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The map label for this gene is hisZ

Identifier: 220931066

GI number: 220931066

Start: 225743

End: 226999

Strand: Direct

Name: hisZ

Synonym: Hore_02180

Alternate gene names: 220931066

Gene position: 225743-226999 (Clockwise)

Preceding gene: 220931065

Following gene: 220931067

Centisome position: 8.76

GC content: 39.06

Gene sequence:

>1257_bases
ATGACAGAGAATATTTTAAATTCACCGGGGGGAATGAGGAGTTATCTTGATTCAATAGCATTTCAACTGGAAGAGATCCA
GGATAGAATAAAAGGTGTATTTAGACAGTGGGCCTATAGACCAATAATTACCCCTACTCTGGAATATTATGAGTCCCTAA
CTACCGGTATCGGCGAGAAATATAAAAAACAGATGTATAAGTTTATAGATTATGAAGGTAATATTTTAGCCCTGAGACCG
GAGATGACGGCTCCTATTGCCAGGACGGTAGCCAACAAAATAGATGAGCTGTGCCTGCCACAACGCTTATCATACCGGGC
TCCGGTTTTCAGATACGAAGAACCACAGACCGGGAAAAACCGGGAAATATACCAGATAGGGGTTGAATTAATCGGAGAAA
AGAGCCCGGGAGCTGATGCTGAAGTTATCATGCTGGCTGTTGAATCCCTTAAAAGTAGTGGTTTAACTGATTTTCAGATT
GATATAGGCCATGCCGGGTTTTTAAATGGGGTTATTGAAGAATTAAAGGTCACAGATAGTCAGGGGGAACAGATTAAACG
ATGGTTAAATAAAAAGGATATGGTCAGTATAAGGGATTTTACCAGTAGAGTGGAGATTAAAAATATTAATAAATTACTGG
GAATAGTCCGTCTGAGAGGCAAAAAAGAGGTGCTGCAAAGGGCAAAAAGACTGATCAATAATGATAAATCAAAAAAAGCT
CTAAAGGATTTAGAACTGGTCTATGAATACCTGTGCGATTATGGAGTAGATAATTATGTTAATTTTGATCTGACCCTGAT
CAGGGGTTTTGAATATTATACCGGTATTGTCTTTGAAGCCTTTACCGAAAATCTTGGTTATACCATCTGTGGGGGAGGCA
GGTATGATAGCTTAATATACCAGTATTGTGGAAAAGAGATTCCGGCTATTGGTTTTGCCATTGGAATTGAAAGGGTCAGG
CTGGGTCTTTTGAATCAGGGTCAGGAATTGGAGACACCAGAGATTGATGTTATGGTTGTTTTTTCATATCAGGCCAGAAA
ACCAGCCCTTGAGGCGATAAAAAAATACAGGAAACAGGGGTTAAATGTTCTTCAGATTGAAAAAGAAGAGGTGGATCAAG
AATTTATTAAAAAACACCTGAAAACAGGGGTTAAAAAAATTATTTCATTCTGTGAATACTCCAGTAACCAGAAAATTAAA
GTTATTGATGACAGGGGAAACATCGAGCTATTGACACCCGGGGGTGATTTGCCATGA

Upstream 100 bases:

>100_bases
GCTGAAAATCAAAAACTCCTTGACAGGGGATGTTATTTATGTTATATTCTTTATTAAACTAAAGTAATAAAATATTAAAG
TATTAAAGGATGATATAAAA

Downstream 100 bases:

>100_bases
AATCAGTTATTACAGCCCTTCCCAAGGGAAGATTGATGGGAGAGGTTATAGATATCCTTAAACAAAGTGGGTTTATATCC
AATAATATTGATATTAATAC

Product: histidyl-tRNA synthetase

Products: AMP; diphosphate +L-histidyl-tRNAHis [C]

Alternate protein names: NA

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MTENILNSPGGMRSYLDSIAFQLEEIQDRIKGVFRQWAYRPIITPTLEYYESLTTGIGEKYKKQMYKFIDYEGNILALRP
EMTAPIARTVANKIDELCLPQRLSYRAPVFRYEEPQTGKNREIYQIGVELIGEKSPGADAEVIMLAVESLKSSGLTDFQI
DIGHAGFLNGVIEELKVTDSQGEQIKRWLNKKDMVSIRDFTSRVEIKNINKLLGIVRLRGKKEVLQRAKRLINNDKSKKA
LKDLELVYEYLCDYGVDNYVNFDLTLIRGFEYYTGIVFEAFTENLGYTICGGGRYDSLIYQYCGKEIPAIGFAIGIERVR
LGLLNQGQELETPEIDVMVVFSYQARKPALEAIKKYRKQGLNVLQIEKEEVDQEFIKKHLKTGVKKIISFCEYSSNQKIK
VIDDRGNIELLTPGGDLP

Sequences:

>Translated_418_residues
MTENILNSPGGMRSYLDSIAFQLEEIQDRIKGVFRQWAYRPIITPTLEYYESLTTGIGEKYKKQMYKFIDYEGNILALRP
EMTAPIARTVANKIDELCLPQRLSYRAPVFRYEEPQTGKNREIYQIGVELIGEKSPGADAEVIMLAVESLKSSGLTDFQI
DIGHAGFLNGVIEELKVTDSQGEQIKRWLNKKDMVSIRDFTSRVEIKNINKLLGIVRLRGKKEVLQRAKRLINNDKSKKA
LKDLELVYEYLCDYGVDNYVNFDLTLIRGFEYYTGIVFEAFTENLGYTICGGGRYDSLIYQYCGKEIPAIGFAIGIERVR
LGLLNQGQELETPEIDVMVVFSYQARKPALEAIKKYRKQGLNVLQIEKEEVDQEFIKKHLKTGVKKIISFCEYSSNQKIK
VIDDRGNIELLTPGGDLP
>Mature_417_residues
TENILNSPGGMRSYLDSIAFQLEEIQDRIKGVFRQWAYRPIITPTLEYYESLTTGIGEKYKKQMYKFIDYEGNILALRPE
MTAPIARTVANKIDELCLPQRLSYRAPVFRYEEPQTGKNREIYQIGVELIGEKSPGADAEVIMLAVESLKSSGLTDFQID
IGHAGFLNGVIEELKVTDSQGEQIKRWLNKKDMVSIRDFTSRVEIKNINKLLGIVRLRGKKEVLQRAKRLINNDKSKKAL
KDLELVYEYLCDYGVDNYVNFDLTLIRGFEYYTGIVFEAFTENLGYTICGGGRYDSLIYQYCGKEIPAIGFAIGIERVRL
GLLNQGQELETPEIDVMVVFSYQARKPALEAIKKYRKQGLNVLQIEKEEVDQEFIKKHLKTGVKKIISFCEYSSNQKIKV
IDDRGNIELLTPGGDLP

Specific function: Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine

COG id: COG3705

COG function: function code E; ATP phosphoribosyltransferase involved in histidine biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily

Homologues:

Organism=Homo sapiens, GI6996014, Length=381, Percent_Identity=30.4461942257218, Blast_Score=138, Evalue=9e-33,
Organism=Homo sapiens, GI15029520, Length=366, Percent_Identity=28.1420765027322, Blast_Score=136, Evalue=4e-32,
Organism=Escherichia coli, GI1788861, Length=420, Percent_Identity=28.5714285714286, Blast_Score=129, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI71993693, Length=357, Percent_Identity=28.0112044817927, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI71993686, Length=357, Percent_Identity=28.0112044817927, Blast_Score=96, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6325290, Length=358, Percent_Identity=28.4916201117318, Blast_Score=131, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24643061, Length=384, Percent_Identity=28.3854166666667, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24643059, Length=403, Percent_Identity=27.0471464019851, Blast_Score=118, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HISZ_HALOH (B8D110)

Other databases:

- EMBL:   CP001098
- RefSeq:   YP_002507974.1
- ProteinModelPortal:   B8D110
- SMR:   B8D110
- GeneID:   7312538
- GenomeReviews:   CP001098_GR
- KEGG:   hor:Hore_02180
- HOGENOM:   HBG616575
- OMA:   ANLIREC
- GO:   GO:0005737
- HAMAP:   MF_00125
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004517
- InterPro:   IPR004516
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00443

Pfam domain/function: PF00587 tRNA-synt_2b

EC number: 6.1.1.21 [C]

Molecular weight: Translated: 47802; Mature: 47671

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTENILNSPGGMRSYLDSIAFQLEEIQDRIKGVFRQWAYRPIITPTLEYYESLTTGIGEK
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
YKKQMYKFIDYEGNILALRPEMTAPIARTVANKIDELCLPQRLSYRAPVFRYEEPQTGKN
HHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCHHHCCCCCCEECCCCCCCCC
REIYQIGVELIGEKSPGADAEVIMLAVESLKSSGLTDFQIDIGHAGFLNGVIEELKVTDS
CHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCC
QGEQIKRWLNKKDMVSIRDFTSRVEIKNINKLLGIVRLRGKKEVLQRAKRLINNDKSKKA
CHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHH
LKDLELVYEYLCDYGVDNYVNFDLTLIRGFEYYTGIVFEAFTENLGYTICGGGRYDSLIY
HHHHHHHHHHHHHCCCCCEECCEEEEEECHHHHHHHHHHHHHHCCCEEEECCCHHHHHHH
QYCGKEIPAIGFAIGIERVRLGLLNQGQELETPEIDVMVVFSYQARKPALEAIKKYRKQG
HHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHCC
LNVLQIEKEEVDQEFIKKHLKTGVKKIISFCEYSSNQKIKVIDDRGNIELLTPGGDLP
CCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEECCCCCCC
>Mature Secondary Structure 
TENILNSPGGMRSYLDSIAFQLEEIQDRIKGVFRQWAYRPIITPTLEYYESLTTGIGEK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
YKKQMYKFIDYEGNILALRPEMTAPIARTVANKIDELCLPQRLSYRAPVFRYEEPQTGKN
HHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCHHHCCCCCCEECCCCCCCCC
REIYQIGVELIGEKSPGADAEVIMLAVESLKSSGLTDFQIDIGHAGFLNGVIEELKVTDS
CHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCC
QGEQIKRWLNKKDMVSIRDFTSRVEIKNINKLLGIVRLRGKKEVLQRAKRLINNDKSKKA
CHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHH
LKDLELVYEYLCDYGVDNYVNFDLTLIRGFEYYTGIVFEAFTENLGYTICGGGRYDSLIY
HHHHHHHHHHHHHCCCCCEECCEEEEEECHHHHHHHHHHHHHHCCCEEEECCCHHHHHHH
QYCGKEIPAIGFAIGIERVRLGLLNQGQELETPEIDVMVVFSYQARKPALEAIKKYRKQG
HHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHCC
LNVLQIEKEEVDQEFIKKHLKTGVKKIISFCEYSSNQKIKVIDDRGNIELLTPGGDLP
CCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): 8520 [C]

Specific activity: 121.8

Km value (mM): 0.06 {His}} 0.046 {His}} 0.03 {His}} 0.02 {His}} 0.017 {His}} 0.011 {His}} 0.008 {His}} 0.0014 {tRNAHis}} 0.00056 {tRNAHis}} 0.0016 {U73tRNAHisGUG}} 0.00031 {U73tRNAHisGUG}} 0.89 {ATP}} 0.74 {ATP}} 0.655 {ATP}} 0.5

Substrates: ATP; @HIST01.txt*L-histidine!; tRNAHis [C]

Specific reaction: ATP + @HIST01.txt*L-histidine! + tRNAHis = AMP + diphosphate +L-histidyl-tRNAHis [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 1, 2, 4-Triazole-3-alanine; D-Histidine; Diphosphate; L-Histidinol; Mg2+; N-Acetyl histidine; NEM; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA