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The map label for this gene is cls [H]

Identifier: 220918608

GI number: 220918608

Start: 3930194

End: 3931447

Strand: Direct

Name: cls [H]

Synonym: A2cp1_3516

Alternate gene names: 220918608

Gene position: 3930194-3931447 (Clockwise)

Preceding gene: 220918593

Following gene: 220918611

Centisome position: 78.15

GC content: 75.68

Gene sequence:

>1254_bases
ATGCGCCTGAGGACGGACGGAGTGCTGGCGAGCGCCTTCCCGCTGGCGCTCGGGGTGGCCCTGGGCGGCCATCTCTCCCG
CCGGCTGCGCCGGCAGCAGCTCGCCCGGCGGTTCATCCGCCTGGCGGACGAGGTGCCGCTCGACGTGCTGCTGGAGCGCG
AGGTGGGCGTACCGGTCCGGGAGGCGAACGAGGTCTCGCTGCTCGAGGACGGGTCGATCTTCGACGCGCTGGAGCGCGAG
ATCCGGTCCGCGCGCGAGTCGATCCACATCTGCGAGTTCATGTGGCGCGGCACCGGCGACCCCTCCGAGCGGATCGGCCG
CGCGGTGCTCGAGCGGCGCCCCGGCGTCGTCTGCCGGATCGTGCTCGACTGGTTCGGCTCGCAGCACTTCGACCCGGCGC
TGGAGCGGCGGCTGGTGGCGAGCGGCTGCGCCATCCGGCGCCACGCGCCGCTGCCGGACCCGTTCCGCTGCAACCACCGG
CGCGTGCTGGTGTTCGACGGGCGGACCGCGTTCGTGGGCGGCTTCGGGATCTGGAAGAGCTGGCTCGGGAACGGGGTGGG
CGAGGAGGGCTGGCGCGACACGGCCGCCCGGGTGCGCGGCCCCGCGGTGGGCGACCTGCAGCGCGCCTTCGACGAGAGCT
TCCAGGCGGCGGGCGGCCAGCCGCTCCCGGCCTCCGCCTACCCGCGCCTCGCGCCGGCCGGGCCGCACCGCGTGGCGGTG
GTCGCGTCCACGCCCGCCTGGGCGCGCGCCACGCTGGCCGCGCGGATGAGCTATGCGCTGCTGGCCTCGGCCCGGCGCCG
GCTGTGGATCGCGAACTCCTACTTCGTGCCGGATCGCTCGCTCCAGGACGTGCTGATCGCCCGTGCCCGGGCCGGGGTGG
ACGTGCGGGTGCTCGCGCCGGGACCGTGGCACGACGTTCCGCTGGTGCAGGCCGGGCAGCGCCGGACCTACCGGCGGCTG
CTCGCGGGCGGGGTGCGCGTGTTCGAGTACGACGCGTCGATGATGCACGCGAAGACCATCCTCGCGGACGGCGCCTGCGC
GATCGGCTCGACCAACATGGACTCGCAGAGCCTCTCGTTCCTGTGGGAGACGTCGATCGTGACCGACGCGCCCGAGGCGG
CCGCCGGGCTCGCCCGGCGGTTCGGGCTGGACCTGACCCGCTCGTACGAGATCGAGCCCGGCGCCTGGCGGCGGCGGCCG
AGGGCCATGCGGCTCCGCGAGGCCGGCGCCGCGGTGGCCGAGCCCTGGCTCTGA

Upstream 100 bases:

>100_bases
CACCACTCCTCGTCCGGCGCCGCCCCGCTGCGACATGCCGGCAAGGTCGTCATCGCGGGAGCGCGGGCCCGGACCGCGGC
CCCGTGCCCAACCTTCGGGC

Downstream 100 bases:

>100_bases
GCTGCGCGGCGGCTCCGGACGCACGACGGCCGGCGCCGCGTCGCCGCGGGCCGGCCGTGGAGGTCCCGGAGGACGGGGCG
GCTAGCGCTTGGAGAACTGG

Product: phospholipase D/Transphosphatidylase

Products: cardiolipin; glycerol [C]

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MRLRTDGVLASAFPLALGVALGGHLSRRLRRQQLARRFIRLADEVPLDVLLEREVGVPVREANEVSLLEDGSIFDALERE
IRSARESIHICEFMWRGTGDPSERIGRAVLERRPGVVCRIVLDWFGSQHFDPALERRLVASGCAIRRHAPLPDPFRCNHR
RVLVFDGRTAFVGGFGIWKSWLGNGVGEEGWRDTAARVRGPAVGDLQRAFDESFQAAGGQPLPASAYPRLAPAGPHRVAV
VASTPAWARATLAARMSYALLASARRRLWIANSYFVPDRSLQDVLIARARAGVDVRVLAPGPWHDVPLVQAGQRRTYRRL
LAGGVRVFEYDASMMHAKTILADGACAIGSTNMDSQSLSFLWETSIVTDAPEAAAGLARRFGLDLTRSYEIEPGAWRRRP
RAMRLREAGAAVAEPWL

Sequences:

>Translated_417_residues
MRLRTDGVLASAFPLALGVALGGHLSRRLRRQQLARRFIRLADEVPLDVLLEREVGVPVREANEVSLLEDGSIFDALERE
IRSARESIHICEFMWRGTGDPSERIGRAVLERRPGVVCRIVLDWFGSQHFDPALERRLVASGCAIRRHAPLPDPFRCNHR
RVLVFDGRTAFVGGFGIWKSWLGNGVGEEGWRDTAARVRGPAVGDLQRAFDESFQAAGGQPLPASAYPRLAPAGPHRVAV
VASTPAWARATLAARMSYALLASARRRLWIANSYFVPDRSLQDVLIARARAGVDVRVLAPGPWHDVPLVQAGQRRTYRRL
LAGGVRVFEYDASMMHAKTILADGACAIGSTNMDSQSLSFLWETSIVTDAPEAAAGLARRFGLDLTRSYEIEPGAWRRRP
RAMRLREAGAAVAEPWL
>Mature_417_residues
MRLRTDGVLASAFPLALGVALGGHLSRRLRRQQLARRFIRLADEVPLDVLLEREVGVPVREANEVSLLEDGSIFDALERE
IRSARESIHICEFMWRGTGDPSERIGRAVLERRPGVVCRIVLDWFGSQHFDPALERRLVASGCAIRRHAPLPDPFRCNHR
RVLVFDGRTAFVGGFGIWKSWLGNGVGEEGWRDTAARVRGPAVGDLQRAFDESFQAAGGQPLPASAYPRLAPAGPHRVAV
VASTPAWARATLAARMSYALLASARRRLWIANSYFVPDRSLQDVLIARARAGVDVRVLAPGPWHDVPLVQAGQRRTYRRL
LAGGVRVFEYDASMMHAKTILADGACAIGSTNMDSQSLSFLWETSIVTDAPEAAAGLARRFGLDLTRSYEIEPGAWRRRP
RAMRLREAGAAVAEPWL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787007, Length=328, Percent_Identity=29.8780487804878, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1787502, Length=377, Percent_Identity=25.7294429708223, Blast_Score=119, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 46089; Mature: 46089

Theoretical pI: Translated: 10.96; Mature: 10.96

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLRTDGVLASAFPLALGVALGGHLSRRLRRQQLARRFIRLADEVPLDVLLEREVGVPVR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEHHCCCCCCC
EANEVSLLEDGSIFDALEREIRSARESIHICEFMWRGTGDPSERIGRAVLERRPGVVCRI
CCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEH
VLDWFGSQHFDPALERRLVASGCAIRRHAPLPDPFRCNHRRVLVFDGRTAFVGGFGIWKS
HHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCEEEEECHHHHHH
WLGNGVGEEGWRDTAARVRGPAVGDLQRAFDESFQAAGGQPLPASAYPRLAPAGPHRVAV
HHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEE
VASTPAWARATLAARMSYALLASARRRLWIANSYFVPDRSLQDVLIARARAGVDVRVLAP
EECCCHHHHHHHHHHHHHHHHHHHHHHEEEECCEECCCCCHHHHHHHHHHCCCEEEEECC
GPWHDVPLVQAGQRRTYRRLLAGGVRVFEYDASMMHAKTILADGACAIGSTNMDSQSLSF
CCCCCCCHHHCCHHHHHHHHHHCCCEEEEECHHHHHHHHHHCCCCEECCCCCCCCHHHHH
LWETSIVTDAPEAAAGLARRFGLDLTRSYEIEPGAWRRRPRAMRLREAGAAVAEPWL
HEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHCCCHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MRLRTDGVLASAFPLALGVALGGHLSRRLRRQQLARRFIRLADEVPLDVLLEREVGVPVR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEHHCCCCCCC
EANEVSLLEDGSIFDALEREIRSARESIHICEFMWRGTGDPSERIGRAVLERRPGVVCRI
CCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEH
VLDWFGSQHFDPALERRLVASGCAIRRHAPLPDPFRCNHRRVLVFDGRTAFVGGFGIWKS
HHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCEEEEECHHHHHH
WLGNGVGEEGWRDTAARVRGPAVGDLQRAFDESFQAAGGQPLPASAYPRLAPAGPHRVAV
HHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEE
VASTPAWARATLAARMSYALLASARRRLWIANSYFVPDRSLQDVLIARARAGVDVRVLAP
EECCCHHHHHHHHHHHHHHHHHHHHHHEEEECCEECCCCCHHHHHHHHHHCCCEEEEECC
GPWHDVPLVQAGQRRTYRRLLAGGVRVFEYDASMMHAKTILADGACAIGSTNMDSQSLSF
CCCCCCCHHHCCHHHHHHHHHHCCCEEEEECHHHHHHHHHHCCCCEECCCCCCCCHHHHH
LWETSIVTDAPEAAAGLARRFGLDLTRSYEIEPGAWRRRPRAMRLREAGAAVAEPWL
HEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHCCCHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2 L-1-phosphatidyl-glycerol [C]

Specific reaction: 2 L-1-phosphatidyl-glycerol = cardiolipin + glycerol [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA