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The map label for this gene is pleD [H]

Identifier: 220932323

GI number: 220932323

Start: 1583941

End: 1585329

Strand: Reverse

Name: pleD [H]

Synonym: Hore_14870

Alternate gene names: 220932323

Gene position: 1585329-1583941 (Counterclockwise)

Preceding gene: 220932325

Following gene: 220932321

Centisome position: 61.49

GC content: 35.71

Gene sequence:

>1389_bases
ATGAGACTCTCAAAAAAAATTAAAATAAATGAGAAACTCATGGTGCTGGTCGGGTTATTTTATCTGGCCTTTACCGGAAG
TTTGTTTTATCTGCCCTTTACTCCAGGGTTATTTCTTTTTTTATTTGTACCGGTCGTTTACCTTGATATTGTTATCGGTT
TAAAAGCTGCCCTGGTATATAACACAATTTTTAATTTGTTATTTATTGTTTATGAAGGGGTAGAGTATAACTTTGCATTT
CCGGGAAAGGTTGCTGTTTTTTTGATTCTGGGTTGTGCCTTTACCTATTTTATTCTTTATTTGATAAGCCACTTTTGTGA
GAAATATAAAAGTAAACAGAGGGATTTAAAAGAGTCTATGGCCCAGCTGGCTGCTTCATACCGTACCCTCGAATCTACCC
AGACTAAATTGTTTGCCCTGGCCCGGATTACCAAAGAATTACTTGCCATTCATGACCATAAAGAGTTGATTCCTGTCCTT
ATAAATTTATTAAACAGTCATTTTTTCTATTACAACATTGCCTATGTCAGTAAGAATAAAAACAGTGAGATTAATTTTAC
CATGGAGTCAAGAATCGGGTTTACCGGTATTGATAACAGGCATTTAATAAATATTGTAAATGAATATACACCCTTTGATG
ATGTCCTTATAAAAACTGTAGATAGAGATGAAATTATCAAAACGACCGACCGGGTCTGTGCTGATACTTCTAAACGAGGG
AAACTTATTTTAGTTCCGGTTGTAACAAATGATGATATTAAGGGAATATTCCTGATATACAATGAGGGTGACTGGGTGGA
GGAAGAGGACCGGGATATTTTGAGGGTTCTGGCAGATCAGACCGGGTTATTACTCAATAAACTGGAGTTAATTGAAGAAA
TCAATTACCTGGCAATTACTGATGAAGGAACAGATCTATATAACCAGCGGTATTTCTATAAAAGGCTCAATAGATTATTT
TCTTATGCTAAAAAGAATAATAAACCCTTATCAGTGGTTATTTTTGATATTGATAACTTTAAGTCATTGAATGACAGCTA
TGGACACCTTTTCGGGGATAAGGTTTTAAGAGAAGTAGCTTCCCTTCTCCAGGATAATATACGGAAAAACGATATACTGG
CCCGGTATGGGGGAGAAGAATTTGCCTTAATATTACCAAAGACTGGAGCCAGTATAGCCTATAATATTGCGGAAAGAATA
AGGTGCATTGTTAATAAACATATCTTTGAGACTGGTGGGGGACATAAGATTAAAATAAGTATTTCGGGAGGGATAGCAAC
ATATCCTGAGTGTAAGGTTTCGAAAGCCATTGAGCTTATAGACCTGGCAGATAAGGCCCTATATAAATCCAAAGATGACG
GTAAAAACATGATTAATTCTGTTAGATAA

Upstream 100 bases:

>100_bases
TATATGGGTCCTGTCGTTTATTTAAAACCTGGACTTTCACCTGAATTATTTTACTAAAATTATTAAAGAAAGACTGATAG
CCTTGAAGAAAATTAATTTT

Downstream 100 bases:

>100_bases
AGAAAGCCTCCTTCTTGACAGGAAGGAGGCTTATATTAATTAACTTGATATTCATTAACTTGAGATATTTGTGTTATAGA
TTTAGTTGAAAGAGGGTTGT

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MRLSKKIKINEKLMVLVGLFYLAFTGSLFYLPFTPGLFLFLFVPVVYLDIVIGLKAALVYNTIFNLLFIVYEGVEYNFAF
PGKVAVFLILGCAFTYFILYLISHFCEKYKSKQRDLKESMAQLAASYRTLESTQTKLFALARITKELLAIHDHKELIPVL
INLLNSHFFYYNIAYVSKNKNSEINFTMESRIGFTGIDNRHLINIVNEYTPFDDVLIKTVDRDEIIKTTDRVCADTSKRG
KLILVPVVTNDDIKGIFLIYNEGDWVEEEDRDILRVLADQTGLLLNKLELIEEINYLAITDEGTDLYNQRYFYKRLNRLF
SYAKKNNKPLSVVIFDIDNFKSLNDSYGHLFGDKVLREVASLLQDNIRKNDILARYGGEEFALILPKTGASIAYNIAERI
RCIVNKHIFETGGGHKIKISISGGIATYPECKVSKAIELIDLADKALYKSKDDGKNMINSVR

Sequences:

>Translated_462_residues
MRLSKKIKINEKLMVLVGLFYLAFTGSLFYLPFTPGLFLFLFVPVVYLDIVIGLKAALVYNTIFNLLFIVYEGVEYNFAF
PGKVAVFLILGCAFTYFILYLISHFCEKYKSKQRDLKESMAQLAASYRTLESTQTKLFALARITKELLAIHDHKELIPVL
INLLNSHFFYYNIAYVSKNKNSEINFTMESRIGFTGIDNRHLINIVNEYTPFDDVLIKTVDRDEIIKTTDRVCADTSKRG
KLILVPVVTNDDIKGIFLIYNEGDWVEEEDRDILRVLADQTGLLLNKLELIEEINYLAITDEGTDLYNQRYFYKRLNRLF
SYAKKNNKPLSVVIFDIDNFKSLNDSYGHLFGDKVLREVASLLQDNIRKNDILARYGGEEFALILPKTGASIAYNIAERI
RCIVNKHIFETGGGHKIKISISGGIATYPECKVSKAIELIDLADKALYKSKDDGKNMINSVR
>Mature_462_residues
MRLSKKIKINEKLMVLVGLFYLAFTGSLFYLPFTPGLFLFLFVPVVYLDIVIGLKAALVYNTIFNLLFIVYEGVEYNFAF
PGKVAVFLILGCAFTYFILYLISHFCEKYKSKQRDLKESMAQLAASYRTLESTQTKLFALARITKELLAIHDHKELIPVL
INLLNSHFFYYNIAYVSKNKNSEINFTMESRIGFTGIDNRHLINIVNEYTPFDDVLIKTVDRDEIIKTTDRVCADTSKRG
KLILVPVVTNDDIKGIFLIYNEGDWVEEEDRDILRVLADQTGLLLNKLELIEEINYLAITDEGTDLYNQRYFYKRLNRLF
SYAKKNNKPLSVVIFDIDNFKSLNDSYGHLFGDKVLREVASLLQDNIRKNDILARYGGEEFALILPKTGASIAYNIAERI
RCIVNKHIFETGGGHKIKISISGGIATYPECKVSKAIELIDLADKALYKSKDDGKNMINSVR

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=174, Percent_Identity=36.7816091954023, Blast_Score=115, Evalue=8e-27,
Organism=Escherichia coli, GI87082007, Length=169, Percent_Identity=34.3195266272189, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1788381, Length=172, Percent_Identity=36.6279069767442, Blast_Score=105, Evalue=8e-24,
Organism=Escherichia coli, GI1787262, Length=166, Percent_Identity=36.7469879518072, Blast_Score=101, Evalue=8e-23,
Organism=Escherichia coli, GI1786584, Length=165, Percent_Identity=31.5151515151515, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI87081881, Length=170, Percent_Identity=36.4705882352941, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788085, Length=200, Percent_Identity=32, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787816, Length=180, Percent_Identity=33.3333333333333, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1787541, Length=166, Percent_Identity=34.3373493975904, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787802, Length=177, Percent_Identity=32.7683615819209, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1787056, Length=130, Percent_Identity=36.1538461538462, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI87081974, Length=111, Percent_Identity=30.6306306306306, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788956, Length=155, Percent_Identity=32.258064516129, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI87081977, Length=145, Percent_Identity=30.3448275862069, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 52902; Mature: 52902

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSKKIKINEKLMVLVGLFYLAFTGSLFYLPFTPGLFLFLFVPVVYLDIVIGLKAALVY
CCCCCCCCCCHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
NTIFNLLFIVYEGVEYNFAFPGKVAVFLILGCAFTYFILYLISHFCEKYKSKQRDLKESM
HHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQLAASYRTLESTQTKLFALARITKELLAIHDHKELIPVLINLLNSHFFYYNIAYVSKNK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCEEEEEEEEEECCC
NSEINFTMESRIGFTGIDNRHLINIVNEYTPFDDVLIKTVDRDEIIKTTDRVCADTSKRG
CCEEEEEECCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCC
KLILVPVVTNDDIKGIFLIYNEGDWVEEEDRDILRVLADQTGLLLNKLELIEEINYLAIT
CEEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
DEGTDLYNQRYFYKRLNRLFSYAKKNNKPLSVVIFDIDNFKSLNDSYGHLFGDKVLREVA
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
SLLQDNIRKNDILARYGGEEFALILPKTGASIAYNIAERIRCIVNKHIFETGGGHKIKIS
HHHHHCCCCCCCCEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
ISGGIATYPECKVSKAIELIDLADKALYKSKDDGKNMINSVR
ECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCC
>Mature Secondary Structure
MRLSKKIKINEKLMVLVGLFYLAFTGSLFYLPFTPGLFLFLFVPVVYLDIVIGLKAALVY
CCCCCCCCCCHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
NTIFNLLFIVYEGVEYNFAFPGKVAVFLILGCAFTYFILYLISHFCEKYKSKQRDLKESM
HHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQLAASYRTLESTQTKLFALARITKELLAIHDHKELIPVLINLLNSHFFYYNIAYVSKNK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCEEEEEEEEEECCC
NSEINFTMESRIGFTGIDNRHLINIVNEYTPFDDVLIKTVDRDEIIKTTDRVCADTSKRG
CCEEEEEECCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCC
KLILVPVVTNDDIKGIFLIYNEGDWVEEEDRDILRVLADQTGLLLNKLELIEEINYLAIT
CEEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
DEGTDLYNQRYFYKRLNRLFSYAKKNNKPLSVVIFDIDNFKSLNDSYGHLFGDKVLREVA
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
SLLQDNIRKNDILARYGGEEFALILPKTGASIAYNIAERIRCIVNKHIFETGGGHKIKIS
HHHHHCCCCCCCCEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
ISGGIATYPECKVSKAIELIDLADKALYKSKDDGKNMINSVR
ECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA