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The map label for this gene is murD [H]

Identifier: 220931750

GI number: 220931750

Start: 979905

End: 981275

Strand: Direct

Name: murD [H]

Synonym: Hore_09070

Alternate gene names: 220931750

Gene position: 979905-981275 (Clockwise)

Preceding gene: 220931749

Following gene: 220931751

Centisome position: 38.01

GC content: 37.86

Gene sequence:

>1371_bases
ATGATAGATGTAAGTAGCAAAAAAGTTGCCGTTATAGGTTTAAGTAAAAGAACCGGTGTTTCAGTAGCCAGAACACTTGT
AAACTGCGGGGCAGAGGTCATTGTTTCTGATATAAAAGAGAAAAAAGACCTTAAGGACGAAATTGAAATGTTAAATGAAT
ATGAGGTTGAATTTGATCTGGGTGGTCATTCAAATAAATTACTGGATGTTGATTTAATAATAGTCAGTCCCGGTGTCCCC
CTTAATCTGCCATTTTTTAAAAGTATTTCTGAAAAAGGGATACCTGTTATCAGTGAAATAGAGTTTGCCTATCATCTTAC
TGAAGCAAACATTATTGCCATTACCGGAACAAATGGCAAGACGACTACTACTGCTTTAACTGGTGAGATATTGGATAAAG
CAGATATTGGAGAAGTCAGGGTTGGTGGTAATATTGGAAAGCCATTAATTGATGTGGTTCAGGGACTGAAGAAAAATGAC
TGGGTGGTGGCAGAAGTTAGCTCATTTCAACTGGAAACAATCAGGGATTTTAAACCCCATATCAGTGCCTTTTTAAATTT
TTCCCCTGATCATCTGGATAGGCACTTGAGTGTGGAGAATTACTGGAAAGCTAAAAAAAGGATTTTTGAAAACCAAATGT
CCGGTGATTATGCCCTTGTCAATTATGATGATTATAAGGTACTAAAAGCAGTTGAAGATTGTTCAGCGAGAGTTTTTGGT
GTTTCTTTAAAAGACAATATAGAACAGGGTATATACCTTGAAGAAGATAAATTAATTATTAATTCAGCAGATAGAAGTGA
AGTTGTCTTACCTGTTGAGAGCATTCCTTTAAAGGGTAAACACAATATATATAATATAGCCTTTGCTTCCCTGATAGCTT
ACCTGGCAGGGGCTAGTACTGAAGCTATAAAAGAAGGGGTTTATCAGTTTATACCTGAACCTCATAGACTTGAAGAGGTT
ACAGTGCTGAAAGATAGGGTTATGGTTATAGATGATTCCAAGGCAACAAATCCCCATGCGGCTATTAATGCCCTTAAGTC
CTTTGATCAACCTATTGTTTTGATAGCAGGTGGTCAGGATAGAGATGCTGATTTTTCTGAATTTGCCAGGGAAATTGTGT
CCCGGGTCAGGGCTCTGATTCTGTTAGGGGAGACAAAAGATAAACTGGCAGAAACTGTATTAAGGATGGGATTTAATAAT
ATATATAAAGTAAGGGATATGAATGAAGCAGTGAGAAAAGCAATTGCCCATACCGAACCAGGTGATGTATTATTATTATC
CCCCGGATGCCCCAGCTGGGATATGTATGACAGTTATAAAAAAAGGGGCCAGGAGTTCAAGGAGGCAGTTAATAAGATAA
GGGGGAGTTAG

Upstream 100 bases:

>100_bases
CGGAATTATATCTTTTTATCTTATCGGTAGTTAACTTAAGGTTTTTTATAATTGATTTTATCCTTGCAAAAATGTTTGAG
TAAAAAGGAGTAGACTTTCC

Downstream 100 bases:

>100_bases
TTGTGGAGGACCAGAAAACCCCGGATCTGGTACTGTTTTTTGTTGTGGTTACTTTGCTGGTGATTGGTTTAATTATGATC
CTCAGTGCCAGTTCTATAAG

Product: UDP-N-acetylmuramoylalanine--D-glutamate ligase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MIDVSSKKVAVIGLSKRTGVSVARTLVNCGAEVIVSDIKEKKDLKDEIEMLNEYEVEFDLGGHSNKLLDVDLIIVSPGVP
LNLPFFKSISEKGIPVISEIEFAYHLTEANIIAITGTNGKTTTTALTGEILDKADIGEVRVGGNIGKPLIDVVQGLKKND
WVVAEVSSFQLETIRDFKPHISAFLNFSPDHLDRHLSVENYWKAKKRIFENQMSGDYALVNYDDYKVLKAVEDCSARVFG
VSLKDNIEQGIYLEEDKLIINSADRSEVVLPVESIPLKGKHNIYNIAFASLIAYLAGASTEAIKEGVYQFIPEPHRLEEV
TVLKDRVMVIDDSKATNPHAAINALKSFDQPIVLIAGGQDRDADFSEFAREIVSRVRALILLGETKDKLAETVLRMGFNN
IYKVRDMNEAVRKAIAHTEPGDVLLLSPGCPSWDMYDSYKKRGQEFKEAVNKIRGS

Sequences:

>Translated_456_residues
MIDVSSKKVAVIGLSKRTGVSVARTLVNCGAEVIVSDIKEKKDLKDEIEMLNEYEVEFDLGGHSNKLLDVDLIIVSPGVP
LNLPFFKSISEKGIPVISEIEFAYHLTEANIIAITGTNGKTTTTALTGEILDKADIGEVRVGGNIGKPLIDVVQGLKKND
WVVAEVSSFQLETIRDFKPHISAFLNFSPDHLDRHLSVENYWKAKKRIFENQMSGDYALVNYDDYKVLKAVEDCSARVFG
VSLKDNIEQGIYLEEDKLIINSADRSEVVLPVESIPLKGKHNIYNIAFASLIAYLAGASTEAIKEGVYQFIPEPHRLEEV
TVLKDRVMVIDDSKATNPHAAINALKSFDQPIVLIAGGQDRDADFSEFAREIVSRVRALILLGETKDKLAETVLRMGFNN
IYKVRDMNEAVRKAIAHTEPGDVLLLSPGCPSWDMYDSYKKRGQEFKEAVNKIRGS
>Mature_456_residues
MIDVSSKKVAVIGLSKRTGVSVARTLVNCGAEVIVSDIKEKKDLKDEIEMLNEYEVEFDLGGHSNKLLDVDLIIVSPGVP
LNLPFFKSISEKGIPVISEIEFAYHLTEANIIAITGTNGKTTTTALTGEILDKADIGEVRVGGNIGKPLIDVVQGLKKND
WVVAEVSSFQLETIRDFKPHISAFLNFSPDHLDRHLSVENYWKAKKRIFENQMSGDYALVNYDDYKVLKAVEDCSARVFG
VSLKDNIEQGIYLEEDKLIINSADRSEVVLPVESIPLKGKHNIYNIAFASLIAYLAGASTEAIKEGVYQFIPEPHRLEEV
TVLKDRVMVIDDSKATNPHAAINALKSFDQPIVLIAGGQDRDADFSEFAREIVSRVRALILLGETKDKLAETVLRMGFNN
IYKVRDMNEAVRKAIAHTEPGDVLLLSPGCPSWDMYDSYKKRGQEFKEAVNKIRGS

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=457, Percent_Identity=33.9168490153173, Blast_Score=184, Evalue=9e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 50614; Mature: 50614

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDVSSKKVAVIGLSKRTGVSVARTLVNCGAEVIVSDIKEKKDLKDEIEMLNEYEVEFDL
CCCCCCCEEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEE
GGHSNKLLDVDLIIVSPGVPLNLPFFKSISEKGIPVISEIEFAYHLTEANIIAITGTNGK
CCCCCCEEEEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEHEEEEEECCEEEEEECCCCC
TTTTALTGEILDKADIGEVRVGGNIGKPLIDVVQGLKKNDWVVAEVSSFQLETIRDFKPH
EEEEEEEHHHHCCCCCCCEEECCCCCHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHH
ISAFLNFSPDHLDRHLSVENYWKAKKRIFENQMSGDYALVNYDDYKVLKAVEDCSARVFG
HHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCEEEE
VSLKDNIEQGIYLEEDKLIINSADRSEVVLPVESIPLKGKHNIYNIAFASLIAYLAGAST
EEHHHCCCCCEEEECCEEEEECCCCCEEEEEHHCCCCCCCCCCHHHHHHHHHHHHHCCCH
EAIKEGVYQFIPEPHRLEEVTVLKDRVMVIDDSKATNPHAAINALKSFDQPIVLIAGGQD
HHHHHHHHHHCCCCCHHHHHEEEECEEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCC
RDADFSEFAREIVSRVRALILLGETKDKLAETVLRMGFNNIYKVRDMNEAVRKAIAHTEP
CCCCHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCCC
GDVLLLSPGCPSWDMYDSYKKRGQEFKEAVNKIRGS
CCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIDVSSKKVAVIGLSKRTGVSVARTLVNCGAEVIVSDIKEKKDLKDEIEMLNEYEVEFDL
CCCCCCCEEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEE
GGHSNKLLDVDLIIVSPGVPLNLPFFKSISEKGIPVISEIEFAYHLTEANIIAITGTNGK
CCCCCCEEEEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEHEEEEEECCEEEEEECCCCC
TTTTALTGEILDKADIGEVRVGGNIGKPLIDVVQGLKKNDWVVAEVSSFQLETIRDFKPH
EEEEEEEHHHHCCCCCCCEEECCCCCHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHH
ISAFLNFSPDHLDRHLSVENYWKAKKRIFENQMSGDYALVNYDDYKVLKAVEDCSARVFG
HHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCEEEE
VSLKDNIEQGIYLEEDKLIINSADRSEVVLPVESIPLKGKHNIYNIAFASLIAYLAGAST
EEHHHCCCCCEEEECCEEEEECCCCCEEEEEHHCCCCCCCCCCHHHHHHHHHHHHHCCCH
EAIKEGVYQFIPEPHRLEEVTVLKDRVMVIDDSKATNPHAAINALKSFDQPIVLIAGGQD
HHHHHHHHHHCCCCCHHHHHEEEECEEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCC
RDADFSEFAREIVSRVRALILLGETKDKLAETVLRMGFNNIYKVRDMNEAVRKAIAHTEP
CCCCHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCCC
GDVLLLSPGCPSWDMYDSYKKRGQEFKEAVNKIRGS
CCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA