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The map label for this gene is xylF [H]

Identifier: 220932784

GI number: 220932784

Start: 2090139

End: 2091173

Strand: Reverse

Name: xylF [H]

Synonym: Hore_19500

Alternate gene names: 220932784

Gene position: 2091173-2090139 (Counterclockwise)

Preceding gene: 220932785

Following gene: 220932783

Centisome position: 81.11

GC content: 43.19

Gene sequence:

>1035_bases
ATGAAAAAAATATTTGCAATTTTAACACTGGTAATGCTTTTCACAGGACTGGTTGTAACAGAGGCACTGGCTGATGATGA
ATTGGTAATCGGTCTTTCAATGGACAACCTGAGGCTGGAGAGATGGCAGCATGACCGTGACATCTTTGTTAAAAGGGCTG
AAGAATTAGGTGCAAAAGTATTGGTACAGTCTGCCAATAGTGATGATATGGTGCAGTTATCCCAGGCTGAAAACCTGATT
ACCCAGGGCATCGACGTACTGGTAGTTGTGCCCCACAATGGTAAAATTATGGGCAGTATTGTCAGGGAAGCTCACCGTAA
TGGAGTTAAGGTCCTGGCTTATGATAGATTGTTAATGGACTGTGATGTTGACCATTATATTTCTTTCGATAATATCCGGG
TTGGAGAGTTACAGGCCCAATACCTGGTTGACAGGAAACCAAGTGGGAAGTACTTTCTTTTAGGTGGTTCTCCTACCGAC
AATAACGCTAAACTCTTCAGGCAGGGACAGATGAATGTACTTAACCCCTATATTGAAAGGGGAGATATTGAGGTAGTAGG
TGATCAGTGGGCCAAGGACTGGTTACCTCAGGAAGCCATGAAGATAATTGAGAATGCCCTGACTGCAAACAACAATGATA
TTGATGTAATTGTTGCTTCCAATGATAGTACTGCCGGTGGAGCTATTGAAGCCCTGGCAGAGCAGAACCTTGACGGAAAG
GTGCTGGTATCTGGTCAGGATGCCGACCTGGCTGCCTGTCAGCGTGTTGTTGAAGGTACTCAGACCATGACCATTTATAA
ACCAATTAGTAAGCTGGCTAACAGGGCAGCTGAAGTCGCAGTAGCCATGGCTAAAGGGGAAGAAGTTAAAACAAATGGTA
AGGTAAATAATGGCAAGATCGATGTTCCTTCCATTCTGCTGGAGCCAATTGCAGTCGATAAAGATAATATGGTTGAAACA
ATTATCAAAGATGGTTTCCATAGTTTAGAAGACGTATATAAAAATGTACCCAGGGAAGAATGGCCTGAACTATAA

Upstream 100 bases:

>100_bases
TTTAATACATATTTTACTACTAAGTGGGACGTTCTTTATGATTTAAATTTTCTAAATTATTACCTTAAAAACACTAATCA
AACTTAAGGGGGTAATTTTA

Downstream 100 bases:

>100_bases
TTTCAGTAAAAAAGAATAGAGGGGACAGGTACTGTCCCCTCTCCCTAACTATGTTAAAGGGGGGGTAATGGTGGAGGACT
ATATCCTGGAGACAAAAAAT

Product: D-xylose ABC transporter periplasmic substrate-binding protein

Products: ADP; phosphate; xylose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MKKIFAILTLVMLFTGLVVTEALADDELVIGLSMDNLRLERWQHDRDIFVKRAEELGAKVLVQSANSDDMVQLSQAENLI
TQGIDVLVVVPHNGKIMGSIVREAHRNGVKVLAYDRLLMDCDVDHYISFDNIRVGELQAQYLVDRKPSGKYFLLGGSPTD
NNAKLFRQGQMNVLNPYIERGDIEVVGDQWAKDWLPQEAMKIIENALTANNNDIDVIVASNDSTAGGAIEALAEQNLDGK
VLVSGQDADLAACQRVVEGTQTMTIYKPISKLANRAAEVAVAMAKGEEVKTNGKVNNGKIDVPSILLEPIAVDKDNMVET
IIKDGFHSLEDVYKNVPREEWPEL

Sequences:

>Translated_344_residues
MKKIFAILTLVMLFTGLVVTEALADDELVIGLSMDNLRLERWQHDRDIFVKRAEELGAKVLVQSANSDDMVQLSQAENLI
TQGIDVLVVVPHNGKIMGSIVREAHRNGVKVLAYDRLLMDCDVDHYISFDNIRVGELQAQYLVDRKPSGKYFLLGGSPTD
NNAKLFRQGQMNVLNPYIERGDIEVVGDQWAKDWLPQEAMKIIENALTANNNDIDVIVASNDSTAGGAIEALAEQNLDGK
VLVSGQDADLAACQRVVEGTQTMTIYKPISKLANRAAEVAVAMAKGEEVKTNGKVNNGKIDVPSILLEPIAVDKDNMVET
IIKDGFHSLEDVYKNVPREEWPEL
>Mature_344_residues
MKKIFAILTLVMLFTGLVVTEALADDELVIGLSMDNLRLERWQHDRDIFVKRAEELGAKVLVQSANSDDMVQLSQAENLI
TQGIDVLVVVPHNGKIMGSIVREAHRNGVKVLAYDRLLMDCDVDHYISFDNIRVGELQAQYLVDRKPSGKYFLLGGSPTD
NNAKLFRQGQMNVLNPYIERGDIEVVGDQWAKDWLPQEAMKIIENALTANNNDIDVIVASNDSTAGGAIEALAEQNLDGK
VLVSGQDADLAACQRVVEGTQTMTIYKPISKLANRAAEVAVAMAKGEEVKTNGKVNNGKIDVPSILLEPIAVDKDNMVET
IIKDGFHSLEDVYKNVPREEWPEL

Specific function: Involved in the high-affinity D-xylose membrane transport system. Binds with high affinity to xylose [H]

COG id: COG4213

COG function: function code G; ABC-type xylose transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1789990, Length=301, Percent_Identity=63.4551495016611, Blast_Score=412, Evalue=1e-116,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761
- InterPro:   IPR013456 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37998; Mature: 37998

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIFAILTLVMLFTGLVVTEALADDELVIGLSMDNLRLERWQHDRDIFVKRAEELGAKV
CHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHCCCHHHHHHHHHHCCCEE
LVQSANSDDMVQLSQAENLITQGIDVLVVVPHNGKIMGSIVREAHRNGVKVLAYDRLLMD
EEECCCCCCCEEHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEECHHHEE
CDVDHYISFDNIRVGELQAQYLVDRKPSGKYFLLGGSPTDNNAKLFRQGQMNVLNPYIER
CCHHHEEEECCEEECCCHHHHHHCCCCCCCEEEECCCCCCCCHHHEECCCCHHHCHHHHC
GDIEVVGDQWAKDWLPQEAMKIIENALTANNNDIDVIVASNDSTAGGAIEALAEQNLDGK
CCEEEECCHHHHHCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCE
VLVSGQDADLAACQRVVEGTQTMTIYKPISKLANRAAEVAVAMAKGEEVKTNGKVNNGKI
EEEECCCCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCC
DVPSILLEPIAVDKDNMVETIIKDGFHSLEDVYKNVPREEWPEL
CCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCC
>Mature Secondary Structure
MKKIFAILTLVMLFTGLVVTEALADDELVIGLSMDNLRLERWQHDRDIFVKRAEELGAKV
CHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHCCCHHHHHHHHHHCCCEE
LVQSANSDDMVQLSQAENLITQGIDVLVVVPHNGKIMGSIVREAHRNGVKVLAYDRLLMD
EEECCCCCCCEEHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEECHHHEE
CDVDHYISFDNIRVGELQAQYLVDRKPSGKYFLLGGSPTDNNAKLFRQGQMNVLNPYIER
CCHHHEEEECCEEECCCHHHHHHCCCCCCCEEEECCCCCCCCHHHEECCCCHHHCHHHHC
GDIEVVGDQWAKDWLPQEAMKIIENALTANNNDIDVIVASNDSTAGGAIEALAEQNLDGK
CCEEEECCHHHHHCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCE
VLVSGQDADLAACQRVVEGTQTMTIYKPISKLANRAAEVAVAMAKGEEVKTNGKVNNGKI
EEEECCCCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCC
DVPSILLEPIAVDKDNMVETIIKDGFHSLEDVYKNVPREEWPEL
CCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; xylose [Periplasm]; H2O [C]

Specific reaction: ATP + xylose [Periplasm] + H2O = ADP + phosphate + xylose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]