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The map label for this gene is rimO [H]

Identifier: 220932034

GI number: 220932034

Start: 1291416

End: 1292744

Strand: Reverse

Name: rimO [H]

Synonym: Hore_11970

Alternate gene names: 220932034

Gene position: 1292744-1291416 (Counterclockwise)

Preceding gene: 220932035

Following gene: 220932033

Centisome position: 50.14

GC content: 33.11

Gene sequence:

>1329_bases
ATGATTAAAGTAGGGCTTATATCACTTGGATGTCCTAAAAATCAAGTTGACAGTGAAATAATGTTAGGTTTTTTATCTAA
TGATAAAAATTTTACTGAAGTTGACGACCTTGAGTTAGCAGATGTTATAGTTATTAATACATGTGGTTTTATTCAGGATG
CAAAAGAAGAATCGATAGAAACGATTCTTGAAGCTGGTCAGTACAAATCTGTAGGGAATTGTAAAGCTTTAATTGTTACC
GGTTGTTTAACACAACGGTATAGAGAAGAAATACTGGAGAATATGCCAGAAGTTGATGCTATTCTGGGAACAGGAAATTT
TGATAAAATAATTCCTACAATTAAAAAGGTCTTAAAAGGTCAAAGGGTTAGTAATATAGGTAATCCGACTTTTAACTATA
ATGATGTTCCCCGGAAAATCAGGACTTCACATTTTGCATATGTTAAAATAGCTGAAGGTTGTGATAACAGGTGTACCTAT
TGCAGTATTCCCTATATACGAGGAAAACTAACAAGTCGACCGATAGAAGATATTTATAATGAGGTTGTAAAACTGGGTCA
GCAGGGGATTAAAGAAATCATTCTTGTAGCCCAGGATACAACTCAGTATGGAAAAGATATTTATGGTAAAAGTATGCTCT
TACCTCTATTAAAAGAACTGGTTGAGATTGATAGTATTAAATGGATCAGGTTATTATACTGTTATCCTGAAAGAATAACC
GATGAGTTGTTGTTTTTTATTGCAGAAAACAATAAAATATGTAACTATCTGGATCTCCCCATTCAGCATTCTTCAAATAA
GATAAGGAGATTAATGAATAGGAAAGGAAGCCGGGAAGAACTTATAAACTTGATAAAAAGAGTAAGGGAACTGGTTCCTG
ATATAGTAATCAGAACATCATTAATTGTAGGTTTTCCCGGAGAAACAGATGAAGACTTTAATGATTTATTTAATTTTGTC
AAAGAAATTAAATTTGATAGACTCGGTGTATTCAAATACTCCCGGGAAGAAAATACTCCTGCAGCTTCATTTCCTGGACA
GATTAGTGAAAAAATTAAAGACGAGAGATATAATAAAATTATGGAGGTCCAGCAGAAAATATCAAGAAATAAAAATCAGG
AAAGAATAGGTGAGAAGGTAAAAGTATTAATAGATGAACTAACAGATGAAATGGCTATAGGAAGGACCCAGTATGATGCC
CCTGAAATTGATAACCAGGTTTATGTCTCCTGTAATGGAAAAATTAGTGAAGGTGACATGATTTTATGCAGGGTTACTGA
GGCTTATGAATATGATTTACTGGGAGAGTGTATTTATGAATTTGCCTAA

Upstream 100 bases:

>100_bases
TCCCGGCAACCCGACCATTTTTTTATAAAACAACTGAGGTGTTAATGCCTCAGTTAATTTTTTTTGTGAATTGTTTTAAA
TATTCAGGAAAGTGGTGAAC

Downstream 100 bases:

>100_bases
TAAATTAACATTGACCCGTATAATACTCGTACCTGTTTTTATGGGTTTTTTATTTCTCCGTAAGGACTACCCCGGTTATG
CTGAAGTACTGGCCTTGATA

Product: MiaB-like tRNA modifying protein

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO [H]

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MIKVGLISLGCPKNQVDSEIMLGFLSNDKNFTEVDDLELADVIVINTCGFIQDAKEESIETILEAGQYKSVGNCKALIVT
GCLTQRYREEILENMPEVDAILGTGNFDKIIPTIKKVLKGQRVSNIGNPTFNYNDVPRKIRTSHFAYVKIAEGCDNRCTY
CSIPYIRGKLTSRPIEDIYNEVVKLGQQGIKEIILVAQDTTQYGKDIYGKSMLLPLLKELVEIDSIKWIRLLYCYPERIT
DELLFFIAENNKICNYLDLPIQHSSNKIRRLMNRKGSREELINLIKRVRELVPDIVIRTSLIVGFPGETDEDFNDLFNFV
KEIKFDRLGVFKYSREENTPAASFPGQISEKIKDERYNKIMEVQQKISRNKNQERIGEKVKVLIDELTDEMAIGRTQYDA
PEIDNQVYVSCNGKISEGDMILCRVTEAYEYDLLGECIYEFA

Sequences:

>Translated_442_residues
MIKVGLISLGCPKNQVDSEIMLGFLSNDKNFTEVDDLELADVIVINTCGFIQDAKEESIETILEAGQYKSVGNCKALIVT
GCLTQRYREEILENMPEVDAILGTGNFDKIIPTIKKVLKGQRVSNIGNPTFNYNDVPRKIRTSHFAYVKIAEGCDNRCTY
CSIPYIRGKLTSRPIEDIYNEVVKLGQQGIKEIILVAQDTTQYGKDIYGKSMLLPLLKELVEIDSIKWIRLLYCYPERIT
DELLFFIAENNKICNYLDLPIQHSSNKIRRLMNRKGSREELINLIKRVRELVPDIVIRTSLIVGFPGETDEDFNDLFNFV
KEIKFDRLGVFKYSREENTPAASFPGQISEKIKDERYNKIMEVQQKISRNKNQERIGEKVKVLIDELTDEMAIGRTQYDA
PEIDNQVYVSCNGKISEGDMILCRVTEAYEYDLLGECIYEFA
>Mature_442_residues
MIKVGLISLGCPKNQVDSEIMLGFLSNDKNFTEVDDLELADVIVINTCGFIQDAKEESIETILEAGQYKSVGNCKALIVT
GCLTQRYREEILENMPEVDAILGTGNFDKIIPTIKKVLKGQRVSNIGNPTFNYNDVPRKIRTSHFAYVKIAEGCDNRCTY
CSIPYIRGKLTSRPIEDIYNEVVKLGQQGIKEIILVAQDTTQYGKDIYGKSMLLPLLKELVEIDSIKWIRLLYCYPERIT
DELLFFIAENNKICNYLDLPIQHSSNKIRRLMNRKGSREELINLIKRVRELVPDIVIRTSLIVGFPGETDEDFNDLFNFV
KEIKFDRLGVFKYSREENTPAASFPGQISEKIKDERYNKIMEVQQKISRNKNQERIGEKVKVLIDELTDEMAIGRTQYDA
PEIDNQVYVSCNGKISEGDMILCRVTEAYEYDLLGECIYEFA

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12 [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=478, Percent_Identity=27.6150627615063, Blast_Score=176, Evalue=5e-44,
Organism=Homo sapiens, GI28872782, Length=478, Percent_Identity=27.6150627615063, Blast_Score=176, Evalue=5e-44,
Organism=Homo sapiens, GI93277076, Length=395, Percent_Identity=28.1012658227848, Blast_Score=159, Evalue=7e-39,
Organism=Escherichia coli, GI1787057, Length=446, Percent_Identity=40.1345291479821, Blast_Score=334, Evalue=6e-93,
Organism=Escherichia coli, GI1786882, Length=449, Percent_Identity=28.9532293986637, Blast_Score=185, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI17553146, Length=430, Percent_Identity=26.7441860465116, Blast_Score=152, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI71996771, Length=326, Percent_Identity=30.3680981595092, Blast_Score=128, Evalue=7e-30,
Organism=Drosophila melanogaster, GI21356207, Length=483, Percent_Identity=26.5010351966874, Blast_Score=178, Evalue=6e-45,
Organism=Drosophila melanogaster, GI19922432, Length=458, Percent_Identity=25.764192139738, Blast_Score=146, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 50623; Mature: 50623

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKVGLISLGCPKNQVDSEIMLGFLSNDKNFTEVDDLELADVIVINTCGFIQDAKEESIE
CEEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHCEEEEECCCCHHHHHHHHHH
TILEAGQYKSVGNCKALIVTGCLTQRYREEILENMPEVDAILGTGNFDKIIPTIKKVLKG
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHEECCCCHHHHHHHHHHHHCC
QRVSNIGNPTFNYNDVPRKIRTSHFAYVKIAEGCDNRCTYCSIPYIRGKLTSRPIEDIYN
CHHHCCCCCCCCCCCCCHHHHHCCEEEEEEECCCCCCCCEEECCHHHCCCCCCCHHHHHH
EVVKLGQQGIKEIILVAQDTTQYGKDIYGKSMLLPLLKELVEIDSIKWIRLLYCYPERIT
HHHHHHHHHHHHHEEEECCHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHEECCCHHHH
DELLFFIAENNKICNYLDLPIQHSSNKIRRLMNRKGSREELINLIKRVRELVPDIVIRTS
HHHEEEEECCCCEECEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
LIVGFPGETDEDFNDLFNFVKEIKFDRLGVFKYSREENTPAASFPGQISEKIKDERYNKI
EEEECCCCCCHHHHHHHHHHHHHCHHHHCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHH
MEVQQKISRNKNQERIGEKVKVLIDELTDEMAIGRTQYDAPEIDNQVYVSCNGKISEGDM
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCEECCCCE
ILCRVTEAYEYDLLGECIYEFA
EEEEECCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIKVGLISLGCPKNQVDSEIMLGFLSNDKNFTEVDDLELADVIVINTCGFIQDAKEESIE
CEEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHCEEEEECCCCHHHHHHHHHH
TILEAGQYKSVGNCKALIVTGCLTQRYREEILENMPEVDAILGTGNFDKIIPTIKKVLKG
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHEECCCCHHHHHHHHHHHHCC
QRVSNIGNPTFNYNDVPRKIRTSHFAYVKIAEGCDNRCTYCSIPYIRGKLTSRPIEDIYN
CHHHCCCCCCCCCCCCCHHHHHCCEEEEEEECCCCCCCCEEECCHHHCCCCCCCHHHHHH
EVVKLGQQGIKEIILVAQDTTQYGKDIYGKSMLLPLLKELVEIDSIKWIRLLYCYPERIT
HHHHHHHHHHHHHEEEECCHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHEECCCHHHH
DELLFFIAENNKICNYLDLPIQHSSNKIRRLMNRKGSREELINLIKRVRELVPDIVIRTS
HHHEEEEECCCCEECEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
LIVGFPGETDEDFNDLFNFVKEIKFDRLGVFKYSREENTPAASFPGQISEKIKDERYNKI
EEEECCCCCCHHHHHHHHHHHHHCHHHHCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHH
MEVQQKISRNKNQERIGEKVKVLIDELTDEMAIGRTQYDAPEIDNQVYVSCNGKISEGDM
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCEECCCCE
ILCRVTEAYEYDLLGECIYEFA
EEEEECCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA