Click here to switch to the map view.

The map label for this gene is ispF

Identifier: 220930941

GI number: 220930941

Start: 111733

End: 112230

Strand: Direct

Name: ispF

Synonym: Hore_00930

Alternate gene names: 220930941

Gene position: 111733-112230 (Clockwise)

Preceding gene: 220930940

Following gene: 220930942

Centisome position: 4.33

GC content: 37.95

Gene sequence:

>498_bases
ATGAGGATTGGGATTGGATTTGATGTTCATCCCCTGAAAAAAGGGGAAACCTTGATTCTAGGTGGTGTACATATACAGGG
TGAATATGGTCTTGCCGGTCATTCAGATGCTGATGTATTAACCCATGCCCTGATGGATGCTATTCTGGGGGCGATGGGGG
AAGCTGATATAGGTTATCATTTTCCCGACACTGATCCTTCATATAGGGGGATCAGTAGTTTAAAATTGTTAAAAAAAGTA
TATAATATGATGAAACCCAGGGGTTTTTCTATTGGGAATATTGACCTTATTATTATGGCCCAATCGCCAAAGTTGTCACC
TTATTATAATAAGATAAAAGAAAATTATACCCGAATATTACAGTTAGATAGTTCCAGAATAAATATAAAAGCGACTACCA
CTGAAAATTTAGGTTTTGTAGGCAGGGAAGAAGGGATTGCTGCTCAGGCTGCTGTGTTATTAATTGATACCAAGGAAGGT
GATATTAATGCTGGTTAA

Upstream 100 bases:

>100_bases
CCAGTTAAGGTTATCAAAGGTTCTTATGATAATATTAAAGTAACAACACCGGTTGATTTAATTATTGCTGAAGCAATACT
AAAAACAGGAGACTAGAACT

Downstream 100 bases:

>100_bases
AAATATAATGTCAAAAGACCCGATAACTATTTCTCCTGAAACTACAGTTGTAGAAGCAGAAAAATTAATGTCTATCAATA
ACATCGGGCGGTTAATAGTT

Product: 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 165; Mature: 165

Protein sequence:

>165_residues
MRIGIGFDVHPLKKGETLILGGVHIQGEYGLAGHSDADVLTHALMDAILGAMGEADIGYHFPDTDPSYRGISSLKLLKKV
YNMMKPRGFSIGNIDLIIMAQSPKLSPYYNKIKENYTRILQLDSSRINIKATTTENLGFVGREEGIAAQAAVLLIDTKEG
DINAG

Sequences:

>Translated_165_residues
MRIGIGFDVHPLKKGETLILGGVHIQGEYGLAGHSDADVLTHALMDAILGAMGEADIGYHFPDTDPSYRGISSLKLLKKV
YNMMKPRGFSIGNIDLIIMAQSPKLSPYYNKIKENYTRILQLDSSRINIKATTTENLGFVGREEGIAAQAAVLLIDTKEG
DINAG
>Mature_165_residues
MRIGIGFDVHPLKKGETLILGGVHIQGEYGLAGHSDADVLTHALMDAILGAMGEADIGYHFPDTDPSYRGISSLKLLKKV
YNMMKPRGFSIGNIDLIIMAQSPKLSPYYNKIKENYTRILQLDSSRINIKATTTENLGFVGREEGIAAQAAVLLIDTKEG
DINAG

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=155, Percent_Identity=47.741935483871, Blast_Score=143, Evalue=7e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_HALOH (B8D0A0)

Other databases:

- EMBL:   CP001098
- RefSeq:   YP_002507849.1
- GeneID:   7314315
- GenomeReviews:   CP001098_GR
- KEGG:   hor:Hore_00930
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 17918; Mature: 17918

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGIGFDVHPLKKGETLILGGVHIQGEYGLAGHSDADVLTHALMDAILGAMGEADIGYH
CEECCCCCCCCCCCCCEEEEECEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
FPDTDPSYRGISSLKLLKKVYNMMKPRGFSIGNIDLIIMAQSPKLSPYYNKIKENYTRIL
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHEEE
QLDSSRINIKATTTENLGFVGREEGIAAQAAVLLIDTKEGDINAG
EECCCEEEEEEECCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCC
>Mature Secondary Structure
MRIGIGFDVHPLKKGETLILGGVHIQGEYGLAGHSDADVLTHALMDAILGAMGEADIGYH
CEECCCCCCCCCCCCCEEEEECEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
FPDTDPSYRGISSLKLLKKVYNMMKPRGFSIGNIDLIIMAQSPKLSPYYNKIKENYTRIL
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHEEE
QLDSSRINIKATTTENLGFVGREEGIAAQAAVLLIDTKEGDINAG
EECCCEEEEEEECCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA