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The map label for this gene is pckG

Identifier: 220918903

GI number: 220918903

Start: 4241767

End: 4243557

Strand: Direct

Name: pckG

Synonym: A2cp1_3817

Alternate gene names: 220918903

Gene position: 4241767-4243557 (Clockwise)

Preceding gene: 220918901

Following gene: 220918904

Centisome position: 84.34

GC content: 68.51

Gene sequence:

>1791_bases
ATGTCCACCTCTCCTGCCGCTCGTCCCACGTCGAACGCGCACCTCCTCGGCTGGGTCGACGAGATGGCGAAGCTCTGCAA
GCCCGATCGCGTCTACTGGTGCGACGGCTCCGAGGCCGAGAAGAAGCGGCTGACCGACGACGCCGTCGCCGCCAAGGTCC
TCATCCCGCTCGATCAGCAGAAGTGGCCCGGCTGCCACTACCACCACTCGAACTCGAGCGACGTCGCCCGCGTCGAGCAC
CTCACCTTCATCTGCACGCCGACGAAGGAGCAGGCCGGCCCGACCAACAACTGGATGGAGCCGAAGGAGGCCTACCGGAA
GCTCGGCGCCATCTTCGACGGGTCGATGAAGGGCCGCACCATGTACGTGGTGCCGTACGTGATGGGCCCGAGCACCTCAC
CCTTCGCGAAGGTCGGCATCGAGATCACCGACTCCGTGTACGTGGCCCTGAACATGGGGATCATGGCGCGCATGGGGAAG
GTGGCGCTCGACCGCCTCGGCGACTCGGACGAGTTCAACCGCGGCCTGCACTCGGTCGCCGACTGCAACCCCGAGCGCCG
CTTCATCTGCCACTTCCCGCAGGACAACACCATCTGGTCGGTGGGCTCCGGCTACGGCGGCAACGCGCTGCTCGGCAAGA
AGTGCCTCGCGCTCCGCATCGCCAGCTATCTCGCGAAGAACGAGGGCTGGCTCGCCGAGCACATGCTGATCCTCGAGGCG
GAGAGCCCGACCGGCGAGAAGCAGTACGTCGCGGCCGCGTTCCCGTCGGCCTGCGGCAAGACGAACTTCGCCATGATGAT
CCCGCCCGCCGCGTTCCCGGGCTGGAAGATCCGGACGGTCGGCGACGACATCTCCTGGATGCGGGTGGGCGAGGACGGCC
GCCTCTGGGCGGTGAACCCGGAGAACGGCTACTTCGGCGTGGCCCCGGGCACGAACCGGAAGACGAACCCCAACGCGATG
GACTCGGTCCGCAAGGACACCATCTTCACCAACGTCGCCCGCACGCCGGACGGCGACATCTGGTGGGAGGGGATGGACCA
CGAGGCGCCGGCCGAGCTGATCGACTGGAAGGGCCAGCCCTGGAAGAAGGGCTCCACCGAGAAGGCGGCGCACCCGAACA
GCCGCTTCACCGCGCCGGCGAAGAACAACCCCGCCATCTCGCCGCTGGTGGACGATCCGAAGGGCGTGCCCATCTCGGCC
ATCATCTTCGGCGGGCGGCGCTCCACCACGGTCCCGCTGGTGCTCGAGGCGTTCAACTGGACGCACGGCGTGTACCTGGG
CTCGACCATGGGCTCGGAGACCACCGCCGCCGCCACCGGCCAGGTCGGCGTGGTCCGCCGCGACCCGATGGCGATGCTGC
CGTTCATCGGCTACGACTGCGGGAGCTACCTGCAGCACTGGCTCGACATGCAGTCGCGCATCCCGAACCCGCCCAAGATC
TTCCTGGTGAACTGGTTCCGGAAGAGCGCCGAGGGCAAGTTCCTGTGGCCGGGCTACGGCGACAACATGCGCGTGCTGAA
GTGGATGCTCGACCGCGCCGCCGGCCGCGCGCCCGCCAAGGAGACGCTGCTCGGGTACACACCGGGTGACTCGGGCCTCG
ACCTGCACGGCCTCGACGTCTCGAAGGACGCGATCGCGGCCGCGACCCAGATCGACCTCGGCGAGTGGGAGCAGGAGCTG
GAGTCGCAGTCCGAGTGGTTCGAGAAGCTCGGCAAGACGCTGCCCCGGCCGCTCGCGCTCCAGCGCGAGCTGCTGCTCGA
GCGCGTCCGCGCCGCCCGCAAGGTGAAGTAG

Upstream 100 bases:

>100_bases
TCTGCGACCTCTCGTGCGCTAGCTTGGCGTCCCCGTTTTCCGCGCTGCAGCTGCGCGTCCCCAGGCCCTTTTTCCGCTCA
ATCGTAAAAAGGAATTCCTG

Downstream 100 bases:

>100_bases
CGCGCGCCGCGCGAGACGTTCCGGGGGCCCGGCCGGCGACGGTCGGGCCCCTTCCCGTCGCGCCGGGCGAGCGGGACCGC
CGGCCCCGTGTTAAGCGTCC

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase

Number of amino acids: Translated: 596; Mature: 595

Protein sequence:

>596_residues
MSTSPAARPTSNAHLLGWVDEMAKLCKPDRVYWCDGSEAEKKRLTDDAVAAKVLIPLDQQKWPGCHYHHSNSSDVARVEH
LTFICTPTKEQAGPTNNWMEPKEAYRKLGAIFDGSMKGRTMYVVPYVMGPSTSPFAKVGIEITDSVYVALNMGIMARMGK
VALDRLGDSDEFNRGLHSVADCNPERRFICHFPQDNTIWSVGSGYGGNALLGKKCLALRIASYLAKNEGWLAEHMLILEA
ESPTGEKQYVAAAFPSACGKTNFAMMIPPAAFPGWKIRTVGDDISWMRVGEDGRLWAVNPENGYFGVAPGTNRKTNPNAM
DSVRKDTIFTNVARTPDGDIWWEGMDHEAPAELIDWKGQPWKKGSTEKAAHPNSRFTAPAKNNPAISPLVDDPKGVPISA
IIFGGRRSTTVPLVLEAFNWTHGVYLGSTMGSETTAAATGQVGVVRRDPMAMLPFIGYDCGSYLQHWLDMQSRIPNPPKI
FLVNWFRKSAEGKFLWPGYGDNMRVLKWMLDRAAGRAPAKETLLGYTPGDSGLDLHGLDVSKDAIAAATQIDLGEWEQEL
ESQSEWFEKLGKTLPRPLALQRELLLERVRAARKVK

Sequences:

>Translated_596_residues
MSTSPAARPTSNAHLLGWVDEMAKLCKPDRVYWCDGSEAEKKRLTDDAVAAKVLIPLDQQKWPGCHYHHSNSSDVARVEH
LTFICTPTKEQAGPTNNWMEPKEAYRKLGAIFDGSMKGRTMYVVPYVMGPSTSPFAKVGIEITDSVYVALNMGIMARMGK
VALDRLGDSDEFNRGLHSVADCNPERRFICHFPQDNTIWSVGSGYGGNALLGKKCLALRIASYLAKNEGWLAEHMLILEA
ESPTGEKQYVAAAFPSACGKTNFAMMIPPAAFPGWKIRTVGDDISWMRVGEDGRLWAVNPENGYFGVAPGTNRKTNPNAM
DSVRKDTIFTNVARTPDGDIWWEGMDHEAPAELIDWKGQPWKKGSTEKAAHPNSRFTAPAKNNPAISPLVDDPKGVPISA
IIFGGRRSTTVPLVLEAFNWTHGVYLGSTMGSETTAAATGQVGVVRRDPMAMLPFIGYDCGSYLQHWLDMQSRIPNPPKI
FLVNWFRKSAEGKFLWPGYGDNMRVLKWMLDRAAGRAPAKETLLGYTPGDSGLDLHGLDVSKDAIAAATQIDLGEWEQEL
ESQSEWFEKLGKTLPRPLALQRELLLERVRAARKVK
>Mature_595_residues
STSPAARPTSNAHLLGWVDEMAKLCKPDRVYWCDGSEAEKKRLTDDAVAAKVLIPLDQQKWPGCHYHHSNSSDVARVEHL
TFICTPTKEQAGPTNNWMEPKEAYRKLGAIFDGSMKGRTMYVVPYVMGPSTSPFAKVGIEITDSVYVALNMGIMARMGKV
ALDRLGDSDEFNRGLHSVADCNPERRFICHFPQDNTIWSVGSGYGGNALLGKKCLALRIASYLAKNEGWLAEHMLILEAE
SPTGEKQYVAAAFPSACGKTNFAMMIPPAAFPGWKIRTVGDDISWMRVGEDGRLWAVNPENGYFGVAPGTNRKTNPNAMD
SVRKDTIFTNVARTPDGDIWWEGMDHEAPAELIDWKGQPWKKGSTEKAAHPNSRFTAPAKNNPAISPLVDDPKGVPISAI
IFGGRRSTTVPLVLEAFNWTHGVYLGSTMGSETTAAATGQVGVVRRDPMAMLPFIGYDCGSYLQHWLDMQSRIPNPPKIF
LVNWFRKSAEGKFLWPGYGDNMRVLKWMLDRAAGRAPAKETLLGYTPGDSGLDLHGLDVSKDAIAAATQIDLGEWEQELE
SQSEWFEKLGKTLPRPLALQRELLLERVRAARKVK

Specific function: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle

COG id: COG1274

COG function: function code C; Phosphoenolpyruvate carboxykinase (GTP)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [GTP] family

Homologues:

Organism=Homo sapiens, GI66346721, Length=605, Percent_Identity=48.7603305785124, Blast_Score=561, Evalue=1e-160,
Organism=Homo sapiens, GI187281517, Length=601, Percent_Identity=47.9201331114809, Blast_Score=541, Evalue=1e-154,
Organism=Homo sapiens, GI66346723, Length=388, Percent_Identity=49.2268041237113, Blast_Score=361, Evalue=1e-99,
Organism=Caenorhabditis elegans, GI17555492, Length=611, Percent_Identity=46.6448445171849, Blast_Score=526, Evalue=1e-150,
Organism=Caenorhabditis elegans, GI71989645, Length=606, Percent_Identity=45.7095709570957, Blast_Score=521, Evalue=1e-148,
Organism=Caenorhabditis elegans, GI71989658, Length=607, Percent_Identity=45.6342668863262, Blast_Score=521, Evalue=1e-148,
Organism=Caenorhabditis elegans, GI71989653, Length=593, Percent_Identity=46.0370994940978, Blast_Score=515, Evalue=1e-146,
Organism=Caenorhabditis elegans, GI32564411, Length=532, Percent_Identity=50, Blast_Score=509, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI32564407, Length=523, Percent_Identity=49.5219885277247, Blast_Score=496, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI71989649, Length=529, Percent_Identity=46.5028355387524, Blast_Score=455, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI32564409, Length=365, Percent_Identity=49.041095890411, Blast_Score=347, Evalue=9e-96,
Organism=Caenorhabditis elegans, GI17540956, Length=227, Percent_Identity=27.7533039647577, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24655085, Length=598, Percent_Identity=50.3344481605351, Blast_Score=573, Evalue=1e-164,
Organism=Drosophila melanogaster, GI24655082, Length=599, Percent_Identity=49.2487479131886, Blast_Score=551, Evalue=1e-157,
Organism=Drosophila melanogaster, GI161077226, Length=564, Percent_Identity=50, Blast_Score=521, Evalue=1e-148,
Organism=Drosophila melanogaster, GI24655088, Length=495, Percent_Identity=53.1313131313131, Blast_Score=509, Evalue=1e-144,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PCKG_ANAD2 (B8J7G1)

Other databases:

- EMBL:   CP001359
- RefSeq:   YP_002494207.1
- ProteinModelPortal:   B8J7G1
- SMR:   B8J7G1
- GeneID:   7296193
- GenomeReviews:   CP001359_GR
- KEGG:   acp:A2cp1_3817
- HOGENOM:   HBG484437
- ProtClustDB:   PRK04210
- GO:   GO:0005737
- GO:   GO:0006094
- HAMAP:   MF_00452
- InterPro:   IPR018091
- InterPro:   IPR013035
- InterPro:   IPR008209
- InterPro:   IPR008210
- Gene3D:   G3DSA:3.90.228.20
- Gene3D:   G3DSA:3.40.449.10
- PANTHER:   PTHR11561
- PIRSF:   PIRSF001348

Pfam domain/function: PF00821 PEPCK; SSF68923 PEP_carboxykinase_N

EC number: =4.1.1.32

Molecular weight: Translated: 65870; Mature: 65739

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: PS00505 PEPCK_GTP

Important sites: ACT_SITE 258-258 BINDING 77-77 BINDING 207-207 BINDING 214-214 BINDING 256-256 BINDING 375-375 BINDING 406-406

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSPAARPTSNAHLLGWVDEMAKLCKPDRVYWCDGSEAEKKRLTDDAVAAKVLIPLDQQ
CCCCCCCCCCCCCEEEEHHHHHHHHCCCCEEEEECCCHHHHHHCCHHHHEEEEEEECCCC
KWPGCHYHHSNSSDVARVEHLTFICTPTKEQAGPTNNWMEPKEAYRKLGAIFDGSMKGRT
CCCCCCCCCCCCCCCEEEEEEEEEECCCHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCE
MYVVPYVMGPSTSPFAKVGIEITDSVYVALNMGIMARMGKVALDRLGDSDEFNRGLHSVA
EEEEEEEECCCCCCHHEECEEECCCEEEEECHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
DCNPERRFICHFPQDNTIWSVGSGYGGNALLGKKCLALRIASYLAKNEGWLAEHMLILEA
CCCCCCEEEEECCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEC
ESPTGEKQYVAAAFPSACGKTNFAMMIPPAAFPGWKIRTVGDDISWMRVGEDGRLWAVNP
CCCCCCCEEEEECCCHHCCCCCEEEEECCCCCCCCEEEECCCCCEEEEECCCCCEEEECC
ENGYFGVAPGTNRKTNPNAMDSVRKDTIFTNVARTPDGDIWWEGMDHEAPAELIDWKGQP
CCCEEECCCCCCCCCCCHHHHHHHHHHHHEECCCCCCCCEEECCCCCCCCHHHHCCCCCC
WKKGSTEKAAHPNSRFTAPAKNNPAISPLVDDPKGVPISAIIFGGRRSTTVPLVLEAFNW
CCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEECCC
THGVYLGSTMGSETTAAATGQVGVVRRDPMAMLPFIGYDCGSYLQHWLDMQSRIPNPPKI
CCCEEEECCCCCCCCEECCCCEEEEECCCHHHCCHHCCCHHHHHHHHHHHHHHCCCCCEE
FLVNWFRKSAEGKFLWPGYGDNMRVLKWMLDRAAGRAPAKETLLGYTPGDSGLDLHGLDV
EEEEEEHHCCCCCEECCCCCCCHHHHHHHHHHHCCCCCCHHCEEEECCCCCCCEEECCCC
SKDAIAAATQIDLGEWEQELESQSEWFEKLGKTLPRPLALQRELLLERVRAARKVK
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
STSPAARPTSNAHLLGWVDEMAKLCKPDRVYWCDGSEAEKKRLTDDAVAAKVLIPLDQQ
CCCCCCCCCCCCEEEEHHHHHHHHCCCCEEEEECCCHHHHHHCCHHHHEEEEEEECCCC
KWPGCHYHHSNSSDVARVEHLTFICTPTKEQAGPTNNWMEPKEAYRKLGAIFDGSMKGRT
CCCCCCCCCCCCCCCEEEEEEEEEECCCHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCE
MYVVPYVMGPSTSPFAKVGIEITDSVYVALNMGIMARMGKVALDRLGDSDEFNRGLHSVA
EEEEEEEECCCCCCHHEECEEECCCEEEEECHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
DCNPERRFICHFPQDNTIWSVGSGYGGNALLGKKCLALRIASYLAKNEGWLAEHMLILEA
CCCCCCEEEEECCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEC
ESPTGEKQYVAAAFPSACGKTNFAMMIPPAAFPGWKIRTVGDDISWMRVGEDGRLWAVNP
CCCCCCCEEEEECCCHHCCCCCEEEEECCCCCCCCEEEECCCCCEEEEECCCCCEEEECC
ENGYFGVAPGTNRKTNPNAMDSVRKDTIFTNVARTPDGDIWWEGMDHEAPAELIDWKGQP
CCCEEECCCCCCCCCCCHHHHHHHHHHHHEECCCCCCCCEEECCCCCCCCHHHHCCCCCC
WKKGSTEKAAHPNSRFTAPAKNNPAISPLVDDPKGVPISAIIFGGRRSTTVPLVLEAFNW
CCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEECCC
THGVYLGSTMGSETTAAATGQVGVVRRDPMAMLPFIGYDCGSYLQHWLDMQSRIPNPPKI
CCCEEEECCCCCCCCEECCCCEEEEECCCHHHCCHHCCCHHHHHHHHHHHHHHCCCCCEE
FLVNWFRKSAEGKFLWPGYGDNMRVLKWMLDRAAGRAPAKETLLGYTPGDSGLDLHGLDV
EEEEEEHHCCCCCEECCCCCCCHHHHHHHHHHHCCCCCCHHCEEEECCCCCCCEEECCCC
SKDAIAAATQIDLGEWEQELESQSEWFEKLGKTLPRPLALQRELLLERVRAARKVK
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA