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The map label for this gene is ywlF [H]

Identifier: 220932629

GI number: 220932629

Start: 1917743

End: 1918711

Strand: Reverse

Name: ywlF [H]

Synonym: Hore_17930

Alternate gene names: 220932629

Gene position: 1918711-1917743 (Counterclockwise)

Preceding gene: 220932630

Following gene: 220932628

Centisome position: 74.42

GC content: 42.11

Gene sequence:

>969_bases
ATGCCTAAATTACTCTTTGTCTGTACAGGGAATACCTGCCGTAGTCCCATGGCCAGGTTTCTGCTGGAGAAGATGTTATC
TCAAAATAAAGATTTAAACAAAAAATGGGAAATAGATTCAGCCGGTATTGCGGCCTTTCCCGGTGGTAAAGCCAGTGATA
ATGCTGTCAGGGTTCTGGGCGAAGAAGGAATAGACCTGACAGGTCATCTTTCTAAACAGGTAGATGGTGACTTAATTAAA
GATGTCGATATTATTTTAACTATGACGATTAATCATAAAGATATGCTCCTTGATAACTTTCCTGAAGCTAAAGGCAAGGT
CTATACCCTTAAGGAATTTGCCGGTCAGGCTGAAAATCCCGATGTTTCAGATCCTTTCGGGCAGTCCCTGGAGATATACC
GCCAGACCCGGGATGAAATAAAGAAGTATCTTCAAAAAATAATGGGAAAATTGAATTATTATAATAGTGAAGAAGGAATA
GAAGGGATGGTCGAGAATAATATTAAAAGAGGTGATATAGATATGAAAATAGCTATTGGATCAGATCATGCCGGCTATGA
AATGAAACAGGAGATTATTAAACTACTGGACGAGCTGGGATATGATGTATCAGATATGGGTACTGATTCCGATGAATCTG
TAGACTATCCTGATTTTGCCTATAAAGTAGCATCAGGGGTTGCCTCCGGGGAGTATGACCGGGGACTTCTGGTGTGTGGT
ACCGGAATTGGCATGTCTATTGCTGCCAATAAGGTTGAGGGAGTCAGGGCTGCCCTCTGCCATGATGTTTTTTCTGCCCG
GGCTGCCAGAAATCATAATAATGCCAATGTCCTGGCCCTGGGTTCTAGGGTTATAGGGATAGGTCTGGCTAAAGAGATAG
TCAAAACCTGGCTGGAAGCTGGTTTTGATGGAGACAGGCATGAAAGAAGGGTTAATAAAATTACCAGGATTGAAAGGGGA
GAATACTAA

Upstream 100 bases:

>100_bases
GATGATACCCAGGTTATCGGGGGACTGGCTTTAATTTATCTGGGCCTCCATTTTATGGGGTTAGTCTAGAATAGTACAGG
GATGGTAAGGAGGGATCAGG

Downstream 100 bases:

>100_bases
AATGGGAAAGGTAACCGTTATCGACCATCCACTAATACAACATAAATTAACCCTGATCAGGAATAAAGATACCGGTCCCA
AAGAATTCAGGGAACTGGTA

Product: ribose-5-phosphate isomerase

Products: D-ribulose 5-phosphate

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MPKLLFVCTGNTCRSPMARFLLEKMLSQNKDLNKKWEIDSAGIAAFPGGKASDNAVRVLGEEGIDLTGHLSKQVDGDLIK
DVDIILTMTINHKDMLLDNFPEAKGKVYTLKEFAGQAENPDVSDPFGQSLEIYRQTRDEIKKYLQKIMGKLNYYNSEEGI
EGMVENNIKRGDIDMKIAIGSDHAGYEMKQEIIKLLDELGYDVSDMGTDSDESVDYPDFAYKVASGVASGEYDRGLLVCG
TGIGMSIAANKVEGVRAALCHDVFSARAARNHNNANVLALGSRVIGIGLAKEIVKTWLEAGFDGDRHERRVNKITRIERG
EY

Sequences:

>Translated_322_residues
MPKLLFVCTGNTCRSPMARFLLEKMLSQNKDLNKKWEIDSAGIAAFPGGKASDNAVRVLGEEGIDLTGHLSKQVDGDLIK
DVDIILTMTINHKDMLLDNFPEAKGKVYTLKEFAGQAENPDVSDPFGQSLEIYRQTRDEIKKYLQKIMGKLNYYNSEEGI
EGMVENNIKRGDIDMKIAIGSDHAGYEMKQEIIKLLDELGYDVSDMGTDSDESVDYPDFAYKVASGVASGEYDRGLLVCG
TGIGMSIAANKVEGVRAALCHDVFSARAARNHNNANVLALGSRVIGIGLAKEIVKTWLEAGFDGDRHERRVNKITRIERG
EY
>Mature_321_residues
PKLLFVCTGNTCRSPMARFLLEKMLSQNKDLNKKWEIDSAGIAAFPGGKASDNAVRVLGEEGIDLTGHLSKQVDGDLIKD
VDIILTMTINHKDMLLDNFPEAKGKVYTLKEFAGQAENPDVSDPFGQSLEIYRQTRDEIKKYLQKIMGKLNYYNSEEGIE
GMVENNIKRGDIDMKIAIGSDHAGYEMKQEIIKLLDELGYDVSDMGTDSDESVDYPDFAYKVASGVASGEYDRGLLVCGT
GIGMSIAANKVEGVRAALCHDVFSARAARNHNNANVLALGSRVIGIGLAKEIVKTWLEAGFDGDRHERRVNKITRIERGE
Y

Specific function: In Addition To Its Activity On D-Ribose 5-Phosphate It Probably Also Has Activity On D-Allose 6-Phosphate. [C]

COG id: COG0698

COG function: function code G; Ribose 5-phosphate isomerase RpiB

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lacAB/rpiB family [H]

Homologues:

Organism=Escherichia coli, GI1790528, Length=144, Percent_Identity=54.1666666666667, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI1788375, Length=130, Percent_Identity=34.6153846153846, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87081812, Length=149, Percent_Identity=28.1879194630872, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003500
- InterPro:   IPR004785 [H]

Pfam domain/function: PF02502 LacAB_rpiB [H]

EC number: 5.3.1.6

Molecular weight: Translated: 35564; Mature: 35433

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKLLFVCTGNTCRSPMARFLLEKMLSQNKDLNKKWEIDSAGIAAFPGGKASDNAVRVLG
CCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCEEECCCCCCCCCCEEEEC
EEGIDLTGHLSKQVDGDLIKDVDIILTMTINHKDMLLDNFPEAKGKVYTLKEFAGQAENP
CCCCCCHHHHHHHCCCHHHCCEEEEEEEEECCHHHHHHCCCCCCCCEEEHHHHCCCCCCC
DVSDPFGQSLEIYRQTRDEIKKYLQKIMGKLNYYNSEEGIEGMVENNIKRGDIDMKIAIG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCEEEEEEC
SDHAGYEMKQEIIKLLDELGYDVSDMGTDSDESVDYPDFAYKVASGVASGEYDRGLLVCG
CCCCCHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEE
TGIGMSIAANKVEGVRAALCHDVFSARAARNHNNANVLALGSRVIGIGLAKEIVKTWLEA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHC
GFDGDRHERRVNKITRIERGEY
CCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PKLLFVCTGNTCRSPMARFLLEKMLSQNKDLNKKWEIDSAGIAAFPGGKASDNAVRVLG
CCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCEEECCCCCCCCCCEEEEC
EEGIDLTGHLSKQVDGDLIKDVDIILTMTINHKDMLLDNFPEAKGKVYTLKEFAGQAENP
CCCCCCHHHHHHHCCCHHHCCEEEEEEEEECCHHHHHHCCCCCCCCEEEHHHHCCCCCCC
DVSDPFGQSLEIYRQTRDEIKKYLQKIMGKLNYYNSEEGIEGMVENNIKRGDIDMKIAIG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCEEEEEEC
SDHAGYEMKQEIIKLLDELGYDVSDMGTDSDESVDYPDFAYKVASGVASGEYDRGLLVCG
CCCCCHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEE
TGIGMSIAANKVEGVRAALCHDVFSARAARNHNNANVLALGSRVIGIGLAKEIVKTWLEA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHC
GFDGDRHERRVNKITRIERGEY
CCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 4.4 {D-ribose} 0.83 {D-ribose} [C]

Substrates: D-ribose 5-phosphate

Specific reaction: D-ribose 5-phosphate = D-ribulose 5-phosphate

General reaction: Intramolecular oxidoreduction; Isomerization [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]