Click here to switch to the map view.

The map label for this gene is dus1 [H]

Identifier: 220919018

GI number: 220919018

Start: 4375279

End: 4376325

Strand: Direct

Name: dus1 [H]

Synonym: A2cp1_3933

Alternate gene names: 220919018

Gene position: 4375279-4376325 (Clockwise)

Preceding gene: 220919016

Following gene: 220919019

Centisome position: 87.0

GC content: 76.5

Gene sequence:

>1047_bases
ATGCAGATCGGCCCCTACCAGTTCCCCGGCCGCTACTTCCTCGCCCCGCTGGCCGGCGTGTCCGACCGCCCGTTCCGCGC
CATCTGCCGGCGCATGGGCGCGAGCTTCGCGTACACCGAGATGGTCTCGGCGCACGGCCTGATGCACGGCGCGCTGCAGA
CCTCGAGCTACCTCGACCGCGAGCCGGACGAGGACCCGTTCGCGGTACAGATCTTCGCCTCGGAGCCGGAGGTGCTCGCA
CGCGGGGCCGCGGTCGCGGTCGCGGCGGGCGCCGGCATGATCGACGTCAACATGGCCTGCCCGGTGAAGAAGGTGTGCGG
CAGCGGCGCCGGGGCGGCCATGGGGCGCGATCCGCGCAGGGTCGAGGAGGCGGTCCGCGCCATCCGCGCCGCGGTCCCGG
TACCGGTCACGGTCAAGATCCGCGCCGGCTGGGACGAGGGCGAGGTGAACTGCGTCGACGTCGCGCGCGCGGCCGAGGCC
GGCGGCGCCGCGGCGGTGGCGCTGCACGGGCGCACCCGCAGCCAGCTCTACTCCGGCAAGGCGCGCTGGGAGCTGGTCCG
GGCGGTGAAGCAGGCGGTCGCGATCCCGGTGCTCGGCTCGGGGGACGTGTGGACCGCCGCCGACGCCGTGCGCATGCGGG
AGGAGACCGGGTGCGACGCGGTGCTGGTGGCGCGCGGCGCCTGCGGCAACCCGTGGATCTTCCGCGAGCTGCGGGCCATC
GAGGCCGGCCGCCCGCCGCCCCCGCCGCCCGGCCGCGACGAGTGGGTGGGCACGGTGCTCGAGCACGTCCGCCTCCAGGT
GGAGCACCGGGCCCGCATGGCGCCGGGCGAGCCCGCCGAGGCCTCGGAGCGCCACGCCATCCGCGAGCTGCGCAAGCACC
TGCTCTGGTACACGCGCGGCCGGCGCGGCGGCGTGCACTTCCGCCGCGACGCGGCCGCGCTGGTGACCGCGGCCGACGTG
GCGGCGCAGCTGGAGCGCCACTTCCCCGCGGGCGGCGACAGCTTCGAGCTCGATCCCGGCTTCCGCGCCTCGGAGGACGG
CGAGTGA

Upstream 100 bases:

>100_bases
GGTACCGCCCTGGAACCGCCGGCACCCCCGGGCATTCCGGCGTTCCCCACCGAAATCGGTCCGTGCTACACCCCCGACCC
CGCTTGCCGCACGCCTGACC

Downstream 100 bases:

>100_bases
GCGCGCGCGGACCGCACGGCGGCCGGCGGGGATCGGCGCGGTGAAGGCGCTGCTCCTCGAGGGCGCGCCCGACGCGCTCC
GGCGCGCCTGCGAGGCGGGG

Product: TIM-barrel protein, nifR3 family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MQIGPYQFPGRYFLAPLAGVSDRPFRAICRRMGASFAYTEMVSAHGLMHGALQTSSYLDREPDEDPFAVQIFASEPEVLA
RGAAVAVAAGAGMIDVNMACPVKKVCGSGAGAAMGRDPRRVEEAVRAIRAAVPVPVTVKIRAGWDEGEVNCVDVARAAEA
GGAAAVALHGRTRSQLYSGKARWELVRAVKQAVAIPVLGSGDVWTAADAVRMREETGCDAVLVARGACGNPWIFRELRAI
EAGRPPPPPPGRDEWVGTVLEHVRLQVEHRARMAPGEPAEASERHAIRELRKHLLWYTRGRRGGVHFRRDAAALVTAADV
AAQLERHFPAGGDSFELDPGFRASEDGE

Sequences:

>Translated_348_residues
MQIGPYQFPGRYFLAPLAGVSDRPFRAICRRMGASFAYTEMVSAHGLMHGALQTSSYLDREPDEDPFAVQIFASEPEVLA
RGAAVAVAAGAGMIDVNMACPVKKVCGSGAGAAMGRDPRRVEEAVRAIRAAVPVPVTVKIRAGWDEGEVNCVDVARAAEA
GGAAAVALHGRTRSQLYSGKARWELVRAVKQAVAIPVLGSGDVWTAADAVRMREETGCDAVLVARGACGNPWIFRELRAI
EAGRPPPPPPGRDEWVGTVLEHVRLQVEHRARMAPGEPAEASERHAIRELRKHLLWYTRGRRGGVHFRRDAAALVTAADV
AAQLERHFPAGGDSFELDPGFRASEDGE
>Mature_348_residues
MQIGPYQFPGRYFLAPLAGVSDRPFRAICRRMGASFAYTEMVSAHGLMHGALQTSSYLDREPDEDPFAVQIFASEPEVLA
RGAAVAVAAGAGMIDVNMACPVKKVCGSGAGAAMGRDPRRVEEAVRAIRAAVPVPVTVKIRAGWDEGEVNCVDVARAAEA
GGAAAVALHGRTRSQLYSGKARWELVRAVKQAVAIPVLGSGDVWTAADAVRMREETGCDAVLVARGACGNPWIFRELRAI
EAGRPPPPPPGRDEWVGTVLEHVRLQVEHRARMAPGEPAEASERHAIRELRKHLLWYTRGRRGGVHFRRDAAALVTAADV
AAQLERHFPAGGDSFELDPGFRASEDGE

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=232, Percent_Identity=33.6206896551724, Blast_Score=142, Evalue=5e-34,
Organism=Homo sapiens, GI239788462, Length=232, Percent_Identity=33.6206896551724, Blast_Score=141, Evalue=9e-34,
Organism=Homo sapiens, GI40807366, Length=256, Percent_Identity=30.859375, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI31742496, Length=250, Percent_Identity=28.8, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI8923374, Length=257, Percent_Identity=28.7937743190661, Blast_Score=92, Evalue=6e-19,
Organism=Escherichia coli, GI1789660, Length=329, Percent_Identity=36.7781155015198, Blast_Score=202, Evalue=2e-53,
Organism=Escherichia coli, GI1788462, Length=300, Percent_Identity=31.3333333333333, Blast_Score=134, Evalue=9e-33,
Organism=Escherichia coli, GI145693211, Length=169, Percent_Identity=31.9526627218935, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17543114, Length=235, Percent_Identity=30.2127659574468, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI25144369, Length=213, Percent_Identity=31.4553990610329, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17510279, Length=246, Percent_Identity=29.6747967479675, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17507177, Length=230, Percent_Identity=27.8260869565217, Blast_Score=90, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323433, Length=262, Percent_Identity=29.0076335877863, Blast_Score=104, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6323560, Length=230, Percent_Identity=28.2608695652174, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6323437, Length=226, Percent_Identity=24.3362831858407, Blast_Score=83, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24585320, Length=242, Percent_Identity=33.0578512396694, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24580595, Length=227, Percent_Identity=29.9559471365639, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI19920448, Length=227, Percent_Identity=29.9559471365639, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI19921524, Length=226, Percent_Identity=30.5309734513274, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI45549423, Length=316, Percent_Identity=29.1139240506329, Blast_Score=103, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37384; Mature: 37384

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIGPYQFPGRYFLAPLAGVSDRPFRAICRRMGASFAYTEMVSAHGLMHGALQTSSYLDR
CCCCCCCCCCCEEEHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC
EPDEDPFAVQIFASEPEVLARGAAVAVAAGAGMIDVNMACPVKKVCGSGAGAAMGRDPRR
CCCCCCEEEEEECCCCHHHHCCCEEEEECCCCEEEECCCCCHHHHHCCCCCCCCCCCHHH
VEEAVRAIRAAVPVPVTVKIRAGWDEGEVNCVDVARAAEAGGAAAVALHGRTRSQLYSGK
HHHHHHHHHHHCCCCEEEEEEECCCCCCEEHHHHHHHHCCCCEEEEEECCCHHHHHHCCH
ARWELVRAVKQAVAIPVLGSGDVWTAADAVRMREETGCDAVLVARGACGNPWIFRELRAI
HHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHH
EAGRPPPPPPGRDEWVGTVLEHVRLQVEHRARMAPGEPAEASERHAIRELRKHLLWYTRG
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
RRGGVHFRRDAAALVTAADVAAQLERHFPAGGDSFELDPGFRASEDGE
CCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQIGPYQFPGRYFLAPLAGVSDRPFRAICRRMGASFAYTEMVSAHGLMHGALQTSSYLDR
CCCCCCCCCCCEEEHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC
EPDEDPFAVQIFASEPEVLARGAAVAVAAGAGMIDVNMACPVKKVCGSGAGAAMGRDPRR
CCCCCCEEEEEECCCCHHHHCCCEEEEECCCCEEEECCCCCHHHHHCCCCCCCCCCCHHH
VEEAVRAIRAAVPVPVTVKIRAGWDEGEVNCVDVARAAEAGGAAAVALHGRTRSQLYSGK
HHHHHHHHHHHCCCCEEEEEEECCCCCCEEHHHHHHHHCCCCEEEEEECCCHHHHHHCCH
ARWELVRAVKQAVAIPVLGSGDVWTAADAVRMREETGCDAVLVARGACGNPWIFRELRAI
HHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHH
EAGRPPPPPPGRDEWVGTVLEHVRLQVEHRARMAPGEPAEASERHAIRELRKHLLWYTRG
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
RRGGVHFRRDAAALVTAADVAAQLERHFPAGGDSFELDPGFRASEDGE
CCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]