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The map label for this gene is metK [H]

Identifier: 220931829

GI number: 220931829

Start: 1075718

End: 1076926

Strand: Direct

Name: metK [H]

Synonym: Hore_09860

Alternate gene names: 220931829

Gene position: 1075718-1076926 (Clockwise)

Preceding gene: 220931828

Following gene: 220931830

Centisome position: 41.72

GC content: 43.51

Gene sequence:

>1209_bases
ATGGGCAGAAGGTATTTATTTACCTCAGAATCTGTAACAGAAGGTCACCCTGATAAAATCGCTGATCAGATTTCTGATGC
GGTACTTGATGAGATACTGAGTAAAGATTCTCAGGCCAGAGTTGCCTGTGAAACTTTTGTTACAACCGGTCTGGTGCTGG
TATCGGGGGAGATTTCTACTGATTGTTATGTAGATGTTCCCCATATTGCCAGAGAAACTATCAAAAAAATAGGTTATGAC
CGGGCTAAGTATGGATTTGATGGGGATACCTGTTCTGTTTTAACAGCTATTGATGAACAGTCACCCGATATTGCAATAGG
TGTTGATAAGTCCCTGGAAAGCAAAGTAGGGATAGACGAAAATCAGTTGGGTGCCGGGGATCAGGGGTTAATGTTTGGCT
ATGCCTGTAATGAAACCCCCGAACTTATGCCTTTACCTATTATTCTGGCTCATAAGCTGGCCAGAAGGCTGGCTGAAGTT
AGAAAAAAGGGGATTCTACCTTACCTGAGACCTGATGGTAAAACCCAGGTTACAGTAGAATATGAAGACGATCGACCAGT
TAGAATTGATAAAGTACTGGTTTCAGCTCAGCATCATCCGGATATCAGTTCTACCCAGATTAAAGAAGATATTACTAGAC
ATGTTATAAATTATATAATTGACGAAAACCTGAGGGATGATAGGACAGAGATTTTAGTAAATCCAACCGGTCGTTTTGTT
ATCGGTGGTCCTCATGGTGATACTGGTTTAACCGGGAGGAAGATTATTGTAGATACCTATGGTGGTATTGCCAGACATGG
GGGAGGTGCTTTCTCCGGTAAAGACCCGACAAAAGTTGACCGGTCTGCAGCGTATGCAGCCCGGTATGTGGCTAAAAATA
TTGTAGCGGCTGGTTTAGCCGACAGATGTGAGGTTCAACTGGCCTATGCAATCGGGGTGGCCCGGCCGGTATCAATCATG
GTTGATACCTTCGGGACAGGGAAAGTAAGCCATGATATCCTGGTAAAATTAATAAATGAAAATTTTGATTTAAGACCGGC
CAGTATCATAAGGAACCTGGATTTACGCAAACCAATTTATAGTAAGGTCGCTAAATATGGTCACTTCGGTCGCTCTGATC
TGGATTTGACCTGGGAGAGGACCGATAAAAGTGAGGCCCTGAAAGCTGGTGCAGAAACCTTGGCAGGAAGCAAAATACCG
GAGGATTAA

Upstream 100 bases:

>100_bases
TATAATTAATTATAAATTAATACATATTGGTACAAATTTCATTAAATTATCTAATTTTAAATATTAATTATTAAAAATAT
AATTGAAAGTGGTGATATAA

Downstream 100 bases:

>100_bases
TTCCTTCGGTATTTATATTGTTTAAATATGATTAAATTGTGGTTGGAGATGGGGGTGAGCTCTTGTCTAACAATTCAGGT
GGGGTTAGTGTAATTGACAA

Product: Methionine adenosyltransferase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MGRRYLFTSESVTEGHPDKIADQISDAVLDEILSKDSQARVACETFVTTGLVLVSGEISTDCYVDVPHIARETIKKIGYD
RAKYGFDGDTCSVLTAIDEQSPDIAIGVDKSLESKVGIDENQLGAGDQGLMFGYACNETPELMPLPIILAHKLARRLAEV
RKKGILPYLRPDGKTQVTVEYEDDRPVRIDKVLVSAQHHPDISSTQIKEDITRHVINYIIDENLRDDRTEILVNPTGRFV
IGGPHGDTGLTGRKIIVDTYGGIARHGGGAFSGKDPTKVDRSAAYAARYVAKNIVAAGLADRCEVQLAYAIGVARPVSIM
VDTFGTGKVSHDILVKLINENFDLRPASIIRNLDLRKPIYSKVAKYGHFGRSDLDLTWERTDKSEALKAGAETLAGSKIP
ED

Sequences:

>Translated_402_residues
MGRRYLFTSESVTEGHPDKIADQISDAVLDEILSKDSQARVACETFVTTGLVLVSGEISTDCYVDVPHIARETIKKIGYD
RAKYGFDGDTCSVLTAIDEQSPDIAIGVDKSLESKVGIDENQLGAGDQGLMFGYACNETPELMPLPIILAHKLARRLAEV
RKKGILPYLRPDGKTQVTVEYEDDRPVRIDKVLVSAQHHPDISSTQIKEDITRHVINYIIDENLRDDRTEILVNPTGRFV
IGGPHGDTGLTGRKIIVDTYGGIARHGGGAFSGKDPTKVDRSAAYAARYVAKNIVAAGLADRCEVQLAYAIGVARPVSIM
VDTFGTGKVSHDILVKLINENFDLRPASIIRNLDLRKPIYSKVAKYGHFGRSDLDLTWERTDKSEALKAGAETLAGSKIP
ED
>Mature_401_residues
GRRYLFTSESVTEGHPDKIADQISDAVLDEILSKDSQARVACETFVTTGLVLVSGEISTDCYVDVPHIARETIKKIGYDR
AKYGFDGDTCSVLTAIDEQSPDIAIGVDKSLESKVGIDENQLGAGDQGLMFGYACNETPELMPLPIILAHKLARRLAEVR
KKGILPYLRPDGKTQVTVEYEDDRPVRIDKVLVSAQHHPDISSTQIKEDITRHVINYIIDENLRDDRTEILVNPTGRFVI
GGPHGDTGLTGRKIIVDTYGGIARHGGGAFSGKDPTKVDRSAAYAARYVAKNIVAAGLADRCEVQLAYAIGVARPVSIMV
DTFGTGKVSHDILVKLINENFDLRPASIIRNLDLRKPIYSKVAKYGHFGRSDLDLTWERTDKSEALKAGAETLAGSKIPE
D

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=381, Percent_Identity=56.4304461942257, Blast_Score=407, Evalue=1e-114,
Organism=Homo sapiens, GI5174529, Length=379, Percent_Identity=54.8812664907652, Blast_Score=401, Evalue=1e-112,
Organism=Escherichia coli, GI1789311, Length=388, Percent_Identity=61.0824742268041, Blast_Score=484, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI17538498, Length=402, Percent_Identity=54.726368159204, Blast_Score=426, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17538494, Length=382, Percent_Identity=55.4973821989529, Blast_Score=418, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI25145633, Length=402, Percent_Identity=54.228855721393, Blast_Score=414, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17551082, Length=404, Percent_Identity=51.4851485148515, Blast_Score=404, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI32565905, Length=382, Percent_Identity=51.0471204188482, Blast_Score=363, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI32565911, Length=354, Percent_Identity=53.1073446327684, Blast_Score=358, Evalue=4e-99,
Organism=Caenorhabditis elegans, GI25145635, Length=354, Percent_Identity=52.5423728813559, Blast_Score=345, Evalue=2e-95,
Organism=Caenorhabditis elegans, GI32565903, Length=336, Percent_Identity=52.0833333333333, Blast_Score=325, Evalue=3e-89,
Organism=Caenorhabditis elegans, GI32565909, Length=86, Percent_Identity=53.4883720930233, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6320710, Length=389, Percent_Identity=55.2699228791774, Blast_Score=421, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6323209, Length=386, Percent_Identity=54.6632124352332, Blast_Score=409, Evalue=1e-115,
Organism=Drosophila melanogaster, GI45552159, Length=382, Percent_Identity=53.1413612565445, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580529, Length=382, Percent_Identity=53.1413612565445, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580537, Length=382, Percent_Identity=53.1413612565445, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580533, Length=382, Percent_Identity=53.1413612565445, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580535, Length=382, Percent_Identity=53.1413612565445, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580531, Length=382, Percent_Identity=53.1413612565445, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580527, Length=382, Percent_Identity=52.3560209424084, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580525, Length=382, Percent_Identity=52.3560209424084, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580541, Length=366, Percent_Identity=51.3661202185792, Blast_Score=354, Evalue=7e-98,
Organism=Drosophila melanogaster, GI24580539, Length=127, Percent_Identity=51.1811023622047, Blast_Score=131, Evalue=1e-30,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 43964; Mature: 43833

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRRYLFTSESVTEGHPDKIADQISDAVLDEILSKDSQARVACETFVTTGLVLVSGEIST
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCEEEEECCCCC
DCYVDVPHIARETIKKIGYDRAKYGFDGDTCSVLTAIDEQSPDIAIGVDKSLESKVGIDE
CEEEECHHHHHHHHHHCCCCHHCCCCCCCCCCEEEECCCCCCCEEEECCCCHHHHCCCCC
NQLGAGDQGLMFGYACNETPELMPLPIILAHKLARRLAEVRKKGILPYLRPDGKTQVTVE
CCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEEEE
YEDDRPVRIDKVLVSAQHHPDISSTQIKEDITRHVINYIIDENLRDDRTEILVNPTGRFV
ECCCCCEEHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEE
IGGPHGDTGLTGRKIIVDTYGGIARHGGGAFSGKDPTKVDRSAAYAARYVAKNIVAAGLA
EECCCCCCCCCCCEEEEECCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
DRCEVQLAYAIGVARPVSIMVDTFGTGKVSHDILVKLINENFDLRPASIIRNLDLRKPIY
HHHHEEEEEHHCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHCCCCCCHHH
SKVAKYGHFGRSDLDLTWERTDKSEALKAGAETLAGSKIPED
HHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
GRRYLFTSESVTEGHPDKIADQISDAVLDEILSKDSQARVACETFVTTGLVLVSGEIST
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCEEEEECCCCC
DCYVDVPHIARETIKKIGYDRAKYGFDGDTCSVLTAIDEQSPDIAIGVDKSLESKVGIDE
CEEEECHHHHHHHHHHCCCCHHCCCCCCCCCCEEEECCCCCCCEEEECCCCHHHHCCCCC
NQLGAGDQGLMFGYACNETPELMPLPIILAHKLARRLAEVRKKGILPYLRPDGKTQVTVE
CCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEEEE
YEDDRPVRIDKVLVSAQHHPDISSTQIKEDITRHVINYIIDENLRDDRTEILVNPTGRFV
ECCCCCEEHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEE
IGGPHGDTGLTGRKIIVDTYGGIARHGGGAFSGKDPTKVDRSAAYAARYVAKNIVAAGLA
EECCCCCCCCCCCEEEEECCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
DRCEVQLAYAIGVARPVSIMVDTFGTGKVSHDILVKLINENFDLRPASIIRNLDLRKPIY
HHHHEEEEEHHCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHCCCCCCHHH
SKVAKYGHFGRSDLDLTWERTDKSEALKAGAETLAGSKIPED
HHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA