Click here to switch to the map view.

The map label for this gene is gltB [C]

Identifier: 220932466

GI number: 220932466

Start: 1751909

End: 1753411

Strand: Reverse

Name: gltB [C]

Synonym: Hore_16300

Alternate gene names: 220932466

Gene position: 1753411-1751909 (Counterclockwise)

Preceding gene: 220932467

Following gene: 220932465

Centisome position: 68.01

GC content: 47.7

Gene sequence:

>1503_bases
ATGAGTTCAAGCCATCGCAATCCAGAGTTTATTATTGAAAGGGATTATGAAAAGTGTATAAACTGCCGCCTCTGTGAACG
ACAGTGTGCCTATGAAGTCCATTATTATGATGAAGACCAGGATAAAATGATGGCCCGTAACCGTAACTGTGTTAACTGTC
ACCGCTGTGTAAGTTTATGTCCCACTGATGCCCTTGGAATCAAAAAATATCCCCTTCAGTTCCGGGAGAATTATAACTGG
ACTTCCGAAATGATAAATAATATTTATAAACAGGCTGAAACAGGTGGGGTCCTTCTTAGCGGTATGGGGAGTCCGGCCCC
TAAGAAAATATACTGGGACCACATTCTATTAAACGCAAGCCAGGTTACAAATCCTTCCATAGATCCCTTAAGAGAACCGA
TGGAATTAAAAACTTTCCTGGGACGTAAACCTGATAAACTTACCATTACCGGGGAAGGTGAGCTGGAAAAGTTACCTCCC
CAGTTAGAACTTGAAACCCCGATTATGTTTTCAGCCATGTCTTTTGGTTCTATCAGTTTAAATGCCTGTAAATCTCTTGC
CAGGGCGGCTTCTGAACTGGGCATTATGTATAATACCGGTGAAGGGGGGCTTCACCGGGAACTGTATCAGTATGGTAATA
ATACTATAGTGCAGGTGGCTTCGGGGCGGTTTGGGGTCCATAAGGAGTATCTCGAGGCCGGAGCTGCTATAGAAATAAAG
ATAGGCCAGGGAGCCAAGCCGGGAATTGGTGGACACCTTCCCGGGGAAAAGGTGGGGGATGAGGTATCCAGAACCAGGAT
GATTCCCCCGGGTACAGATGCCCTGTCTCCTGCTCCCCATCATGATATCTATTCTATTGAAGACCTACGCCAGCTGATTT
ACGCCCTGAAGGAAGCGACTGATTATAAAAAGCCAGTTTCGGTCAAGATTTCAGCTGTGCATAATGTTGCGGCTATTGCA
TCCGGTCTTGCCACTGCTGGAGCTGATATTATTGCTATAGATGGTTTCCGGGGGGGAACCGGGGCCGCTCCTACCATGAT
CAGGGATAATGTCGGTATTCCCGTAGAGCTGGCCCTGGCAGCGGTCGATACCCGCTTGCGGCAGGAGGGTTTGCGTAATC
GGGTTTCCCTGGTGGTCGGGGGGAGTATAAGAAACAGTGCCGATGTAGTTAAAGCAATTGCTCTGGGGGCTGACGCTGTT
TATATCGGTTCAGCTGCCCTGATAGCCCTGGGATGCCATATGTGTCAGAAGTGTTATACAGGTAAATGCAACTGGGGGAT
AGCAACCCAGCGTCTGGACCTTGTCAACAGGTTGAACCCGGAAAAAGGGGTTGAAAGGGTTAAAAACCTGATCAGGGCCT
GGTCCCATGAAATAAAAGAGATGCTCGGTGGTATGGGTATCAATGCTATTGAAAGCCTCAGGGGAAACAGGCTCATGTTG
CGGGGAATGGGGTTGAATAGTGAAGAACTTAAAATTCTGGGAATCAAACATGCCGGACAGTAG

Upstream 100 bases:

>100_bases
AAGAATCTGCCATCAGGGAAATTTGTCCTCAACCAGATACAGTCTGGACACCCCGGGGAGGAGAACCGGTTATAGCACTA
ATGGAAGGAGGAGATGAGGA

Downstream 100 bases:

>100_bases
ATGAGAGGTTTAAGTACTAAATATTAGTTAACCAGGGTCTTTCAAAATTTTTTGTGTCAAAAACTGGACGTTGCCCCATT
TTAGACCCGTTAAAAAACCA

Product: Glutamate synthase (NADPH)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 500; Mature: 499

Protein sequence:

>500_residues
MSSSHRNPEFIIERDYEKCINCRLCERQCAYEVHYYDEDQDKMMARNRNCVNCHRCVSLCPTDALGIKKYPLQFRENYNW
TSEMINNIYKQAETGGVLLSGMGSPAPKKIYWDHILLNASQVTNPSIDPLREPMELKTFLGRKPDKLTITGEGELEKLPP
QLELETPIMFSAMSFGSISLNACKSLARAASELGIMYNTGEGGLHRELYQYGNNTIVQVASGRFGVHKEYLEAGAAIEIK
IGQGAKPGIGGHLPGEKVGDEVSRTRMIPPGTDALSPAPHHDIYSIEDLRQLIYALKEATDYKKPVSVKISAVHNVAAIA
SGLATAGADIIAIDGFRGGTGAAPTMIRDNVGIPVELALAAVDTRLRQEGLRNRVSLVVGGSIRNSADVVKAIALGADAV
YIGSAALIALGCHMCQKCYTGKCNWGIATQRLDLVNRLNPEKGVERVKNLIRAWSHEIKEMLGGMGINAIESLRGNRLML
RGMGLNSEELKILGIKHAGQ

Sequences:

>Translated_500_residues
MSSSHRNPEFIIERDYEKCINCRLCERQCAYEVHYYDEDQDKMMARNRNCVNCHRCVSLCPTDALGIKKYPLQFRENYNW
TSEMINNIYKQAETGGVLLSGMGSPAPKKIYWDHILLNASQVTNPSIDPLREPMELKTFLGRKPDKLTITGEGELEKLPP
QLELETPIMFSAMSFGSISLNACKSLARAASELGIMYNTGEGGLHRELYQYGNNTIVQVASGRFGVHKEYLEAGAAIEIK
IGQGAKPGIGGHLPGEKVGDEVSRTRMIPPGTDALSPAPHHDIYSIEDLRQLIYALKEATDYKKPVSVKISAVHNVAAIA
SGLATAGADIIAIDGFRGGTGAAPTMIRDNVGIPVELALAAVDTRLRQEGLRNRVSLVVGGSIRNSADVVKAIALGADAV
YIGSAALIALGCHMCQKCYTGKCNWGIATQRLDLVNRLNPEKGVERVKNLIRAWSHEIKEMLGGMGINAIESLRGNRLML
RGMGLNSEELKILGIKHAGQ
>Mature_499_residues
SSSHRNPEFIIERDYEKCINCRLCERQCAYEVHYYDEDQDKMMARNRNCVNCHRCVSLCPTDALGIKKYPLQFRENYNWT
SEMINNIYKQAETGGVLLSGMGSPAPKKIYWDHILLNASQVTNPSIDPLREPMELKTFLGRKPDKLTITGEGELEKLPPQ
LELETPIMFSAMSFGSISLNACKSLARAASELGIMYNTGEGGLHRELYQYGNNTIVQVASGRFGVHKEYLEAGAAIEIKI
GQGAKPGIGGHLPGEKVGDEVSRTRMIPPGTDALSPAPHHDIYSIEDLRQLIYALKEATDYKKPVSVKISAVHNVAAIAS
GLATAGADIIAIDGFRGGTGAAPTMIRDNVGIPVELALAAVDTRLRQEGLRNRVSLVVGGSIRNSADVVKAIALGADAVY
IGSAALIALGCHMCQKCYTGKCNWGIATQRLDLVNRLNPEKGVERVKNLIRAWSHEIKEMLGGMGINAIESLRGNRLMLR
GMGLNSEELKILGIKHAGQ

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0069

COG function: function code E; Glutamate synthase domain 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI308199519, Length=356, Percent_Identity=38.4831460674157, Blast_Score=206, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI17570289, Length=358, Percent_Identity=39.9441340782123, Blast_Score=215, Evalue=5e-56,
Organism=Saccharomyces cerevisiae, GI6320030, Length=333, Percent_Identity=39.6396396396396, Blast_Score=214, Evalue=2e-56,
Organism=Drosophila melanogaster, GI28574881, Length=334, Percent_Identity=41.3173652694611, Blast_Score=211, Evalue=1e-54,
Organism=Drosophila melanogaster, GI24665539, Length=334, Percent_Identity=41.3173652694611, Blast_Score=211, Evalue=1e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR013785
- InterPro:   IPR002932 [H]

Pfam domain/function: PF01645 Glu_synthase [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 54646; Mature: 54514

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSHRNPEFIIERDYEKCINCRLCERQCAYEVHYYDEDQDKMMARNRNCVNCHRCVSLC
CCCCCCCCCEEEECCHHHHHCCHHHHHHHCEEEEEECCCHHHHHHCCCCCCCHHHHHHHC
PTDALGIKKYPLQFRENYNWTSEMINNIYKQAETGGVLLSGMGSPAPKKIYWDHILLNAS
CCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEHHHHEEEHH
QVTNPSIDPLREPMELKTFLGRKPDKLTITGEGELEKLPPQLELETPIMFSAMSFGSISL
HCCCCCHHHHHCHHHHHHHHCCCCCEEEEECCCCHHHCCCCCEECCCEEEEECCCCCEEH
NACKSLARAASELGIMYNTGEGGLHRELYQYGNNTIVQVASGRFGVHKEYLEAGAAIEIK
HHHHHHHHHHHHCCEEEECCCCCHHHHHHHHCCCEEEEEECCCCCCCHHHHHCCCEEEEE
IGQGAKPGIGGHLPGEKVGDEVSRTRMIPPGTDALSPAPHHDIYSIEDLRQLIYALKEAT
ECCCCCCCCCCCCCCHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
DYKKPVSVKISAVHNVAAIASGLATAGADIIAIDGFRGGTGAAPTMIRDNVGIPVELALA
CCCCCCEEEEHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEECCCCCCHHHHHH
AVDTRLRQEGLRNRVSLVVGGSIRNSADVVKAIALGADAVYIGSAALIALGCHMCQKCYT
HHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHC
GKCNWGIATQRLDLVNRLNPEKGVERVKNLIRAWSHEIKEMLGGMGINAIESLRGNRLML
CCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEE
RGMGLNSEELKILGIKHAGQ
EECCCCCCCEEEEEECCCCC
>Mature Secondary Structure 
SSSHRNPEFIIERDYEKCINCRLCERQCAYEVHYYDEDQDKMMARNRNCVNCHRCVSLC
CCCCCCCCEEEECCHHHHHCCHHHHHHHCEEEEEECCCHHHHHHCCCCCCCHHHHHHHC
PTDALGIKKYPLQFRENYNWTSEMINNIYKQAETGGVLLSGMGSPAPKKIYWDHILLNAS
CCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEHHHHEEEHH
QVTNPSIDPLREPMELKTFLGRKPDKLTITGEGELEKLPPQLELETPIMFSAMSFGSISL
HCCCCCHHHHHCHHHHHHHHCCCCCEEEEECCCCHHHCCCCCEECCCEEEEECCCCCEEH
NACKSLARAASELGIMYNTGEGGLHRELYQYGNNTIVQVASGRFGVHKEYLEAGAAIEIK
HHHHHHHHHHHHCCEEEECCCCCHHHHHHHHCCCEEEEEECCCCCCCHHHHHCCCEEEEE
IGQGAKPGIGGHLPGEKVGDEVSRTRMIPPGTDALSPAPHHDIYSIEDLRQLIYALKEAT
ECCCCCCCCCCCCCCHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
DYKKPVSVKISAVHNVAAIASGLATAGADIIAIDGFRGGTGAAPTMIRDNVGIPVELALA
CCCCCCEEEEHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEECCCCCCHHHHHH
AVDTRLRQEGLRNRVSLVVGGSIRNSADVVKAIALGADAVYIGSAALIALGCHMCQKCYT
HHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHC
GKCNWGIATQRLDLVNRLNPEKGVERVKNLIRAWSHEIKEMLGGMGINAIESLRGNRLML
CCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEE
RGMGLNSEELKILGIKHAGQ
EECCCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]