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The map label for this gene is rlmH

Identifier: 220932856

GI number: 220932856

Start: 2184448

End: 2184927

Strand: Reverse

Name: rlmH

Synonym: Hore_20240

Alternate gene names: 220932856

Gene position: 2184927-2184448 (Counterclockwise)

Preceding gene: 220932857

Following gene: 220932855

Centisome position: 84.75

GC content: 36.88

Gene sequence:

>480_bases
ATGAAGATTAATATTATGGCGGTAGGAAAAATAAAGGAGGACTACATTAATGCTGGAATAAAAGAATTTTTAAAAAGACT
AAAGCCCTATACCGAAGTAAATGTAATCGAGGTAGATGATGAAAGAATCCCTCCCAATGCCAGTGGAGCCCAGATAGAAA
AAGTCAAAGAAAAAGAAGGGGAGAGGCTATTAAAGAATGTCCCCAAAAACTCCTATGTTATCGTACTGGATGTTAAAGGG
AAGCCAATGACTTCAGAGGGACTGGCTAAATCTATTCAAAATCTGCAATTGCAAGGTTACAGTAATATAACTTTTATTAT
TGGAGGGGCCCTGGGGTTAAGCCAGAAGGTCCTGGATACCGGAGATTATATTTTATCATTTTCCCATATGACTTTTACCC
ACCAGATGATAAGGCTGATATTGCTGGAGCAGTTATATCGTGCTTTTAAAATAATTAAAGGTGAACCGTATCATAAATAA

Upstream 100 bases:

>100_bases
GCAGGGATTTGGCATAGGTTTTGCTATTCCTATCAGTACTGTCAGGGGTTTTTTAGAAAATTATTTATAAAAAAATTATT
TATAAAGGGAGGACAGGGCT

Downstream 100 bases:

>100_bases
TGCATAAAAATTTAATGAAATAAGGCCTCCCTAGAGGAGGTTTATTTTTAATAGAAAAATATTCATAAAGCTTTAAAAAA
TCTATTTAGTGAAAGTAATG

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MKINIMAVGKIKEDYINAGIKEFLKRLKPYTEVNVIEVDDERIPPNASGAQIEKVKEKEGERLLKNVPKNSYVIVLDVKG
KPMTSEGLAKSIQNLQLQGYSNITFIIGGALGLSQKVLDTGDYILSFSHMTFTHQMIRLILLEQLYRAFKIIKGEPYHK

Sequences:

>Translated_159_residues
MKINIMAVGKIKEDYINAGIKEFLKRLKPYTEVNVIEVDDERIPPNASGAQIEKVKEKEGERLLKNVPKNSYVIVLDVKG
KPMTSEGLAKSIQNLQLQGYSNITFIIGGALGLSQKVLDTGDYILSFSHMTFTHQMIRLILLEQLYRAFKIIKGEPYHK
>Mature_159_residues
MKINIMAVGKIKEDYINAGIKEFLKRLKPYTEVNVIEVDDERIPPNASGAQIEKVKEKEGERLLKNVPKNSYVIVLDVKG
KPMTSEGLAKSIQNLQLQGYSNITFIIGGALGLSQKVLDTGDYILSFSHMTFTHQMIRLILLEQLYRAFKIIKGEPYHK

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=161, Percent_Identity=32.9192546583851, Blast_Score=100, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_HALOH (B8CZR7)

Other databases:

- EMBL:   CP001098
- RefSeq:   YP_002509764.1
- ProteinModelPortal:   B8CZR7
- SMR:   B8CZR7
- GeneID:   7314348
- GenomeReviews:   CP001098_GR
- KEGG:   hor:Hore_20240
- HOGENOM:   HBG622807
- OMA:   TANHPYH
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR016051
- InterPro:   IPR003742
- PIRSF:   PIRSF004505
- TIGRFAMs:   TIGR00246

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 18008; Mature: 18008

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKINIMAVGKIKEDYINAGIKEFLKRLKPYTEVNVIEVDDERIPPNASGAQIEKVKEKEG
CEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHH
ERLLKNVPKNSYVIVLDVKGKPMTSEGLAKSIQNLQLQGYSNITFIIGGALGLSQKVLDT
HHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHCEECCCCCEEEEEECCCCCHHHHHCC
GDYILSFSHMTFTHQMIRLILLEQLYRAFKIIKGEPYHK
CCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKINIMAVGKIKEDYINAGIKEFLKRLKPYTEVNVIEVDDERIPPNASGAQIEKVKEKEG
CEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHH
ERLLKNVPKNSYVIVLDVKGKPMTSEGLAKSIQNLQLQGYSNITFIIGGALGLSQKVLDT
HHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHCEECCCCCEEEEEECCCCCHHHHHCC
GDYILSFSHMTFTHQMIRLILLEQLYRAFKIIKGEPYHK
CCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA