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The map label for this gene is argG [H]

Identifier: 220931138

GI number: 220931138

Start: 297665

End: 298906

Strand: Direct

Name: argG [H]

Synonym: Hore_02900

Alternate gene names: 220931138

Gene position: 297665-298906 (Clockwise)

Preceding gene: 220931137

Following gene: 220931139

Centisome position: 11.55

GC content: 42.11

Gene sequence:

>1242_bases
ATGAAGAAGGTAAATAAAATTGTTCTGGCTTATTCAGGGGGACTTGATACCTCTGTTAGTATAAAATGGTTACAGGAGAA
GTTTAATGCTGAAATAATAGCCTATTCAGCCAATGTAGGGCAGGATGATAATGACTGGAACCAGATAAAAGAGAAGGCTC
TGAAGGCTGGTGCCAGCAAGGTTTATATTGATGATATCAGGGAAGAGTTTATAAAAAACTATGCTTTTAATTCACTGAAA
GCAAATGGTTTATATGAAGGCAAATATCCCCTGGCGACTGCCCTGTCCCGGCCCCTGATTGCAAAAAGGATGGTAGAGGT
AGCCCACCAGGAAGGAGCTGAAGCTGTTGCTCATGGTTGTACTGGTAAAGGGAATGATCAGGTCAGGTTTGATGTCTCTT
TTAAAGCCCTTGATCCGGATCTGGAAATAATTGCCCCCTTGCGGGAGTGGGAATTTAAGTCCCGGGGTGAAGAAATAGAT
TATGCTAAAAAGCATAATATTCCGGTTAAAGCCACTAAGGAAAGTCCTTACAGTATCGATAGTAACCTCTGGGGGTTAAG
TGTTGAATGTGGGGAGTTGGAAGATCCGTGGGCAGAACCTCCACTTGACGCCTATCAGTGGACGGCAAATCCCGAAGAAG
CCCCTGCTACCCCCCTCTATTTAACAATATCATTTGACCAGGGGGAACCCATTAAATTAAATGACAGGTATATGTCCCCT
GTTGAATTGGTGGAAAAATTAAATGATATCGGGGCCGAATATGGTATAGGGAGAATAGATATGGTGGAAAACAGACTGGT
CGGTATCAAATCCAGGGAGATCTATGAGACCCCGGCTGCCACAATTTTACTTGAAGCTCATCAGCAGCTTGAAGACTTAA
CTCTGGATAGGGAAACAGCCCATTACAAAAGATTGATAAGTGAAAAGTATTCCGAACTGGTTTATAACGGGTTATGGTAT
TCACCATTACGGTCAGCCCTTGAAGCCTTTATTGAAGATACCCAGAAACAGGTTACCGGTAAAGTTAAACTAAAGCTTTA
CAAGGGTCAGTGTGTTACGGTTGGTCGAAAGTCTCCCTATTCGCTCTACCAGTTTGATCTTGCGACGTATGATGAGAATG
ATTCTTTTGACCATTCATCGGCAAAAGGATTTATCAAGCTATGGGGTTTACCAGGTCAGGTATATAGTCAGGTTCAGCAG
AAAAATAAAGATACTATTTATCTAAAGGAGTTTGTAAAATGA

Upstream 100 bases:

>100_bases
ATACTGTAGAGATGGTGTTACAGGCTCTGGTAAGGGTAGGAGAAGAGGAGAAGAAAGAGGTCATACCGTTATCTGAATAT
AAAAAAGGAGGAGTTTTAGT

Downstream 100 bases:

>100_bases
GGTCCCTGTCCTCTCCTGATAACAGTGTCTGTATCAGGGGAGCTAATACAGAAGATGCCGGTAAGGTACTGATACTGATC
AGGCGTGCCTTTAAGGATTA

Product: Argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase [H]

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MKKVNKIVLAYSGGLDTSVSIKWLQEKFNAEIIAYSANVGQDDNDWNQIKEKALKAGASKVYIDDIREEFIKNYAFNSLK
ANGLYEGKYPLATALSRPLIAKRMVEVAHQEGAEAVAHGCTGKGNDQVRFDVSFKALDPDLEIIAPLREWEFKSRGEEID
YAKKHNIPVKATKESPYSIDSNLWGLSVECGELEDPWAEPPLDAYQWTANPEEAPATPLYLTISFDQGEPIKLNDRYMSP
VELVEKLNDIGAEYGIGRIDMVENRLVGIKSREIYETPAATILLEAHQQLEDLTLDRETAHYKRLISEKYSELVYNGLWY
SPLRSALEAFIEDTQKQVTGKVKLKLYKGQCVTVGRKSPYSLYQFDLATYDENDSFDHSSAKGFIKLWGLPGQVYSQVQQ
KNKDTIYLKEFVK

Sequences:

>Translated_413_residues
MKKVNKIVLAYSGGLDTSVSIKWLQEKFNAEIIAYSANVGQDDNDWNQIKEKALKAGASKVYIDDIREEFIKNYAFNSLK
ANGLYEGKYPLATALSRPLIAKRMVEVAHQEGAEAVAHGCTGKGNDQVRFDVSFKALDPDLEIIAPLREWEFKSRGEEID
YAKKHNIPVKATKESPYSIDSNLWGLSVECGELEDPWAEPPLDAYQWTANPEEAPATPLYLTISFDQGEPIKLNDRYMSP
VELVEKLNDIGAEYGIGRIDMVENRLVGIKSREIYETPAATILLEAHQQLEDLTLDRETAHYKRLISEKYSELVYNGLWY
SPLRSALEAFIEDTQKQVTGKVKLKLYKGQCVTVGRKSPYSLYQFDLATYDENDSFDHSSAKGFIKLWGLPGQVYSQVQQ
KNKDTIYLKEFVK
>Mature_413_residues
MKKVNKIVLAYSGGLDTSVSIKWLQEKFNAEIIAYSANVGQDDNDWNQIKEKALKAGASKVYIDDIREEFIKNYAFNSLK
ANGLYEGKYPLATALSRPLIAKRMVEVAHQEGAEAVAHGCTGKGNDQVRFDVSFKALDPDLEIIAPLREWEFKSRGEEID
YAKKHNIPVKATKESPYSIDSNLWGLSVECGELEDPWAEPPLDAYQWTANPEEAPATPLYLTISFDQGEPIKLNDRYMSP
VELVEKLNDIGAEYGIGRIDMVENRLVGIKSREIYETPAATILLEAHQQLEDLTLDRETAHYKRLISEKYSELVYNGLWY
SPLRSALEAFIEDTQKQVTGKVKLKLYKGQCVTVGRKSPYSLYQFDLATYDENDSFDHSSAKGFIKLWGLPGQVYSQVQQ
KNKDTIYLKEFVK

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI53759107, Length=410, Percent_Identity=42.9268292682927, Blast_Score=331, Evalue=7e-91,
Organism=Homo sapiens, GI16950633, Length=410, Percent_Identity=42.9268292682927, Blast_Score=331, Evalue=7e-91,
Organism=Escherichia coli, GI1789563, Length=373, Percent_Identity=30.5630026809651, Blast_Score=142, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6324514, Length=418, Percent_Identity=40.4306220095694, Blast_Score=306, Evalue=3e-84,
Organism=Drosophila melanogaster, GI21358151, Length=394, Percent_Identity=42.8934010152284, Blast_Score=317, Evalue=7e-87,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00764 Arginosuc_synth [H]

EC number: =6.3.4.5 [H]

Molecular weight: Translated: 46822; Mature: 46822

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1 ; PS00565 ARGININOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVNKIVLAYSGGLDTSVSIKWLQEKFNAEIIAYSANVGQDDNDWNQIKEKALKAGASK
CCCCCEEEEEEECCCCCCCHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCE
VYIDDIREEFIKNYAFNSLKANGLYEGKYPLATALSRPLIAKRMVEVAHQEGAEAVAHGC
EEHHHHHHHHHHHHHCCCCCCCCEECCCCCHHHHHHCHHHHHHHHHHHHHCCHHHHHHCC
TGKGNDQVRFDVSFKALDPDLEIIAPLREWEFKSRGEEIDYAKKHNIPVKATKESPYSID
CCCCCCEEEEEEEEEECCCCHHEECCHHHHHHHCCCCCCCCHHHCCCCEEECCCCCCCCC
SNLWGLSVECGELEDPWAEPPLDAYQWTANPEEAPATPLYLTISFDQGEPIKLNDRYMSP
CCCEEEEEECCCCCCCCCCCCCCCEEECCCCCCCCCCEEEEEEEECCCCCEEECCCCCCH
VELVEKLNDIGAEYGIGRIDMVENRLVGIKSREIYETPAATILLEAHQQLEDLTLDRETA
HHHHHHHHHHHHHCCCCCCEEHHCCEECCCCCHHHCCCHHHHHHHHHHHHHHHCCCHHHH
HYKRLISEKYSELVYNGLWYSPLRSALEAFIEDTQKQVTGKVKLKLYKGQCVTVGRKSPY
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEEEECCCCCC
SLYQFDLATYDENDSFDHSSAKGFIKLWGLPGQVYSQVQQKNKDTIYLKEFVK
EEEEEEEEECCCCCCCCCCCCCCEEEEECCCHHHHHHHHHCCCCEEEHHHHCC
>Mature Secondary Structure
MKKVNKIVLAYSGGLDTSVSIKWLQEKFNAEIIAYSANVGQDDNDWNQIKEKALKAGASK
CCCCCEEEEEEECCCCCCCHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCE
VYIDDIREEFIKNYAFNSLKANGLYEGKYPLATALSRPLIAKRMVEVAHQEGAEAVAHGC
EEHHHHHHHHHHHHHCCCCCCCCEECCCCCHHHHHHCHHHHHHHHHHHHHCCHHHHHHCC
TGKGNDQVRFDVSFKALDPDLEIIAPLREWEFKSRGEEIDYAKKHNIPVKATKESPYSID
CCCCCCEEEEEEEEEECCCCHHEECCHHHHHHHCCCCCCCCHHHCCCCEEECCCCCCCCC
SNLWGLSVECGELEDPWAEPPLDAYQWTANPEEAPATPLYLTISFDQGEPIKLNDRYMSP
CCCEEEEEECCCCCCCCCCCCCCCEEECCCCCCCCCCEEEEEEEECCCCCEEECCCCCCH
VELVEKLNDIGAEYGIGRIDMVENRLVGIKSREIYETPAATILLEAHQQLEDLTLDRETA
HHHHHHHHHHHHHCCCCCCEEHHCCEECCCCCHHHCCCHHHHHHHHHHHHHHHCCCHHHH
HYKRLISEKYSELVYNGLWYSPLRSALEAFIEDTQKQVTGKVKLKLYKGQCVTVGRKSPY
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEEEECCCCCC
SLYQFDLATYDENDSFDHSSAKGFIKLWGLPGQVYSQVQQKNKDTIYLKEFVK
EEEEEEEEECCCCCCCCCCCCCCEEEEECCCHHHHHHHHHCCCCEEEHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA