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The map label for this gene is murD [H]

Identifier: 220918994

GI number: 220918994

Start: 4348570

End: 4349958

Strand: Direct

Name: murD [H]

Synonym: A2cp1_3909

Alternate gene names: 220918994

Gene position: 4348570-4349958 (Clockwise)

Preceding gene: 220918993

Following gene: 220918995

Centisome position: 86.46

GC content: 77.32

Gene sequence:

>1389_bases
GTGGCCGCACCGGACCTGAAGGGCACGAGGGTGACGGTGGTGGGGCTCGCGAAGAGCGGCGTCGCCGCCGCGCGGCTCTG
CGCCCGCGAGGGCGCGCGCGTCACCGTCACCGACCGCCGCGGCGAGGCCGAGCTGGGCGGCGCGCTCGCCGCGCTCCCGG
CCTCGGTGACCCGCCACCTGGGCGGCCACGACCCCGCCGACTTCACCGGCGCCGAGCTGGTCGTGGCGTCGCCGGGCGTG
CCGCTCGCGAACCCGGAGATCCAGGAGGCGCGGCGGCGCGGCGTGGCGGTGTGGGGCGAGGTCGAGCTGGCCGCGCGCTT
CCTGGGCGGCCTGCCGATCGTCGGGATCACCGGGACGAACGGCAAGTCGACCACCACCGCGCTCACCGGCGCGCTGCTCG
CCCGCGACCGGCGGACGTTCGTGGGCGGCAACCTGGGCACCCCGCTCTCCGAGCTGGTCCTGTCCGGCGAGCCCGCCGAC
GCCGCGGTGGTCGAGCTGTCCTCCTTCCAGCTGGAGGGGATCGAGCGCTTCCGCGCCCGGGTGGCGGCGGTCCTGAACGT
CACCCCCGACCACCTCGACCGCTACCCCGACGTGGACGCGTACGCGGCCGCCAAGGCGCGGCTGTTCGCCACGCAGCAGC
CGGACGACGTGGCCGTCGCGAACGCGCGCGACCCGCGGGCGCTCGCCATGGCCGGCGCGTCCCGCGCCGACCTGCACACC
TTCGGCTTCGGCGCGCCCACCCCCGCCTCCGCCCGCGACGAGGGCGGCGAGCCCGGGCCCGAGGGCACCGCCATCTGGTT
CACGCCGCGCGGGCGCGCGCCCGAGCGCTACCAGCTCCGCAACCGCGCGCTGCGCGGCCGGCACAACCGCGAGAACGCCA
TGGCCGCCGTGCTCTGCGCGCGCCTCATGGGCGTGCCCGGCGAGGCGGTCCAGGCCGGGCTCGACGCGTTCCCCGGCCTG
CACCACCGGCTCGAGCTGGTCGCGGAGGGGCGCGGGGTGGAGTGGGTGAACGACTCCAAGGCCACCAACGTGGACTCCAC
CTTCGTCGGCCTGGCCGCGTTCCCGTCCGGCGCCCCGCGCGTGGTCCTCATCATGGGCGGCCGCGGCAAGAAGGCGCCCT
ACGCGCCGCTGCGCCCGCTGTTCGGCGGGCGAGTCAAGGCGCTGCTCACCATCGGGGAGGACGCGCCCGCCATCGAGCGG
GAGCTGGGCGACCTCGCGCCCACCGAGCCGTGCGGCGACCTGGCCGACGCGGTCCGCCGGGCGGCGGTCCTCTCGGGGCC
GGGCGACGTGGTGCTGCTCTCCCCCGCCTGCGCCAGCTACGATCAGTTCGCGAACTACGAGGAGCGCGGCGAGGCCTTCC
GCCGGCTCGCGACGGAGCAGGCGCGATGA

Upstream 100 bases:

>100_bases
AGCTGAAGGGGTGGGCGGAGCCGAAGATCATCGTCCGCTTCTGGATCATCTCGGTGATGCTGGCGCTGGTTGCGCTGCTC
TCCATCAAGCTGAGGTAGCC

Downstream 100 bases:

>100_bases
GGCTCTTCCGCCACGCCCGGGAGGAACGCGCGCCCGAGGCGCCGCGGGCGGGCGCGCCCGGCTTCGACCCGCTCCTGATC
TCGGCGGTGCTGCTGCTCGT

Product: UDP-N-acetylmuramoylalanine/D-glutamate ligase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MAAPDLKGTRVTVVGLAKSGVAAARLCAREGARVTVTDRRGEAELGGALAALPASVTRHLGGHDPADFTGAELVVASPGV
PLANPEIQEARRRGVAVWGEVELAARFLGGLPIVGITGTNGKSTTTALTGALLARDRRTFVGGNLGTPLSELVLSGEPAD
AAVVELSSFQLEGIERFRARVAAVLNVTPDHLDRYPDVDAYAAAKARLFATQQPDDVAVANARDPRALAMAGASRADLHT
FGFGAPTPASARDEGGEPGPEGTAIWFTPRGRAPERYQLRNRALRGRHNRENAMAAVLCARLMGVPGEAVQAGLDAFPGL
HHRLELVAEGRGVEWVNDSKATNVDSTFVGLAAFPSGAPRVVLIMGGRGKKAPYAPLRPLFGGRVKALLTIGEDAPAIER
ELGDLAPTEPCGDLADAVRRAAVLSGPGDVVLLSPACASYDQFANYEERGEAFRRLATEQAR

Sequences:

>Translated_462_residues
MAAPDLKGTRVTVVGLAKSGVAAARLCAREGARVTVTDRRGEAELGGALAALPASVTRHLGGHDPADFTGAELVVASPGV
PLANPEIQEARRRGVAVWGEVELAARFLGGLPIVGITGTNGKSTTTALTGALLARDRRTFVGGNLGTPLSELVLSGEPAD
AAVVELSSFQLEGIERFRARVAAVLNVTPDHLDRYPDVDAYAAAKARLFATQQPDDVAVANARDPRALAMAGASRADLHT
FGFGAPTPASARDEGGEPGPEGTAIWFTPRGRAPERYQLRNRALRGRHNRENAMAAVLCARLMGVPGEAVQAGLDAFPGL
HHRLELVAEGRGVEWVNDSKATNVDSTFVGLAAFPSGAPRVVLIMGGRGKKAPYAPLRPLFGGRVKALLTIGEDAPAIER
ELGDLAPTEPCGDLADAVRRAAVLSGPGDVVLLSPACASYDQFANYEERGEAFRRLATEQAR
>Mature_461_residues
AAPDLKGTRVTVVGLAKSGVAAARLCAREGARVTVTDRRGEAELGGALAALPASVTRHLGGHDPADFTGAELVVASPGVP
LANPEIQEARRRGVAVWGEVELAARFLGGLPIVGITGTNGKSTTTALTGALLARDRRTFVGGNLGTPLSELVLSGEPADA
AVVELSSFQLEGIERFRARVAAVLNVTPDHLDRYPDVDAYAAAKARLFATQQPDDVAVANARDPRALAMAGASRADLHTF
GFGAPTPASARDEGGEPGPEGTAIWFTPRGRAPERYQLRNRALRGRHNRENAMAAVLCARLMGVPGEAVQAGLDAFPGLH
HRLELVAEGRGVEWVNDSKATNVDSTFVGLAAFPSGAPRVVLIMGGRGKKAPYAPLRPLFGGRVKALLTIGEDAPAIERE
LGDLAPTEPCGDLADAVRRAAVLSGPGDVVLLSPACASYDQFANYEERGEAFRRLATEQAR

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=460, Percent_Identity=37.6086956521739, Blast_Score=233, Evalue=2e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 48294; Mature: 48163

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: PS00012 PHOSPHOPANTETHEINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAPDLKGTRVTVVGLAKSGVAAARLCAREGARVTVTDRRGEAELGGALAALPASVTRHL
CCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEEECCCCCHHHCCHHHHHHHHHHHHC
GGHDPADFTGAELVVASPGVPLANPEIQEARRRGVAVWGEVELAARFLGGLPIVGITGTN
CCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHCCCEEEEHHHHHHHHHCCCCEEEEECCC
GKSTTTALTGALLARDRRTFVGGNLGTPLSELVLSGEPADAAVVELSSFQLEGIERFRAR
CCCHHHHHHHHHHHHCCHHEECCCCCCCHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHH
VAAVLNVTPDHLDRYPDVDAYAAAKARLFATQQPDDVAVANARDPRALAMAGASRADLHT
HHHHHCCCHHHHHCCCCCCHHHHHHHHEEECCCCCCEEEECCCCCCEEEECCCCCCCEEE
FGFGAPTPASARDEGGEPGPEGTAIWFTPRGRAPERYQLRNRALRGRHNRENAMAAVLCA
CCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHH
RLMGVPGEAVQAGLDAFPGLHHRLELVAEGRGVEWVNDSKATNVDSTFVGLAAFPSGAPR
HHHCCCHHHHHHHHHHCCCHHHHHHHEECCCCCEEECCCCCCCCCHHEEEEEECCCCCCE
VVLIMGGRGKKAPYAPLRPLFGGRVKALLTIGEDAPAIERELGDLAPTEPCGDLADAVRR
EEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHCCCCCCCCHHHHHHHHH
AAVLSGPGDVVLLSPACASYDQFANYEERGEAFRRLATEQAR
HHHCCCCCCEEEECCCHHCHHHHCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AAPDLKGTRVTVVGLAKSGVAAARLCAREGARVTVTDRRGEAELGGALAALPASVTRHL
CCCCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEEECCCCCHHHCCHHHHHHHHHHHHC
GGHDPADFTGAELVVASPGVPLANPEIQEARRRGVAVWGEVELAARFLGGLPIVGITGTN
CCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHCCCEEEEHHHHHHHHHCCCCEEEEECCC
GKSTTTALTGALLARDRRTFVGGNLGTPLSELVLSGEPADAAVVELSSFQLEGIERFRAR
CCCHHHHHHHHHHHHCCHHEECCCCCCCHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHH
VAAVLNVTPDHLDRYPDVDAYAAAKARLFATQQPDDVAVANARDPRALAMAGASRADLHT
HHHHHCCCHHHHHCCCCCCHHHHHHHHEEECCCCCCEEEECCCCCCEEEECCCCCCCEEE
FGFGAPTPASARDEGGEPGPEGTAIWFTPRGRAPERYQLRNRALRGRHNRENAMAAVLCA
CCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHH
RLMGVPGEAVQAGLDAFPGLHHRLELVAEGRGVEWVNDSKATNVDSTFVGLAAFPSGAPR
HHHCCCHHHHHHHHHHCCCHHHHHHHEECCCCCEEECCCCCCCCCHHEEEEEECCCCCCE
VVLIMGGRGKKAPYAPLRPLFGGRVKALLTIGEDAPAIERELGDLAPTEPCGDLADAVRR
EEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHCCCCCCCCHHHHHHHHH
AAVLSGPGDVVLLSPACASYDQFANYEERGEAFRRLATEQAR
HHHCCCCCCEEEECCCHHCHHHHCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA