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The map label for this gene is apbE [H]

Identifier: 220932264

GI number: 220932264

Start: 1514931

End: 1515941

Strand: Reverse

Name: apbE [H]

Synonym: Hore_14280

Alternate gene names: 220932264

Gene position: 1515941-1514931 (Counterclockwise)

Preceding gene: 220932265

Following gene: 220932263

Centisome position: 58.8

GC content: 41.15

Gene sequence:

>1011_bases
ATGGGGTTTCCTGGTAAGTATTTTCTAAGGGTAATTATTCTTTTCATTTTATTAATAATTGCTGTAATTATGGTTAAACT
TTTTGCCCGACCAGAAATAATTTCCCGGACAGATTATTTCATGGATACAACAGTTACTATTAAAGTAGTAAAACAACCCG
GAGCCGGGGATATTATAGAAGAAGCTCTCTCATATATGAAGGAGAAAGCCGACAGGTTTGACAGGTTTAACCCTGAAAGT
GATATTTCTGTAATAAATCGAAATAGTGGTCAGGCTGTTAAAGTAAATGAAGATACCGTTGAATTATTGAGATATTCTTT
AAAATATGCCCGGATGAGCCAGGGGGCTTTTGACCCTACTGTTGCCCCCCTGATGGACCTCTGGGGTTTCAGTAAAAACA
GGAAAAACCGGGTACCTTCACCTGGAGAGATTAAAGGGGTATTATCCAGAGTGGGGTATGACAGGATAGAGATTTACCGT
AGTAACCAGGTTTATTTACCTCCAGGAATGGCCATAGATCTGGGTGGTCTGGCCAAAGGATATGTAGTCGATGAAGGAAT
TAAATTTTTAAGAGATAATGGAATAAAAGCCGGAATGATAAATGCCGGGGGAAATATTAGAGTAACCGGGGTGAAATCCC
CGGGTATTCCCTGGAAGTTCGGGATAAGAGACCCTGAAGATAGGGGGCAAATACTAAAAGGGTATATAATAAACCTGAAA
AGGGGTAGTCTGGCTACTTCCGGTGATTATGAGAGATTTTTTGTGGCTAACGGGCATCGTTATTCCCATCTTCTTGACCC
CCGGACCGGTTACCCCGGTTCAGATCTTCAGTCTGTGACCATATATGCACCAACGGCCTTAGAAGCAGATATTTTGTCAA
CTGCTGTTTTTATAATGGGCTGGGAAAGGGGACGGGAATTAATTGAAAAAATCCCCGATGTTGAAGGGCTTATGATTAAA
AACGACCAGATCTGGTTGAGTAAAGGATTCAGGGAGTTAATTAACAGGTAA

Upstream 100 bases:

>100_bases
ATATGTGAAAAAACAGGCTGGATAAAAGGGCCTGGGCCTGTTATTGTATGTGTTCCCAACAAAATATCGATATGGATTGA
GTCAAAGGAGACAGAAATTG

Downstream 100 bases:

>100_bases
ATTTTACCCTTTCTGGATATTAAGAAGGAAATATAGTACCCTGTATTGAATTATAAATCAGGGTCTTTAGTAATTGATTT
TTTTGTCTACAAATAGTACT

Product: ApbE family lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MGFPGKYFLRVIILFILLIIAVIMVKLFARPEIISRTDYFMDTTVTIKVVKQPGAGDIIEEALSYMKEKADRFDRFNPES
DISVINRNSGQAVKVNEDTVELLRYSLKYARMSQGAFDPTVAPLMDLWGFSKNRKNRVPSPGEIKGVLSRVGYDRIEIYR
SNQVYLPPGMAIDLGGLAKGYVVDEGIKFLRDNGIKAGMINAGGNIRVTGVKSPGIPWKFGIRDPEDRGQILKGYIINLK
RGSLATSGDYERFFVANGHRYSHLLDPRTGYPGSDLQSVTIYAPTALEADILSTAVFIMGWERGRELIEKIPDVEGLMIK
NDQIWLSKGFRELINR

Sequences:

>Translated_336_residues
MGFPGKYFLRVIILFILLIIAVIMVKLFARPEIISRTDYFMDTTVTIKVVKQPGAGDIIEEALSYMKEKADRFDRFNPES
DISVINRNSGQAVKVNEDTVELLRYSLKYARMSQGAFDPTVAPLMDLWGFSKNRKNRVPSPGEIKGVLSRVGYDRIEIYR
SNQVYLPPGMAIDLGGLAKGYVVDEGIKFLRDNGIKAGMINAGGNIRVTGVKSPGIPWKFGIRDPEDRGQILKGYIINLK
RGSLATSGDYERFFVANGHRYSHLLDPRTGYPGSDLQSVTIYAPTALEADILSTAVFIMGWERGRELIEKIPDVEGLMIK
NDQIWLSKGFRELINR
>Mature_335_residues
GFPGKYFLRVIILFILLIIAVIMVKLFARPEIISRTDYFMDTTVTIKVVKQPGAGDIIEEALSYMKEKADRFDRFNPESD
ISVINRNSGQAVKVNEDTVELLRYSLKYARMSQGAFDPTVAPLMDLWGFSKNRKNRVPSPGEIKGVLSRVGYDRIEIYRS
NQVYLPPGMAIDLGGLAKGYVVDEGIKFLRDNGIKAGMINAGGNIRVTGVKSPGIPWKFGIRDPEDRGQILKGYIINLKR
GSLATSGDYERFFVANGHRYSHLLDPRTGYPGSDLQSVTIYAPTALEADILSTAVFIMGWERGRELIEKIPDVEGLMIKN
DQIWLSKGFRELINR

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=270, Percent_Identity=30, Blast_Score=141, Evalue=7e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 37721; Mature: 37590

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFPGKYFLRVIILFILLIIAVIMVKLFARPEIISRTDYFMDTTVTIKVVKQPGAGDIIE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCEECCEEEEEEEECCCCCHHHH
EALSYMKEKADRFDRFNPESDISVINRNSGQAVKVNEDTVELLRYSLKYARMSQGAFDPT
HHHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEEECHHHHHHHHHHHHHHHHCCCCCCCH
VAPLMDLWGFSKNRKNRVPSPGEIKGVLSRVGYDRIEIYRSNQVYLPPGMAIDLGGLAKG
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCEEEECCCCEEECCCCCCC
YVVDEGIKFLRDNGIKAGMINAGGNIRVTGVKSPGIPWKFGIRDPEDRGQILKGYIINLK
CCHHHHHHHHHHCCCEEEEEECCCEEEEEEECCCCCCEEECCCCCHHHHHEEEEEEEEEE
RGSLATSGDYERFFVANGHRYSHLLDPRTGYPGSDLQSVTIYAPTALEADILSTAVFIMG
CCCCCCCCCCCEEEEECCCCCCEECCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHH
WERGRELIEKIPDVEGLMIKNDQIWLSKGFRELINR
HHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHCC
>Mature Secondary Structure 
GFPGKYFLRVIILFILLIIAVIMVKLFARPEIISRTDYFMDTTVTIKVVKQPGAGDIIE
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCEECCEEEEEEEECCCCCHHHH
EALSYMKEKADRFDRFNPESDISVINRNSGQAVKVNEDTVELLRYSLKYARMSQGAFDPT
HHHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEEECHHHHHHHHHHHHHHHHCCCCCCCH
VAPLMDLWGFSKNRKNRVPSPGEIKGVLSRVGYDRIEIYRSNQVYLPPGMAIDLGGLAKG
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCEEEECCCCEEECCCCCCC
YVVDEGIKFLRDNGIKAGMINAGGNIRVTGVKSPGIPWKFGIRDPEDRGQILKGYIINLK
CCHHHHHHHHHHCCCEEEEEECCCEEEEEEECCCCCCEEECCCCCHHHHHEEEEEEEEEE
RGSLATSGDYERFFVANGHRYSHLLDPRTGYPGSDLQSVTIYAPTALEADILSTAVFIMG
CCCCCCCCCCCEEEEECCCCCCEECCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHH
WERGRELIEKIPDVEGLMIKNDQIWLSKGFRELINR
HHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]