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The map label for this gene is truB [H]

Identifier: 220931631

GI number: 220931631

Start: 845629

End: 846540

Strand: Direct

Name: truB [H]

Synonym: Hore_07870

Alternate gene names: 220931631

Gene position: 845629-846540 (Clockwise)

Preceding gene: 220931630

Following gene: 220931632

Centisome position: 32.8

GC content: 36.62

Gene sequence:

>912_bases
ATGTCTAGGGTAGATGGTATTATCAGTATAATAAAACCTCCGGGAATGACTTCCTTTGATGTTGTTGCCTGGATCAGGAA
AATGTATTCTGTAAAAAAAGCAGGGCATACCGGAACCCTGGATCCTGCAGCCAGTGGTGTATTACCGGTATGTCTGGGTA
AAGGCACAAAAATCATACCTTATTTGCCGGATGAAGTAAAGGGATATGTTGCTGAAATAACCCTGGGGGTTAAGACCGAT
ACTTTAGATTCAGAAGGTCAGATCATTGAAGAAAACCAGAAATGGGAGTCAATTACTGATAGTCATTTAAATAAGGTGAT
TTCTGATTTTCAAGGGGTAATTGAACAGGTTCCTCCCATGTATTCTGCCGTCAAATATAAAGGAAAGAGGTTATATGAAC
TTGCCCGGGAGGGTAAAAAGATAAAAAGAGAACCAAGGCAGGTTGAGATAAAAAAAATAAATATTCTTGATATAGACCTT
CCTAAAGTAAAATTATTTGTTTTATGCTCTCCAGGAACCTATATCAGGTCAATTGCCCGTGATATTGGAGAAAAGCTTGG
CTGTGGAGCTTACCTGTCATTTCTGGTAAGAACCAGATCAGGTTCGTTTAATTTAGAAAATAGTTTTACGTTATCTGAAA
TAAAATTCAGGGGGGATAAATTATTATATAATTTTGACTATCCCCTTACTAACCATTTTACCAGAATCGATATCAGGAGA
GGTTCGCGTCAGAAGGTTTATAATGGTGCTCCCCTGTATGGAAGTGATATAGCAAAAATATATGGTAAAGTTACTTTAAG
GGATAAAGTATTGATTTATAATAATAATACTTTTCTGGCCATATACCGGGTTAGTGATGTAGCAGGAAATCAGATTAACT
TAAACGCGGAACGTGTATTTAAAACATCATAA

Upstream 100 bases:

>100_bases
GCAGAATTTGGGGGTGGGGGTCACCCCAGGGCAGCTGGCTGTACTATAAATAATACCCTTGGAAATGCTATAAAAATGGT
TTTAAAAAAGGTGAAGAAAT

Downstream 100 bases:

>100_bases
GTTGCTAGAGTACTTCTAGGGTGATGATAATGAAAATAGTACAAAGCTCAAAATTTGGATTAATCAACAAAGAACCGGTA
GTTGTCGCACTGGGTTCGTT

Product: tRNA pseudouridine 55 synthase

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MSRVDGIISIIKPPGMTSFDVVAWIRKMYSVKKAGHTGTLDPAASGVLPVCLGKGTKIIPYLPDEVKGYVAEITLGVKTD
TLDSEGQIIEENQKWESITDSHLNKVISDFQGVIEQVPPMYSAVKYKGKRLYELAREGKKIKREPRQVEIKKINILDIDL
PKVKLFVLCSPGTYIRSIARDIGEKLGCGAYLSFLVRTRSGSFNLENSFTLSEIKFRGDKLLYNFDYPLTNHFTRIDIRR
GSRQKVYNGAPLYGSDIAKIYGKVTLRDKVLIYNNNTFLAIYRVSDVAGNQINLNAERVFKTS

Sequences:

>Translated_303_residues
MSRVDGIISIIKPPGMTSFDVVAWIRKMYSVKKAGHTGTLDPAASGVLPVCLGKGTKIIPYLPDEVKGYVAEITLGVKTD
TLDSEGQIIEENQKWESITDSHLNKVISDFQGVIEQVPPMYSAVKYKGKRLYELAREGKKIKREPRQVEIKKINILDIDL
PKVKLFVLCSPGTYIRSIARDIGEKLGCGAYLSFLVRTRSGSFNLENSFTLSEIKFRGDKLLYNFDYPLTNHFTRIDIRR
GSRQKVYNGAPLYGSDIAKIYGKVTLRDKVLIYNNNTFLAIYRVSDVAGNQINLNAERVFKTS
>Mature_302_residues
SRVDGIISIIKPPGMTSFDVVAWIRKMYSVKKAGHTGTLDPAASGVLPVCLGKGTKIIPYLPDEVKGYVAEITLGVKTDT
LDSEGQIIEENQKWESITDSHLNKVISDFQGVIEQVPPMYSAVKYKGKRLYELAREGKKIKREPRQVEIKKINILDIDLP
KVKLFVLCSPGTYIRSIARDIGEKLGCGAYLSFLVRTRSGSFNLENSFTLSEIKFRGDKLLYNFDYPLTNHFTRIDIRRG
SRQKVYNGAPLYGSDIAKIYGKVTLRDKVLIYNNNTFLAIYRVSDVAGNQINLNAERVFKTS

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=241, Percent_Identity=35.2697095435685, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI4503337, Length=201, Percent_Identity=30.3482587064677, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI215599015, Length=240, Percent_Identity=27.9166666666667, Blast_Score=76, Evalue=4e-14,
Organism=Escherichia coli, GI2367200, Length=212, Percent_Identity=41.5094339622642, Blast_Score=182, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI17553978, Length=210, Percent_Identity=28.5714285714286, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6324037, Length=152, Percent_Identity=42.1052631578947, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6323204, Length=282, Percent_Identity=26.9503546099291, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI281364189, Length=201, Percent_Identity=29.8507462686567, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI281364187, Length=201, Percent_Identity=29.8507462686567, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI281364185, Length=201, Percent_Identity=29.8507462686567, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI281364183, Length=201, Percent_Identity=29.8507462686567, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI62471759, Length=201, Percent_Identity=29.8507462686567, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI17975520, Length=201, Percent_Identity=29.8507462686567, Blast_Score=85, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780 [H]

Pfam domain/function: PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 34068; Mature: 33937

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRVDGIISIIKPPGMTSFDVVAWIRKMYSVKKAGHTGTLDPAASGVLPVCLGKGTKIIP
CCCHHCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCEEEC
YLPDEVKGYVAEITLGVKTDTLDSEGQIIEENQKWESITDSHLNKVISDFQGVIEQVPPM
CCCHHHCCEEEEEEEEEEECCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCH
YSAVKYKGKRLYELAREGKKIKREPRQVEIKKINILDIDLPKVKLFVLCSPGTYIRSIAR
HHHHHHCCHHHHHHHHCCHHHHCCCCEEEEEEEEEEEECCCCEEEEEEECCCHHHHHHHH
DIGEKLGCGAYLSFLVRTRSGSFNLENSFTLSEIKFRGDKLLYNFDYPLTNHFTRIDIRR
HHHHHCCHHHHHHHHHHHCCCCCCCCCCEEHHHEEECCCEEEEEECCCCCCCEEEEEECC
GSRQKVYNGAPLYGSDIAKIYGKVTLRDKVLIYNNNTFLAIYRVSDVAGNQINLNAERVF
CCCCCCCCCCCCCCCHHHHHHEEEEEEEEEEEEECCEEEEEEEEECCCCCEEECCHHHEE
KTS
ECC
>Mature Secondary Structure 
SRVDGIISIIKPPGMTSFDVVAWIRKMYSVKKAGHTGTLDPAASGVLPVCLGKGTKIIP
CCHHCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCEEEC
YLPDEVKGYVAEITLGVKTDTLDSEGQIIEENQKWESITDSHLNKVISDFQGVIEQVPPM
CCCHHHCCEEEEEEEEEEECCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCH
YSAVKYKGKRLYELAREGKKIKREPRQVEIKKINILDIDLPKVKLFVLCSPGTYIRSIAR
HHHHHHCCHHHHHHHHCCHHHHCCCCEEEEEEEEEEEECCCCEEEEEEECCCHHHHHHHH
DIGEKLGCGAYLSFLVRTRSGSFNLENSFTLSEIKFRGDKLLYNFDYPLTNHFTRIDIRR
HHHHHCCHHHHHHHHHHHCCCCCCCCCCEEHHHEEECCCEEEEEECCCCCCCEEEEEECC
GSRQKVYNGAPLYGSDIAKIYGKVTLRDKVLIYNNNTFLAIYRVSDVAGNQINLNAERVF
CCCCCCCCCCCCCCCHHHHHHEEEEEEEEEEEEECCEEEEEEEEECCCCCEEECCHHHEE
KTS
ECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA