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The map label for this gene is rlmI [H]

Identifier: 220918947

GI number: 220918947

Start: 4294545

End: 4295714

Strand: Direct

Name: rlmI [H]

Synonym: A2cp1_3862

Alternate gene names: 220918947

Gene position: 4294545-4295714 (Clockwise)

Preceding gene: 220918946

Following gene: 220918948

Centisome position: 85.39

GC content: 74.36

Gene sequence:

>1170_bases
ATGGACGAGCGGAAGGTGACGGTCACGCGCCGCGGCGCCGAGCGGATCGCCCGGGGCCACCTCTGGATCTACCGGACGGA
CGTGGAGAAGGCCCCGGCGGGCGCCGAGGCGGGCGACGTGGTGGCGCTGGTGGACGGGCGCGGGCGGTTCCTGGGGAAGG
CGTTCTGGTCGGCGCGCTCCAAGATCGCGCTGCGGCTCGTCACCCGCGACGAGGTCCCGGTGGACGAGGCCTTCCTCGCC
GCGCGCCTGTCGGACGCCATCGCGCTGCGGCGGCGCGTCTTCGGCGAGGAGCGCTTCGTGCGGCTGGTGCACGGCGAGGC
CGACCTGTTCCCGGGCCTGGTGGCGGACCGCTACGGCGACGTGGGGGTGGTGCAGACGCTCGTGCCCGCCACCGACCGGC
GCAAGGGGCTCATCGCCGAGCTGCTCGCGGGCGCGCTCGACCTGCGCACGGTGGTGGAGCGGAACGACGTGCGCGTGCGC
GAGCTCGAGGGGCTGGAGCAGGTGAAGGGCGTGGTGCGCGGCCCTGCGCCCGGGCCGCTCGAGTACCGCGAGGGCGAGGT
CCGGATGCGGCTGGACGTGCTCGCCGGCCAGAAGACCGGCGCGTTCCTCGACCAGCGCGAGAACCACCTGCGCGCCGGGG
AGTACGCCACCGGCCGCTGCCTGGACTGCTTCAGCTACGCGGGCGGCTTCGCGCTCCACCTCGCGCGCCGCGCCGAGCGC
GTCACCGCGGTGGAGATGCAGCCGGTCGCGGCCGGGCTCCTGCGCGAGAACGCGGCGCTGAACCGCGCCGAGAACCTGGA
GGTCGTCGAGGAGAACGCCTTCGACTACCTGCGCGACCGCTCCGAGGAGGAGCCCGCCTTCGACCTGGTGGTGCTCGACC
CGCCCGCGTTCGCGAAGAACAAGGAGTCGCTGCCGGCGGCGCGGCGCGGGTACAAGGAGGTGAACCTCCGCGCGCTCCAG
GTGCTGGTGCCGGGCGGCATCCTGGTGACCGCCTCGTGCAGCTATCACCTGTCCCCCGAGATGCTGGAGGAGCTGGTGCT
CGACGCCGCCAACGACGCCGGGCGCCGCGTGCAGGTGCTCGAGCGGCGCGGCGCCGGCCGAGACCACCCCGAGCTCGCCG
GCGTGCCGGAGACCCGGTATCTGAAGGCCCTGTTCCTGCGGGTGCTGTGA

Upstream 100 bases:

>100_bases
GCGGACGGCCTGCCGGCGTTCCGGGAATCGGTCGAGCTGTAGCGGGCCTGCGCCGGTCTCCGCTGGCGCCGGAGCGCGTC
CTCGGGTACGGAGTGCCGCC

Downstream 100 bases:

>100_bases
GGCGCTTCCGCCGGCCCCCCGTCCGCCCGGCCGCCGGGGGCGCGCACGCAGGGACCGGGTGCGCTTTCGCGCGCACCCTG
GACGCGCTAGCATCGTAGGC

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MDERKVTVTRRGAERIARGHLWIYRTDVEKAPAGAEAGDVVALVDGRGRFLGKAFWSARSKIALRLVTRDEVPVDEAFLA
ARLSDAIALRRRVFGEERFVRLVHGEADLFPGLVADRYGDVGVVQTLVPATDRRKGLIAELLAGALDLRTVVERNDVRVR
ELEGLEQVKGVVRGPAPGPLEYREGEVRMRLDVLAGQKTGAFLDQRENHLRAGEYATGRCLDCFSYAGGFALHLARRAER
VTAVEMQPVAAGLLRENAALNRAENLEVVEENAFDYLRDRSEEEPAFDLVVLDPPAFAKNKESLPAARRGYKEVNLRALQ
VLVPGGILVTASCSYHLSPEMLEELVLDAANDAGRRVQVLERRGAGRDHPELAGVPETRYLKALFLRVL

Sequences:

>Translated_389_residues
MDERKVTVTRRGAERIARGHLWIYRTDVEKAPAGAEAGDVVALVDGRGRFLGKAFWSARSKIALRLVTRDEVPVDEAFLA
ARLSDAIALRRRVFGEERFVRLVHGEADLFPGLVADRYGDVGVVQTLVPATDRRKGLIAELLAGALDLRTVVERNDVRVR
ELEGLEQVKGVVRGPAPGPLEYREGEVRMRLDVLAGQKTGAFLDQRENHLRAGEYATGRCLDCFSYAGGFALHLARRAER
VTAVEMQPVAAGLLRENAALNRAENLEVVEENAFDYLRDRSEEEPAFDLVVLDPPAFAKNKESLPAARRGYKEVNLRALQ
VLVPGGILVTASCSYHLSPEMLEELVLDAANDAGRRVQVLERRGAGRDHPELAGVPETRYLKALFLRVL
>Mature_389_residues
MDERKVTVTRRGAERIARGHLWIYRTDVEKAPAGAEAGDVVALVDGRGRFLGKAFWSARSKIALRLVTRDEVPVDEAFLA
ARLSDAIALRRRVFGEERFVRLVHGEADLFPGLVADRYGDVGVVQTLVPATDRRKGLIAELLAGALDLRTVVERNDVRVR
ELEGLEQVKGVVRGPAPGPLEYREGEVRMRLDVLAGQKTGAFLDQRENHLRAGEYATGRCLDCFSYAGGFALHLARRAER
VTAVEMQPVAAGLLRENAALNRAENLEVVEENAFDYLRDRSEEEPAFDLVVLDPPAFAKNKESLPAARRGYKEVNLRALQ
VLVPGGILVTASCSYHLSPEMLEELVLDAANDAGRRVQVLERRGAGRDHPELAGVPETRYLKALFLRVL

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=393, Percent_Identity=35.1145038167939, Blast_Score=225, Evalue=4e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 43060; Mature: 43060

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDERKVTVTRRGAERIARGHLWIYRTDVEKAPAGAEAGDVVALVDGRGRFLGKAFWSARS
CCCCEEEEEHHHHHHHHHCCEEEEEECCCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCC
KIALRLVTRDEVPVDEAFLAARLSDAIALRRRVFGEERFVRLVHGEADLFPGLVADRYGD
EEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCHHHEEEEECCCCHHCCCHHHHCCCC
VGVVQTLVPATDRRKGLIAELLAGALDLRTVVERNDVRVRELEGLEQVKGVVRGPAPGPL
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCC
EYREGEVRMRLDVLAGQKTGAFLDQRENHLRAGEYATGRCLDCFSYAGGFALHLARRAER
CCCCCCEEEEEEEECCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHH
VTAVEMQPVAAGLLRENAALNRAENLEVVEENAFDYLRDRSEEEPAFDLVVLDPPAFAKN
HEEEEHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCC
KESLPAARRGYKEVNLRALQVLVPGGILVTASCSYHLSPEMLEELVLDAANDAGRRVQVL
CCCCCHHHCCCHHHCCEEEEEEECCCEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHH
ERRGAGRDHPELAGVPETRYLKALFLRVL
HHCCCCCCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MDERKVTVTRRGAERIARGHLWIYRTDVEKAPAGAEAGDVVALVDGRGRFLGKAFWSARS
CCCCEEEEEHHHHHHHHHCCEEEEEECCCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCC
KIALRLVTRDEVPVDEAFLAARLSDAIALRRRVFGEERFVRLVHGEADLFPGLVADRYGD
EEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCHHHEEEEECCCCHHCCCHHHHCCCC
VGVVQTLVPATDRRKGLIAELLAGALDLRTVVERNDVRVRELEGLEQVKGVVRGPAPGPL
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCC
EYREGEVRMRLDVLAGQKTGAFLDQRENHLRAGEYATGRCLDCFSYAGGFALHLARRAER
CCCCCCEEEEEEEECCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHH
VTAVEMQPVAAGLLRENAALNRAENLEVVEENAFDYLRDRSEEEPAFDLVVLDPPAFAKN
HEEEEHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCC
KESLPAARRGYKEVNLRALQVLVPGGILVTASCSYHLSPEMLEELVLDAANDAGRRVQVL
CCCCCHHHCCCHHHCCEEEEEEECCCEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHH
ERRGAGRDHPELAGVPETRYLKALFLRVL
HHCCCCCCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA