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The map label for this gene is bioB [H]

Identifier: 220932750

GI number: 220932750

Start: 2049371

End: 2050423

Strand: Reverse

Name: bioB [H]

Synonym: Hore_19160

Alternate gene names: 220932750

Gene position: 2050423-2049371 (Counterclockwise)

Preceding gene: 220932751

Following gene: 220932749

Centisome position: 79.53

GC content: 44.73

Gene sequence:

>1053_bases
ATGAACCTGGATGAGATACTACTTAAGGCAGAAAGGGAACATGCTTTAACCAGAGGAGAAATTGTTAAATTATTGAAAAC
CAGGGGTGAAGACCTGAAAAAACTATACAAAGCCGCGGACAGGGTCAGGTCTAAATACATGGGGGATACAGTTTATCTAA
GGGGAATTATTGAATTCTCCAATTATTGCAAACAAAACTGTAAATACTGTGGCTTAAGAAGGGATAACCACCGGCTGGAG
CGCTATCGGTTACAGCCTGAGGAGATTGTATCCATGGCTGAGAAAGCCACTAAACTGGGGTATAAAACAGTGGTACTCCA
GTCGGGTGAGGATGACTATTATGATACCGATATGGTGGAATGGATTATTAGAGAAATTAAAAACAGGATGGATACTGCTA
TAACCCTCTGTATAGGGGAACGGGAATATGAAGAATATAAAGTGTGGAGAGAGGCGGGAGCAGATAGATACCTCATCCGA
CATGAGACTGCTGACCCCGGACTATATCACCGGCTTCATCCCGGTATGTCCCTTGAGCAAAGGATTGAGAGACTACGATG
GTTAAAAGAACTTGGTTATCAGGTGGGAAGCGGTAATCTAATCGGGTTACCGGACCAGGATGAAGAGAGTATTTCCCGGG
ATATATTATTGTTTAAAGAGCTGGATCTTCATATGGTAGGACTGGGACCTCTGGTTGTTAATCCTGATACCCCCTTAAAG
GATTACCATAACGGTTCTGTAGAGATGGCCTTAAAAACTATTGCCATAACCAGGTTATTACTGCCCCTTGCCCATATACC
GGGAACCACAGCCCTGGGGAGTCTCGATCCTGAAGGAAGGCAGAAGGCCTTAAAGGCCGGGGCCAATGTTGTTATGCCCA
ACGTTACCCGGGGGAAGTATCGACCTCTTTATGAGCTTTATCCTGCTAAAATCTGTGTTGATGAAGAGCCGGAAAATTGT
CGCCAGTGTATTGGTAGTATCATAACATCACTGGGGAGGGTGGTTGGTACTGACTATGGTCATAGCCCCAGAATAGAAAA
GGGTGAGGTATAA

Upstream 100 bases:

>100_bases
TATGACAATCCAGAAGCCGGTAGAATTTACAGGAGGCTCGGTTTCAGGGAGATAGGGCAATGGAAAATAGTACAATTTGA
TGCCGGAGGAGATGGTTGAG

Downstream 100 bases:

>100_bases
TGAAAACTCCAAGAGTCAATATTAATTTGCCCAGGATCAGGGAAAATGCCTCTATATTTAACAGGAAATGTCAACAACAA
GGCATTTCTTTGACCGGTGT

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MNLDEILLKAEREHALTRGEIVKLLKTRGEDLKKLYKAADRVRSKYMGDTVYLRGIIEFSNYCKQNCKYCGLRRDNHRLE
RYRLQPEEIVSMAEKATKLGYKTVVLQSGEDDYYDTDMVEWIIREIKNRMDTAITLCIGEREYEEYKVWREAGADRYLIR
HETADPGLYHRLHPGMSLEQRIERLRWLKELGYQVGSGNLIGLPDQDEESISRDILLFKELDLHMVGLGPLVVNPDTPLK
DYHNGSVEMALKTIAITRLLLPLAHIPGTTALGSLDPEGRQKALKAGANVVMPNVTRGKYRPLYELYPAKICVDEEPENC
RQCIGSIITSLGRVVGTDYGHSPRIEKGEV

Sequences:

>Translated_350_residues
MNLDEILLKAEREHALTRGEIVKLLKTRGEDLKKLYKAADRVRSKYMGDTVYLRGIIEFSNYCKQNCKYCGLRRDNHRLE
RYRLQPEEIVSMAEKATKLGYKTVVLQSGEDDYYDTDMVEWIIREIKNRMDTAITLCIGEREYEEYKVWREAGADRYLIR
HETADPGLYHRLHPGMSLEQRIERLRWLKELGYQVGSGNLIGLPDQDEESISRDILLFKELDLHMVGLGPLVVNPDTPLK
DYHNGSVEMALKTIAITRLLLPLAHIPGTTALGSLDPEGRQKALKAGANVVMPNVTRGKYRPLYELYPAKICVDEEPENC
RQCIGSIITSLGRVVGTDYGHSPRIEKGEV
>Mature_350_residues
MNLDEILLKAEREHALTRGEIVKLLKTRGEDLKKLYKAADRVRSKYMGDTVYLRGIIEFSNYCKQNCKYCGLRRDNHRLE
RYRLQPEEIVSMAEKATKLGYKTVVLQSGEDDYYDTDMVEWIIREIKNRMDTAITLCIGEREYEEYKVWREAGADRYLIR
HETADPGLYHRLHPGMSLEQRIERLRWLKELGYQVGSGNLIGLPDQDEESISRDILLFKELDLHMVGLGPLVVNPDTPLK
DYHNGSVEMALKTIAITRLLLPLAHIPGTTALGSLDPEGRQKALKAGANVVMPNVTRGKYRPLYELYPAKICVDEEPENC
RQCIGSIITSLGRVVGTDYGHSPRIEKGEV

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 40067; Mature: 40067

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLDEILLKAEREHALTRGEIVKLLKTRGEDLKKLYKAADRVRSKYMGDTVYLRGIIEFS
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
NYCKQNCKYCGLRRDNHRLERYRLQPEEIVSMAEKATKLGYKTVVLQSGEDDYYDTDMVE
HHHHHCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHH
WIIREIKNRMDTAITLCIGEREYEEYKVWREAGADRYLIRHETADPGLYHRLHPGMSLEQ
HHHHHHHHHHHHEEEEEECCHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHCCCCCCHHH
RIERLRWLKELGYQVGSGNLIGLPDQDEESISRDILLFKELDLHMVGLGPLVVNPDTPLK
HHHHHHHHHHHCCCCCCCCEECCCCCCHHHHHHHHHHHHHCCEEEEECCCEEECCCCCCH
DYHNGSVEMALKTIAITRLLLPLAHIPGTTALGSLDPEGRQKALKAGANVVMPNVTRGKY
HCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCEECCCCCCCCC
RPLYELYPAKICVDEEPENCRQCIGSIITSLGRVVGTDYGHSPRIEKGEV
CCHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNLDEILLKAEREHALTRGEIVKLLKTRGEDLKKLYKAADRVRSKYMGDTVYLRGIIEFS
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
NYCKQNCKYCGLRRDNHRLERYRLQPEEIVSMAEKATKLGYKTVVLQSGEDDYYDTDMVE
HHHHHCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHH
WIIREIKNRMDTAITLCIGEREYEEYKVWREAGADRYLIRHETADPGLYHRLHPGMSLEQ
HHHHHHHHHHHHEEEEEECCHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHCCCCCCHHH
RIERLRWLKELGYQVGSGNLIGLPDQDEESISRDILLFKELDLHMVGLGPLVVNPDTPLK
HHHHHHHHHHHCCCCCCCCEECCCCCCHHHHHHHHHHHHHCCEEEEECCCEEECCCCCCH
DYHNGSVEMALKTIAITRLLLPLAHIPGTTALGSLDPEGRQKALKAGANVVMPNVTRGKY
HCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCEECCCCCCCCC
RPLYELYPAKICVDEEPENCRQCIGSIITSLGRVVGTDYGHSPRIEKGEV
CCHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA