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The map label for this gene is rlmL [H]

Identifier: 220919216

GI number: 220919216

Start: 4624243

End: 4625196

Strand: Direct

Name: rlmL [H]

Synonym: A2cp1_4137

Alternate gene names: 220919216

Gene position: 4624243-4625196 (Clockwise)

Preceding gene: 220919215

Following gene: 220919217

Centisome position: 91.95

GC content: 81.03

Gene sequence:

>954_bases
TTGGCCGTGCGCGCCGCCGAGCGGATCTTCTGCGCCTGCGCGCCGGGCCTCGAGGCGGTGCTGGCCGCCGAGCTGCGCGC
GCTGGGCCTTGAGGCGCGCGCCGTGGACGGCGGCGCCGAGGCGGTGGGCGAGGACGCCGCCGCGCTCGCGTGCCTGGGCG
CGCGCACCGCCGACACCGCGCTCCTCCGGCTCTGGGAAGGTTCCGCCGCCGAGCTGCCGGCCGCGAAGCGCGCCGCCGCG
GCCCGGGCCGGCGGGCTCGAGCTCCTGGTGCGCGCCCGGGGTGGGCACGCGACGGTGAGCGTGGACGCGGCCGGCGCGCC
GCTGTTCCGGCGCGGGTGGCGGGCGCGGGTGGGCGCGGCGCCGCTCCGCGAGTCTCTCGCCGCCGGCATCCTGCTGGCGT
GCGGCTGGACCGGCGACCGGCCGTTCCTCGATCCGATGTGCGGCTCGGGCACGCTCGCCATCGAGGCCGCGCTCGTCGCC
GCCGGCCGCGCCCCGGGCCTCGGCCGGACCTTCGCGTTCGAGCGCCTCCCCGGCCACGACGCGGCGCGCACCGCGCGGCT
GCGCGCACACCTCGAGGCGCGGTCCCGGCCGGTGAGCGTGCCGATCCACGCCTCCGACCGGAACGCCGGCGCGCTCCGGC
TCGCGCAGAAGAACGCGGCCGCGGCCGGCGTGGCGGACGCGATCGCGTTCGCGCGAGAGGACGCCGCGCGGGTGGTGCCG
CCGCCCGGCCCGGGGCTGTGCGCGGTGAACCCGCCCTACGGCGTGCGCCTCGACCAGGACGCGGCCGGCGCCTGGCGTGC
GCTGGCCACGCTCATGGGCCGGCTCGCCGGATGGGAGGTGGCGGTGCTCGGGCCCGACCGCGGGCTCGAGCGGCTGCTCG
CGCGCCCGCCCTCCGAGGCGCTGCCGGTCCAGAACGGCGGGATCCGCTGCCGGCTGCTGCGCTGGCGCGGCTGA

Upstream 100 bases:

>100_bases
TCGAGATCCGGGCCGAGGCCGCCGGTCGCCGCGCCTCGGCGCGGGTCGCCTGCCGCGCCGACGCGCTGGTGAAGATCACG
CTCCGGCTCGAGCGCTAGCC

Downstream 100 bases:

>100_bases
TCGTCCCGCGCGGGCAGGCGGCCAGGCGGGCAGGCCGCCGCGCGCACGGGTCCGCATCGTGTGCCCGCATGTGGAGGGAG
CTCGCCGCCCGGCTCACCCG

Product: RNA methylase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MAVRAAERIFCACAPGLEAVLAAELRALGLEARAVDGGAEAVGEDAAALACLGARTADTALLRLWEGSAAELPAAKRAAA
ARAGGLELLVRARGGHATVSVDAAGAPLFRRGWRARVGAAPLRESLAAGILLACGWTGDRPFLDPMCGSGTLAIEAALVA
AGRAPGLGRTFAFERLPGHDAARTARLRAHLEARSRPVSVPIHASDRNAGALRLAQKNAAAAGVADAIAFAREDAARVVP
PPGPGLCAVNPPYGVRLDQDAAGAWRALATLMGRLAGWEVAVLGPDRGLERLLARPPSEALPVQNGGIRCRLLRWRG

Sequences:

>Translated_317_residues
MAVRAAERIFCACAPGLEAVLAAELRALGLEARAVDGGAEAVGEDAAALACLGARTADTALLRLWEGSAAELPAAKRAAA
ARAGGLELLVRARGGHATVSVDAAGAPLFRRGWRARVGAAPLRESLAAGILLACGWTGDRPFLDPMCGSGTLAIEAALVA
AGRAPGLGRTFAFERLPGHDAARTARLRAHLEARSRPVSVPIHASDRNAGALRLAQKNAAAAGVADAIAFAREDAARVVP
PPGPGLCAVNPPYGVRLDQDAAGAWRALATLMGRLAGWEVAVLGPDRGLERLLARPPSEALPVQNGGIRCRLLRWRG
>Mature_316_residues
AVRAAERIFCACAPGLEAVLAAELRALGLEARAVDGGAEAVGEDAAALACLGARTADTALLRLWEGSAAELPAAKRAAAA
RAGGLELLVRARGGHATVSVDAAGAPLFRRGWRARVGAAPLRESLAAGILLACGWTGDRPFLDPMCGSGTLAIEAALVAA
GRAPGLGRTFAFERLPGHDAARTARLRAHLEARSRPVSVPIHASDRNAGALRLAQKNAAAAGVADAIAFAREDAARVVPP
PGPGLCAVNPPYGVRLDQDAAGAWRALATLMGRLAGWEVAVLGPDRGLERLLARPPSEALPVQNGGIRCRLLRWRG

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG0116

COG function: function code L; Predicted N6-adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=227, Percent_Identity=37.4449339207048, Blast_Score=131, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 32562; Mature: 32431

Theoretical pI: Translated: 10.62; Mature: 10.62

Prosite motif: PS00092 N6_MTASE ; PS01261 UPF0020

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVRAAERIFCACAPGLEAVLAAELRALGLEARAVDGGAEAVGEDAAALACLGARTADTA
CCCCCCHHEEEEECCCHHHHHHHHHHHHCCEEEECCCCHHHHCCCHHHHHHHCCCCHHHH
LLRLWEGSAAELPAAKRAAAARAGGLELLVRARGGHATVSVDAAGAPLFRRGWRARVGAA
HHEEECCCCCCCCHHHHHHHHHCCCEEEEEEECCCCEEEEEECCCCHHHHHCCHHHCCCH
PLRESLAAGILLACGWTGDRPFLDPMCGSGTLAIEAALVAAGRAPGLGRTFAFERLPGHD
HHHHHHHCCEEEEECCCCCCCCCCCCCCCCCEEEHHHHHHCCCCCCCCCEEEECCCCCCH
AARTARLRAHLEARSRPVSVPIHASDRNAGALRLAQKNAAAAGVADAIAFAREDAARVVP
HHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEECC
PPGPGLCAVNPPYGVRLDQDAAGAWRALATLMGRLAGWEVAVLGPDRGLERLLARPPSEA
CCCCCEEEECCCCCCEECCCHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHCCCCCCC
LPVQNGGIRCRLLRWRG
CCCCCCCEEEEEEEECC
>Mature Secondary Structure 
AVRAAERIFCACAPGLEAVLAAELRALGLEARAVDGGAEAVGEDAAALACLGARTADTA
CCCCCHHEEEEECCCHHHHHHHHHHHHCCEEEECCCCHHHHCCCHHHHHHHCCCCHHHH
LLRLWEGSAAELPAAKRAAAARAGGLELLVRARGGHATVSVDAAGAPLFRRGWRARVGAA
HHEEECCCCCCCCHHHHHHHHHCCCEEEEEEECCCCEEEEEECCCCHHHHHCCHHHCCCH
PLRESLAAGILLACGWTGDRPFLDPMCGSGTLAIEAALVAAGRAPGLGRTFAFERLPGHD
HHHHHHHCCEEEEECCCCCCCCCCCCCCCCCEEEHHHHHHCCCCCCCCCEEEECCCCCCH
AARTARLRAHLEARSRPVSVPIHASDRNAGALRLAQKNAAAAGVADAIAFAREDAARVVP
HHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEECC
PPGPGLCAVNPPYGVRLDQDAAGAWRALATLMGRLAGWEVAVLGPDRGLERLLARPPSEA
CCCCCEEEECCCCCCEECCCHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHCCCCCCC
LPVQNGGIRCRLLRWRG
CCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA