Click here to switch to the map view.

The map label for this gene is hyfR [H]

Identifier: 220917651

GI number: 220917651

Start: 2828868

End: 2830445

Strand: Direct

Name: hyfR [H]

Synonym: A2cp1_2552

Alternate gene names: 220917651

Gene position: 2828868-2830445 (Clockwise)

Preceding gene: 220917646

Following gene: 220917653

Centisome position: 56.25

GC content: 74.65

Gene sequence:

>1578_bases
ATGGTTGGAACCGGGCTCCCCGTGGTTGGGCAGGACGACGTGCACCGCCGGATCGTCGAGGAGGCGATCGAGCTGGTGCT
CTCGACCGTCGATCTCCAGGCCGTGCTCGAGCGCACGGGGCGGCTCCTGCGCCGCCACTTCGGCGAGACGCGCGTGGCCA
TCAACCGCCTCTCGGCGGAGGACCCGACGCGCGCCGAGGTGGTGCTGGTCTCGGACCCCCGGCAACCCTCGCCCGAGGTG
GGCACGTCCTTCCCGCTGGCCGGCTCCGCCGCGGGGGAGGCGCTCGCGAAGCGGCTGCCCTGCGTGGTGGACCCGCTGCG
CCCGCGCGAGCCGCGCTGGCGCGAGGAGCCGCTGCTCGCCGCCTACGGCTACGGCTCGCTGGTCTCGTTCCCGCTGGTCT
TCGAGAACGAGCTGCTCGGGACGCTCGACATCGCGCACCCGCCGGCGGAGGGGCTGCTCGACTGCTGCTTCGAGGTGGCG
CGGCAGGTCGCGCACCTCGTCGCCATCGCGCTCCACAACAGCCTCATGGTGGAGGAGGTGCAGCGGCTGAACCGGCTCCT
CGGCCGCGAGAACGCCCTCCTCAAGGAGGAGATCCGCCAGATCAAGCGCGATTCCCGGTACGTGGCCGAGAGCGAGGGGA
TGCGCGCGGTGGTGGAGCGGGTGCGCCTGGTGGCGCCCTCCACCACCACCGTGCTGGTGCGCGGCGAGACCGGCACCGGC
AAGGAGGGGCTGGCGCGGATGGTCCACGAGTTCAGCCCGCGGTTCAACGCGCCGTTCGTGGCCGCGAACCTCGGCGCCAT
CCCCGAGGGGCTCATCGAGAGCGAGCTGTTCGGCCACGAGAAAGGCGCGTTCACCGGCGCCACCCGCCGCCGCGCCGGCC
GCTTCGAGCAGGCCGACGGCGGCACGCTGTTCCTCGACGAGGTCGGCGACGCGCCGCCCAGCGTGCAGGTGCGGCTGCTG
CGGGTGCTGCAGGAGCGCGTGGTGGAGCGGGTGGGCGGCACCGAGCCGGTCAAGGTGGACGTGCGCGTCATCGCCGCGAC
CAACCGCAACCTGGAGGAGATGGTCGCGCGCGGCACGTTCCGCGCCGACCTCTACTACCGGCTGGCCGTGTTCCCGATCG
AGCTGCCGCCGCTGCGCGAGCGGCGCGAGGACGTCCGGCCGCTCGGGACGTACTTCCTCGCGAAGCAGGCCGCGCTCATG
CACCGGCGCCCGCCGCGCGTGGGCGAGGACGTGTGGCGCGCGCTCGAGGCGTACGGCTGGCCCGGCAACGTGCGCGAGCT
GGAGAACTTCCTCCAGCGCGCGCTGATCCTCTCGCCCGGCCAGGAGCTCTCGCTGCCGGCGCTGCCCACCGCCGCGACGC
AGGCGTCCCCCGCCGCGGCGGCCGCCGCCGAGCCCGCCCCGCCCGGCCGCTTCGACGACGAGGTCCGCGAGCTGATCGAG
CGGGCGCTGGAGCACGCCGGGGGGCGGGTGTACGGCCCGGGCGGCGCCGCGGCGCTGCTCGGCCTGCGCCCCACCACGCT
GCAGGGGAAGATGAAGAAGTACGGGGTGGGCAACCCGGGCCGGCCGACCGGCACGTAG

Upstream 100 bases:

>100_bases
ACTCGACATTTCGGTAACCACTCAGGTTTTTCGGCTAGCGCCCCCTGAGGCACCGATCGACCGTTGCCTCCGGCACCGAT
ATTTCGGTAGACACCGTTCC

Downstream 100 bases:

>100_bases
CCCCGGGGCCGGCGGGCGGCTACCGCGCCCCGCGCCCGCTCGTGATCCGGAAGATCTCGGCGATGACGCCGCCGGCGGTG
TTCGCGCCGCCCACGCCCGC

Product: Fis Family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 525; Mature: 525

Protein sequence:

>525_residues
MVGTGLPVVGQDDVHRRIVEEAIELVLSTVDLQAVLERTGRLLRRHFGETRVAINRLSAEDPTRAEVVLVSDPRQPSPEV
GTSFPLAGSAAGEALAKRLPCVVDPLRPREPRWREEPLLAAYGYGSLVSFPLVFENELLGTLDIAHPPAEGLLDCCFEVA
RQVAHLVAIALHNSLMVEEVQRLNRLLGRENALLKEEIRQIKRDSRYVAESEGMRAVVERVRLVAPSTTTVLVRGETGTG
KEGLARMVHEFSPRFNAPFVAANLGAIPEGLIESELFGHEKGAFTGATRRRAGRFEQADGGTLFLDEVGDAPPSVQVRLL
RVLQERVVERVGGTEPVKVDVRVIAATNRNLEEMVARGTFRADLYYRLAVFPIELPPLRERREDVRPLGTYFLAKQAALM
HRRPPRVGEDVWRALEAYGWPGNVRELENFLQRALILSPGQELSLPALPTAATQASPAAAAAAEPAPPGRFDDEVRELIE
RALEHAGGRVYGPGGAAALLGLRPTTLQGKMKKYGVGNPGRPTGT

Sequences:

>Translated_525_residues
MVGTGLPVVGQDDVHRRIVEEAIELVLSTVDLQAVLERTGRLLRRHFGETRVAINRLSAEDPTRAEVVLVSDPRQPSPEV
GTSFPLAGSAAGEALAKRLPCVVDPLRPREPRWREEPLLAAYGYGSLVSFPLVFENELLGTLDIAHPPAEGLLDCCFEVA
RQVAHLVAIALHNSLMVEEVQRLNRLLGRENALLKEEIRQIKRDSRYVAESEGMRAVVERVRLVAPSTTTVLVRGETGTG
KEGLARMVHEFSPRFNAPFVAANLGAIPEGLIESELFGHEKGAFTGATRRRAGRFEQADGGTLFLDEVGDAPPSVQVRLL
RVLQERVVERVGGTEPVKVDVRVIAATNRNLEEMVARGTFRADLYYRLAVFPIELPPLRERREDVRPLGTYFLAKQAALM
HRRPPRVGEDVWRALEAYGWPGNVRELENFLQRALILSPGQELSLPALPTAATQASPAAAAAAEPAPPGRFDDEVRELIE
RALEHAGGRVYGPGGAAALLGLRPTTLQGKMKKYGVGNPGRPTGT
>Mature_525_residues
MVGTGLPVVGQDDVHRRIVEEAIELVLSTVDLQAVLERTGRLLRRHFGETRVAINRLSAEDPTRAEVVLVSDPRQPSPEV
GTSFPLAGSAAGEALAKRLPCVVDPLRPREPRWREEPLLAAYGYGSLVSFPLVFENELLGTLDIAHPPAEGLLDCCFEVA
RQVAHLVAIALHNSLMVEEVQRLNRLLGRENALLKEEIRQIKRDSRYVAESEGMRAVVERVRLVAPSTTTVLVRGETGTG
KEGLARMVHEFSPRFNAPFVAANLGAIPEGLIESELFGHEKGAFTGATRRRAGRFEQADGGTLFLDEVGDAPPSVQVRLL
RVLQERVVERVGGTEPVKVDVRVIAATNRNLEEMVARGTFRADLYYRLAVFPIELPPLRERREDVRPLGTYFLAKQAALM
HRRPPRVGEDVWRALEAYGWPGNVRELENFLQRALILSPGQELSLPALPTAATQASPAAAAAAEPAPPGRFDDEVRELIE
RALEHAGGRVYGPGGAAALLGLRPTTLQGKMKKYGVGNPGRPTGT

Specific function: Required for induction of expression of the hydrogenase- 4 structural genes [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87082117, Length=543, Percent_Identity=37.7532228360958, Blast_Score=327, Evalue=1e-90,
Organism=Escherichia coli, GI1789087, Length=396, Percent_Identity=45.959595959596, Blast_Score=317, Evalue=1e-87,
Organism=Escherichia coli, GI1790437, Length=312, Percent_Identity=47.4358974358974, Blast_Score=256, Evalue=4e-69,
Organism=Escherichia coli, GI1788550, Length=353, Percent_Identity=39.3767705382436, Blast_Score=249, Evalue=4e-67,
Organism=Escherichia coli, GI1790299, Length=331, Percent_Identity=45.0151057401813, Blast_Score=245, Evalue=4e-66,
Organism=Escherichia coli, GI1789233, Length=321, Percent_Identity=40.1869158878505, Blast_Score=231, Evalue=1e-61,
Organism=Escherichia coli, GI1788905, Length=238, Percent_Identity=48.7394957983193, Blast_Score=223, Evalue=3e-59,
Organism=Escherichia coli, GI87082152, Length=294, Percent_Identity=44.2176870748299, Blast_Score=221, Evalue=1e-58,
Organism=Escherichia coli, GI87081872, Length=321, Percent_Identity=40.4984423676012, Blast_Score=216, Evalue=3e-57,
Organism=Escherichia coli, GI1786524, Length=341, Percent_Identity=43.9882697947214, Blast_Score=213, Evalue=3e-56,
Organism=Escherichia coli, GI1787583, Length=314, Percent_Identity=40.1273885350318, Blast_Score=208, Evalue=8e-55,
Organism=Escherichia coli, GI1789828, Length=239, Percent_Identity=35.5648535564854, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI87081858, Length=305, Percent_Identity=30.8196721311475, Blast_Score=123, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 57428; Mature: 57428

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGTGLPVVGQDDVHRRIVEEAIELVLSTVDLQAVLERTGRLLRRHFGETRVAINRLSAE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCC
DPTRAEVVLVSDPRQPSPEVGTSFPLAGSAAGEALAKRLPCVVDPLRPREPRWREEPLLA
CCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEE
AYGYGSLVSFPLVFENELLGTLDIAHPPAEGLLDCCFEVARQVAHLVAIALHNSLMVEEV
EECCHHHHHCCHHCCCCCHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRLNRLLGRENALLKEEIRQIKRDSRYVAESEGMRAVVERVRLVAPSTTTVLVRGETGTG
HHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHCCCCCEEEEEECCCCCC
KEGLARMVHEFSPRFNAPFVAANLGAIPEGLIESELFGHEKGAFTGATRRRAGRFEQADG
HHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCC
GTLFLDEVGDAPPSVQVRLLRVLQERVVERVGGTEPVKVDVRVIAATNRNLEEMVARGTF
CEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCHHHHHHCCCH
RADLYYRLAVFPIELPPLRERREDVRPLGTYFLAKQAALMHRRPPRVGEDVWRALEAYGW
HHHHEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCC
PGNVRELENFLQRALILSPGQELSLPALPTAATQASPAAAAAAEPAPPGRFDDEVRELIE
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHH
RALEHAGGRVYGPGGAAALLGLRPTTLQGKMKKYGVGNPGRPTGT
HHHHHCCCEEECCCCHHHEECCCCCCCCCHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MVGTGLPVVGQDDVHRRIVEEAIELVLSTVDLQAVLERTGRLLRRHFGETRVAINRLSAE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCC
DPTRAEVVLVSDPRQPSPEVGTSFPLAGSAAGEALAKRLPCVVDPLRPREPRWREEPLLA
CCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEE
AYGYGSLVSFPLVFENELLGTLDIAHPPAEGLLDCCFEVARQVAHLVAIALHNSLMVEEV
EECCHHHHHCCHHCCCCCHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRLNRLLGRENALLKEEIRQIKRDSRYVAESEGMRAVVERVRLVAPSTTTVLVRGETGTG
HHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHCCCCCEEEEEECCCCCC
KEGLARMVHEFSPRFNAPFVAANLGAIPEGLIESELFGHEKGAFTGATRRRAGRFEQADG
HHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCC
GTLFLDEVGDAPPSVQVRLLRVLQERVVERVGGTEPVKVDVRVIAATNRNLEEMVARGTF
CEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCHHHHHHCCCH
RADLYYRLAVFPIELPPLRERREDVRPLGTYFLAKQAALMHRRPPRVGEDVWRALEAYGW
HHHHEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCC
PGNVRELENFLQRALILSPGQELSLPALPTAATQASPAAAAAAEPAPPGRFDDEVRELIE
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHH
RALEHAGGRVYGPGGAAALLGLRPTTLQGKMKKYGVGNPGRPTGT
HHHHHCCCEEECCCCHHHEECCCCCCCCCHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]