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The map label for this gene is amyB [H]

Identifier: 220931813

GI number: 220931813

Start: 1054625

End: 1055905

Strand: Direct

Name: amyB [H]

Synonym: Hore_09700

Alternate gene names: 220931813

Gene position: 1054625-1055905 (Clockwise)

Preceding gene: 220931811

Following gene: 220931814

Centisome position: 40.91

GC content: 37.08

Gene sequence:

>1281_bases
GTGACTATTAAGACATCCGACTGGCTAAAATCAGCTATTATTTATGAGGTGTTTCCACGAAACCATACACAAGAAGGAAA
TATTCAGGGGATAACCAGGGACCTGGAAAGAATAAGGGAACTGGGGGTTGATATCGTCTGGTTAATGCCTGTATATCCTG
TCGGCAGGAAAGGAAGAAAAGGCAAAGAGGGAAGTCCCTATGCTATAAGGGATTACAGGTCTATTGATCCGGCTCTGGGA
ACATCTGAGGATTTTAAAAAATTGGTAGATAAGGCCCATCGACTCAAATTAAAAGTTATAATTGATGTTGTATTCAATCA
TACAGCTATTGATTCGGTACTGGTTAAAAAGCATCCAGAGTGGTTTTATAAAACACCAGAGGGGGAGATATCAAGAAAAA
TTGATGACTGGTCAGATATCGTTGACCTTGATTTTTCCAGTTCTAAATTAAAAGAGTATTTAATTGATACCCTCCAGTAC
TGGGTAGACCTGGGGGTTGATGGGTTCAGGTGTGATGTAGCAGCATTGGTTCCATTAAGCTTCTGGAAAGAAGCCAGAGA
ACAAATTAAAACCGACAGGGAAATTATATGGCTAGCTGAAAGTGTTGAAAAATCTTTTGTTAAATTTTTGAGGGAGTCAG
GATATGTATGCCATTCAGATCCCGAGCTCCATGAGGTTTTTGACCTGACTTATGATTATGATGGTTTTGAATATCTTAAA
TCTTACTTTAAAGGGCAGGGACAAATACATGATTATATAAATCACCTTTATATTCAGGAAACTTTGTACCCGGAAAATGC
CATCAAGATGCGTTTTCTGGAAAATCATGATAATGTAAGGATTGCTTCTATTATTAAAGATAAAACCCGTTTAAAAAACT
GGACTGCTTTTTATCTTCTTTTACCTGGTGCTTCTTTAATATATTCTGGTCAGGAGTACGGGATCGATAATACACCTGAT
TTATTTAACCAGGATCCTATTGACTGGGAAACTGGTGATCCTGATTTTTATTCATATATGAAAAGACTGGTCAGTATTGC
GAGAAAAATAAAGTCTAACTGTTACCGGTTCAGTATTAAAGAAATCGTTAAGGGGATTATTGAAATAAAATGGCAGGGTC
GTGAGGACTGTTATCTGTCTCTCCTGAATCTTGAAGACAGGTATGGGTATCTTGACTGTAATAGTGTTTATTCCGGGTAT
GATATGTTAAACGACCGGGTATATGAATCAGGGGAAAAAATGAAAATAGAGAAAAATCCCCTTATTTTAAAGCTAAAATA
G

Upstream 100 bases:

>100_bases
TAAGTTGTTATTTTTTTAAAACCAAAGGTATTGACCTGTATTTAGTTAAAAGTAAAAACCACTTATAGTTATTCTAACAT
TTCACAGGGAGGTATTTTTA

Downstream 100 bases:

>100_bases
ATTATAAAGTAGGCAGGATAATGTATAATTATTATATTTAAGGGTTGATTTTTATAATGGAGAGGGGATAATATTAGATG
GGAAAAGATAATTATGATGT

Product: alpha amylase

Products: NA

Alternate protein names: 1,4-alpha-D-glucan glucanohydrolase [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MTIKTSDWLKSAIIYEVFPRNHTQEGNIQGITRDLERIRELGVDIVWLMPVYPVGRKGRKGKEGSPYAIRDYRSIDPALG
TSEDFKKLVDKAHRLKLKVIIDVVFNHTAIDSVLVKKHPEWFYKTPEGEISRKIDDWSDIVDLDFSSSKLKEYLIDTLQY
WVDLGVDGFRCDVAALVPLSFWKEAREQIKTDREIIWLAESVEKSFVKFLRESGYVCHSDPELHEVFDLTYDYDGFEYLK
SYFKGQGQIHDYINHLYIQETLYPENAIKMRFLENHDNVRIASIIKDKTRLKNWTAFYLLLPGASLIYSGQEYGIDNTPD
LFNQDPIDWETGDPDFYSYMKRLVSIARKIKSNCYRFSIKEIVKGIIEIKWQGREDCYLSLLNLEDRYGYLDCNSVYSGY
DMLNDRVYESGEKMKIEKNPLILKLK

Sequences:

>Translated_426_residues
MTIKTSDWLKSAIIYEVFPRNHTQEGNIQGITRDLERIRELGVDIVWLMPVYPVGRKGRKGKEGSPYAIRDYRSIDPALG
TSEDFKKLVDKAHRLKLKVIIDVVFNHTAIDSVLVKKHPEWFYKTPEGEISRKIDDWSDIVDLDFSSSKLKEYLIDTLQY
WVDLGVDGFRCDVAALVPLSFWKEAREQIKTDREIIWLAESVEKSFVKFLRESGYVCHSDPELHEVFDLTYDYDGFEYLK
SYFKGQGQIHDYINHLYIQETLYPENAIKMRFLENHDNVRIASIIKDKTRLKNWTAFYLLLPGASLIYSGQEYGIDNTPD
LFNQDPIDWETGDPDFYSYMKRLVSIARKIKSNCYRFSIKEIVKGIIEIKWQGREDCYLSLLNLEDRYGYLDCNSVYSGY
DMLNDRVYESGEKMKIEKNPLILKLK
>Mature_425_residues
TIKTSDWLKSAIIYEVFPRNHTQEGNIQGITRDLERIRELGVDIVWLMPVYPVGRKGRKGKEGSPYAIRDYRSIDPALGT
SEDFKKLVDKAHRLKLKVIIDVVFNHTAIDSVLVKKHPEWFYKTPEGEISRKIDDWSDIVDLDFSSSKLKEYLIDTLQYW
VDLGVDGFRCDVAALVPLSFWKEAREQIKTDREIIWLAESVEKSFVKFLRESGYVCHSDPELHEVFDLTYDYDGFEYLKS
YFKGQGQIHDYINHLYIQETLYPENAIKMRFLENHDNVRIASIIKDKTRLKNWTAFYLLLPGASLIYSGQEYGIDNTPDL
FNQDPIDWETGDPDFYSYMKRLVSIARKIKSNCYRFSIKEIVKGIIEIKWQGREDCYLSLLNLEDRYGYLDCNSVYSGYD
MLNDRVYESGEKMKIEKNPLILKLK

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=389, Percent_Identity=22.107969151671, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1790687, Length=387, Percent_Identity=24.8062015503876, Blast_Score=97, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6321731, Length=409, Percent_Identity=26.161369193154, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6319776, Length=409, Percent_Identity=26.161369193154, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6321726, Length=401, Percent_Identity=26.1845386533666, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324416, Length=401, Percent_Identity=25.4364089775561, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6322241, Length=401, Percent_Identity=25.4364089775561, Blast_Score=86, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6322021, Length=401, Percent_Identity=25.4364089775561, Blast_Score=86, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6322245, Length=217, Percent_Identity=27.1889400921659, Blast_Score=83, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24586587, Length=404, Percent_Identity=24.7524752475248, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24586591, Length=409, Percent_Identity=22.7383863080685, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24586597, Length=426, Percent_Identity=25.1173708920188, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24586599, Length=218, Percent_Identity=29.3577981651376, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24583745, Length=396, Percent_Identity=23.2323232323232, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24586589, Length=400, Percent_Identity=23.75, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24583749, Length=397, Percent_Identity=23.4256926952141, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24583747, Length=397, Percent_Identity=23.4256926952141, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24586593, Length=388, Percent_Identity=23.9690721649485, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI45549022, Length=425, Percent_Identity=21.6470588235294, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI221330053, Length=397, Percent_Identity=22.9219143576826, Blast_Score=79, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =3.2.1.1 [H]

Molecular weight: Translated: 49995; Mature: 49863

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIKTSDWLKSAIIYEVFPRNHTQEGNIQGITRDLERIRELGVDIVWLMPVYPVGRKGRK
CCCCCHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCC
GKEGSPYAIRDYRSIDPALGTSEDFKKLVDKAHRLKLKVIIDVVFNHTAIDSVLVKKHPE
CCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCC
WFYKTPEGEISRKIDDWSDIVDLDFSSSKLKEYLIDTLQYWVDLGVDGFRCDVAALVPLS
CEEECCCCHHHHCCCCHHHHEECCCCHHHHHHHHHHHHHHHHHCCCCCCEECHHHHHHHH
FWKEAREQIKTDREIIWLAESVEKSFVKFLRESGYVCHSDPELHEVFDLTYDYDGFEYLK
HHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCEECCCCCHHHHHHCCCCCCHHHHHH
SYFKGQGQIHDYINHLYIQETLYPENAIKMRFLENHDNVRIASIIKDKTRLKNWTAFYLL
HHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEHHHHHHHHHHCCCEEEEEE
LPGASLIYSGQEYGIDNTPDLFNQDPIDWETGDPDFYSYMKRLVSIARKIKSNCYRFSIK
ECCCCEEECCHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEHH
EIVKGIIEIKWQGREDCYLSLLNLEDRYGYLDCNSVYSGYDMLNDRVYESGEKMKIEKNP
HHHHHHHEEEECCCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHCCCCEEEEECCC
LILKLK
EEEEEC
>Mature Secondary Structure 
TIKTSDWLKSAIIYEVFPRNHTQEGNIQGITRDLERIRELGVDIVWLMPVYPVGRKGRK
CCCCHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCC
GKEGSPYAIRDYRSIDPALGTSEDFKKLVDKAHRLKLKVIIDVVFNHTAIDSVLVKKHPE
CCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCC
WFYKTPEGEISRKIDDWSDIVDLDFSSSKLKEYLIDTLQYWVDLGVDGFRCDVAALVPLS
CEEECCCCHHHHCCCCHHHHEECCCCHHHHHHHHHHHHHHHHHCCCCCCEECHHHHHHHH
FWKEAREQIKTDREIIWLAESVEKSFVKFLRESGYVCHSDPELHEVFDLTYDYDGFEYLK
HHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCEECCCCCHHHHHHCCCCCCHHHHHH
SYFKGQGQIHDYINHLYIQETLYPENAIKMRFLENHDNVRIASIIKDKTRLKNWTAFYLL
HHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEHHHHHHHHHHCCCEEEEEE
LPGASLIYSGQEYGIDNTPDLFNQDPIDWETGDPDFYSYMKRLVSIARKIKSNCYRFSIK
ECCCCEEECCHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEHH
EIVKGIIEIKWQGREDCYLSLLNLEDRYGYLDCNSVYSGYDMLNDRVYESGEKMKIEKNP
HHHHHHHEEEECCCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHCCCCEEEEECCC
LILKLK
EEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2458257 [H]