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The map label for this gene is pilT [H]

Identifier: 220931442

GI number: 220931442

Start: 650670

End: 651752

Strand: Direct

Name: pilT [H]

Synonym: Hore_05980

Alternate gene names: 220931442

Gene position: 650670-651752 (Clockwise)

Preceding gene: 220931441

Following gene: 220931443

Centisome position: 25.24

GC content: 42.29

Gene sequence:

>1083_bases
ATGGTATTGATGGATATCAAAGAATTTTTAAAAAAATTTGTAGAAGACCAACATATTTCTGATCTTCATCTTACAGTTAA
TTCTAAACCTGTAGTCAGATATGACGGAAAACTCAAAGTATTTGAAGAATTTTCGGGTCGGATTGGGTTTGAGGATATGC
AGAATATTACTAAAGAACTAATGACAGATGACCAGTGGCAACAATTTCAGGAAAAGGGTGAGCTTGATTTTTCCTATAGT
GTTCCCGGTTTTTCCCGGTTCCGGGTTAATGCTTATTACCAGAGGGGGGCTATTGCCCTGGCTTTGAGGATAATCCCGAC
AAAAATACCAACCATCGAAGAGCTGGGTCTACCGCAAATTTTAAAAAACCTGGCCCTGATGAGAAAGGGACTGTTCCTGT
GTACCGGCCCTACTGGAAGCGGGAAGACAACAACCCTGGCTTCTATGATTAATATTATCAATAAAAATAGACAGGCCCAC
ATCCTGACCCTTGAAGACCCCATAGAATATTTACACCGCCATAATAAAAGTATTGTTCATCAAAGGGAAGTGGGGATTGA
TACTACAAGTTTTAAAGAAGGGTTGAGATCTGCCCTGAGGCAGGACCCTGACGTTATTCTGGTTGGGGAGATGAGGGATC
ATGAAACAATATCCATTGCCTTAGAAGCAGCCGAGACCGGTCATCTTGTTCTGGCAACATTACATACGAATGATGCTCCC
CAGACCATTGATCGTATTATAGATGTTTTTCCTCCCCAACAGCAACGACAGGTCAGGGTGCAGTTATCACTTACTCTTAA
CGGGATTGTTTCCCAGCAATTGCTGCCCCGGAAGGATGGTAATGGCAGGATAGCAGCCCTGGAGGTATTAATTGGAACCC
CGGCTGTCAGAAATGTCATCAGGGATGGAAAGAGTCATCAACTTTATTCCAGTATGCAGACCGGTGGGAAATACGGAATG
ATCACCATGGACAACTATATACTTGATTTATTAAGTAAAAGTCTTATTAATAAGGATACTGCCCTGAGAAGGGCCAATGA
CCCGGAATATATCAAAAAGAGGATTAATGCGGTCAGGGTTTAA

Upstream 100 bases:

>100_bases
CCCGGAAAGCAGGAATGCTAACTTTAAAAGAAGATGGGGTTAATAAGGTAATAGAAGGGTTAACTTCATATGAAGAACTG
GCAAGGGTAGTTAGTTAGGA

Downstream 100 bases:

>100_bases
ACTGGTTTAACTATTTATCTTGTTCTTCCCATAATTGATTTTTCTAGTCGTCAAACTTATAAATTTTGTTAATTATGAAA
AGCCAATAAACTAAAAGGGA

Product: Tfp pilus assembly protein, pilus retraction ATPase PilT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MVLMDIKEFLKKFVEDQHISDLHLTVNSKPVVRYDGKLKVFEEFSGRIGFEDMQNITKELMTDDQWQQFQEKGELDFSYS
VPGFSRFRVNAYYQRGAIALALRIIPTKIPTIEELGLPQILKNLALMRKGLFLCTGPTGSGKTTTLASMINIINKNRQAH
ILTLEDPIEYLHRHNKSIVHQREVGIDTTSFKEGLRSALRQDPDVILVGEMRDHETISIALEAAETGHLVLATLHTNDAP
QTIDRIIDVFPPQQQRQVRVQLSLTLNGIVSQQLLPRKDGNGRIAALEVLIGTPAVRNVIRDGKSHQLYSSMQTGGKYGM
ITMDNYILDLLSKSLINKDTALRRANDPEYIKKRINAVRV

Sequences:

>Translated_360_residues
MVLMDIKEFLKKFVEDQHISDLHLTVNSKPVVRYDGKLKVFEEFSGRIGFEDMQNITKELMTDDQWQQFQEKGELDFSYS
VPGFSRFRVNAYYQRGAIALALRIIPTKIPTIEELGLPQILKNLALMRKGLFLCTGPTGSGKTTTLASMINIINKNRQAH
ILTLEDPIEYLHRHNKSIVHQREVGIDTTSFKEGLRSALRQDPDVILVGEMRDHETISIALEAAETGHLVLATLHTNDAP
QTIDRIIDVFPPQQQRQVRVQLSLTLNGIVSQQLLPRKDGNGRIAALEVLIGTPAVRNVIRDGKSHQLYSSMQTGGKYGM
ITMDNYILDLLSKSLINKDTALRRANDPEYIKKRINAVRV
>Mature_360_residues
MVLMDIKEFLKKFVEDQHISDLHLTVNSKPVVRYDGKLKVFEEFSGRIGFEDMQNITKELMTDDQWQQFQEKGELDFSYS
VPGFSRFRVNAYYQRGAIALALRIIPTKIPTIEELGLPQILKNLALMRKGLFLCTGPTGSGKTTTLASMINIINKNRQAH
ILTLEDPIEYLHRHNKSIVHQREVGIDTTSFKEGLRSALRQDPDVILVGEMRDHETISIALEAAETGHLVLATLHTNDAP
QTIDRIIDVFPPQQQRQVRVQLSLTLNGIVSQQLLPRKDGNGRIAALEVLIGTPAVRNVIRDGKSHQLYSSMQTGGKYGM
ITMDNYILDLLSKSLINKDTALRRANDPEYIKKRINAVRV

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=320, Percent_Identity=46.25, Blast_Score=287, Evalue=7e-79,
Organism=Escherichia coli, GI1789723, Length=160, Percent_Identity=46.25, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1786296, Length=271, Percent_Identity=33.9483394833948, Blast_Score=110, Evalue=9e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 40756; Mature: 40756

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLMDIKEFLKKFVEDQHISDLHLTVNSKPVVRYDGKLKVFEEFSGRIGFEDMQNITKEL
CEEHHHHHHHHHHHCCCCCCEEEEEECCCCEEEECCCEEEEHHHCCCCCHHHHHHHHHHH
MTDDQWQQFQEKGELDFSYSVPGFSRFRVNAYYQRGAIALALRIIPTKIPTIEELGLPQI
HCCHHHHHHHHCCCCEEEECCCCCEEEEEEHHHHCCCEEEEEEECCCCCCCHHHCCHHHH
LKNLALMRKGLFLCTGPTGSGKTTTLASMINIINKNRQAHILTLEDPIEYLHRHNKSIVH
HHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHCCHHHHH
QREVGIDTTSFKEGLRSALRQDPDVILVGEMRDHETISIALEAAETGHLVLATLHTNDAP
HHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEEECCCCCEEEEEEECCCCH
QTIDRIIDVFPPQQQRQVRVQLSLTLNGIVSQQLLPRKDGNGRIAALEVLIGTPAVRNVI
HHHHHHHHCCCCCCCCEEEEEEEEEEHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHHHH
RDGKSHQLYSSMQTGGKYGMITMDNYILDLLSKSLINKDTALRRANDPEYIKKRINAVRV
HCCCCHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCHHHHHHCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MVLMDIKEFLKKFVEDQHISDLHLTVNSKPVVRYDGKLKVFEEFSGRIGFEDMQNITKEL
CEEHHHHHHHHHHHCCCCCCEEEEEECCCCEEEECCCEEEEHHHCCCCCHHHHHHHHHHH
MTDDQWQQFQEKGELDFSYSVPGFSRFRVNAYYQRGAIALALRIIPTKIPTIEELGLPQI
HCCHHHHHHHHCCCCEEEECCCCCEEEEEEHHHHCCCEEEEEEECCCCCCCHHHCCHHHH
LKNLALMRKGLFLCTGPTGSGKTTTLASMINIINKNRQAHILTLEDPIEYLHRHNKSIVH
HHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHCCHHHHH
QREVGIDTTSFKEGLRSALRQDPDVILVGEMRDHETISIALEAAETGHLVLATLHTNDAP
HHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEEECCCCCEEEEEEECCCCH
QTIDRIIDVFPPQQQRQVRVQLSLTLNGIVSQQLLPRKDGNGRIAALEVLIGTPAVRNVI
HHHHHHHHCCCCCCCCEEEEEEEEEEHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHHHH
RDGKSHQLYSSMQTGGKYGMITMDNYILDLLSKSLINKDTALRRANDPEYIKKRINAVRV
HCCCCHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCHHHHHHCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]