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The map label for this gene is truD

Identifier: 220918870

GI number: 220918870

Start: 4203611

End: 4204657

Strand: Direct

Name: truD

Synonym: A2cp1_3784

Alternate gene names: 220918870

Gene position: 4203611-4204657 (Clockwise)

Preceding gene: 220918869

Following gene: 220918873

Centisome position: 83.58

GC content: 78.22

Gene sequence:

>1047_bases
ATGACCGACCTCCCCTTCGTCACCGCCGACCTGCCCGGCTCCGGAGGCGCGCTGCGCCGCGCCCCCGAGGACTTCCGGGT
GGACGAGGTGCCCGCCTACCTGCCGTCCGGCGCGGGCCCGCACCTGTACCTCCGCGTGGAGAAGCGCGGGCGCACCTCCC
GCGACGCGGTGCGCGAGCTGGCGCGGGCGCTGGGCGTGCCCGAGCGCGACGCCGGCTGCGCCGGGCTGAAGGACAAGGAC
GCGGTCACCACGCAGTGGCTCTCGTTCCCGGTGGCGCGCGATCCCGACCCGGCCGCGCTCGCCGCGCCGGGCCTGCGCGT
GCTGGAGGCCTCGCGCCACCAGAACAAGCTCCGCACCGGCCACGTGCGCGCGAACCGCTTCACGCTGGCGGTGCGCGGCG
GCGATCTGGGGCGGGCCCGGGAGTGCGCCGGCGCGCTCGCGGCGCGGGGGCTCCCCAACTTCTTCGGGCCGCAGCGCTTC
GGCGCCGCCGGGCGCAACGCGGCGGTGGGGCGCGCGCTCGTCACCGGCGAGCGGACCCCGGAGGCCGGCCGCGCCGCGCG
GGATCGCTTCCTGCGCCGGCTCTCGCTCTCCGCCTACCAGTCGCTCCTGTTCAATCGCTGGCTGGCGGAGCGGATGGCGG
ACGGGCTGTTCGCGGCGGCGCTGGCCGGCGACGCGATGAAGAAGCTCGACACCGGCGGGCTGTTCACCTGCGAGGACCCC
GCCGCGGACGGGCCGCGGGTGGAGCGGTTCGAGATCTCGCCGGCCGGCCCGATGTTCGGGCACGCGCTGCGCCTGGCGGC
CGGCGAGGCCGGGGCCCGCGAGGCGCGGCTGCTCGAGGCCGAGGGGATCGCGCTCGCCGACTTCGCGCGCGGCGGCGGCG
AGGCCGAGGGCACCCGCCGCGCGGCGCGGCTGCGGGTGGAGGTCGCGCTCGAGCCGCTGGAGGACGGGTACCGGGCGGCG
TTCGAGCTGCCCCGCGGCGCGTACGCCACCGTGGTCATGCGCGAGCTGACCAAGGGCGAGGCGGAGCTGCCCGAGGACGC
AGACTGA

Upstream 100 bases:

>100_bases
TCTTCGCCGCCGGCGACCGCCTGGAGCGCGCGGCGGAGCGCGCCCTGGGGGCGTCGCTCCGGCGCGGCCTCGCGCGCGTC
GAGGCGCTCCTCCCCTCCGC

Downstream 100 bases:

>100_bases
TCAGGCGCCCAGGCGCGCCGCCGCGTGGTCCGCGAGGAGCGCCAGCACCGCCACGTCCTGCTGGTCGAACAGCTCGCCGC
CCCGCTTGTTCATCACCTCC

Product: tRNA pseudouridine synthase D TruD

Products: NA

Alternate protein names: tRNA pseudouridylate synthase D; tRNA-uridine isomerase D

Number of amino acids: Translated: 348; Mature: 347

Protein sequence:

>348_residues
MTDLPFVTADLPGSGGALRRAPEDFRVDEVPAYLPSGAGPHLYLRVEKRGRTSRDAVRELARALGVPERDAGCAGLKDKD
AVTTQWLSFPVARDPDPAALAAPGLRVLEASRHQNKLRTGHVRANRFTLAVRGGDLGRARECAGALAARGLPNFFGPQRF
GAAGRNAAVGRALVTGERTPEAGRAARDRFLRRLSLSAYQSLLFNRWLAERMADGLFAAALAGDAMKKLDTGGLFTCEDP
AADGPRVERFEISPAGPMFGHALRLAAGEAGAREARLLEAEGIALADFARGGGEAEGTRRAARLRVEVALEPLEDGYRAA
FELPRGAYATVVMRELTKGEAELPEDAD

Sequences:

>Translated_348_residues
MTDLPFVTADLPGSGGALRRAPEDFRVDEVPAYLPSGAGPHLYLRVEKRGRTSRDAVRELARALGVPERDAGCAGLKDKD
AVTTQWLSFPVARDPDPAALAAPGLRVLEASRHQNKLRTGHVRANRFTLAVRGGDLGRARECAGALAARGLPNFFGPQRF
GAAGRNAAVGRALVTGERTPEAGRAARDRFLRRLSLSAYQSLLFNRWLAERMADGLFAAALAGDAMKKLDTGGLFTCEDP
AADGPRVERFEISPAGPMFGHALRLAAGEAGAREARLLEAEGIALADFARGGGEAEGTRRAARLRVEVALEPLEDGYRAA
FELPRGAYATVVMRELTKGEAELPEDAD
>Mature_347_residues
TDLPFVTADLPGSGGALRRAPEDFRVDEVPAYLPSGAGPHLYLRVEKRGRTSRDAVRELARALGVPERDAGCAGLKDKDA
VTTQWLSFPVARDPDPAALAAPGLRVLEASRHQNKLRTGHVRANRFTLAVRGGDLGRARECAGALAARGLPNFFGPQRFG
AAGRNAAVGRALVTGERTPEAGRAARDRFLRRLSLSAYQSLLFNRWLAERMADGLFAAALAGDAMKKLDTGGLFTCEDPA
ADGPRVERFEISPAGPMFGHALRLAAGEAGAREARLLEAEGIALADFARGGGEAEGTRRAARLRVEVALEPLEDGYRAAF
ELPRGAYATVVMRELTKGEAELPEDAD

Specific function: Responsible for synthesis of pseudouridine from uracil- 13 in transfer RNAs

COG id: COG0585

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRUD domain

Homologues:

Organism=Escherichia coli, GI1789102, Length=334, Percent_Identity=34.1317365269461, Blast_Score=141, Evalue=5e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUD_ANAD2 (B8J7C8)

Other databases:

- EMBL:   CP001359
- RefSeq:   YP_002494174.1
- ProteinModelPortal:   B8J7C8
- SMR:   B8J7C8
- GeneID:   7296160
- GenomeReviews:   CP001359_GR
- KEGG:   acp:A2cp1_3784
- HOGENOM:   HBG541221
- ProtClustDB:   CLSK946782
- HAMAP:   MF_01082
- InterPro:   IPR020103
- InterPro:   IPR001656
- InterPro:   IPR020119
- InterPro:   IPR011760
- PANTHER:   PTHR13326

Pfam domain/function: PF01142 TruD; SSF55120 PsdUridine_synth_cat_dom

EC number: NA

Molecular weight: Translated: 37162; Mature: 37031

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: PS50984 TRUD; PS01268 UPF0024

Important sites: ACT_SITE 78-78

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLPFVTADLPGSGGALRRAPEDFRVDEVPAYLPSGAGPHLYLRVEKRGRTSRDAVREL
CCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHH
ARALGVPERDAGCAGLKDKDAVTTQWLSFPVARDPDPAALAAPGLRVLEASRHQNKLRTG
HHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHC
HVRANRFTLAVRGGDLGRARECAGALAARGLPNFFGPQRFGAAGRNAAVGRALVTGERTP
EEEEEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCHHHCCCCCCHHHHHHEECCCCCC
EAGRAARDRFLRRLSLSAYQSLLFNRWLAERMADGLFAAALAGDAMKKLDTGGLFTCEDP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCC
AADGPRVERFEISPAGPMFGHALRLAAGEAGAREARLLEAEGIALADFARGGGEAEGTRR
CCCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCHHEEECCCCCEEEHHHCCCCCCHHHHH
AARLRVEVALEPLEDGYRAAFELPRGAYATVVMRELTKGEAELPEDAD
HHHEEEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TDLPFVTADLPGSGGALRRAPEDFRVDEVPAYLPSGAGPHLYLRVEKRGRTSRDAVREL
CCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHH
ARALGVPERDAGCAGLKDKDAVTTQWLSFPVARDPDPAALAAPGLRVLEASRHQNKLRTG
HHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHC
HVRANRFTLAVRGGDLGRARECAGALAARGLPNFFGPQRFGAAGRNAAVGRALVTGERTP
EEEEEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCHHHCCCCCCHHHHHHEECCCCCC
EAGRAARDRFLRRLSLSAYQSLLFNRWLAERMADGLFAAALAGDAMKKLDTGGLFTCEDP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCC
AADGPRVERFEISPAGPMFGHALRLAAGEAGAREARLLEAEGIALADFARGGGEAEGTRR
CCCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCHHEEECCCCCEEEHHHCCCCCCHHHHH
AARLRVEVALEPLEDGYRAAFELPRGAYATVVMRELTKGEAELPEDAD
HHHEEEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA