Click here to switch to the map view.

The map label for this gene is nadE [H]

Identifier: 220932738

GI number: 220932738

Start: 2033830

End: 2034573

Strand: Reverse

Name: nadE [H]

Synonym: Hore_19030

Alternate gene names: 220932738

Gene position: 2034573-2033830 (Counterclockwise)

Preceding gene: 220932739

Following gene: 220932737

Centisome position: 78.92

GC content: 43.82

Gene sequence:

>744_bases
TTGTCAGAATTAGACTATAAACATGTAACTGCAGTATTAGTTGACTGGATAAGGGATAAAATTACGGAAGCAGGAGCAAA
TGGTGCTGTTGTCGGGATGAGTGGGGGTATTGATTCTGCTGTAACTTCAGTTTTATGTAAAAAGGCCTTCAAAGAAAATA
CCCTGGGAATTATAATGCCATGCCATAGCAGTGATAAAGACCTTGAAGATGCCAGGCTGGTTGCTGATGCCTTTGATATT
AAATACATAGTAAAGGACCTGGGGCCTGTTTTTGATAATTTCATCGAACTTCTGGGTGCCGATAGTCAGACCGGCGATGA
TATGGCGGTGGCCAACATAAAACCAAGGCTCAGGATGACCACCCTTTATTATTATGCTGCCCGCAATAATTATCTGGTGG
TCGGGACTGATAACTGGAGTGAGCTGACAGTTGGTTATTTTACCAAATACGGTGATGGTGGAGTGGATATTGCTCCACTG
GGAAGGCTTGTCAAGACTGAAGTCCGGGAACTGGCCAGATATCTGGGTATACCGGAAAGGATAATTCAACGTCCCCCGAC
AGCTGGACTCTGGGAAGGGCAATCAGATGAAAAGGAGATGGGTTTTACCTATGAAGAGCTGGACAGGTATATCCTGACCG
GTAAGGCTACTGGAGATACCAGGAGTAAAATTGAAACCCTTATTAACAAAAATAGACATAAAGTATCTCCCCTTCCTGTC
CCTGGAAGAGAGTCTATAATATGA

Upstream 100 bases:

>100_bases
TCTCAAACTTTCGTTATTTGTTGAAGGTGTTTTCATATAATGGTAAAATTAAAATATATCATTAGCAAAACATATTATAT
TTAGCAGGAGGAATGAAACC

Downstream 100 bases:

>100_bases
TTTGGAATAAACTCAACAAATTAACCATCTAGATAATTATTCTTAAGGCAAATTTGTCCAGAAGATGTTTTATTAAGTAA
AAGGGTACTGGTCCTTGAAA

Product: NH(3)-dependent NAD(+) synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MSELDYKHVTAVLVDWIRDKITEAGANGAVVGMSGGIDSAVTSVLCKKAFKENTLGIIMPCHSSDKDLEDARLVADAFDI
KYIVKDLGPVFDNFIELLGADSQTGDDMAVANIKPRLRMTTLYYYAARNNYLVVGTDNWSELTVGYFTKYGDGGVDIAPL
GRLVKTEVRELARYLGIPERIIQRPPTAGLWEGQSDEKEMGFTYEELDRYILTGKATGDTRSKIETLINKNRHKVSPLPV
PGRESII

Sequences:

>Translated_247_residues
MSELDYKHVTAVLVDWIRDKITEAGANGAVVGMSGGIDSAVTSVLCKKAFKENTLGIIMPCHSSDKDLEDARLVADAFDI
KYIVKDLGPVFDNFIELLGADSQTGDDMAVANIKPRLRMTTLYYYAARNNYLVVGTDNWSELTVGYFTKYGDGGVDIAPL
GRLVKTEVRELARYLGIPERIIQRPPTAGLWEGQSDEKEMGFTYEELDRYILTGKATGDTRSKIETLINKNRHKVSPLPV
PGRESII
>Mature_246_residues
SELDYKHVTAVLVDWIRDKITEAGANGAVVGMSGGIDSAVTSVLCKKAFKENTLGIIMPCHSSDKDLEDARLVADAFDIK
YIVKDLGPVFDNFIELLGADSQTGDDMAVANIKPRLRMTTLYYYAARNNYLVVGTDNWSELTVGYFTKYGDGGVDIAPLG
RLVKTEVRELARYLGIPERIIQRPPTAGLWEGQSDEKEMGFTYEELDRYILTGKATGDTRSKIETLINKNRHKVSPLPVP
GRESII

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788036, Length=223, Percent_Identity=32.2869955156951, Blast_Score=95, Evalue=5e-21,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729 [H]

Pfam domain/function: PF02540 NAD_synthase [H]

EC number: =6.3.1.5 [H]

Molecular weight: Translated: 27356; Mature: 27224

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELDYKHVTAVLVDWIRDKITEAGANGAVVGMSGGIDSAVTSVLCKKAFKENTLGIIMP
CCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEE
CHSSDKDLEDARLVADAFDIKYIVKDLGPVFDNFIELLGADSQTGDDMAVANIKPRLRMT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCEEEE
TLYYYAARNNYLVVGTDNWSELTVGYFTKYGDGGVDIAPLGRLVKTEVRELARYLGIPER
EEEEEEECCCEEEEECCCCCCEEEHHHEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHH
IIQRPPTAGLWEGQSDEKEMGFTYEELDRYILTGKATGDTRSKIETLINKNRHKVSPLPV
HHCCCCCCCCCCCCCCCHHHCCCHHHHHHEEEECCCCCCHHHHHHHHHCCCCCCCCCCCC
PGRESII
CCCCCCC
>Mature Secondary Structure 
SELDYKHVTAVLVDWIRDKITEAGANGAVVGMSGGIDSAVTSVLCKKAFKENTLGIIMP
CCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEE
CHSSDKDLEDARLVADAFDIKYIVKDLGPVFDNFIELLGADSQTGDDMAVANIKPRLRMT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCEEEE
TLYYYAARNNYLVVGTDNWSELTVGYFTKYGDGGVDIAPLGRLVKTEVRELARYLGIPER
EEEEEEECCCEEEEECCCCCCEEEHHHEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHH
IIQRPPTAGLWEGQSDEKEMGFTYEELDRYILTGKATGDTRSKIETLINKNRHKVSPLPV
HHCCCCCCCCCCCCCCCHHHCCCHHHHHHEEEECCCCCCHHHHHHHHHCCCCCCCCCCCC
PGRESII
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA