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The map label for this gene is ldh [H]

Identifier: 220931519

GI number: 220931519

Start: 733000

End: 734061

Strand: Direct

Name: ldh [H]

Synonym: Hore_06750

Alternate gene names: 220931519

Gene position: 733000-734061 (Clockwise)

Preceding gene: 220931518

Following gene: 220931520

Centisome position: 28.43

GC content: 40.3

Gene sequence:

>1062_bases
ATGAAAATATTTGATTATCTAGAAAAATATGATTATGAACAGCTGGTTTTTAATTATGATGAGGCTTCCGGGTTAAAGGC
AATCATTGCTATCCATGACACGACTTTAGGACCTGCCCTTGGTGGAACAAGAATGTGGTATTATGAAAAAGAAGAAGATG
CCATAATTGATGTTCTCCGCCTGTCAAGGGGTATGACCTATAAATCTGCGGCAGCCGGTTTGAACCTGGGTGGTGGTAAA
GCTGTAATTATAGGTGACCCTAAAAAGCATAAAAGTGAAGAACTATGGAGGGCCTTTGGTCGTTTTGTCCAGAGTTTAAA
TGGTAGGTATATAACTGCAGAAGATGTGGGAACATCAGTTGATGATATGGCAATTGTCGCCCAGGAAACTGACTTTGTTA
CCGGTCTTCCCGGAACCAGTGGTGACCCATCTCCAGTTACTGCCCTTGGCGTCTGGCGTGGCATGAAAGCTGCTGCTGAT
GAGGTATATGGTACTCCTGATCTTAAAGGTAAAGTTGTAGCTATTCAGGGTCTGGGTCATGTAGGTTATTACCTTGCTAA
ACACATCGTAGAAGAAGGCGGTAAACTGATTGTTACCGATATTGATAAAGAGAAAATTAAAAAGGTTGTAGATGATTTTG
GTGCTGAAGTAGTTGAACCTGATGAAATCTACGGTGTTGAGTGTGATATTTTTGCACCATGTGCTCTGGGGGCTGTTATA
AATGATGAAACTTTACCCCAGTTTAAGTGTGATATTGTAGCTGGAGCTGCCAATAATGTTCTGGCTGAAGAAAGAAATGG
AGATATTCTTGAAGAAAAAGGAATCCTCTATGCACCTGATTATGTTATCAATGCCGGTGGGGTAATTAATGTATATCAGG
AACTTATAGGCTATAACCGGGAACGGGCTCTGGAGAAGGCCAATAAAATTTATGACAATATTAAGAAAGTTATTGAGATC
AGTAAACGGGATAACATCCCCACCTATAAGGCTGCAGATATAATGGCAGAAGAACGTATCGCTAAAATTGGTAAAGTAAG
AAAAAATTATATACCCAGGTAG

Upstream 100 bases:

>100_bases
CTTCACGTGCTGATAGAGCCAGGGTAAAATTAAATTCAATTGCCCTTGCTAAACTTGTAGTCCAGGGTTTAAAAAATAAT
AATTAAGGAGGAATTATCCT

Downstream 100 bases:

>100_bases
GAGGATATAATCATGGCAGAAATGATCTTGGCTATAAATCCTGGATCAACCTCTACTAAGCTTGCTCTTTTTGATGGAAT
AGAAGAAGTATTCACCGAAA

Product: Glu/Leu/Phe/Val dehydrogenase

Products: NA

Alternate protein names: LeuDH [H]

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MKIFDYLEKYDYEQLVFNYDEASGLKAIIAIHDTTLGPALGGTRMWYYEKEEDAIIDVLRLSRGMTYKSAAAGLNLGGGK
AVIIGDPKKHKSEELWRAFGRFVQSLNGRYITAEDVGTSVDDMAIVAQETDFVTGLPGTSGDPSPVTALGVWRGMKAAAD
EVYGTPDLKGKVVAIQGLGHVGYYLAKHIVEEGGKLIVTDIDKEKIKKVVDDFGAEVVEPDEIYGVECDIFAPCALGAVI
NDETLPQFKCDIVAGAANNVLAEERNGDILEEKGILYAPDYVINAGGVINVYQELIGYNRERALEKANKIYDNIKKVIEI
SKRDNIPTYKAADIMAEERIAKIGKVRKNYIPR

Sequences:

>Translated_353_residues
MKIFDYLEKYDYEQLVFNYDEASGLKAIIAIHDTTLGPALGGTRMWYYEKEEDAIIDVLRLSRGMTYKSAAAGLNLGGGK
AVIIGDPKKHKSEELWRAFGRFVQSLNGRYITAEDVGTSVDDMAIVAQETDFVTGLPGTSGDPSPVTALGVWRGMKAAAD
EVYGTPDLKGKVVAIQGLGHVGYYLAKHIVEEGGKLIVTDIDKEKIKKVVDDFGAEVVEPDEIYGVECDIFAPCALGAVI
NDETLPQFKCDIVAGAANNVLAEERNGDILEEKGILYAPDYVINAGGVINVYQELIGYNRERALEKANKIYDNIKKVIEI
SKRDNIPTYKAADIMAEERIAKIGKVRKNYIPR
>Mature_353_residues
MKIFDYLEKYDYEQLVFNYDEASGLKAIIAIHDTTLGPALGGTRMWYYEKEEDAIIDVLRLSRGMTYKSAAAGLNLGGGK
AVIIGDPKKHKSEELWRAFGRFVQSLNGRYITAEDVGTSVDDMAIVAQETDFVTGLPGTSGDPSPVTALGVWRGMKAAAD
EVYGTPDLKGKVVAIQGLGHVGYYLAKHIVEEGGKLIVTDIDKEKIKKVVDDFGAEVVEPDEIYGVECDIFAPCALGAVI
NDETLPQFKCDIVAGAANNVLAEERNGDILEEKGILYAPDYVINAGGVINVYQELIGYNRERALEKANKIYDNIKKVIEI
SKRDNIPTYKAADIMAEERIAKIGKVRKNYIPR

Specific function: Functions catabolically in the bacterial metabolism of branched-chain L-amino acids, and plays an important role in spore germination in cooperation with alanine dehydrogenase [H]

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=312, Percent_Identity=27.5641025641026, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI4885281, Length=312, Percent_Identity=25.9615384615385, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17544676, Length=319, Percent_Identity=27.2727272727273, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI45549226, Length=326, Percent_Identity=28.5276073619632, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI45553475, Length=326, Percent_Identity=28.5276073619632, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24649283, Length=286, Percent_Identity=28.3216783216783, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24649460, Length=279, Percent_Identity=28.3154121863799, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR016211
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.9 [H]

Molecular weight: Translated: 38796; Mature: 38796

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIFDYLEKYDYEQLVFNYDEASGLKAIIAIHDTTLGPALGGTRMWYYEKEEDAIIDVLR
CCHHHHHHHCCHHHHEEECCCCCCCEEEEEEECCCCCCCCCCCEEEEEECCCHHHHHHHH
LSRGMTYKSAAAGLNLGGGKAVIIGDPKKHKSEELWRAFGRFVQSLNGRYITAEDVGTSV
HHCCCCCHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEHHHCCCCH
DDMAIVAQETDFVTGLPGTSGDPSPVTALGVWRGMKAAADEVYGTPDLKGKVVAIQGLGH
HHEEEEEECCCEEECCCCCCCCCCCCHHHHHHHCHHHHHHHHCCCCCCCCEEEEEECCCH
VGYYLAKHIVEEGGKLIVTDIDKEKIKKVVDDFGAEVVEPDEIYGVECDIFAPCALGAVI
HHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHCCCCCCCCCEECCEECEECHHHHHHHC
NDETLPQFKCDIVAGAANNVLAEERNGDILEEKGILYAPDYVINAGGVINVYQELIGYNR
CCCCCCCEEEEEEECCCCCCHHHCCCCCEECCCCCEECCCEEEECCCHHHHHHHHHCCCH
ERALEKANKIYDNIKKVIEISKRDNIPTYKAADIMAEERIAKIGKVRKNYIPR
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKIFDYLEKYDYEQLVFNYDEASGLKAIIAIHDTTLGPALGGTRMWYYEKEEDAIIDVLR
CCHHHHHHHCCHHHHEEECCCCCCCEEEEEEECCCCCCCCCCCEEEEEECCCHHHHHHHH
LSRGMTYKSAAAGLNLGGGKAVIIGDPKKHKSEELWRAFGRFVQSLNGRYITAEDVGTSV
HHCCCCCHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEHHHCCCCH
DDMAIVAQETDFVTGLPGTSGDPSPVTALGVWRGMKAAADEVYGTPDLKGKVVAIQGLGH
HHEEEEEECCCEEECCCCCCCCCCCCHHHHHHHCHHHHHHHHCCCCCCCCEEEEEECCCH
VGYYLAKHIVEEGGKLIVTDIDKEKIKKVVDDFGAEVVEPDEIYGVECDIFAPCALGAVI
HHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHCCCCCCCCCEECCEECEECHHHHHHHC
NDETLPQFKCDIVAGAANNVLAEERNGDILEEKGILYAPDYVINAGGVINVYQELIGYNR
CCCCCCCEEEEEEECCCCCCHHHCCCCCEECCCCCEECCCEEEECCCHHHHHHHHHCCCH
ERALEKANKIYDNIKKVIEISKRDNIPTYKAADIMAEERIAKIGKVRKNYIPR
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9188201 [H]