGATGAACAAACGGGCCCGCGAACTGGGAATGAAAAATACCCATTTTTCAAATACAACAGGC
CTTCCTACTGATGGGGAACATTATACTACAGCCAGGGATGTTGCTATAATGGCCCGGGAACTTATTAAACACCCTCAGGT
GTTAAAGTGGGCTTCTATCTGGGTTGACTATATTGATCTCCCCAATGGCCGGAAGGCAATGCTGACCAATACCAATCGTT
TAATCAATGATTATCCAGGCATGGATGGATTGAAAACAGGGCATACCGATGCCGCTGGCTATTGCCTGGCAGCAACAGCC
AGAAGGAATGGGATGAGGTTAATATCTGTTATCATGAAGGGTGAGACAGAACGGGAACGGGAGGAAGCAACAGCCAGGCT
ACTGGATTATGGTTTTAATTCATTTGAGAAATATATAGTTCTTAAAAAAGGCCATAAAGTTCATAACATAGAAGTACCCA
ATGGAACCAGGACTTCTACGGTAGCTGAAACGGCTAATGACCTGAAGGTTGTAGTTAAAAAAGGTGAAAAAGATTTATTG
ACCAAGAAGGTAGTTATTAATGAGCCCGTCGAAGCCCCTGTAAAAAAGGGAGATGTTCTGGGTAAAATTCAGGTTTTATA
TAAAGATCAGGTTCTAAGCCAGGTTAAACTCCTGGCGACTGAAGATATAGAGAAGGCAGGATTCCTTACCAGGTTGTGGC
GCTCAATTGCAAGGTGGTTTCAAGAATTAATAAGTAATATTTTTTAA

Upstream 100 bases:

>100_bases
AGTGAAGAAGGAACCCTCTTAACAGATAAAACCCTTGAAGAACTGGATAGTTACTGGGAAGAGTCCAAAACAATGGAATA
ATAAAGGAGGATGTTGCCCA

Downstream 100 bases:

>100_bases
CCAGACTTTTTAAACCGGGTTTTCCATAACAGATAGTTCGGACTGTAAAACTCATAAAGAACTGTATTTCAGAATGCCAG
GGTTATAACCCTGGCATTTT

Product: peptidase S11 D-alanyl-D-alanine carboxypeptidase 1

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MKKIATTVAIIIVSLILMVNNARAGGFDIGVDAAILIDAESGQVLYEKNADKKLPPASITKIMTLLLTMEEVDKGNISLD
SMVTVSQLAESMGGSQIYLAADTRLKLRDLLKAVTIASANDACVAVAEAVAGTEENFVRWMNKRARELGMKNTHFSNTTG
LPTDGEHYTTARDVAIMARELIKHPQVLKWASIWVDYIDLPNGRKAMLTNTNRLINDYPGMDGLKTGHTDAAGYCLAATA
RRNGMRLISVIMKGETEREREEATARLLDYGFNSFEKYIVLKKGHKVHNIEVPNGTRTSTVAETANDLKVVVKKGEKDLL
TKKVVINEPVEAPVKKGDVLGKIQVLYKDQVLSQVKLLATEDIEKAGFLTRLWRSIARWFQELISNIF

Sequences:

>Translated_388_residues
MKKIATTVAIIIVSLILMVNNARAGGFDIGVDAAILIDAESGQVLYEKNADKKLPPASITKIMTLLLTMEEVDKGNISLD
SMVTVSQLAESMGGSQIYLAADTRLKLRDLLKAVTIASANDACVAVAEAVAGTEENFVRWMNKRARELGMKNTHFSNTTG
LPTDGEHYTTARDVAIMARELIKHPQVLKWASIWVDYIDLPNGRKAMLTNTNRLINDYPGMDGLKTGHTDAAGYCLAATA
RRNGMRLISVIMKGETEREREEATARLLDYGFNSFEKYIVLKKGHKVHNIEVPNGTRTSTVAETANDLKVVVKKGEKDLL
TKKVVINEPVEAPVKKGDVLGKIQVLYKDQVLSQVKLLATEDIEKAGFLTRLWRSIARWFQELISNIF
>Mature_388_residues
MKKIATTVAIIIVSLILMVNNARAGGFDIGVDAAILIDAESGQVLYEKNADKKLPPASITKIMTLLLTMEEVDKGNISLD
SMVTVSQLAESMGGSQIYLAADTRLKLRDLLKAVTIASANDACVAVAEAVAGTEENFVRWMNKRARELGMKNTHFSNTTG
LPTDGEHYTTARDVAIMARELIKHPQVLKWASIWVDYIDLPNGRKAMLTNTNRLINDYPGMDGLKTGHTDAAGYCLAATA
RRNGMRLISVIMKGETEREREEATARLLDYGFNSFEKYIVLKKGHKVHNIEVPNGTRTSTVAETANDLKVVVKKGEKDLL
TKKVVINEPVEAPVKKGDVLGKIQVLYKDQVLSQVKLLATEDIEKAGFLTRLWRSIARWFQELISNIF

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=367, Percent_Identity=35.149863760218, Blast_Score=196, Evalue=3e-51,
Organism=Escherichia coli, GI1786851, Length=396, Percent_Identity=32.5757575757576, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI87082022, Length=360, Percent_Identity=30.5555555555556, Blast_Score=164, Evalue=9e-42,
Organism=Escherichia coli, GI87082054, Length=213, Percent_Identity=29.1079812206573, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 42861; Mature: 42861

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIATTVAIIIVSLILMVNNARAGGFDIGVDAAILIDAESGQVLYEKNADKKLPPASIT
CCHHHHHHHHHHHHHHHHHCCCCCCCEECCCCEEEEEECCCCCEEEECCCCCCCCHHHHH
KIMTLLLTMEEVDKGNISLDSMVTVSQLAESMGGSQIYLAADTRLKLRDLLKAVTIASAN
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCCC
DACVAVAEAVAGTEENFVRWMNKRARELGMKNTHFSNTTGLPTDGEHYTTARDVAIMARE
CHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
LIKHPQVLKWASIWVDYIDLPNGRKAMLTNTNRLINDYPGMDGLKTGHTDAAGYCLAATA
HHCCCCHHHHHHHHHHHEECCCCCEEEEECCCHHHHCCCCCCCCCCCCCCCCCHHEEHHH
RRNGMRLISVIMKGETEREREEATARLLDYGFNSFEKYIVLKKGHKVHNIEVPNGTRTST
HHCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHEEEEECCCEEEEEECCCCCCCHH
VAETANDLKVVVKKGEKDLLTKKVVINEPVEAPVKKGDVLGKIQVLYKDQVLSQVKLLAT
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
EDIEKAGFLTRLWRSIARWFQELISNIF
HHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKIATTVAIIIVSLILMVNNARAGGFDIGVDAAILIDAESGQVLYEKNADKKLPPASIT
CCHHHHHHHHHHHHHHHHHCCCCCCCEECCCCEEEEEECCCCCEEEECCCCCCCCHHHHH
KIMTLLLTMEEVDKGNISLDSMVTVSQLAESMGGSQIYLAADTRLKLRDLLKAVTIASAN
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCCC
DACVAVAEAVAGTEENFVRWMNKRARELGMKNTHFSNTTGLPTDGEHYTTARDVAIMARE
CHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
LIKHPQVLKWASIWVDYIDLPNGRKAMLTNTNRLINDYPGMDGLKTGHTDAAGYCLAATA
HHCCCCHHHHHHHHHHHEECCCCCEEEEECCCHHHHCCCCCCCCCCCCCCCCCHHEEHHH
RRNGMRLISVIMKGETEREREEATARLLDYGFNSFEKYIVLKKGHKVHNIEVPNGTRTST
HHCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHEEEEECCCEEEEEECCCCCCCHH
VAETANDLKVVVKKGEKDLLTKKVVINEPVEAPVKKGDVLGKIQVLYKDQVLSQVKLLAT
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
EDIEKAGFLTRLWRSIARWFQELISNIF
HHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]