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The map label for this gene is ydaP [H]

Identifier: 220931666

GI number: 220931666

Start: 886356

End: 887960

Strand: Reverse

Name: ydaP [H]

Synonym: Hore_08220

Alternate gene names: 220931666

Gene position: 887960-886356 (Counterclockwise)

Preceding gene: 220931670

Following gene: 220931665

Centisome position: 34.44

GC content: 39.19

Gene sequence:

>1605_bases
ATGGGTCAGAAAGTATCAGAAGTTATTATTGAACAACTCCATAACTTTGGTGTAAAGCATATATATGGGTACGCCGGTGA
TACCATCTTGAAATTTTTTTCAAAGCTAAAGGGGTCACCGGTCAAGCTCTTCACAACTAAACATGAATCTACCGCAGGTT
TTATGGCCTCAGCTGAAGCAAAATTAACCGGAAATCTGGGTGTCTGTATTGCCCACAGTGGTCCTGGCACTACCAATATT
ATTAATGGTATTGCTGATGCGTACAGTGATCGAGTCCCACTCCTTTTAATAACTGGACAGGTTCCCACTTATAATATTGG
AACCGACTATAAGCAATATGTGGATCAGTTAAAACTCACTGAGCCCCTGACCGTTTATTCCAGTATCTTAATAAATCCGG
CCACAGTTATCGATATTTTCTACAAAGCCATGACAATGTCAATTTTAAAAGGAGGAGTAAGTCACATTGTTATTCCTATG
GATCTGTGGGATCAGGAAACAAATGCTATCCCCAGGCCTTATCCTGAACATTTAAATCATAAACAAACAGTTGACGAAAA
CCAGCTTGATAGAGCTGCTACCAGTATAAATAACACTGAAAAAATTTCTATTCTTTATGGTAGAGGTGCTAAAAACTGCC
GTCAAGAATTAATAGAGCTGGCCCAGAAAATTAATGCACCGTTGATTAACACCCTTCCTGTAAGTGGGTTGATCGAATTT
GGTCATCCCCTTAGACTCGGCTGTCTCGGACATGCGGGAAGCAATGAAGCAGCCCGGGCCCTCAATGAAGCAGATTTAAT
ATTAACTGTGGGAGCTACCTGGTGGCCTAAAGATTATACCCCCAGAAAACCCCGGCTAATTCAACTTGACCTGATAAAAG
AAAATATCGGGGCTACACATCCAGTTGATATTGGTCTTATAGGAGATATCAAAAACACCTTACAGACACTTTTATCGAAA
ATAAATTCAAAAAATGAAACAGGTTGGTTAGACCATATAAAGGATTTAAGAAATAACTGGATGGATAGAATCACCAAAGA
ATCACACCAGGAAAACTGGCCTCTTCCGCCCCAGACTATAATAAGAATAATTAGTGAAAATACTCAACCTGATGAAATAA
TTTGCCTTGATTCTGGAGATAATGTTATATGGTTTGGTCGGTATTTTGGTAACAAATGTGCCCATACACTGATTTCGGGG
ACATGGCGAACTATGGGGTTCAGCCTCCCTTCTGCCCTGGCAGCCAGAATTAATAAACCAAATAATCAGGTAACCTCAAT
TATAGGTGATGGAGGTCTATCTATGGTTCTGGCTGAAATCTCGACAGCAGTTAGATATAATTTACCCATAAAAATAATTG
TTTTAAATAATAACTCTCTGGCAATGGAAAAAAACAGAATGGAGGCCGCCGGGTTAGTACCTGAAGAAGTCGAACTTACA
AACCCTGATTTTGTTAAAATAGCTAAAGCCTGTGGATTTGAAGGAAAACGTGCCAATAATATTAATGAACTTCAGGCTCT
TTTAAAGGAAAATAAACAGACAAATGGTCTCTTAATTGATATTCCAACAGCCAGCTATCCCCCATCAGGAACTAAATTAT
CTTGA

Upstream 100 bases:

>100_bases
TATTTTAATAAAAGATTCCTTAAAACTACCCGGTATTAATTTAAGTTCTTTGGATTATAATAAAAACAGTAAATAATTAT
ACTAATGGAGGGAATAGTAT

Downstream 100 bases:

>100_bases
ATATAGACCAATATATAAAATCATGTATATTTTTTAGTAATGTTACCTTGATTTTAAAAAACCTATATGGTATATTTATT
TATAAATTAATTAAATATAT

Product: pyruvate oxidase

Products: acetyl phosphate; CO2; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 534; Mature: 533

Protein sequence:

>534_residues
MGQKVSEVIIEQLHNFGVKHIYGYAGDTILKFFSKLKGSPVKLFTTKHESTAGFMASAEAKLTGNLGVCIAHSGPGTTNI
INGIADAYSDRVPLLLITGQVPTYNIGTDYKQYVDQLKLTEPLTVYSSILINPATVIDIFYKAMTMSILKGGVSHIVIPM
DLWDQETNAIPRPYPEHLNHKQTVDENQLDRAATSINNTEKISILYGRGAKNCRQELIELAQKINAPLINTLPVSGLIEF
GHPLRLGCLGHAGSNEAARALNEADLILTVGATWWPKDYTPRKPRLIQLDLIKENIGATHPVDIGLIGDIKNTLQTLLSK
INSKNETGWLDHIKDLRNNWMDRITKESHQENWPLPPQTIIRIISENTQPDEIICLDSGDNVIWFGRYFGNKCAHTLISG
TWRTMGFSLPSALAARINKPNNQVTSIIGDGGLSMVLAEISTAVRYNLPIKIIVLNNNSLAMEKNRMEAAGLVPEEVELT
NPDFVKIAKACGFEGKRANNINELQALLKENKQTNGLLIDIPTASYPPSGTKLS

Sequences:

>Translated_534_residues
MGQKVSEVIIEQLHNFGVKHIYGYAGDTILKFFSKLKGSPVKLFTTKHESTAGFMASAEAKLTGNLGVCIAHSGPGTTNI
INGIADAYSDRVPLLLITGQVPTYNIGTDYKQYVDQLKLTEPLTVYSSILINPATVIDIFYKAMTMSILKGGVSHIVIPM
DLWDQETNAIPRPYPEHLNHKQTVDENQLDRAATSINNTEKISILYGRGAKNCRQELIELAQKINAPLINTLPVSGLIEF
GHPLRLGCLGHAGSNEAARALNEADLILTVGATWWPKDYTPRKPRLIQLDLIKENIGATHPVDIGLIGDIKNTLQTLLSK
INSKNETGWLDHIKDLRNNWMDRITKESHQENWPLPPQTIIRIISENTQPDEIICLDSGDNVIWFGRYFGNKCAHTLISG
TWRTMGFSLPSALAARINKPNNQVTSIIGDGGLSMVLAEISTAVRYNLPIKIIVLNNNSLAMEKNRMEAAGLVPEEVELT
NPDFVKIAKACGFEGKRANNINELQALLKENKQTNGLLIDIPTASYPPSGTKLS
>Mature_533_residues
GQKVSEVIIEQLHNFGVKHIYGYAGDTILKFFSKLKGSPVKLFTTKHESTAGFMASAEAKLTGNLGVCIAHSGPGTTNII
NGIADAYSDRVPLLLITGQVPTYNIGTDYKQYVDQLKLTEPLTVYSSILINPATVIDIFYKAMTMSILKGGVSHIVIPMD
LWDQETNAIPRPYPEHLNHKQTVDENQLDRAATSINNTEKISILYGRGAKNCRQELIELAQKINAPLINTLPVSGLIEFG
HPLRLGCLGHAGSNEAARALNEADLILTVGATWWPKDYTPRKPRLIQLDLIKENIGATHPVDIGLIGDIKNTLQTLLSKI
NSKNETGWLDHIKDLRNNWMDRITKESHQENWPLPPQTIIRIISENTQPDEIICLDSGDNVIWFGRYFGNKCAHTLISGT
WRTMGFSLPSALAARINKPNNQVTSIIGDGGLSMVLAEISTAVRYNLPIKIIVLNNNSLAMEKNRMEAAGLVPEEVELTN
PDFVKIAKACGFEGKRANNINELQALLKENKQTNGLLIDIPTASYPPSGTKLS

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=543, Percent_Identity=24.8618784530387, Blast_Score=139, Evalue=8e-33,
Organism=Escherichia coli, GI1787096, Length=538, Percent_Identity=30.6691449814126, Blast_Score=257, Evalue=1e-69,
Organism=Escherichia coli, GI87081685, Length=528, Percent_Identity=29.3560606060606, Blast_Score=225, Evalue=6e-60,
Organism=Escherichia coli, GI1790104, Length=544, Percent_Identity=29.5955882352941, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1786717, Length=527, Percent_Identity=23.1499051233397, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI1788716, Length=487, Percent_Identity=23.2032854209446, Blast_Score=103, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17531299, Length=542, Percent_Identity=22.6937269372694, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17531301, Length=542, Percent_Identity=22.6937269372694, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17542570, Length=561, Percent_Identity=23.5294117647059, Blast_Score=89, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6323755, Length=560, Percent_Identity=28.3928571428571, Blast_Score=190, Evalue=6e-49,
Organism=Saccharomyces cerevisiae, GI6320816, Length=540, Percent_Identity=23.5185185185185, Blast_Score=110, Evalue=7e-25,
Organism=Drosophila melanogaster, GI19922626, Length=560, Percent_Identity=25.1785714285714, Blast_Score=144, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 1.2.3.3

Molecular weight: Translated: 58828; Mature: 58697

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQKVSEVIIEQLHNFGVKHIYGYAGDTILKFFSKLKGSPVKLFTTKHESTAGFMASAEA
CCCHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCCCHHEECCE
KLTGNLGVCIAHSGPGTTNIINGIADAYSDRVPLLLITGQVPTYNIGTDYKQYVDQLKLT
EEECCEEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEECCCCCEECCCCHHHHHHHHCCC
EPLTVYSSILINPATVIDIFYKAMTMSILKGGVSHIVIPMDLWDQETNAIPRPYPEHLNH
CHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHCCCCCCCCCCCCHHHCCC
KQTVDENQLDRAATSINNTEKISILYGRGAKNCRQELIELAQKINAPLINTLPVSGLIEF
CCCCCHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCEECCCCHHHHHC
GHPLRLGCLGHAGSNEAARALNEADLILTVGATWWPKDYTPRKPRLIQLDLIKENIGATH
CCCEEEEEECCCCCCHHHHHCCCCCEEEEECCCCCCCCCCCCCCEEEEEEEEHHHCCCCC
PVDIGLIGDIKNTLQTLLSKINSKNETGWLDHIKDLRNNWMDRITKESHQENWPLPPQTI
CEEEEEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
IRIISENTQPDEIICLDSGDNVIWFGRYFGNKCAHTLISGTWRTMGFSLPSALAARINKP
HHHHHCCCCCCEEEEEECCCCEEEEHHHHCCHHHHHHHHCCHHHHCCCCHHHHHHHCCCC
NNQVTSIIGDGGLSMVLAEISTAVRYNLPIKIIVLNNNSLAMEKNRMEAAGLVPEEVELT
CCHHHEEECCCCHHHHHHHHHHHHEECCCEEEEEECCCCEEEECCCHHHHCCCCCCCCCC
NPDFVKIAKACGFEGKRANNINELQALLKENKQTNGLLIDIPTASYPPSGTKLS
CCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
GQKVSEVIIEQLHNFGVKHIYGYAGDTILKFFSKLKGSPVKLFTTKHESTAGFMASAEA
CCHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCCCHHEECCE
KLTGNLGVCIAHSGPGTTNIINGIADAYSDRVPLLLITGQVPTYNIGTDYKQYVDQLKLT
EEECCEEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEECCCCCEECCCCHHHHHHHHCCC
EPLTVYSSILINPATVIDIFYKAMTMSILKGGVSHIVIPMDLWDQETNAIPRPYPEHLNH
CHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHCCCCCCCCCCCCHHHCCC
KQTVDENQLDRAATSINNTEKISILYGRGAKNCRQELIELAQKINAPLINTLPVSGLIEF
CCCCCHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCEECCCCHHHHHC
GHPLRLGCLGHAGSNEAARALNEADLILTVGATWWPKDYTPRKPRLIQLDLIKENIGATH
CCCEEEEEECCCCCCHHHHHCCCCCEEEEECCCCCCCCCCCCCCEEEEEEEEHHHCCCCC
PVDIGLIGDIKNTLQTLLSKINSKNETGWLDHIKDLRNNWMDRITKESHQENWPLPPQTI
CEEEEEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
IRIISENTQPDEIICLDSGDNVIWFGRYFGNKCAHTLISGTWRTMGFSLPSALAARINKP
HHHHHCCCCCCEEEEEECCCCEEEEHHHHCCHHHHHHHHCCHHHHCCCCHHHHHHHCCCC
NNQVTSIIGDGGLSMVLAEISTAVRYNLPIKIIVLNNNSLAMEKNRMEAAGLVPEEVELT
CCHHHEEECCCCHHHHHHHHHHHHEECCCEEEEEECCCCEEEECCCHHHHCCCCCCCCCC
NPDFVKIAKACGFEGKRANNINELQALLKENKQTNGLLIDIPTASYPPSGTKLS
CCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; phosphate; O2; H2O

Specific reaction: pyruvate + phosphate + O2 + H2O = acetyl phosphate + CO2 + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]