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The map label for this gene is rlmN [H]

Identifier: 220931837

GI number: 220931837

Start: 1084507

End: 1085556

Strand: Direct

Name: rlmN [H]

Synonym: Hore_09940

Alternate gene names: 220931837

Gene position: 1084507-1085556 (Clockwise)

Preceding gene: 220931836

Following gene: 220931838

Centisome position: 42.07

GC content: 38.67

Gene sequence:

>1050_bases
ATGGAGACAAAAGAGGATTTGAAGTCATTAAGTAGAAAAGAGTTATTAATGTGGTTTGAAAAGAGGGGATATCCTTCATT
CAGAGCCAGCCAATTGTTTAACTGGATTTACCGTAATGGGGTTGATGAATTCTCCAGGATGAATAATCTGCCTCTGGTCT
TAAGGGAGGAGCTGGAGGAAAAAAGTTATCTTACTAAATTAAAGATAGTTAATAAAAGCAAAGCAGAAGATGGAACTGTA
AAATACCTCTGGGAACTTAAAGATGGGGAAACTATTGAAAGTGTTTTTATACCCTATGAGGGAAGCCGTAATAGTGTTTG
TATTTCGAGTCAGGTTGGTTGTTCACTGGGGTGTAAATTTTGTGCCACCGGACTAACTGGATTAATCAGGAATTTAACAC
CCGGGGAGATTGTTGATCAGGTTTTACAGATTCAAAAGGAAATTAGTAATGATAAATACGGGTCCCCCAGGGTATCCAAT
GTTGTTTTTATGGGGATGGGGGAACCCCTGGCCAATATGAAATCTGTCCTTAAAGCAATAGAAATCATGAATGATAGTAA
GGGCTTAAATATTGGCAAAAGAAAAATTACAGTGTCGACCTCGGGATTGGTACCCCAGATCAAAGAACTGGCTGATAAAA
AATTGCAGATAGTTCTGGCTATATCTTTAAATGCACCCAATAATGCGCTCAGGGATAAACTAATGCCTATAAACCGGAAA
TTTCCCCTGGAAAAGCTCCTTGAAGCTGTCAGATATTATACAGAGGTTACCAATAGACGGGTTACTTTTGAGTATGTTTT
ATTAAAGGGAACAAATGATTCCCCTGAACATGCTTTTCAGCTTGTAAATTTACTGAGTGATATTCATGGCCACGTTAATT
TAATACCCTTTAATCCTGTTCAGGAAACAGAGTTTAAGAGACCTTCGAAGGAAACTGTTAACAGGTTTAAGGATATACTT
ATTAATAATGGTGTAGAAACCACGGTCAGGCAGGAAAGAGGTACCAGGATCGAGGCTGCCTGTGGTCAGTTACGCCGCCT
AAATTATTAA

Upstream 100 bases:

>100_bases
ATTTAAAATAACCCCGGAGGGTTACCTGGAGTTCTTCCCCCCTGAAAGTAAGACCGAAGGTTTTTATGGCTAAATTACAA
AAATATGGAGGAAATTATTG

Downstream 100 bases:

>100_bases
AGGGGTGGTTTTTTTGCAGTATTCTGTTTTCAGTGATAAGGGAAAGGTCAGGGAAAAGAATGAAGATAATTATCTGATAA
AATTAAACCCCATCCCGATT

Product: radical SAM enzyme, Cfr family

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
METKEDLKSLSRKELLMWFEKRGYPSFRASQLFNWIYRNGVDEFSRMNNLPLVLREELEEKSYLTKLKIVNKSKAEDGTV
KYLWELKDGETIESVFIPYEGSRNSVCISSQVGCSLGCKFCATGLTGLIRNLTPGEIVDQVLQIQKEISNDKYGSPRVSN
VVFMGMGEPLANMKSVLKAIEIMNDSKGLNIGKRKITVSTSGLVPQIKELADKKLQIVLAISLNAPNNALRDKLMPINRK
FPLEKLLEAVRYYTEVTNRRVTFEYVLLKGTNDSPEHAFQLVNLLSDIHGHVNLIPFNPVQETEFKRPSKETVNRFKDIL
INNGVETTVRQERGTRIEAACGQLRRLNY

Sequences:

>Translated_349_residues
METKEDLKSLSRKELLMWFEKRGYPSFRASQLFNWIYRNGVDEFSRMNNLPLVLREELEEKSYLTKLKIVNKSKAEDGTV
KYLWELKDGETIESVFIPYEGSRNSVCISSQVGCSLGCKFCATGLTGLIRNLTPGEIVDQVLQIQKEISNDKYGSPRVSN
VVFMGMGEPLANMKSVLKAIEIMNDSKGLNIGKRKITVSTSGLVPQIKELADKKLQIVLAISLNAPNNALRDKLMPINRK
FPLEKLLEAVRYYTEVTNRRVTFEYVLLKGTNDSPEHAFQLVNLLSDIHGHVNLIPFNPVQETEFKRPSKETVNRFKDIL
INNGVETTVRQERGTRIEAACGQLRRLNY
>Mature_349_residues
METKEDLKSLSRKELLMWFEKRGYPSFRASQLFNWIYRNGVDEFSRMNNLPLVLREELEEKSYLTKLKIVNKSKAEDGTV
KYLWELKDGETIESVFIPYEGSRNSVCISSQVGCSLGCKFCATGLTGLIRNLTPGEIVDQVLQIQKEISNDKYGSPRVSN
VVFMGMGEPLANMKSVLKAIEIMNDSKGLNIGKRKITVSTSGLVPQIKELADKKLQIVLAISLNAPNNALRDKLMPINRK
FPLEKLLEAVRYYTEVTNRRVTFEYVLLKGTNDSPEHAFQLVNLLSDIHGHVNLIPFNPVQETEFKRPSKETVNRFKDIL
INNGVETTVRQERGTRIEAACGQLRRLNY

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=347, Percent_Identity=42.6512968299712, Blast_Score=285, Evalue=3e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 39754; Mature: 39754

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METKEDLKSLSRKELLMWFEKRGYPSFRASQLFNWIYRNGVDEFSRMNNLPLVLREELEE
CCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHH
KSYLTKLKIVNKSKAEDGTVKYLWELKDGETIESVFIPYEGSRNSVCISSQVGCSLGCKF
HHHHHHHHHHCCCCCCCCCEEEEEEECCCCEEEEEEEECCCCCCCEEEECCCCHHHHHHH
CATGLTGLIRNLTPGEIVDQVLQIQKEISNDKYGSPRVSNVVFMGMGEPLANMKSVLKAI
HHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHH
EIMNDSKGLNIGKRKITVSTSGLVPQIKELADKKLQIVLAISLNAPNNALRDKLMPINRK
HHHCCCCCCCCCCEEEEEECCCCCHHHHHHCCCCEEEEEEEEECCCCHHHHHHCCCCCCC
FPLEKLLEAVRYYTEVTNRRVTFEYVLLKGTNDSPEHAFQLVNLLSDIHGHVNLIPFNPV
CCHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEECCCC
QETEFKRPSKETVNRFKDILINNGVETTVRQERGTRIEAACGQLRRLNY
CHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
METKEDLKSLSRKELLMWFEKRGYPSFRASQLFNWIYRNGVDEFSRMNNLPLVLREELEE
CCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHH
KSYLTKLKIVNKSKAEDGTVKYLWELKDGETIESVFIPYEGSRNSVCISSQVGCSLGCKF
HHHHHHHHHHCCCCCCCCCEEEEEEECCCCEEEEEEEECCCCCCCEEEECCCCHHHHHHH
CATGLTGLIRNLTPGEIVDQVLQIQKEISNDKYGSPRVSNVVFMGMGEPLANMKSVLKAI
HHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHH
EIMNDSKGLNIGKRKITVSTSGLVPQIKELADKKLQIVLAISLNAPNNALRDKLMPINRK
HHHCCCCCCCCCCEEEEEECCCCCHHHHHHCCCCEEEEEEEEECCCCHHHHHHCCCCCCC
FPLEKLLEAVRYYTEVTNRRVTFEYVLLKGTNDSPEHAFQLVNLLSDIHGHVNLIPFNPV
CCHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEECCCC
QETEFKRPSKETVNRFKDILINNGVETTVRQERGTRIEAACGQLRRLNY
CHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA