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The map label for this gene is birA [H]

Identifier: 220930914

GI number: 220930914

Start: 77808

End: 78788

Strand: Direct

Name: birA [H]

Synonym: Hore_00660

Alternate gene names: 220930914

Gene position: 77808-78788 (Clockwise)

Preceding gene: 220930912

Following gene: 220930915

Centisome position: 3.02

GC content: 38.12

Gene sequence:

>981_bases
ATGGAATTGAATAAAAAAGAAATAGAACTGGTTAAAATATTACATAATAATAATGGTAAATTTATCTCCGGTGAAAATAT
AAGTGATACCCTTGGAGTTTCACGTACTGCCGTCTGGAAATATATAAAAAATCTGCGGAAAAAGGGTTATATTATAGAAT
CAGTTCCCAGGCGTGGTTATCATCTGGTTGAGGTTCCCGATAAAATTACAGTAACTGAAGTCCTCGCCGGCCTGAAAACA
AACCTGATTGGTAAAGAGGTCAGGGTTTTTTCAAGTATAGATTCAACAAATAATAAAGCACGAGAACTGGCCAAAGGGGG
TGCCAGTGGAGGAACAATTGTTATTGCTGAAGAACAGACAGGTGGCAAGGGGAGACTGGGGAGGAAATGGTATTCCCCAC
CATATACCGGTCTCTGGTTTTCTGTAATAGTCAGGCCCCATCTTCCTTCAAACAGGGCTCCATTTTTAACTATTATAACC
TCACTGGTAGTTAAAAAAGCAATTAACAAACTGGGGATTGAAGTTTTAATAAAATGGCCCAATGATATTTTAATCAGGGG
TAAAAAGGTCTGTGGTATTCTATCTGAGATTATGGCAACTCCTGATGTAATCAATACAGCTATAATCGGGATAGGTATTA
ATGTAAACCAGGATAGTTTTCCTTCAAAGATACCTCTGGCGACTTCTCTTAAACAGGTCTGTGGCCATAACGTAAACCGT
ATTGAACTACTTCAGGATATTTTAATGGAGTTTGAAAATTACTATAATAGACTTAAAGATGGATGTTATCGGGACCTGGT
TACAGAGTGGAAAAATTCAATGGATATTCTGGGTAAAGAGGTTGATATAGTGGCCGGTAATGAGATTATTTCTGGTAAAG
TGGTTTCAATATCCAGGAGAGGAGAACTGGTTTTAATAGACCCTGATGGGAATAAACATTTATTCTGGGCCGGGGATGTA
ACCCTCAGGCAAAAAGATTAA

Upstream 100 bases:

>100_bases
ATTTGTTTGCAACACTAAAAGGTTAGCTTAAATGTCGATTATTATAAATTTTTCATATGAAGTTTGCTTATGATAAAGAT
ATTAAAAGAAAAGGGGGGGA

Downstream 100 bases:

>100_bases
ATAATTCATTTTTTGAGCAGGAAATTTTTTTTTAATGAAGAAATTTATATTTAGGACAGGCCGGTTAGACAGGTCAGACC
TTTTGGATGGTCTTACATAT

Product: biotin (acetyl-CoA carboxylase) synthetase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MELNKKEIELVKILHNNNGKFISGENISDTLGVSRTAVWKYIKNLRKKGYIIESVPRRGYHLVEVPDKITVTEVLAGLKT
NLIGKEVRVFSSIDSTNNKARELAKGGASGGTIVIAEEQTGGKGRLGRKWYSPPYTGLWFSVIVRPHLPSNRAPFLTIIT
SLVVKKAINKLGIEVLIKWPNDILIRGKKVCGILSEIMATPDVINTAIIGIGINVNQDSFPSKIPLATSLKQVCGHNVNR
IELLQDILMEFENYYNRLKDGCYRDLVTEWKNSMDILGKEVDIVAGNEIISGKVVSISRRGELVLIDPDGNKHLFWAGDV
TLRQKD

Sequences:

>Translated_326_residues
MELNKKEIELVKILHNNNGKFISGENISDTLGVSRTAVWKYIKNLRKKGYIIESVPRRGYHLVEVPDKITVTEVLAGLKT
NLIGKEVRVFSSIDSTNNKARELAKGGASGGTIVIAEEQTGGKGRLGRKWYSPPYTGLWFSVIVRPHLPSNRAPFLTIIT
SLVVKKAINKLGIEVLIKWPNDILIRGKKVCGILSEIMATPDVINTAIIGIGINVNQDSFPSKIPLATSLKQVCGHNVNR
IELLQDILMEFENYYNRLKDGCYRDLVTEWKNSMDILGKEVDIVAGNEIISGKVVSISRRGELVLIDPDGNKHLFWAGDV
TLRQKD
>Mature_326_residues
MELNKKEIELVKILHNNNGKFISGENISDTLGVSRTAVWKYIKNLRKKGYIIESVPRRGYHLVEVPDKITVTEVLAGLKT
NLIGKEVRVFSSIDSTNNKARELAKGGASGGTIVIAEEQTGGKGRLGRKWYSPPYTGLWFSVIVRPHLPSNRAPFLTIIT
SLVVKKAINKLGIEVLIKWPNDILIRGKKVCGILSEIMATPDVINTAIIGIGINVNQDSFPSKIPLATSLKQVCGHNVNR
IELLQDILMEFENYYNRLKDGCYRDLVTEWKNSMDILGKEVDIVAGNEIISGKVVSISRRGELVLIDPDGNKHLFWAGDV
TLRQKD

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=332, Percent_Identity=29.8192771084337, Blast_Score=133, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 36284; Mature: 36284

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELNKKEIELVKILHNNNGKFISGENISDTLGVSRTAVWKYIKNLRKKGYIIESVPRRGY
CCCCHHHHHEEEEEECCCCCEEECCCCCHHHCCHHHHHHHHHHHHHHCCCEEECCCCCCE
HLVEVPDKITVTEVLAGLKTNLIGKEVRVFSSIDSTNNKARELAKGGASGGTIVIAEEQT
EEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCEEEEEECCC
GGKGRLGRKWYSPPYTGLWFSVIVRPHLPSNRAPFLTIITSLVVKKAINKLGIEVLIKWP
CCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEECC
NDILIRGKKVCGILSEIMATPDVINTAIIGIGINVNQDSFPSKIPLATSLKQVCGHNVNR
CCEEEECHHHHHHHHHHHCCCHHHHHEEEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCH
IELLQDILMEFENYYNRLKDGCYRDLVTEWKNSMDILGKEVDIVAGNEIISGKVVSISRR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEEECCEEEEEECC
GELVLIDPDGNKHLFWAGDVTLRQKD
CCEEEECCCCCEEEEEECCEEEECCC
>Mature Secondary Structure
MELNKKEIELVKILHNNNGKFISGENISDTLGVSRTAVWKYIKNLRKKGYIIESVPRRGY
CCCCHHHHHEEEEEECCCCCEEECCCCCHHHCCHHHHHHHHHHHHHHCCCEEECCCCCCE
HLVEVPDKITVTEVLAGLKTNLIGKEVRVFSSIDSTNNKARELAKGGASGGTIVIAEEQT
EEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCEEEEEECCC
GGKGRLGRKWYSPPYTGLWFSVIVRPHLPSNRAPFLTIITSLVVKKAINKLGIEVLIKWP
CCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEECC
NDILIRGKKVCGILSEIMATPDVINTAIIGIGINVNQDSFPSKIPLATSLKQVCGHNVNR
CCEEEECHHHHHHHHHHHCCCHHHHHEEEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCH
IELLQDILMEFENYYNRLKDGCYRDLVTEWKNSMDILGKEVDIVAGNEIISGKVVSISRR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEEECCEEEEEECC
GELVLIDPDGNKHLFWAGDVTLRQKD
CCEEEECCCCCEEEEEECCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]