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The map label for this gene is trmD

Identifier: 220931577

GI number: 220931577

Start: 791773

End: 792525

Strand: Direct

Name: trmD

Synonym: Hore_07330

Alternate gene names: 220931577

Gene position: 791773-792525 (Clockwise)

Preceding gene: 220931576

Following gene: 220931578

Centisome position: 30.71

GC content: 40.77

Gene sequence:

>753_bases
ATGAATTTTGATATTTTAACTTTATTTCCAGAGATGTTTGACGGCCCTTTTAACAATAGTATTATTGGTAAAGCCCGGGA
CAGGGGTTTAATTGATATCAATTTGATAGATATTAGGGATTATACCACTGATAAGCACAACACGGCCGATGATTACCCCT
ATGGGGGTGGGGCCGGTATGGTAATGAAGGTAGAACCGATTTATCGGGCTTTGACTGATGTGAGAAGTAAGCGAAAAGGG
GAATACCCTGTTGTCTTACTTAGCCCCCAGGGTAAAACCCTTGATCAGAATCTGGTTAAATCATTGAGCAAACATGATGG
CCTCATATTAATATGTGGTCATTATGAAGGTGTTGATGAAAGGGTACGCCAGATTTTTTCTCCTCTGGAGATTTCTATTG
GAGATTATGTGGTTACCGGGGGTGAAATTGGGGCTATTGTCCTTGTTGATGCAGTTTCCAGGATGATACCGGGGGTTCTC
GGTGATGAACGCTCCAAAGTAGAGGATTCTTTTTACAATGGTATTCTGGATTACCCCCATTATACCCGTCCCCGGGTTTA
TCAAAACTTAAAGGTTCCTGATGTTTTGTTAAGTGGGAACCATGGCCTGATTGAAAAATGGCGTAAAAAGCAGGCTTTAA
AAAGAACCCTGATTAGAAGGCCTGATCTTCTTAAAAATAAAAAACTTACTCCGGAGGAAGAAGAGTTACTTGAAGAAGCA
AAGGCAGAAATAGAAGGTGAATACAATGACTAA

Upstream 100 bases:

>100_bases
TTCCAGCTGCCCGGGAAATAGTAGTCAATATTAACCCGGATGAAGAGAAGATAATTATTAAGCCCATTCCAGGGTTACTG
GATTTGTAGGGGGGCCAGTC

Downstream 100 bases:

>100_bases
AGATCACCCCAAGATATATCTGGGACTTCTTCACTATCCAATCTATAATAAACAAAAAAAGGTTATAACAACGACTATAA
CAAACCTCGATGTTCATGAC

Product: tRNA (guanine-N1)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MNFDILTLFPEMFDGPFNNSIIGKARDRGLIDINLIDIRDYTTDKHNTADDYPYGGGAGMVMKVEPIYRALTDVRSKRKG
EYPVVLLSPQGKTLDQNLVKSLSKHDGLILICGHYEGVDERVRQIFSPLEISIGDYVVTGGEIGAIVLVDAVSRMIPGVL
GDERSKVEDSFYNGILDYPHYTRPRVYQNLKVPDVLLSGNHGLIEKWRKKQALKRTLIRRPDLLKNKKLTPEEEELLEEA
KAEIEGEYND

Sequences:

>Translated_250_residues
MNFDILTLFPEMFDGPFNNSIIGKARDRGLIDINLIDIRDYTTDKHNTADDYPYGGGAGMVMKVEPIYRALTDVRSKRKG
EYPVVLLSPQGKTLDQNLVKSLSKHDGLILICGHYEGVDERVRQIFSPLEISIGDYVVTGGEIGAIVLVDAVSRMIPGVL
GDERSKVEDSFYNGILDYPHYTRPRVYQNLKVPDVLLSGNHGLIEKWRKKQALKRTLIRRPDLLKNKKLTPEEEELLEEA
KAEIEGEYND
>Mature_250_residues
MNFDILTLFPEMFDGPFNNSIIGKARDRGLIDINLIDIRDYTTDKHNTADDYPYGGGAGMVMKVEPIYRALTDVRSKRKG
EYPVVLLSPQGKTLDQNLVKSLSKHDGLILICGHYEGVDERVRQIFSPLEISIGDYVVTGGEIGAIVLVDAVSRMIPGVL
GDERSKVEDSFYNGILDYPHYTRPRVYQNLKVPDVLLSGNHGLIEKWRKKQALKRTLIRRPDLLKNKKLTPEEEELLEEA
KAEIEGEYND

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=243, Percent_Identity=51.440329218107, Blast_Score=246, Evalue=1e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_HALOH (B8CW21)

Other databases:

- EMBL:   CP001098
- RefSeq:   YP_002508485.1
- GeneID:   7312968
- GenomeReviews:   CP001098_GR
- KEGG:   hor:Hore_07330
- HOGENOM:   HBG285805
- OMA:   NRYDLYL
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 28292; Mature: 28292

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: NA

Important sites: BINDING 113-113

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFDILTLFPEMFDGPFNNSIIGKARDRGLIDINLIDIRDYTTDKHNTADDYPYGGGAGM
CCCCCEEECHHHHCCCCCCCEECCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCE
VMKVEPIYRALTDVRSKRKGEYPVVLLSPQGKTLDQNLVKSLSKHDGLILICGHYEGVDE
EEEEHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHCCCEEEEECCCCCHHH
RVRQIFSPLEISIGDYVVTGGEIGAIVLVDAVSRMIPGVLGDERSKVEDSFYNGILDYPH
HHHHHHCCCEEECCCEEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCC
YTRPRVYQNLKVPDVLLSGNHGLIEKWRKKQALKRTLIRRPDLLKNKKLTPEEEELLEEA
CCCCHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCHHHHHHHH
KAEIEGEYND
HHHCCCCCCC
>Mature Secondary Structure
MNFDILTLFPEMFDGPFNNSIIGKARDRGLIDINLIDIRDYTTDKHNTADDYPYGGGAGM
CCCCCEEECHHHHCCCCCCCEECCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCE
VMKVEPIYRALTDVRSKRKGEYPVVLLSPQGKTLDQNLVKSLSKHDGLILICGHYEGVDE
EEEEHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHCCCEEEEECCCCCHHH
RVRQIFSPLEISIGDYVVTGGEIGAIVLVDAVSRMIPGVLGDERSKVEDSFYNGILDYPH
HHHHHHCCCEEECCCEEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCC
YTRPRVYQNLKVPDVLLSGNHGLIEKWRKKQALKRTLIRRPDLLKNKKLTPEEEELLEEA
CCCCHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCHHHHHHHH
KAEIEGEYND
HHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA