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The map label for this gene is mdeA [H]

Identifier: 220931644

GI number: 220931644

Start: 859274

End: 860452

Strand: Direct

Name: mdeA [H]

Synonym: Hore_08000

Alternate gene names: 220931644

Gene position: 859274-860452 (Clockwise)

Preceding gene: 220931643

Following gene: 220931645

Centisome position: 33.33

GC content: 36.9

Gene sequence:

>1179_bases
ATGGATGACTATAAATTTAACACACTATCAATTCATGGTGGAGATGAAGGGAAAAATCCAGAAAATGCTTTGAATTATCC
GCTCTTTTTAACATCAACCTTTACTTTTAATGACCTTGAACATGCTGAAAAGACCTTTTCATTTGAATGTAAAGATTATG
TTTATACAAGAGGGAATAACCCTACAACCAGGTTATTAGAGAAAAGGCTGGCCTGCCTTGAAGGTGGGGCAGATTGTGTT
GTTTTTTCTTCTGGTATGGCAGCTATATCATCAGCACTTCTGTCATTATTAAAGCCGGGTGATGAATTGATTGCCCATCA
GGTTTTGTATGGCTCGTCATATAAGGTAATAAAAGAAGTGTTACCCCGGTACGGTATCAAAACAAGTTTTATTGATTTAA
CTCAAATTGACAAATTAAAAACAGGTATAACTGATAAGACTACATTAATTTATTTTGAAACTCCGGTCAACCCCAATCTT
GAAATAATAGATATTAAAGAAGTTAGCCAGCTATGCTCTTCACATAATATAAAAGTGGTGGTTGATAATACTTTTGCTAC
CCCATTTTACCAGAATCCATTAAAATTGGGAGCAGATGTTGTAATCCACAGTGCTACTAAATACCTGAATGGCCATGGTG
ATGTTCTGGCCGGGGCTGTTATTGCCAGGGATCAGGAATATATACATGAATTAAAATTTAATTATTTAACTGAACTGGGT
GGTGTTTTAAGTCCCTTTAATTCCTGGTTAGTATTAAGAGGTCTTAAAACATTGGGTCTCAGAATGAGAGAACATGAAAA
AAATGCCAGCAAGATTGCTAAGTACCTTAAAAATCATCCGGGGATAAACCGGGTATACTACCCCGGTTTACAGGACTTTA
AGGGTTATGAAATAGCCAAAAAACAAATGTCCGGTTTTGGGGCAATTATAAGTTTTGAAATTATGGGTGGGTATCAGGAT
ATAAAAAGGGCAGTTGAATCCCTGAATTTAATTAAACTGGCCGTTAGCCTCGGAGATGCTGAAACCCTTATTGAATATCC
CTTTGGCATGACTCACCGGGATTATTCCGGTCAAGAACTGGCCAGAATGGGTCTCAATAAAAATATGGTAAGATTGTCGG
TGGGACTTGAAAATTACGAAGATATAATAGCTGACCTGGATCAGGCCTTATCTAAATAA

Upstream 100 bases:

>100_bases
TTGCAAACGGAGTGAACAGAGGTTTTTTAGTTTTACAGGATAAAATGGATAATATATTATTTAAAATAATTAAAAAGTAA
CAGAAGGGAGACAGGGATAG

Downstream 100 bases:

>100_bases
ATATCCATCAGGAACAAAATACTCTTGTTTTTTATGCAAAATAGTGATAGAATATACGATAAGAATTTTTTTACAGGATA
AGGTGGATAGCAATGTTTAC

Product: Cystathionine gamma-synthase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MDDYKFNTLSIHGGDEGKNPENALNYPLFLTSTFTFNDLEHAEKTFSFECKDYVYTRGNNPTTRLLEKRLACLEGGADCV
VFSSGMAAISSALLSLLKPGDELIAHQVLYGSSYKVIKEVLPRYGIKTSFIDLTQIDKLKTGITDKTTLIYFETPVNPNL
EIIDIKEVSQLCSSHNIKVVVDNTFATPFYQNPLKLGADVVIHSATKYLNGHGDVLAGAVIARDQEYIHELKFNYLTELG
GVLSPFNSWLVLRGLKTLGLRMREHEKNASKIAKYLKNHPGINRVYYPGLQDFKGYEIAKKQMSGFGAIISFEIMGGYQD
IKRAVESLNLIKLAVSLGDAETLIEYPFGMTHRDYSGQELARMGLNKNMVRLSVGLENYEDIIADLDQALSK

Sequences:

>Translated_392_residues
MDDYKFNTLSIHGGDEGKNPENALNYPLFLTSTFTFNDLEHAEKTFSFECKDYVYTRGNNPTTRLLEKRLACLEGGADCV
VFSSGMAAISSALLSLLKPGDELIAHQVLYGSSYKVIKEVLPRYGIKTSFIDLTQIDKLKTGITDKTTLIYFETPVNPNL
EIIDIKEVSQLCSSHNIKVVVDNTFATPFYQNPLKLGADVVIHSATKYLNGHGDVLAGAVIARDQEYIHELKFNYLTELG
GVLSPFNSWLVLRGLKTLGLRMREHEKNASKIAKYLKNHPGINRVYYPGLQDFKGYEIAKKQMSGFGAIISFEIMGGYQD
IKRAVESLNLIKLAVSLGDAETLIEYPFGMTHRDYSGQELARMGLNKNMVRLSVGLENYEDIIADLDQALSK
>Mature_392_residues
MDDYKFNTLSIHGGDEGKNPENALNYPLFLTSTFTFNDLEHAEKTFSFECKDYVYTRGNNPTTRLLEKRLACLEGGADCV
VFSSGMAAISSALLSLLKPGDELIAHQVLYGSSYKVIKEVLPRYGIKTSFIDLTQIDKLKTGITDKTTLIYFETPVNPNL
EIIDIKEVSQLCSSHNIKVVVDNTFATPFYQNPLKLGADVVIHSATKYLNGHGDVLAGAVIARDQEYIHELKFNYLTELG
GVLSPFNSWLVLRGLKTLGLRMREHEKNASKIAKYLKNHPGINRVYYPGLQDFKGYEIAKKQMSGFGAIISFEIMGGYQD
IKRAVESLNLIKLAVSLGDAETLIEYPFGMTHRDYSGQELARMGLNKNMVRLSVGLENYEDIIADLDQALSK

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=387, Percent_Identity=38.5012919896641, Blast_Score=255, Evalue=5e-68,
Organism=Homo sapiens, GI299473759, Length=387, Percent_Identity=35.1421188630491, Blast_Score=217, Evalue=1e-56,
Organism=Homo sapiens, GI25453487, Length=386, Percent_Identity=33.6787564766839, Blast_Score=192, Evalue=3e-49,
Organism=Escherichia coli, GI1790375, Length=383, Percent_Identity=38.6422976501305, Blast_Score=279, Evalue=2e-76,
Organism=Escherichia coli, GI1789383, Length=402, Percent_Identity=28.1094527363184, Blast_Score=174, Evalue=8e-45,
Organism=Caenorhabditis elegans, GI17538055, Length=389, Percent_Identity=41.6452442159383, Blast_Score=279, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI17560056, Length=389, Percent_Identity=40.6169665809769, Blast_Score=274, Evalue=6e-74,
Organism=Caenorhabditis elegans, GI71980708, Length=370, Percent_Identity=37.2972972972973, Blast_Score=252, Evalue=2e-67,
Organism=Saccharomyces cerevisiae, GI6319307, Length=396, Percent_Identity=36.1111111111111, Blast_Score=237, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6323333, Length=437, Percent_Identity=33.1807780320366, Blast_Score=224, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6321254, Length=410, Percent_Identity=31.219512195122, Blast_Score=180, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6321170, Length=328, Percent_Identity=28.9634146341463, Blast_Score=155, Evalue=8e-39,
Organism=Saccharomyces cerevisiae, GI6321904, Length=402, Percent_Identity=28.6069651741293, Blast_Score=142, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6322590, Length=348, Percent_Identity=26.4367816091954, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19922554, Length=390, Percent_Identity=38.7179487179487, Blast_Score=268, Evalue=7e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 43796; Mature: 43796

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDYKFNTLSIHGGDEGKNPENALNYPLFLTSTFTFNDLEHAEKTFSFECKDYVYTRGNN
CCCCEEEEEEEECCCCCCCCHHHCCCCEEEEEEEECCHHHHHHHHCCEEECEEEEECCCC
PTTRLLEKRLACLEGGADCVVFSSGMAAISSALLSLLKPGDELIAHQVLYGSSYKVIKEV
HHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHH
LPRYGIKTSFIDLTQIDKLKTGITDKTTLIYFETPVNPNLEIIDIKEVSQLCSSHNIKVV
HHHCCCCCCEECHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEHHHHHHHHCCCCEEEE
VDNTFATPFYQNPLKLGADVVIHSATKYLNGHGDVLAGAVIARDQEYIHELKFNYLTELG
EECCCCCCCCCCHHHHCCHHEEEHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHH
GVLSPFNSWLVLRGLKTLGLRMREHEKNASKIAKYLKNHPGINRVYYPGLQDFKGYEIAK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHH
KQMSGFGAIISFEIMGGYQDIKRAVESLNLIKLAVSLGDAETLIEYPFGMTHRDYSGQEL
HHHCCCCEEEEEEEECCHHHHHHHHHHHHHHHEEECCCCHHHHHHCCCCCCCCCCCHHHH
ARMGLNKNMVRLSVGLENYEDIIADLDQALSK
HHHCCCCCEEEEEECCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDDYKFNTLSIHGGDEGKNPENALNYPLFLTSTFTFNDLEHAEKTFSFECKDYVYTRGNN
CCCCEEEEEEEECCCCCCCCHHHCCCCEEEEEEEECCHHHHHHHHCCEEECEEEEECCCC
PTTRLLEKRLACLEGGADCVVFSSGMAAISSALLSLLKPGDELIAHQVLYGSSYKVIKEV
HHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHH
LPRYGIKTSFIDLTQIDKLKTGITDKTTLIYFETPVNPNLEIIDIKEVSQLCSSHNIKVV
HHHCCCCCCEECHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEHHHHHHHHCCCCEEEE
VDNTFATPFYQNPLKLGADVVIHSATKYLNGHGDVLAGAVIARDQEYIHELKFNYLTELG
EECCCCCCCCCCHHHHCCHHEEEHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHH
GVLSPFNSWLVLRGLKTLGLRMREHEKNASKIAKYLKNHPGINRVYYPGLQDFKGYEIAK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHH
KQMSGFGAIISFEIMGGYQDIKRAVESLNLIKLAVSLGDAETLIEYPFGMTHRDYSGQEL
HHHCCCCEEEEEEEECCHHHHHHHHHHHHHHHEEECCCCHHHHHHCCCCCCCCCCCHHHH
ARMGLNKNMVRLSVGLENYEDIIADLDQALSK
HHHCCCCCEEEEEECCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]