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The map label for this gene is ywlF [H]

Identifier: 220931175

GI number: 220931175

Start: 336778

End: 337254

Strand: Direct

Name: ywlF [H]

Synonym: Hore_03270

Alternate gene names: 220931175

Gene position: 336778-337254 (Clockwise)

Preceding gene: 220931174

Following gene: 220931176

Centisome position: 13.06

GC content: 35.01

Gene sequence:

>477_bases
TTGAAAGTCATTAAAAATAAACAATATGTAATTGGCAGTGATAATGCAGGCTATCCTTTAAAGGAGATCATTAAAGAATT
ACTTGAATCTGAAGGGTTAGAATATGAGGACGTTGGAGTTGATTCAGACCAGGATAAGACGATATATCCTGAGATAGCTG
AAAGAGTTGTTAACAGGATAAAAAAAAGTAACTATGAAAAAGAGGGCATATTAATCTGTGGAACAGGTATTGGAATGGCA
ATCACAGCTAATAAATTCTCCAGAATATATGCTGCAGTTTGTCATGATGTTTATTCGGCAGAACGAGCTAGATTGAGTAA
TGATACTAATGTCATTACTATGGGGGCCCGGGTAATTGGGCCAGTGCTTGCCAAAAAAATTATAAGAGAATGGTTAAGTC
TTGAATTTAAACCAGGTCGGTCGACACCTAAGCTAAAGCAAATCAAGAAATATGAAAATCAAAATTTTGTGAGTTAA

Upstream 100 bases:

>100_bases
GTCCTGGATGCAGGAGCTACTTCCTGCAACTTAATCCTTCAGGCAATGGCCAATACTATTAGAGACCTGTTGATATCAGA
ATAAATCGGGAGTGGTTTAA

Downstream 100 bases:

>100_bases
TTGAGTTTGTTCCCTTCCCGACCTTGTGTTAAAAACTATGATGTCGGGAAGGGTTATAAAAGTATTAATCTATTTAACTA
TGATACCAAAGGAGGAATAA

Product: ribose-5-phosphate isomerase

Products: D-ribulose 5-phosphate

Alternate protein names: NA

Number of amino acids: Translated: 158; Mature: 158

Protein sequence:

>158_residues
MKVIKNKQYVIGSDNAGYPLKEIIKELLESEGLEYEDVGVDSDQDKTIYPEIAERVVNRIKKSNYEKEGILICGTGIGMA
ITANKFSRIYAAVCHDVYSAERARLSNDTNVITMGARVIGPVLAKKIIREWLSLEFKPGRSTPKLKQIKKYENQNFVS

Sequences:

>Translated_158_residues
MKVIKNKQYVIGSDNAGYPLKEIIKELLESEGLEYEDVGVDSDQDKTIYPEIAERVVNRIKKSNYEKEGILICGTGIGMA
ITANKFSRIYAAVCHDVYSAERARLSNDTNVITMGARVIGPVLAKKIIREWLSLEFKPGRSTPKLKQIKKYENQNFVS
>Mature_158_residues
MKVIKNKQYVIGSDNAGYPLKEIIKELLESEGLEYEDVGVDSDQDKTIYPEIAERVVNRIKKSNYEKEGILICGTGIGMA
ITANKFSRIYAAVCHDVYSAERARLSNDTNVITMGARVIGPVLAKKIIREWLSLEFKPGRSTPKLKQIKKYENQNFVS

Specific function: In Addition To Its Activity On D-Ribose 5-Phosphate It Probably Also Has Activity On D-Allose 6-Phosphate. [C]

COG id: COG0698

COG function: function code G; Ribose 5-phosphate isomerase RpiB

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lacAB/rpiB family [H]

Homologues:

Organism=Escherichia coli, GI1790528, Length=149, Percent_Identity=40.9395973154362, Blast_Score=108, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003500
- InterPro:   IPR004785 [H]

Pfam domain/function: PF02502 LacAB_rpiB [H]

EC number: 5.3.1.6

Molecular weight: Translated: 17816; Mature: 17816

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVIKNKQYVIGSDNAGYPLKEIIKELLESEGLEYEDVGVDSDQDKTIYPEIAERVVNRI
CCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEECCCCCCCCCCCCCHHHHHHHHHHH
KKSNYEKEGILICGTGIGMAITANKFSRIYAAVCHDVYSAERARLSNDTNVITMGARVIG
HHCCCCCCCEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHH
PVLAKKIIREWLSLEFKPGRSTPKLKQIKKYENQNFVS
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKVIKNKQYVIGSDNAGYPLKEIIKELLESEGLEYEDVGVDSDQDKTIYPEIAERVVNRI
CCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEECCCCCCCCCCCCCHHHHHHHHHHH
KKSNYEKEGILICGTGIGMAITANKFSRIYAAVCHDVYSAERARLSNDTNVITMGARVIG
HHCCCCCCCEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHH
PVLAKKIIREWLSLEFKPGRSTPKLKQIKKYENQNFVS
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 4.4 {D-ribose} 0.83 {D-ribose} [C]

Substrates: D-ribose 5-phosphate

Specific reaction: D-ribose 5-phosphate = D-ribulose 5-phosphate

General reaction: Intramolecular oxidoreduction; Isomerization [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]