| Definition | Halothermothrix orenii H 168 chromosome, complete genome. |
|---|---|
| Accession | NC_011899 |
| Length | 2,578,146 |
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The map label for this gene is pulA [H]
Identifier: 220932225
GI number: 220932225
Start: 1479115
End: 1481037
Strand: Reverse
Name: pulA [H]
Synonym: Hore_13880
Alternate gene names: 220932225
Gene position: 1481037-1479115 (Counterclockwise)
Preceding gene: 220932227
Following gene: 220932224
Centisome position: 57.45
GC content: 38.69
Gene sequence:
>1923_bases ATGAAAAAAAATAAAAAGGATTTCGAAACGTCAACTGAAATAGCAGTCAAAAAAAAGGGTTTTTACTACGGTGGGGATGA TCTCGGGGTTTTATACACCCCTTCAAGGACTACCTTCAGGTTCTGGTCTCCCCTGGCCAGGTGGGCTAAAGTTTATATTT TTTCTAATGAAAATTCTGAAAACCCTGAGATTGTTTCTGACTTCAAAAAAGAAGCCGGGGGTAGCTGGGTTGCTGAAGTT TCAGGTGATTTGGAAGGCAAGTATTATATTTATGAACTAGAGGTTAATGGCAAAGTTAATAGAGTAGTGGACCCCTATGC CAGGGCTTTAAGCACCAATAGTAAGAGGGGATTAATTGTTAATATGGATAAAACAAACCCTCCGGGGTGGGAAAAAGATG AGAGGGTAACCTTACAAAAACCCCAGGATGCAATAATTTATGAAGTTCATGTCAGGGATTTTTCATCTTCCCCCCATTCG GGTATTGTAAATAAAGGTAAATATCTTGCTTTTACTGAAGGGGGTACTACTGGTCCTGGTGGGGTGAAAACCGGGCTAGA TCACTTAAAGGAACTGGGCGTTACCCATGTTCATTTATTACCCGTCTTTGATTTTGGAAGTGTTGATGATCTTAATGATA AAGAGTATAACTGGGGTTATGACCCCCTGTTTTATAATACACCGGAAGGTTCATATTCCACCAACCCTTCAGATGACTCC CGTATAAAAGAATTTAAGCAGTTAGTAAAATCTTTACACGAAAATGGTATCGGGGTTATTATGGATGTTGTTTACAACCA CACCTACTATACGCGCAAATCGGCCTTTGATTTAATTGTACCTCAGTATTATTACAGGTTTGACCATGATGGGAATTATT CCAATGGGTCAGGATGTGGTAATGAGGTTGCTTCTGAAAAGCCAATGGTTAGAAAATTTATTATTGATTCTGTTAAATAC TGGGCTGAGGAATACCATATAGATGGTTTCAGGTTTGACCTTATGGCTTTAATTGATCGGGAAACTATAAAACATATAGA ACATACATTACATGAAATTGATCCTTCGATTATTATATATGGTGAACCCTGGACCGGGGGATTGACAACCCTTGATCCAG AAAGACAGTTGCTTAAAGGGGCCCAGAAGGGTATGAAAGTTGCTGTTTTTAATGATAATTTCAGGAATGCTATAAAAGGT GATAACGATGGTTTTGGTAAGGGTTTTGCCTCCGGAGCCCCGGGTTTAGAGCATGCCATAAAAAGGGGTGTCGTCGGGGC TATATATTATAATGATGAGATAAATGATTTCTCCTCAGAACCCCGGGAGACAGTTAATTATGTTTCCTCCCATGATAACT TAACCCTCTGGGATAAACTGTGTAAGTCCAACGGTGAAGATGAAGAATGGGTAAGAATAAGGATGGACCATTTAAGCCAG GCTATAATTTTTACTTCCCAGGGTGTGGCTTTTATACAGGGTGGGGAGGAATTTTTGAGAACCAAATTTGGCAACCATAA TAGTTATAATGCCGGTGATGAAGTAAACCAGCTCAAGTGGGAACGGAAAAGTAAGTATCTTAAAACATTTAAGTATTATA AAGGTTTAATTACTTTAAGAAGAGAACATCCGGCTTTCAGGATGACAAATGCTGAACAAATCAGGAAAAAACTTGAGTTT TTAGAAACACTTCCAAATACGGTAGGGTTTATATTAAAAGATTATGCTAACGGGGATAACTGGAAAAACATAGTGGTACT CTATAACCCTAACAGGGAAGCAATAACCTTTAACTTGCCCTATGAGGGAGCATGGAATGTAGTAGTGTATGAAGAAGAAG CCGGTACCGATACTATTTTTACTGTAAAAAATGGAGAAGTAAAGGTACCCCTTATTAGTACAGTTGTACTTTATCAAGAT TAG
Upstream 100 bases:
>100_bases AAAAGGTGAAGATAAGAATAGACCGGAAATATTAGTTTAATATTTAGTATTTTTAAATTTTTTATATTATAGGACTCTTG TGGGAAGGAGAGGTACATTA
Downstream 100 bases:
>100_bases TTGCCAGCCCGATTAGGTATTTTATCTACTGGAATATTTTTTCTATTTTTGGAGGATGTTAAATATTTATGTAGAATTAT ATAACTAAAGTTATTATAAA
Product: pullulanase, type I
Products: NA
Alternate protein names: Alpha-dextrin endo-1,6-alpha-glucosidase; Pullulan 6-glucanohydrolase [H]
Number of amino acids: Translated: 640; Mature: 640
Protein sequence:
>640_residues MKKNKKDFETSTEIAVKKKGFYYGGDDLGVLYTPSRTTFRFWSPLARWAKVYIFSNENSENPEIVSDFKKEAGGSWVAEV SGDLEGKYYIYELEVNGKVNRVVDPYARALSTNSKRGLIVNMDKTNPPGWEKDERVTLQKPQDAIIYEVHVRDFSSSPHS GIVNKGKYLAFTEGGTTGPGGVKTGLDHLKELGVTHVHLLPVFDFGSVDDLNDKEYNWGYDPLFYNTPEGSYSTNPSDDS RIKEFKQLVKSLHENGIGVIMDVVYNHTYYTRKSAFDLIVPQYYYRFDHDGNYSNGSGCGNEVASEKPMVRKFIIDSVKY WAEEYHIDGFRFDLMALIDRETIKHIEHTLHEIDPSIIIYGEPWTGGLTTLDPERQLLKGAQKGMKVAVFNDNFRNAIKG DNDGFGKGFASGAPGLEHAIKRGVVGAIYYNDEINDFSSEPRETVNYVSSHDNLTLWDKLCKSNGEDEEWVRIRMDHLSQ AIIFTSQGVAFIQGGEEFLRTKFGNHNSYNAGDEVNQLKWERKSKYLKTFKYYKGLITLRREHPAFRMTNAEQIRKKLEF LETLPNTVGFILKDYANGDNWKNIVVLYNPNREAITFNLPYEGAWNVVVYEEEAGTDTIFTVKNGEVKVPLISTVVLYQD
Sequences:
>Translated_640_residues MKKNKKDFETSTEIAVKKKGFYYGGDDLGVLYTPSRTTFRFWSPLARWAKVYIFSNENSENPEIVSDFKKEAGGSWVAEV SGDLEGKYYIYELEVNGKVNRVVDPYARALSTNSKRGLIVNMDKTNPPGWEKDERVTLQKPQDAIIYEVHVRDFSSSPHS GIVNKGKYLAFTEGGTTGPGGVKTGLDHLKELGVTHVHLLPVFDFGSVDDLNDKEYNWGYDPLFYNTPEGSYSTNPSDDS RIKEFKQLVKSLHENGIGVIMDVVYNHTYYTRKSAFDLIVPQYYYRFDHDGNYSNGSGCGNEVASEKPMVRKFIIDSVKY WAEEYHIDGFRFDLMALIDRETIKHIEHTLHEIDPSIIIYGEPWTGGLTTLDPERQLLKGAQKGMKVAVFNDNFRNAIKG DNDGFGKGFASGAPGLEHAIKRGVVGAIYYNDEINDFSSEPRETVNYVSSHDNLTLWDKLCKSNGEDEEWVRIRMDHLSQ AIIFTSQGVAFIQGGEEFLRTKFGNHNSYNAGDEVNQLKWERKSKYLKTFKYYKGLITLRREHPAFRMTNAEQIRKKLEF LETLPNTVGFILKDYANGDNWKNIVVLYNPNREAITFNLPYEGAWNVVVYEEEAGTDTIFTVKNGEVKVPLISTVVLYQD >Mature_640_residues MKKNKKDFETSTEIAVKKKGFYYGGDDLGVLYTPSRTTFRFWSPLARWAKVYIFSNENSENPEIVSDFKKEAGGSWVAEV SGDLEGKYYIYELEVNGKVNRVVDPYARALSTNSKRGLIVNMDKTNPPGWEKDERVTLQKPQDAIIYEVHVRDFSSSPHS GIVNKGKYLAFTEGGTTGPGGVKTGLDHLKELGVTHVHLLPVFDFGSVDDLNDKEYNWGYDPLFYNTPEGSYSTNPSDDS RIKEFKQLVKSLHENGIGVIMDVVYNHTYYTRKSAFDLIVPQYYYRFDHDGNYSNGSGCGNEVASEKPMVRKFIIDSVKY WAEEYHIDGFRFDLMALIDRETIKHIEHTLHEIDPSIIIYGEPWTGGLTTLDPERQLLKGAQKGMKVAVFNDNFRNAIKG DNDGFGKGFASGAPGLEHAIKRGVVGAIYYNDEINDFSSEPRETVNYVSSHDNLTLWDKLCKSNGEDEEWVRIRMDHLSQ AIIFTSQGVAFIQGGEEFLRTKFGNHNSYNAGDEVNQLKWERKSKYLKTFKYYKGLITLRREHPAFRMTNAEQIRKKLEF LETLPNTVGFILKDYANGDNWKNIVVLYNPNREAITFNLPYEGAWNVVVYEEEAGTDTIFTVKNGEVKVPLISTVVLYQD
Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]
COG id: COG1523
COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the glycosyl hydrolase 13 family [H]
Homologues:
Organism=Escherichia coli, GI2367229, Length=454, Percent_Identity=27.3127753303965, Blast_Score=142, Evalue=6e-35,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR006047 - InterPro: IPR004193 - InterPro: IPR017853 - InterPro: IPR013781 - InterPro: IPR013783 - InterPro: IPR014756 - InterPro: IPR005323 - InterPro: IPR011840 [H]
Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48; PF03714 PUD [H]
EC number: =3.2.1.41 [H]
Molecular weight: Translated: 72953; Mature: 72953
Theoretical pI: Translated: 5.98; Mature: 5.98
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.3 %Cys (Translated Protein) 1.2 %Met (Translated Protein) 1.6 %Cys+Met (Translated Protein) 0.3 %Cys (Mature Protein) 1.2 %Met (Mature Protein) 1.6 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKKNKKDFETSTEIAVKKKGFYYGGDDLGVLYTPSRTTFRFWSPLARWAKVYIFSNENSE CCCCCCCCCCCCCEEEEECCCEECCCCEEEEECCCCCCCHHHHHHHCEEEEEEEECCCCC NPEIVSDFKKEAGGSWVAEVSGDLEGKYYIYELEVNGKVNRVVDPYARALSTNSKRGLIV CCHHHHHHHHHCCCCEEEEECCCCCCEEEEEEEEECCCHHHHHHHHHHHHCCCCCCCEEE NMDKTNPPGWEKDERVTLQKPQDAIIYEVHVRDFSSSPHSGIVNKGKYLAFTEGGTTGPG EECCCCCCCCCCCCCEEEECCCCEEEEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCC GVKTGLDHLKELGVTHVHLLPVFDFGSVDDLNDKEYNWGYDPLFYNTPEGSYSTNPSDDS HHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCHH RIKEFKQLVKSLHENGIGVIMDVVYNHTYYTRKSAFDLIVPQYYYRFDHDGNYSNGSGCG HHHHHHHHHHHHHHCCCEEEEHHHHCCCEEECCCCHHEECCCHHEEECCCCCCCCCCCCC NEVASEKPMVRKFIIDSVKYWAEEYHIDGFRFDLMALIDRETIKHIEHTLHEIDPSIIIY CHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE GEPWTGGLTTLDPERQLLKGAQKGMKVAVFNDNFRNAIKGDNDGFGKGFASGAPGLEHAI CCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCHHHCCCCCCCCCCCCCCCCCCHHHHH KRGVVGAIYYNDEINDFSSEPRETVNYVSSHDNLTLWDKLCKSNGEDEEWVRIRMDHLSQ HCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHEHHHHCE AIIFTSQGVAFIQGGEEFLRTKFGNHNSYNAGDEVNQLKWERKSKYLKTFKYYKGLITLR EEEEECCCEEEEECCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEE REHPAFRMTNAEQIRKKLEFLETLPNTVGFILKDYANGDNWKNIVVLYNPNREAITFNLP CCCCCEECCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEEEEECCCCCEEEEECC YEGAWNVVVYEEEAGTDTIFTVKNGEVKVPLISTVVLYQD CCCCEEEEEEECCCCCCEEEEEECCEEEEEEEEEEEEEEC >Mature Secondary Structure MKKNKKDFETSTEIAVKKKGFYYGGDDLGVLYTPSRTTFRFWSPLARWAKVYIFSNENSE CCCCCCCCCCCCCEEEEECCCEECCCCEEEEECCCCCCCHHHHHHHCEEEEEEEECCCCC NPEIVSDFKKEAGGSWVAEVSGDLEGKYYIYELEVNGKVNRVVDPYARALSTNSKRGLIV CCHHHHHHHHHCCCCEEEEECCCCCCEEEEEEEEECCCHHHHHHHHHHHHCCCCCCCEEE NMDKTNPPGWEKDERVTLQKPQDAIIYEVHVRDFSSSPHSGIVNKGKYLAFTEGGTTGPG EECCCCCCCCCCCCCEEEECCCCEEEEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCC GVKTGLDHLKELGVTHVHLLPVFDFGSVDDLNDKEYNWGYDPLFYNTPEGSYSTNPSDDS HHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCHH RIKEFKQLVKSLHENGIGVIMDVVYNHTYYTRKSAFDLIVPQYYYRFDHDGNYSNGSGCG HHHHHHHHHHHHHHCCCEEEEHHHHCCCEEECCCCHHEECCCHHEEECCCCCCCCCCCCC NEVASEKPMVRKFIIDSVKYWAEEYHIDGFRFDLMALIDRETIKHIEHTLHEIDPSIIIY CHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE GEPWTGGLTTLDPERQLLKGAQKGMKVAVFNDNFRNAIKGDNDGFGKGFASGAPGLEHAI CCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCHHHCCCCCCCCCCCCCCCCCCHHHHH KRGVVGAIYYNDEINDFSSEPRETVNYVSSHDNLTLWDKLCKSNGEDEEWVRIRMDHLSQ HCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHEHHHHCE AIIFTSQGVAFIQGGEEFLRTKFGNHNSYNAGDEVNQLKWERKSKYLKTFKYYKGLITLR EEEEECCCEEEEECCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEE REHPAFRMTNAEQIRKKLEFLETLPNTVGFILKDYANGDNWKNIVVLYNPNREAITFNLP CCCCCEECCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEEEEECCCCCEEEEECC YEGAWNVVVYEEEAGTDTIFTVKNGEVKVPLISTVVLYQD CCCCEEEEEEECCCCCCEEEEEECCEEEEEEEEEEEEEEC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9453151; 10360571 [H]