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The map label for this gene is sigF [H]

Identifier: 220931546

GI number: 220931546

Start: 761241

End: 762014

Strand: Direct

Name: sigF [H]

Synonym: Hore_07020

Alternate gene names: 220931546

Gene position: 761241-762014 (Clockwise)

Preceding gene: 220931545

Following gene: 220931547

Centisome position: 29.53

GC content: 34.75

Gene sequence:

>774_bases
ATGGAATACAGTATTATCAACCTTCCCAAAGTAAGGTTGTTAAGTGAGGAGGAGACCAGGCATTACATTAAGAAGGCCCA
GAATAATGACCAAAAGGCCCTGGATAAGTTAGTTGAACATAATTTAAGACTGGTCCTGAAAGTAATAAATCGTTTCAAAA
GCTCTGGTTATGATCCCCAGGATTTATTTCAGATCGGGGTTATTGGATTAATCAAGGCTATAAAAAATTTTGATTTAGAC
CGGGGAGTAAAGTTCTCAACATATGCTGTGTCCAGGATTATTGGAGAAATTAGATTACATATCAGGGATGATGGGATAAT
AAAGGTTAGCCGCTCCCTTAAGAAAATTGCTAATGATGTCAGAAAAAAAGAAGAAGAATTAAAAAAGAAAAATAATTGTT
CTCCCAGTATTTTAGAACTTTCAAAAAAAACTGGTTATAGTAAGGAAGAGATTGTCCAGGCCCTTGAAGCCAATAAAGCC
CCAACTTCTATATATGAAACAGTTTATGAAGATGATGGTTCTGAATTATATGTTGTGGATAATCTTGCTAACGAGAGTAA
AGATGAAGAAAAGATACCTGATTTCAATAAAATTTTGCTGGTTGACCTGTTAAAAAAACTTGATGAACGGTCACGAAAAA
TTATTTACCTGCGTTATTTTGAGGACAAAACCCAGCAGGAAGTAGCTGATGAAATAGGGGTATCCCAGGTTCAAATATCA
CGGCTTGAGAAAAAAATACTTCATAAGTTAAGGGATAATCTTGAAGAATCTTAA

Upstream 100 bases:

>100_bases
GATTTTAAACTGGAATCAGAGTTGAATAAAGGAACCAGAGTTATAATGGTAAAAGTTCCGGAACGTTATAAGCAGGTAAA
GAAATAAGGAGAGGAATAAA

Downstream 100 bases:

>100_bases
ATTTCAATCAGGTATTTTCTTACTGGGTTTCAGGGTTGTATGTTATTATTAAAAGTTAATCTGGTATTCAAGCTTGTATA
ATTTTTTTAAATATTCCCCG

Product: sporulation sigma factor SigF

Products: NA

Alternate protein names: Sporulation sigma factor; Stage II sporulation protein AC [H]

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MEYSIINLPKVRLLSEEETRHYIKKAQNNDQKALDKLVEHNLRLVLKVINRFKSSGYDPQDLFQIGVIGLIKAIKNFDLD
RGVKFSTYAVSRIIGEIRLHIRDDGIIKVSRSLKKIANDVRKKEEELKKKNNCSPSILELSKKTGYSKEEIVQALEANKA
PTSIYETVYEDDGSELYVVDNLANESKDEEKIPDFNKILLVDLLKKLDERSRKIIYLRYFEDKTQQEVADEIGVSQVQIS
RLEKKILHKLRDNLEES

Sequences:

>Translated_257_residues
MEYSIINLPKVRLLSEEETRHYIKKAQNNDQKALDKLVEHNLRLVLKVINRFKSSGYDPQDLFQIGVIGLIKAIKNFDLD
RGVKFSTYAVSRIIGEIRLHIRDDGIIKVSRSLKKIANDVRKKEEELKKKNNCSPSILELSKKTGYSKEEIVQALEANKA
PTSIYETVYEDDGSELYVVDNLANESKDEEKIPDFNKILLVDLLKKLDERSRKIIYLRYFEDKTQQEVADEIGVSQVQIS
RLEKKILHKLRDNLEES
>Mature_257_residues
MEYSIINLPKVRLLSEEETRHYIKKAQNNDQKALDKLVEHNLRLVLKVINRFKSSGYDPQDLFQIGVIGLIKAIKNFDLD
RGVKFSTYAVSRIIGEIRLHIRDDGIIKVSRSLKKIANDVRKKEEELKKKNNCSPSILELSKKTGYSKEEIVQALEANKA
PTSIYETVYEDDGSELYVVDNLANESKDEEKIPDFNKILLVDLLKKLDERSRKIIYLRYFEDKTQQEVADEIGVSQVQIS
RLEKKILHKLRDNLEES

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=251, Percent_Identity=28.6852589641434, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1789448, Length=231, Percent_Identity=28.5714285714286, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1789871, Length=263, Percent_Identity=28.5171102661597, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788231, Length=198, Percent_Identity=26.2626262626263, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014322
- InterPro:   IPR014236
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 29822; Mature: 29822

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYSIINLPKVRLLSEEETRHYIKKAQNNDQKALDKLVEHNLRLVLKVINRFKSSGYDPQ
CCCCCCCCCHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
DLFQIGVIGLIKAIKNFDLDRGVKFSTYAVSRIIGEIRLHIRDDGIIKVSRSLKKIANDV
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHH
RKKEEELKKKNNCSPSILELSKKTGYSKEEIVQALEANKAPTSIYETVYEDDGSELYVVD
HHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEE
NLANESKDEEKIPDFNKILLVDLLKKLDERSRKIIYLRYFEDKTQQEVADEIGVSQVQIS
CCCCCCCCHHHCCCHHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHCHHHHHHH
RLEKKILHKLRDNLEES
HHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEYSIINLPKVRLLSEEETRHYIKKAQNNDQKALDKLVEHNLRLVLKVINRFKSSGYDPQ
CCCCCCCCCHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
DLFQIGVIGLIKAIKNFDLDRGVKFSTYAVSRIIGEIRLHIRDDGIIKVSRSLKKIANDV
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHH
RKKEEELKKKNNCSPSILELSKKTGYSKEEIVQALEANKAPTSIYETVYEDDGSELYVVD
HHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEE
NLANESKDEEKIPDFNKILLVDLLKKLDERSRKIIYLRYFEDKTQQEVADEIGVSQVQIS
CCCCCCCCHHHCCCHHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHCHHHHHHH
RLEKKILHKLRDNLEES
HHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2513372 [H]