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The map label for this gene is prfA [H]

Identifier: 220918650

GI number: 220918650

Start: 3976061

End: 3976432

Strand: Reverse

Name: prfA [H]

Synonym: A2cp1_3558

Alternate gene names: 220918650

Gene position: 3976432-3976061 (Counterclockwise)

Preceding gene: 220918651

Following gene: 220918647

Centisome position: 79.06

GC content: 78.23

Gene sequence:

>372_bases
ATGACGGTCCCCGAGGCGCTGCGGGCCGAGGCCCGCCGGGCGCTCGCCCTGCCCGACGAGGCGCTGCTCGCCGAGTGCGA
CGAGAGCTTCTTCGTGGGCGGCGGGCCCGGCGGCCAGCACCGCAACAAGACCGAGAGCGGCGTGCGGCTGGTGCACCGGC
CCACCGCGATCACGGTGACCGCCACCGAGCGGCGCAGCCAGCTGCAGAACCGCGGCGCGGCGCTGGAGCGGCTGCGCGCG
CGCCTCCAGCCGCTCGCGCACCGGCCCAAGCCGCGGCGGCCCACCAGACCGACGCGCGGCGCGAAGGAGCGGCGGCTCAC
CGAGAAGAAGCGCCGCGGCGAGCGGAAGGCCTCGCGGCGCGGGTGGGAGTGA

Upstream 100 bases:

>100_bases
TCGTGACGCACGACCCGCGGCTCGCCGACCGCTGCGATCGCATCATCGAGCTGGTGGACGGGCGCATCGTGGCCGACGGG
CCCCACCGGAGGAGCGGAGC

Downstream 100 bases:

>100_bases
CGCCGGGGGCCGCTACCACCGGCCGGTGACGGGCGGAGGCGCGCGCTGCGCCTCGAGCTGCGAGGCGAGCGGGACCAGCT
CGGCGAGGCCGGCGCGGACC

Product: Class I peptide chain release factor

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 123; Mature: 122

Protein sequence:

>123_residues
MTVPEALRAEARRALALPDEALLAECDESFFVGGGPGGQHRNKTESGVRLVHRPTAITVTATERRSQLQNRGAALERLRA
RLQPLAHRPKPRRPTRPTRGAKERRLTEKKRRGERKASRRGWE

Sequences:

>Translated_123_residues
MTVPEALRAEARRALALPDEALLAECDESFFVGGGPGGQHRNKTESGVRLVHRPTAITVTATERRSQLQNRGAALERLRA
RLQPLAHRPKPRRPTRPTRGAKERRLTEKKRRGERKASRRGWE
>Mature_122_residues
TVPEALRAEARRALALPDEALLAECDESFFVGGGPGGQHRNKTESGVRLVHRPTAITVTATERRSQLQNRGAALERLRAR
LQPLAHRPKPRRPTRPTRGAKERRLTEKKRRGERKASRRGWE

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Escherichia coli, GI1786389, Length=131, Percent_Identity=35.8778625954198, Blast_Score=67, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 13918; Mature: 13787

Theoretical pI: Translated: 12.22; Mature: 12.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVPEALRAEARRALALPDEALLAECDESFFVGGGPGGQHRNKTESGVRLVHRPTAITVT
CCCCHHHHHHHHHHHCCCHHHHHHHCCCCEEECCCCCCCCCCCCCCCCEEEECCCEEEEE
ATERRSQLQNRGAALERLRARLQPLAHRPKPRRPTRPTRGAKERRLTEKKRRGERKASRR
HHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
GWE
CCC
>Mature Secondary Structure 
TVPEALRAEARRALALPDEALLAECDESFFVGGGPGGQHRNKTESGVRLVHRPTAITVT
CCCHHHHHHHHHHHCCCHHHHHHHCCCCEEECCCCCCCCCCCCCCCCEEEECCCEEEEE
ATERRSQLQNRGAALERLRARLQPLAHRPKPRRPTRPTRGAKERRLTEKKRRGERKASRR
HHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
GWE
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA