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The map label for this gene is ntcA [H]

Identifier: 220931438

GI number: 220931438

Start: 646371

End: 647066

Strand: Direct

Name: ntcA [H]

Synonym: Hore_05940

Alternate gene names: 220931438

Gene position: 646371-647066 (Clockwise)

Preceding gene: 220931437

Following gene: 220931439

Centisome position: 25.07

GC content: 38.22

Gene sequence:

>696_bases
ATGGAAGAGATGCAGAGACGATGTCTTAAAGAATTGATGGTCTTTTCAAAAATGGAAAGGGATGAAGTAGCCCTGATCTG
TGAAAGGGCTTATGAGAAGTGGTTTCGTAAAGGTGAAGTTATCTTTTTTGAAAATGATGATAACCGAAATCTCTATGTTC
TCGTATCAGGCCGGGTAAAATTATCCATGCTTTCAGCTGAAGGGAAGGAAAAGGTATTAACAATTCTCCAGGAAGGGGAT
ATTTTTGGAGAAGTATCCCTGTTCGATCAGGACCCACTCCCGGTAACAGCGGAAGTAATTAAAGATTCTCGCTTAATTGT
GTTACCTGTTAAAGACCTGGAAAATATCATTATGAAAAACCCCAAGGTAGCCATTAAAATTATAGAAGCCCTGGCCAAAA
AGACGCGTTTATTAACTGGCCAGATAAGGGATCTGGTTTTTTATGATGCTGAAGGGAGGCTTGCCAGTCTCTTATTGAGG
TTTGCTAAAGATTTCGGGATAGATGTAAAAAGTGGGACTTTAATTGACCTGGTTTTGACCCATCAGGAAATCGCCAATTT
GCTGGGTTCTTCCCGGGTGACAGTTACTAAATTACTGAATCAGTTTATTGATGAAGGGATTATTAAAACATATAAGCGTA
AGATTGTAATTATGAAACCTGAACAGTTACATGATAAAATGCAAAATATGATTTAG

Upstream 100 bases:

>100_bases
ATATTAAAACGGGTAACTATCTTCTTAAATTTGAGCACATAATTTATCGATATTGAATTATAGTCAGGAAAGCAAAAAAA
TCATAAGAGGTGTCATATCA

Downstream 100 bases:

>100_bases
GGGTGAGGCCTGTGTTACAGAAAGCTAAAACAAAAACCAGAGTTATTAAAGCAGATTTATTATTACTGTTGGTAGTAACT
GTCTGGGGGACAACCTTTCC

Product: putative Crp/Fnr family transcriptional regulator

Products: NA

Alternate protein names: DNA-binding protein VF1; Nitrogen-responsive regulatory protein [H]

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MEEMQRRCLKELMVFSKMERDEVALICERAYEKWFRKGEVIFFENDDNRNLYVLVSGRVKLSMLSAEGKEKVLTILQEGD
IFGEVSLFDQDPLPVTAEVIKDSRLIVLPVKDLENIIMKNPKVAIKIIEALAKKTRLLTGQIRDLVFYDAEGRLASLLLR
FAKDFGIDVKSGTLIDLVLTHQEIANLLGSSRVTVTKLLNQFIDEGIIKTYKRKIVIMKPEQLHDKMQNMI

Sequences:

>Translated_231_residues
MEEMQRRCLKELMVFSKMERDEVALICERAYEKWFRKGEVIFFENDDNRNLYVLVSGRVKLSMLSAEGKEKVLTILQEGD
IFGEVSLFDQDPLPVTAEVIKDSRLIVLPVKDLENIIMKNPKVAIKIIEALAKKTRLLTGQIRDLVFYDAEGRLASLLLR
FAKDFGIDVKSGTLIDLVLTHQEIANLLGSSRVTVTKLLNQFIDEGIIKTYKRKIVIMKPEQLHDKMQNMI
>Mature_231_residues
MEEMQRRCLKELMVFSKMERDEVALICERAYEKWFRKGEVIFFENDDNRNLYVLVSGRVKLSMLSAEGKEKVLTILQEGD
IFGEVSLFDQDPLPVTAEVIKDSRLIVLPVKDLENIIMKNPKVAIKIIEALAKKTRLLTGQIRDLVFYDAEGRLASLLLR
FAKDFGIDVKSGTLIDLVLTHQEIANLLGSSRVTVTKLLNQFIDEGIIKTYKRKIVIMKPEQLHDKMQNMI

Specific function: Has affinity for the xisA upstream region. Binds to a 66 bp region containing three repeats of the consensus recognition sequence 5'-ACATT-3' [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789756, Length=180, Percent_Identity=27.2222222222222, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787595, Length=211, Percent_Identity=24.6445497630332, Blast_Score=68, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR022299
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 26574; Mature: 26574

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEMQRRCLKELMVFSKMERDEVALICERAYEKWFRKGEVIFFENDDNRNLYVLVSGRVK
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEEECCEE
LSMLSAEGKEKVLTILQEGDIFGEVSLFDQDPLPVTAEVIKDSRLIVLPVKDLENIIMKN
EEEECCCCHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHCCCCEEEEEHHHHHHHHHCC
PKVAIKIIEALAKKTRLLTGQIRDLVFYDAEGRLASLLLRFAKDFGIDVKSGTLIDLVLT
CHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHCCCCCCCCEEEHHHH
HQEIANLLGSSRVTVTKLLNQFIDEGIIKTYKRKIVIMKPEQLHDKMQNMI
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCEEEEECCHHHHHHHHHCC
>Mature Secondary Structure
MEEMQRRCLKELMVFSKMERDEVALICERAYEKWFRKGEVIFFENDDNRNLYVLVSGRVK
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEEECCEE
LSMLSAEGKEKVLTILQEGDIFGEVSLFDQDPLPVTAEVIKDSRLIVLPVKDLENIIMKN
EEEECCCCHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHCCCCEEEEEHHHHHHHHHCC
PKVAIKIIEALAKKTRLLTGQIRDLVFYDAEGRLASLLLRFAKDFGIDVKSGTLIDLVLT
CHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHCCCCCCCCEEEHHHH
HQEIANLLGSSRVTVTKLLNQFIDEGIIKTYKRKIVIMKPEQLHDKMQNMI
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCEEEEECCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11114927 [H]