Click here to switch to the map view.

The map label for this gene is fliA [H]

Identifier: 220917697

GI number: 220917697

Start: 2877375

End: 2878073

Strand: Direct

Name: fliA [H]

Synonym: A2cp1_2598

Alternate gene names: 220917697

Gene position: 2877375-2878073 (Clockwise)

Preceding gene: 220917696

Following gene: 220917698

Centisome position: 57.21

GC content: 78.11

Gene sequence:

>699_bases
ATGCACGCCGCCCTCGCCGCCCTCGCCCGCTACGGTCCGCCCGCCCCCGAGCCGGACGACGCCCTGCTCCGGCGCCACGC
GCCGCTGCTCGATCGCTGCGCCCGCCGCCTCGCCGCCCGCACCGGCCATGCGGTGGAGCCCGGCGATCTCTGGTCCGCCG
GCGCCATGGGCTTGCTCGAGGCGGCGCGCCGCTACGACCCGGCGCACGACGTCCGCTTCGAGACCTTCGCCGAGCACCGG
GTGCGCGGCGCGATGCTGGACGAGCTGCGGCGGATGGACCACCTGCCGCGGCGCCTCCGCGCGGACGCCGACCGCGTGCT
CGCGGCCCGGGCCCGCCTGGAGCAGTCCCTCGGCCGCGAGCCCGACCCGGTGGAGGTGGCCGAGGCGGCGGAGCTCCCCG
CCGAGCAGGTGGGGGAGCTGCTCCTGCTCGCGTCGCCGCCCGTGCCGGTCGAGGACGACCTCGCGCCCACCCCCGCCCTG
CCCGCGGACGAGGCCCTCGGGGCGGCCGAGCGGCGCCGGGCCCTGGCGCGCATGGTGGCGGCGCTGCCGGAGCGGCTCCA
GGTGCTGCTGGCGCTCTACTACGACGAGGCGCTCACCTACCGGGAGATCGCGAAGGTGCTGGGCGTGTCCGAGCCGCGCG
TCTGCCAGCTCCACGCGGAGGCCGTGAAGAGGCTCCGCGCGCTCATGTCGGATACGTGA

Upstream 100 bases:

>100_bases
CGATCCTCTCGTCGCCGCCACCGGCCACCCTGCCCGGCGGCGTGAAGCTGTTCTGGCAGCAGCTCCTCCGCGAGCGGCAG
CCCGCCGCCTAGGTCCGCCG

Downstream 100 bases:

>100_bases
CGACATCCGACCACGTTTTCGGGTAGTACGCGGGCCTACATCGGTCCCAAGACATGCGGAACCCCGCGAAACGGGCGGCC
GTTCGCGGAAAGCTTTACCG

Product: RNA polymerase sigma-28 subunit FliA/WhiG

Products: NA

Alternate protein names: Sigma-28; Sigma-F factor [H]

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MHAALAALARYGPPAPEPDDALLRRHAPLLDRCARRLAARTGHAVEPGDLWSAGAMGLLEAARRYDPAHDVRFETFAEHR
VRGAMLDELRRMDHLPRRLRADADRVLAARARLEQSLGREPDPVEVAEAAELPAEQVGELLLLASPPVPVEDDLAPTPAL
PADEALGAAERRRALARMVAALPERLQVLLALYYDEALTYREIAKVLGVSEPRVCQLHAEAVKRLRALMSDT

Sequences:

>Translated_232_residues
MHAALAALARYGPPAPEPDDALLRRHAPLLDRCARRLAARTGHAVEPGDLWSAGAMGLLEAARRYDPAHDVRFETFAEHR
VRGAMLDELRRMDHLPRRLRADADRVLAARARLEQSLGREPDPVEVAEAAELPAEQVGELLLLASPPVPVEDDLAPTPAL
PADEALGAAERRRALARMVAALPERLQVLLALYYDEALTYREIAKVLGVSEPRVCQLHAEAVKRLRALMSDT
>Mature_232_residues
MHAALAALARYGPPAPEPDDALLRRHAPLLDRCARRLAARTGHAVEPGDLWSAGAMGLLEAARRYDPAHDVRFETFAEHR
VRGAMLDELRRMDHLPRRLRADADRVLAARARLEQSLGREPDPVEVAEAAELPAEQVGELLLLASPPVPVEDDLAPTPAL
PADEALGAAERRRALARMVAALPERLQVLLALYYDEALTYREIAKVLGVSEPRVCQLHAEAVKRLRALMSDT

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is specific for the flagellin gene (fliC) expression [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=220, Percent_Identity=38.1818181818182, Blast_Score=127, Evalue=7e-31,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 25505; Mature: 25505

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHAALAALARYGPPAPEPDDALLRRHAPLLDRCARRLAARTGHAVEPGDLWSAGAMGLLE
CHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
AARRYDPAHDVRFETFAEHRVRGAMLDELRRMDHLPRRLRADADRVLAARARLEQSLGRE
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PDPVEVAEAAELPAEQVGELLLLASPPVPVEDDLAPTPALPADEALGAAERRRALARMVA
CCCHHHHHHHCCCHHHHCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
ALPERLQVLLALYYDEALTYREIAKVLGVSEPRVCQLHAEAVKRLRALMSDT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHAALAALARYGPPAPEPDDALLRRHAPLLDRCARRLAARTGHAVEPGDLWSAGAMGLLE
CHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
AARRYDPAHDVRFETFAEHRVRGAMLDELRRMDHLPRRLRADADRVLAARARLEQSLGRE
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PDPVEVAEAAELPAEQVGELLLLASPPVPVEDDLAPTPALPADEALGAAERRRALARMVA
CCCHHHHHHHCCCHHHHCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
ALPERLQVLLALYYDEALTYREIAKVLGVSEPRVCQLHAEAVKRLRALMSDT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1560774; 10984043 [H]