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The map label for this gene is rpoN [H]

Identifier: 220932421

GI number: 220932421

Start: 1697994

End: 1699304

Strand: Reverse

Name: rpoN [H]

Synonym: Hore_15850

Alternate gene names: 220932421

Gene position: 1699304-1697994 (Counterclockwise)

Preceding gene: 220932422

Following gene: 220932420

Centisome position: 65.91

GC content: 37.68

Gene sequence:

>1311_bases
ATGGATTTAAGGTTTGACCTTGATCTTAATCAGAAACAGGAACTGGTGATGACCCCGAAATTGAAGCTGGCTATAAAACT
TTTGCAGTATTCTTATCAGGAACTTGAGAATTACCTTGAAGAGGAGTTAAAGCGTAACCCCCTTTTAGAGAAAGAATCTT
CAAATGGAGATTATTTTCAGAATCATAAGAATTATTCCGATTATGATTATAGTAATGACAGAAATAGTGATAATAATTAT
GAAAATTTTGTGACTTATAAAATTAATTTAACGGAATACCTCGAGTCTCAGTTGTATCAGGTCTGTGAAGATCACGAACT
TAAAATTGCAAGATATATCATCGGTAACCTGGATGACAGGGGTTATTTAAGGACGACAGTAAGTGAAATCGGTCATCATT
TTAAGGTAGAAGAAAGTGAAGTTGAGAAAGTTTTAAAGAAAGTGCAGCAGCTTGATCCAGTGGGGGTTGGGGCCCGGAAT
ATTAGAGAAGCCCTCCTGGTAAAGCTGGGAAATCTGGGAGATGATGAAAACACTGAAATAGCTAAAAATATTGTGACTAA
CTTTCTGGATGAACTGGCCTCGGGTAAAATCAAGGAGATTATAAAAAAGATTAAAAAGGATAAAGAACAGGTTTTAAAGG
CTATTAATTTAATAAAAGAGCTGGAACCCTACCCTGCTGCCGGTTTTGCTGCAAAAGAAAATACCGGTTTTATTGTCCCT
GATGTTATTGTGAGAAAGGTTGAGGGGAAATTTGTGGTTGAAATGAACAGTAGCTTAACTCCCTCATTGAAAATAAACCC
CTATTATTATAAAATGCTACAGGATGTTGGTGGGGAATCCCGGGAATACCTGGAGAAAAATTATAAGTCAGCCCTCTGGT
TAATTAGATCAATAGAACAACGGCGAATAACAATTTACCGCATAGCTATGGCTATTACCAACCGTCAACAGGAATTTCTG
GAAAAGGGTATAAAGTATTTAAAACCACTGACTATGGAAGAGCTGGCTGATACGCTGGGCATTCATGAATCCACAGTAAG
CAGGGCTACAACCAGTAAATATATTCAAACCCCACAGGGGATTTACGAACTAAAGTTTTTTTTCAGTGGTGGGGTGAATG
GGGTATCTACAACCAGTATTAAGGCCTATCTTAAGGAATATATAGATAAAGAAGGGGCAAAAAATCCCCTGAGTGATAAA
AAACTGGCCAGAATTTTGAAAGATGAAATTGGTGTTAATATTTCCAGGAGGACTGTAGCCAAGTACCGGAAGCAAATGGG
AATTCCTTCTTCCCGGAAACGGAAGAAGTAA

Upstream 100 bases:

>100_bases
ACTGGGTAAGCTACTGGACCCTCCCCTGAGTAAATCGGGGGTAAATCACCGTTTAAGGAGGATAAAATCTATTGCTAATG
AAATAAGGGGAGAGAGATAA

Downstream 100 bases:

>100_bases
TTCTCCATTTTTTTTTTAAAAAAAAGAAGGAATTTTTGAATTGACATAGTATATAATATAATTGGAAGCAAAAAGGTTAA
AAGTAAAAATAAACGACTTT

Product: RNA polymerase, sigma 54 subunit, RpoN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MDLRFDLDLNQKQELVMTPKLKLAIKLLQYSYQELENYLEEELKRNPLLEKESSNGDYFQNHKNYSDYDYSNDRNSDNNY
ENFVTYKINLTEYLESQLYQVCEDHELKIARYIIGNLDDRGYLRTTVSEIGHHFKVEESEVEKVLKKVQQLDPVGVGARN
IREALLVKLGNLGDDENTEIAKNIVTNFLDELASGKIKEIIKKIKKDKEQVLKAINLIKELEPYPAAGFAAKENTGFIVP
DVIVRKVEGKFVVEMNSSLTPSLKINPYYYKMLQDVGGESREYLEKNYKSALWLIRSIEQRRITIYRIAMAITNRQQEFL
EKGIKYLKPLTMEELADTLGIHESTVSRATTSKYIQTPQGIYELKFFFSGGVNGVSTTSIKAYLKEYIDKEGAKNPLSDK
KLARILKDEIGVNISRRTVAKYRKQMGIPSSRKRKK

Sequences:

>Translated_436_residues
MDLRFDLDLNQKQELVMTPKLKLAIKLLQYSYQELENYLEEELKRNPLLEKESSNGDYFQNHKNYSDYDYSNDRNSDNNY
ENFVTYKINLTEYLESQLYQVCEDHELKIARYIIGNLDDRGYLRTTVSEIGHHFKVEESEVEKVLKKVQQLDPVGVGARN
IREALLVKLGNLGDDENTEIAKNIVTNFLDELASGKIKEIIKKIKKDKEQVLKAINLIKELEPYPAAGFAAKENTGFIVP
DVIVRKVEGKFVVEMNSSLTPSLKINPYYYKMLQDVGGESREYLEKNYKSALWLIRSIEQRRITIYRIAMAITNRQQEFL
EKGIKYLKPLTMEELADTLGIHESTVSRATTSKYIQTPQGIYELKFFFSGGVNGVSTTSIKAYLKEYIDKEGAKNPLSDK
KLARILKDEIGVNISRRTVAKYRKQMGIPSSRKRKK
>Mature_436_residues
MDLRFDLDLNQKQELVMTPKLKLAIKLLQYSYQELENYLEEELKRNPLLEKESSNGDYFQNHKNYSDYDYSNDRNSDNNY
ENFVTYKINLTEYLESQLYQVCEDHELKIARYIIGNLDDRGYLRTTVSEIGHHFKVEESEVEKVLKKVQQLDPVGVGARN
IREALLVKLGNLGDDENTEIAKNIVTNFLDELASGKIKEIIKKIKKDKEQVLKAINLIKELEPYPAAGFAAKENTGFIVP
DVIVRKVEGKFVVEMNSSLTPSLKINPYYYKMLQDVGGESREYLEKNYKSALWLIRSIEQRRITIYRIAMAITNRQQEFL
EKGIKYLKPLTMEELADTLGIHESTVSRATTSKYIQTPQGIYELKFFFSGGVNGVSTTSIKAYLKEYIDKEGAKNPLSDK
KLARILKDEIGVNISRRTVAKYRKQMGIPSSRKRKK

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the nitrogen fixation genes. The open complex (sigma-54 and core

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=477, Percent_Identity=35.6394129979036, Blast_Score=278, Evalue=5e-76,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 50439; Mature: 50439

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLRFDLDLNQKQELVMTPKLKLAIKLLQYSYQELENYLEEELKRNPLLEKESSNGDYFQ
CCEEECCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
NHKNYSDYDYSNDRNSDNNYENFVTYKINLTEYLESQLYQVCEDHELKIARYIIGNLDDR
CCCCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCC
GYLRTTVSEIGHHFKVEESEVEKVLKKVQQLDPVGVGARNIREALLVKLGNLGDDENTEI
CHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCHHHH
AKNIVTNFLDELASGKIKEIIKKIKKDKEQVLKAINLIKELEPYPAAGFAAKENTGFIVP
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCH
DVIVRKVEGKFVVEMNSSLTPSLKINPYYYKMLQDVGGESREYLEKNYKSALWLIRSIEQ
HHHHHHHCCCEEEEECCCCCCCEEECHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
RRITIYRIAMAITNRQQEFLEKGIKYLKPLTMEELADTLGIHESTVSRATTSKYIQTPQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCC
IYELKFFFSGGVNGVSTTSIKAYLKEYIDKEGAKNPLSDKKLARILKDEIGVNISRRTVA
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHH
KYRKQMGIPSSRKRKK
HHHHHCCCCCCCCCCC
>Mature Secondary Structure
MDLRFDLDLNQKQELVMTPKLKLAIKLLQYSYQELENYLEEELKRNPLLEKESSNGDYFQ
CCEEECCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
NHKNYSDYDYSNDRNSDNNYENFVTYKINLTEYLESQLYQVCEDHELKIARYIIGNLDDR
CCCCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCC
GYLRTTVSEIGHHFKVEESEVEKVLKKVQQLDPVGVGARNIREALLVKLGNLGDDENTEI
CHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCHHHH
AKNIVTNFLDELASGKIKEIIKKIKKDKEQVLKAINLIKELEPYPAAGFAAKENTGFIVP
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCH
DVIVRKVEGKFVVEMNSSLTPSLKINPYYYKMLQDVGGESREYLEKNYKSALWLIRSIEQ
HHHHHHHCCCEEEEECCCCCCCEEECHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
RRITIYRIAMAITNRQQEFLEKGIKYLKPLTMEELADTLGIHESTVSRATTSKYIQTPQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCC
IYELKFFFSGGVNGVSTTSIKAYLKEYIDKEGAKNPLSDKKLARILKDEIGVNISRRTVA
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHH
KYRKQMGIPSSRKRKK
HHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2999700; 2695747 [H]