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The map label for this gene is shiA [H]

Identifier: 220918925

GI number: 220918925

Start: 4265270

End: 4266961

Strand: Direct

Name: shiA [H]

Synonym: A2cp1_3839

Alternate gene names: 220918925

Gene position: 4265270-4266961 (Clockwise)

Preceding gene: 220918920

Following gene: 220918928

Centisome position: 84.81

GC content: 65.78

Gene sequence:

>1692_bases
ATGTCCGTGTCGTCGGCATCCCCTGTCCGACCCGTCCCCCCCGACTCCGGGTCACGTGCTGGAATCACCCGCGAAGAGAA
GATGGTCATCTTCGCGTCGTCCCTCGGAACCGTGTTCGAGTGGTACGACTTCTACCTGTACGGCTCGCTCGCGGCGATCA
TCAGCAAGCAGTTCTTCGCCGGCGTCGACGAGCGCACCGGCTTCATCTTCGCGCTGCTCGCCTTCGCCGCCGGCTTCGCG
GTGCGCCCGTTCGGCGCGGTGGTGTTCGGCCGGCTCGGCGACATGATCGGCCGGAAGCACACCTTCCTCCTCACCATCCT
CATCATGGGCGGCTCGACGGCGATCGTCGGCATGCTCCCCAGCTACGCCACGATCGGCGTGCTGGCGCCGATCATCCTCA
TCTCCATGCGCATGCTGCAGGGCCTCGCGCTGGGTGGTGAGTACGGCGGCGCGGCCACGTACGTGGCCGAGCACGCGCCC
CCGGGCCGGCGCGGCAACTTCACGAGCTGGATCCAGACCACCGCCACCCTCGGCCTGTTCCTCTCGCTCCTCATCATCCT
CGGCACCCGCATCGGCCTGGGGAACGAGGCGTTCGAGAAGTGGGGCTGGCGCGTCCCGTTCCTGCTCTCGCTGATCCTGC
TCGGCATCTCCGTCTACATCCGCCTCCAGCTGAACGAGTCCCCGGTGTTCAAGCGGATGAAGGAGGAGGGCAAGGCCTCG
AAGGCCCCGCTCACCGAGTCCTTCCTGCGCTGGAACAACCTGAAGATCGTCCTGATGGTGCTGTTCGGCGGCGTCGCCGG
CCAGGCGGTGGTCTGGTACACGGGCCAGTTCTACGCGCTGTTCTTCCTCCAGCAGACGCTGAAGGTGGACCCGACCGCGT
CGAACTACATGATCGCGGCCTCGCTGATCATCGGCACGCCGTTCTTCATCGTGTTCGGCACGCTGTCCGACAAGATCGGC
CGCAAGCGGATCATCATGGCGGGCTGCGTGCTCGCCGCGCTGACCTACTTCCCGATCTTCAAGGGCATCACGAAGTACGC
GAACCCGGCGCTGGAGCACGCGCAGGCGACGAACCCCGTGACGGTGGTCGCCGATCCGAAGGACTGCAGCTTCCAGTTCG
ACCCGATCGGCAAGGCCAAGTTCACGAAGTCCTGCGACGTCGCCAAGGCCTCGCTCGCGAAGAAGGGCGTGCCGTACCGC
AACGAGGAGGCGCCCGCGGGCACCGTCGCCACCATCAAGATCGGCGACGTGGCGGTCTCGAGCTTCGCCGGCAACACGCT
CTCGCCGGCAGACTTCAAGACGCAGAACGCCGCGTTCGAGAAGACCCTCGGCGACGCCATCAAGACCGCCGGCTACCCCG
CCAAGGCGGACATGAACCAGGTGAACTACGTGATGGTCGTGCTGCTCCTGACCATCCTCGTCATCTACGTGACCATGGTG
TACGCGCCGATCGCGGCGTGGCTGGTCGAGCTCTTCCCGGCTCGCATCCGCTACACCTCGATGTCGCTGCCGTACCACAT
CGGCAACGGCTGGTTCGGCGGCTTCCTGCCGACCATCTCGTTCGCGATGGTCGCCGCCAGCGGCGACATCTACTACGGCC
TCTGGTACCCCATCGTCATCGCGCTCATCACCGCGGTCCTCGGCACCCTGTTCATGCCCGAGACCAAGGATCGCGACATC
AACCACGAGTAG

Upstream 100 bases:

>100_bases
CGGTATTTGCGCGCAGACCGTTGTAAACGGCGAATCCGGTGTGCTACCTCGTACCTCAGGCCACGCGGCTGGCGTACCAA
CCTCACCGTGGAGGAAGAAA

Downstream 100 bases:

>100_bases
CATGAGCGGCGCGCAGTTCGGGATGATGGCCGGTGGCCTGGCCGTGCTGGGCCCCGTCATCTGGGGAGTGGCGGAGGTCA
CCAGCAGGTAGTCGCCGCTC

Product: General substrate transporter

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 563; Mature: 562

Protein sequence:

>563_residues
MSVSSASPVRPVPPDSGSRAGITREEKMVIFASSLGTVFEWYDFYLYGSLAAIISKQFFAGVDERTGFIFALLAFAAGFA
VRPFGAVVFGRLGDMIGRKHTFLLTILIMGGSTAIVGMLPSYATIGVLAPIILISMRMLQGLALGGEYGGAATYVAEHAP
PGRRGNFTSWIQTTATLGLFLSLLIILGTRIGLGNEAFEKWGWRVPFLLSLILLGISVYIRLQLNESPVFKRMKEEGKAS
KAPLTESFLRWNNLKIVLMVLFGGVAGQAVVWYTGQFYALFFLQQTLKVDPTASNYMIAASLIIGTPFFIVFGTLSDKIG
RKRIIMAGCVLAALTYFPIFKGITKYANPALEHAQATNPVTVVADPKDCSFQFDPIGKAKFTKSCDVAKASLAKKGVPYR
NEEAPAGTVATIKIGDVAVSSFAGNTLSPADFKTQNAAFEKTLGDAIKTAGYPAKADMNQVNYVMVVLLLTILVIYVTMV
YAPIAAWLVELFPARIRYTSMSLPYHIGNGWFGGFLPTISFAMVAASGDIYYGLWYPIVIALITAVLGTLFMPETKDRDI
NHE

Sequences:

>Translated_563_residues
MSVSSASPVRPVPPDSGSRAGITREEKMVIFASSLGTVFEWYDFYLYGSLAAIISKQFFAGVDERTGFIFALLAFAAGFA
VRPFGAVVFGRLGDMIGRKHTFLLTILIMGGSTAIVGMLPSYATIGVLAPIILISMRMLQGLALGGEYGGAATYVAEHAP
PGRRGNFTSWIQTTATLGLFLSLLIILGTRIGLGNEAFEKWGWRVPFLLSLILLGISVYIRLQLNESPVFKRMKEEGKAS
KAPLTESFLRWNNLKIVLMVLFGGVAGQAVVWYTGQFYALFFLQQTLKVDPTASNYMIAASLIIGTPFFIVFGTLSDKIG
RKRIIMAGCVLAALTYFPIFKGITKYANPALEHAQATNPVTVVADPKDCSFQFDPIGKAKFTKSCDVAKASLAKKGVPYR
NEEAPAGTVATIKIGDVAVSSFAGNTLSPADFKTQNAAFEKTLGDAIKTAGYPAKADMNQVNYVMVVLLLTILVIYVTMV
YAPIAAWLVELFPARIRYTSMSLPYHIGNGWFGGFLPTISFAMVAASGDIYYGLWYPIVIALITAVLGTLFMPETKDRDI
NHE
>Mature_562_residues
SVSSASPVRPVPPDSGSRAGITREEKMVIFASSLGTVFEWYDFYLYGSLAAIISKQFFAGVDERTGFIFALLAFAAGFAV
RPFGAVVFGRLGDMIGRKHTFLLTILIMGGSTAIVGMLPSYATIGVLAPIILISMRMLQGLALGGEYGGAATYVAEHAPP
GRRGNFTSWIQTTATLGLFLSLLIILGTRIGLGNEAFEKWGWRVPFLLSLILLGISVYIRLQLNESPVFKRMKEEGKASK
APLTESFLRWNNLKIVLMVLFGGVAGQAVVWYTGQFYALFFLQQTLKVDPTASNYMIAASLIIGTPFFIVFGTLSDKIGR
KRIIMAGCVLAALTYFPIFKGITKYANPALEHAQATNPVTVVADPKDCSFQFDPIGKAKFTKSCDVAKASLAKKGVPYRN
EEAPAGTVATIKIGDVAVSSFAGNTLSPADFKTQNAAFEKTLGDAIKTAGYPAKADMNQVNYVMVVLLLTILVIYVTMVY
APIAAWLVELFPARIRYTSMSLPYHIGNGWFGGFLPTISFAMVAASGDIYYGLWYPIVIALITAVLGTLFMPETKDRDIN
HE

Specific function: Unknown

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=338, Percent_Identity=33.7278106508876, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1790550, Length=329, Percent_Identity=34.0425531914894, Blast_Score=178, Evalue=9e-46,
Organism=Escherichia coli, GI1788942, Length=338, Percent_Identity=33.1360946745562, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1789941, Length=222, Percent_Identity=42.7927927927928, Blast_Score=149, Evalue=6e-37,
Organism=Escherichia coli, GI87082231, Length=229, Percent_Identity=22.707423580786, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI87082404, Length=316, Percent_Identity=23.4177215189873, Blast_Score=66, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 61083; Mature: 60952

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVSSASPVRPVPPDSGSRAGITREEKMVIFASSLGTVFEWYDFYLYGSLAAIISKQFFA
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHC
GVDERTGFIFALLAFAAGFAVRPFGAVVFGRLGDMIGRKHTFLLTILIMGGSTAIVGMLP
CCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHEEEEEHHCCCEEEEHHCC
SYATIGVLAPIILISMRMLQGLALGGEYGGAATYVAEHAPPGRRGNFTSWIQTTATLGLF
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
LSLLIILGTRIGLGNEAFEKWGWRVPFLLSLILLGISVYIRLQLNESPVFKRMKEEGKAS
HHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHEEEEEEEECCCHHHHHHHHHCCCC
KAPLTESFLRWNNLKIVLMVLFGGVAGQAVVWYTGQFYALFFLQQTLKVDPTASNYMIAA
CCCHHHHHHHHCCHHEEEHHHHCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCHHEEH
SLIIGTPFFIVFGTLSDKIGRKRIIMAGCVLAALTYFPIFKGITKYANPALEHAQATNPV
HHHHCCCHHEEEECHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCE
TVVADPKDCSFQFDPIGKAKFTKSCDVAKASLAKKGVPYRNEEAPAGTVATIKIGDVAVS
EEEECCCCCCEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCHHHH
SFAGNTLSPADFKTQNAAFEKTLGDAIKTAGYPAKADMNQVNYVMVVLLLTILVIYVTMV
HHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
YAPIAAWLVELFPARIRYTSMSLPYHIGNGWFGGFLPTISFAMVAASGDIYYGLWYPIVI
HHHHHHHHHHHHHHHHEEEEECCCEEECCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHH
ALITAVLGTLFMPETKDRDINHE
HHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SVSSASPVRPVPPDSGSRAGITREEKMVIFASSLGTVFEWYDFYLYGSLAAIISKQFFA
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHC
GVDERTGFIFALLAFAAGFAVRPFGAVVFGRLGDMIGRKHTFLLTILIMGGSTAIVGMLP
CCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHEEEEEHHCCCEEEEHHCC
SYATIGVLAPIILISMRMLQGLALGGEYGGAATYVAEHAPPGRRGNFTSWIQTTATLGLF
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
LSLLIILGTRIGLGNEAFEKWGWRVPFLLSLILLGISVYIRLQLNESPVFKRMKEEGKAS
HHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHEEEEEEEECCCHHHHHHHHHCCCC
KAPLTESFLRWNNLKIVLMVLFGGVAGQAVVWYTGQFYALFFLQQTLKVDPTASNYMIAA
CCCHHHHHHHHCCHHEEEHHHHCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCHHEEH
SLIIGTPFFIVFGTLSDKIGRKRIIMAGCVLAALTYFPIFKGITKYANPALEHAQATNPV
HHHHCCCHHEEEECHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCE
TVVADPKDCSFQFDPIGKAKFTKSCDVAKASLAKKGVPYRNEEAPAGTVATIKIGDVAVS
EEEECCCCCCEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCHHHH
SFAGNTLSPADFKTQNAAFEKTLGDAIKTAGYPAKADMNQVNYVMVVLLLTILVIYVTMV
HHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
YAPIAAWLVELFPARIRYTSMSLPYHIGNGWFGGFLPTISFAMVAASGDIYYGLWYPIVI
HHHHHHHHHHHHHHHHEEEEECCCEEECCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHH
ALITAVLGTLFMPETKDRDINHE
HHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]