Click here to switch to the map view.

The map label for this gene is nadB [H]

Identifier: 220930911

GI number: 220930911

Start: 73468

End: 75066

Strand: Direct

Name: nadB [H]

Synonym: Hore_00630

Alternate gene names: 220930911

Gene position: 73468-75066 (Clockwise)

Preceding gene: 220930910

Following gene: 220930912

Centisome position: 2.85

GC content: 42.15

Gene sequence:

>1599_bases
ATGGGAGATATAAAATATAAAACTACAGATTTTGTTATAATCGGGACTGGAATAGCTGGTCTGTATACTGCCCTCAAACT
GGCCAGTCTGGGTGAGGTTTCTGTTTTAACCAAGGAAAAACTGGAGGATAGTAACACTCAGTATGCCCAGGGGGGTATTG
CTGCAGTTATAGACAGGGGTGATTCCTGGAAACTTCATATGGAAGATACCCTTAAAGCGGGGGCCGGAATATGTGATGAA
AAGGCGGTAGAAGTGTTAGTTACTGAAGGTCCAGACCGGGTCCGGGAATTAATTAAGATGGGTACAAGATTTGATCATAT
AGAAGGGGAACTTGATTTAACCAGAGAAGGAGCCCATAGCAAGAGGCGAATTCTCCATGCCCGGGGGGATGCAACCGGTG
AAGAAATTCGGGAATCCCTGACCCGGGCTATAACTAAATCCAAAAATATTTCCCTTAAAGAAGAAAATTTTATGATTGAC
CTGGTTAAATTAAACCGGGGCAACCATGTGGATGGAGTCCTGGTCTGGGATAATAATCAAAAAAAATATATTGTTTACCT
GGCCAGAGCTGTAATTCTGGCAAGTGGTGGTTGTGGACAGGTCTACCATAATACCAGTAACCCTGAGGTAACCACCGGGG
ATGGAGTGGCAGCTGCCTACAGGGCAGGGGCCATAATAATGGATATGGAATTTATCCAGTTTCACCCCACTGTTTTTTAT
AATAAACACGGTTCTTCTTTTTTAATCTCAGAAAGCTTACGGGGTGAAGGGGCTGTTCTTAGAAACAGTGAAGGTAAACG
CTTTATGGATAAATACCATGACCTGGCTGAACTGGCCCCAAGGGATGTAGTAGCCCGGGCAATTTTGCAGGAAATGGAAA
GAGATAATAAACCTCATGTGTGGCTGGATATAACCCATAAAGATGCCGATTTTGTTAAAAACAGATTTCCAACTATATTT
AGAACCCTTAAAGAGCATGGTATAGATATAACCAGGGACTGGGTTCCGGTGGTTCCTGCAGCCCATTATATGATGGGAGG
AGTAAGGACTGATACTTATGGTAGAACAAATTTAATTGGTCTTTATGCAGTCGGGGAAGTTGCCTGTACCGGTGTTCATG
GTGCCAACAGACTGGCGAGCAATTCTCTTCTGGAAGGACTTGTTTTTGGTAATAGAATATACAGGGCCCTGAAAATGGAA
GCCGATGTCCTGCCCCTACCAGAAGAAAATCTCAAGGTTCCTCAATTATTACCTTATGGAAAGGTAATGGTCATAAAACA
GATAAAAGAAGAGTTGAAAAAGAAGATGTCCCATCAGGTAGGTATTATCAGGAGTGAGAAAACCTTAAAAGATATGATAA
AGTGGATAGAAGAGACAGAGGTTAAGCTTAATAAATTTAAATATAGTAATGTTGAGTTATGGGAGCTAAAGAATATGCTT
TCAGTAGCTGGAATTATTTCCAGGAGTGCCCTGTTAAGAAAAGAAAGCCGGGGTGGGCATTTCAGGGAAGATTATCCGGA
AATAAAACCTGGCTGGGGGAATAAACATGTCCTGGTAGATAAACTTAGTCCGGAGGGGAGGATTCATGAAGTTAAATAA

Upstream 100 bases:

>100_bases
GGTAGAAATACCGGAAAACATAAGAACCAGGGCCTATAATGCCTTGAAAAAAATGCTGGAGGTCAAATAACCTCACTAAA
TGACGGTAGGGTGGATAAAC

Downstream 100 bases:

>100_bases
TATTTATCTGGAGAAAGTCATTATTGAAGCCCTTGAAGAGGATATAGGGTTTGGCGATTTAACAACAGATTATTTAATAC
CATCCACTCACAGGTCCTCC

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 532; Mature: 531

Protein sequence:

>532_residues
MGDIKYKTTDFVIIGTGIAGLYTALKLASLGEVSVLTKEKLEDSNTQYAQGGIAAVIDRGDSWKLHMEDTLKAGAGICDE
KAVEVLVTEGPDRVRELIKMGTRFDHIEGELDLTREGAHSKRRILHARGDATGEEIRESLTRAITKSKNISLKEENFMID
LVKLNRGNHVDGVLVWDNNQKKYIVYLARAVILASGGCGQVYHNTSNPEVTTGDGVAAAYRAGAIIMDMEFIQFHPTVFY
NKHGSSFLISESLRGEGAVLRNSEGKRFMDKYHDLAELAPRDVVARAILQEMERDNKPHVWLDITHKDADFVKNRFPTIF
RTLKEHGIDITRDWVPVVPAAHYMMGGVRTDTYGRTNLIGLYAVGEVACTGVHGANRLASNSLLEGLVFGNRIYRALKME
ADVLPLPEENLKVPQLLPYGKVMVIKQIKEELKKKMSHQVGIIRSEKTLKDMIKWIEETEVKLNKFKYSNVELWELKNML
SVAGIISRSALLRKESRGGHFREDYPEIKPGWGNKHVLVDKLSPEGRIHEVK

Sequences:

>Translated_532_residues
MGDIKYKTTDFVIIGTGIAGLYTALKLASLGEVSVLTKEKLEDSNTQYAQGGIAAVIDRGDSWKLHMEDTLKAGAGICDE
KAVEVLVTEGPDRVRELIKMGTRFDHIEGELDLTREGAHSKRRILHARGDATGEEIRESLTRAITKSKNISLKEENFMID
LVKLNRGNHVDGVLVWDNNQKKYIVYLARAVILASGGCGQVYHNTSNPEVTTGDGVAAAYRAGAIIMDMEFIQFHPTVFY
NKHGSSFLISESLRGEGAVLRNSEGKRFMDKYHDLAELAPRDVVARAILQEMERDNKPHVWLDITHKDADFVKNRFPTIF
RTLKEHGIDITRDWVPVVPAAHYMMGGVRTDTYGRTNLIGLYAVGEVACTGVHGANRLASNSLLEGLVFGNRIYRALKME
ADVLPLPEENLKVPQLLPYGKVMVIKQIKEELKKKMSHQVGIIRSEKTLKDMIKWIEETEVKLNKFKYSNVELWELKNML
SVAGIISRSALLRKESRGGHFREDYPEIKPGWGNKHVLVDKLSPEGRIHEVK
>Mature_531_residues
GDIKYKTTDFVIIGTGIAGLYTALKLASLGEVSVLTKEKLEDSNTQYAQGGIAAVIDRGDSWKLHMEDTLKAGAGICDEK
AVEVLVTEGPDRVRELIKMGTRFDHIEGELDLTREGAHSKRRILHARGDATGEEIRESLTRAITKSKNISLKEENFMIDL
VKLNRGNHVDGVLVWDNNQKKYIVYLARAVILASGGCGQVYHNTSNPEVTTGDGVAAAYRAGAIIMDMEFIQFHPTVFYN
KHGSSFLISESLRGEGAVLRNSEGKRFMDKYHDLAELAPRDVVARAILQEMERDNKPHVWLDITHKDADFVKNRFPTIFR
TLKEHGIDITRDWVPVVPAAHYMMGGVRTDTYGRTNLIGLYAVGEVACTGVHGANRLASNSLLEGLVFGNRIYRALKMEA
DVLPLPEENLKVPQLLPYGKVMVIKQIKEELKKKMSHQVGIIRSEKTLKDMIKWIEETEVKLNKFKYSNVELWELKNMLS
VAGIISRSALLRKESRGGHFREDYPEIKPGWGNKHVLVDKLSPEGRIHEVK

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=525, Percent_Identity=32, Blast_Score=219, Evalue=5e-57,
Organism=Escherichia coli, GI1788928, Length=529, Percent_Identity=44.9905482041588, Blast_Score=417, Evalue=1e-118,
Organism=Escherichia coli, GI1786942, Length=547, Percent_Identity=32.3583180987203, Blast_Score=231, Evalue=7e-62,
Organism=Escherichia coli, GI1790597, Length=517, Percent_Identity=31.1411992263056, Blast_Score=218, Evalue=7e-58,
Organism=Caenorhabditis elegans, GI17505833, Length=526, Percent_Identity=32.319391634981, Blast_Score=213, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI17550100, Length=529, Percent_Identity=31.758034026465, Blast_Score=210, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI71986328, Length=260, Percent_Identity=30.3846153846154, Blast_Score=79, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6322701, Length=562, Percent_Identity=33.9857651245552, Blast_Score=251, Evalue=3e-67,
Organism=Saccharomyces cerevisiae, GI6322416, Length=548, Percent_Identity=34.8540145985401, Blast_Score=246, Evalue=5e-66,
Organism=Saccharomyces cerevisiae, GI6320788, Length=469, Percent_Identity=30.2771855010661, Blast_Score=118, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6322511, Length=321, Percent_Identity=29.2834890965732, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24663005, Length=550, Percent_Identity=34.1818181818182, Blast_Score=243, Evalue=2e-64,
Organism=Drosophila melanogaster, GI17137288, Length=518, Percent_Identity=32.2393822393822, Blast_Score=223, Evalue=2e-58,
Organism=Drosophila melanogaster, GI24655642, Length=518, Percent_Identity=32.2393822393822, Blast_Score=223, Evalue=2e-58,
Organism=Drosophila melanogaster, GI24655647, Length=518, Percent_Identity=32.2393822393822, Blast_Score=223, Evalue=2e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 59700; Mature: 59568

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDIKYKTTDFVIIGTGIAGLYTALKLASLGEVSVLTKEKLEDSNTQYAQGGIAAVIDRG
CCCCEEECCCEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCEEEEEECC
DSWKLHMEDTLKAGAGICDEKAVEVLVTEGPDRVRELIKMGTRFDHIEGELDLTREGAHS
CCEEEEHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHCCCCCCCCCCEEECCCCCCH
KRRILHARGDATGEEIRESLTRAITKSKNISLKEENFMIDLVKLNRGNHVDGVLVWDNNQ
HHHEEEECCCCCHHHHHHHHHHHHHHCCCCEEECCCEEEEEEEECCCCCCCEEEEEECCC
KKYIVYLARAVILASGGCGQVYHNTSNPEVTTGDGVAAAYRAGAIIMDMEFIQFHPTVFY
CEEEHHEEHHHHHCCCCCCCCEECCCCCCEECCCCHHHHHHCCEEEEEEHHHEECCEEEE
NKHGSSFLISESLRGEGAVLRNSEGKRFMDKYHDLAELAPRDVVARAILQEMERDNKPHV
CCCCCEEEEECCCCCCCCEEECCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEE
WLDITHKDADFVKNRFPTIFRTLKEHGIDITRDWVPVVPAAHYMMGGVRTDTYGRTNLIG
EEEEECCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHCCCEECCCCCCCEEE
LYAVGEVACTGVHGANRLASNSLLEGLVFGNRIYRALKMEADVLPLPEENLKVPQLLPYG
EEEECCHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
KVMVIKQIKEELKKKMSHQVGIIRSEKTLKDMIKWIEETEVKLNKFKYSNVELWELKNML
HHHHHHHHHHHHHHHHHHHHCEEECHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHH
SVAGIISRSALLRKESRGGHFREDYPEIKPGWGNKHVLVDKLSPEGRIHEVK
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEECCC
>Mature Secondary Structure 
GDIKYKTTDFVIIGTGIAGLYTALKLASLGEVSVLTKEKLEDSNTQYAQGGIAAVIDRG
CCCEEECCCEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCEEEEEECC
DSWKLHMEDTLKAGAGICDEKAVEVLVTEGPDRVRELIKMGTRFDHIEGELDLTREGAHS
CCEEEEHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHCCCCCCCCCCEEECCCCCCH
KRRILHARGDATGEEIRESLTRAITKSKNISLKEENFMIDLVKLNRGNHVDGVLVWDNNQ
HHHEEEECCCCCHHHHHHHHHHHHHHCCCCEEECCCEEEEEEEECCCCCCCEEEEEECCC
KKYIVYLARAVILASGGCGQVYHNTSNPEVTTGDGVAAAYRAGAIIMDMEFIQFHPTVFY
CEEEHHEEHHHHHCCCCCCCCEECCCCCCEECCCCHHHHHHCCEEEEEEHHHEECCEEEE
NKHGSSFLISESLRGEGAVLRNSEGKRFMDKYHDLAELAPRDVVARAILQEMERDNKPHV
CCCCCEEEEECCCCCCCCEEECCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEE
WLDITHKDADFVKNRFPTIFRTLKEHGIDITRDWVPVVPAAHYMMGGVRTDTYGRTNLIG
EEEEECCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHCCCEECCCCCCCEEE
LYAVGEVACTGVHGANRLASNSLLEGLVFGNRIYRALKMEADVLPLPEENLKVPQLLPYG
EEEECCHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
KVMVIKQIKEELKKKMSHQVGIIRSEKTLKDMIKWIEETEVKLNKFKYSNVELWELKNML
HHHHHHHHHHHHHHHHHHHHCEEECHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHH
SVAGIISRSALLRKESRGGHFREDYPEIKPGWGNKHVLVDKLSPEGRIHEVK
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]