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The map label for this gene is tgt [H]

Identifier: 220917619

GI number: 220917619

Start: 2785250

End: 2787289

Strand: Direct

Name: tgt [H]

Synonym: A2cp1_2519

Alternate gene names: 220917619

Gene position: 2785250-2787289 (Clockwise)

Preceding gene: 220917618

Following gene: 220917620

Centisome position: 55.38

GC content: 77.35

Gene sequence:

>2040_bases
ATGCCCTTCCGCTTCCACCCCGGCCCGAGGGACGCCGAGACCCGCGCCCGGCGCGGCACGCTCGAGACGCCGCACGGGCG
CGTCGAGACCCCCGCCTTCATGCCGGTGGGCACGCGCGCCAGCGTCACCGGGCTCACCCCCGCCGACGTGCGCGAGGCGG
GCGCCGAGGTCATCCTCGGCAACACCTACCACCTGCTGCTCCGCCCCGGGCCCGAGGCGTTCCGCGCGCTCGGCGGCATC
CACCGCTTCATGCGCTGGGACGGCCCGATCCTCACCGACTCCGGCGGCTTCCAGATCTTCTCGCTCTCGCACGACCGCGT
CATCACCGAGGCGGGCGCGCGCTTCCGCAGCTACGTGGACGGACGCCCGCAGCTCCTCACCCCCGAGTCCTCCATCGCGA
TGCAGACCGCGATCGGCTCGGACGTGATGATGGTGCTCGACGTCTGCCTGCCCTCCACCGCCGAGGCGGCCGAGATCCGC
GCCGCCATGGAGCGCACCCACCGCTGGGCGCTGCGCAGCCTGGCCGCGCGCACCAACCCCGAGCAGGCGCTCTTCGCCAT
CGTCCAGGGCGGCCTCGACCCGGCGCTGCGCGCCGCCTCGGCCGGCTTCCTCACCCAGCACCCGTTCGACGGCTTCGCCA
TCGGCGGGCTCGCGGTCGGCGACACCCGGGCCGAGCGCGGCGAGGTGGTGGCGCGCGCCGCCGAGCTGCTCCCGCTCGAT
CGCCCGCGCTACCTCATGGGCGTGGGCACGCCGCCCGACCTCCTCGAGGCCATCGGCCACGGCGTGGACATGTTCGACTG
CATCCTGCCCACCACGCTCGCCTGGCAGGGCACCGCCTTCACCTCCACCGGCCGCGTGCGCCTCACCCGCACCGAGCACC
GGCTCTCCGAGCTCCCGCTCGACGCCGCCTGCGGCTGCGACACCTGCCGCGGCTGGAGCCGCGCCTACCTGCACCACCTG
TTCAAGTGCCGCGAGCCCCTCGGGCCGCGCCTCGTGTCCGTCCACAACCTCCGCCACTACCTCGACCTCATGCGCGGCGC
GCGCGCGGCCATCGAGGCCGGCCGCTACGGCGCCTACCAGCGCGAGCGGCTCGAGGCGCTCGACCGCCACGAGCACGACC
GCAGCGCACGCCCACCGGGCCGCCGCCGCCTCCCCGAGCCGCCGCCGCCGACGCCCGCGGCCCCGCCGGCGGAGGCGCCG
CGCGACGGCGCCCGCTTCCACCTCGTCGTCACGTCCCAGGGCGCGCCCGCGGTGCGCGACTCGGAGTCCGGCGAGGTGAT
GCACCCGGTGATCGGCCCGGCGGTGGAGGCGGAGCGGCTGTACGTGGCCCAGTCGCGCCTCCGCGAGCGGCTCGCCGAGC
CGGGGCCGCCGCTGGTGCTGCTCGACGTGGGGCTGGGCGCCGGCTCGAACGCGCTCGCCGCGATCCGCGCGGCCGGGGCG
GTCCCGCCGGGCGGACGCGGCCTGGAGATCGTCAGCTTCGAGCGCGATCTCGGGGCGCTCGGGCTGGCGACGTCGGACGA
GGGCGCGGCGCGGCTGGGCCTCGGGCCGGAGGACCTCGACGCGGCGCGGGCGCTCCTGCGCGACGGCGCCTGGGGCTCGG
CGCGCGTGCGCTGGCGGATCGTCCTGGGCGACCTGCTGGAGACGCTGCCGCGGCAGGACCTCCGCGCCGAGGTGATCTTC
TGGGATCCGTTCAGCCCGCGCGCGAACCCGGGCCTGTGGACGCTGGGGGCGTTCGCCGCCGCATGGGCGCGCTCCGGCCC
GCGCTGCGCGCTGTACACGTACAGCACGGCCACCGCGACGCGCTCGGCCCTGCTCCTCGCCGGCTTCCACGTGGGCGTGG
GCGACCCGAGCGGCCCGAAGGAGCAGACCACCGCGGCCGCGACGGATCCGGCGCTCCTGGCGCGCCCGCTCGACGCGCGC
TGGCTGGCGCGGCTCGAGCGCTCCTCGGCGCCGTTCCCGCCGGACGCGCCTCCCGACGCGCTCGCGCGGGTGCGCGCGCA
CCCGCAGTTCTCGGCGCGGCGCGAGGAGGAGGTCGCGTGA

Upstream 100 bases:

>100_bases
GCCGTGGCGGTGGAGCGGGTCCTCAGGCTGCGCGGCACCTGGGAGGCCGGCTGCGCCGAGGCCGCGCACGGCCACCTCCC
CCGCGCATAGGATCTGCCCG

Downstream 100 bases:

>100_bases
CCGCCTACCGCCCGAGCCGCTGGCTCCCGGGCGCGCATGCCATAACCGTGTTCGCGAGCGTGGCGCGCCCGCTGCCGCGC
CCGCCCGCCGTCCGCGAGCG

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 679; Mature: 678

Protein sequence:

>679_residues
MPFRFHPGPRDAETRARRGTLETPHGRVETPAFMPVGTRASVTGLTPADVREAGAEVILGNTYHLLLRPGPEAFRALGGI
HRFMRWDGPILTDSGGFQIFSLSHDRVITEAGARFRSYVDGRPQLLTPESSIAMQTAIGSDVMMVLDVCLPSTAEAAEIR
AAMERTHRWALRSLAARTNPEQALFAIVQGGLDPALRAASAGFLTQHPFDGFAIGGLAVGDTRAERGEVVARAAELLPLD
RPRYLMGVGTPPDLLEAIGHGVDMFDCILPTTLAWQGTAFTSTGRVRLTRTEHRLSELPLDAACGCDTCRGWSRAYLHHL
FKCREPLGPRLVSVHNLRHYLDLMRGARAAIEAGRYGAYQRERLEALDRHEHDRSARPPGRRRLPEPPPPTPAAPPAEAP
RDGARFHLVVTSQGAPAVRDSESGEVMHPVIGPAVEAERLYVAQSRLRERLAEPGPPLVLLDVGLGAGSNALAAIRAAGA
VPPGGRGLEIVSFERDLGALGLATSDEGAARLGLGPEDLDAARALLRDGAWGSARVRWRIVLGDLLETLPRQDLRAEVIF
WDPFSPRANPGLWTLGAFAAAWARSGPRCALYTYSTATATRSALLLAGFHVGVGDPSGPKEQTTAAATDPALLARPLDAR
WLARLERSSAPFPPDAPPDALARVRAHPQFSARREEEVA

Sequences:

>Translated_679_residues
MPFRFHPGPRDAETRARRGTLETPHGRVETPAFMPVGTRASVTGLTPADVREAGAEVILGNTYHLLLRPGPEAFRALGGI
HRFMRWDGPILTDSGGFQIFSLSHDRVITEAGARFRSYVDGRPQLLTPESSIAMQTAIGSDVMMVLDVCLPSTAEAAEIR
AAMERTHRWALRSLAARTNPEQALFAIVQGGLDPALRAASAGFLTQHPFDGFAIGGLAVGDTRAERGEVVARAAELLPLD
RPRYLMGVGTPPDLLEAIGHGVDMFDCILPTTLAWQGTAFTSTGRVRLTRTEHRLSELPLDAACGCDTCRGWSRAYLHHL
FKCREPLGPRLVSVHNLRHYLDLMRGARAAIEAGRYGAYQRERLEALDRHEHDRSARPPGRRRLPEPPPPTPAAPPAEAP
RDGARFHLVVTSQGAPAVRDSESGEVMHPVIGPAVEAERLYVAQSRLRERLAEPGPPLVLLDVGLGAGSNALAAIRAAGA
VPPGGRGLEIVSFERDLGALGLATSDEGAARLGLGPEDLDAARALLRDGAWGSARVRWRIVLGDLLETLPRQDLRAEVIF
WDPFSPRANPGLWTLGAFAAAWARSGPRCALYTYSTATATRSALLLAGFHVGVGDPSGPKEQTTAAATDPALLARPLDAR
WLARLERSSAPFPPDAPPDALARVRAHPQFSARREEEVA
>Mature_678_residues
PFRFHPGPRDAETRARRGTLETPHGRVETPAFMPVGTRASVTGLTPADVREAGAEVILGNTYHLLLRPGPEAFRALGGIH
RFMRWDGPILTDSGGFQIFSLSHDRVITEAGARFRSYVDGRPQLLTPESSIAMQTAIGSDVMMVLDVCLPSTAEAAEIRA
AMERTHRWALRSLAARTNPEQALFAIVQGGLDPALRAASAGFLTQHPFDGFAIGGLAVGDTRAERGEVVARAAELLPLDR
PRYLMGVGTPPDLLEAIGHGVDMFDCILPTTLAWQGTAFTSTGRVRLTRTEHRLSELPLDAACGCDTCRGWSRAYLHHLF
KCREPLGPRLVSVHNLRHYLDLMRGARAAIEAGRYGAYQRERLEALDRHEHDRSARPPGRRRLPEPPPPTPAAPPAEAPR
DGARFHLVVTSQGAPAVRDSESGEVMHPVIGPAVEAERLYVAQSRLRERLAEPGPPLVLLDVGLGAGSNALAAIRAAGAV
PPGGRGLEIVSFERDLGALGLATSDEGAARLGLGPEDLDAARALLRDGAWGSARVRWRIVLGDLLETLPRQDLRAEVIFW
DPFSPRANPGLWTLGAFAAAWARSGPRCALYTYSTATATRSALLLAGFHVGVGDPSGPKEQTTAAATDPALLARPLDARW
LARLERSSAPFPPDAPPDALARVRAHPQFSARREEEVA

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=357, Percent_Identity=43.6974789915966, Blast_Score=298, Evalue=1e-80,
Organism=Escherichia coli, GI1786607, Length=358, Percent_Identity=47.4860335195531, Blast_Score=342, Evalue=6e-95,
Organism=Caenorhabditis elegans, GI17544680, Length=350, Percent_Identity=42.5714285714286, Blast_Score=283, Evalue=2e-76,
Organism=Drosophila melanogaster, GI19920510, Length=347, Percent_Identity=42.9394812680115, Blast_Score=297, Evalue=1e-80,
Organism=Drosophila melanogaster, GI21355311, Length=211, Percent_Identity=28.436018957346, Blast_Score=90, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 73218; Mature: 73087

Theoretical pI: Translated: 7.92; Mature: 7.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFRFHPGPRDAETRARRGTLETPHGRVETPAFMPVGTRASVTGLTPADVREAGAEVILG
CCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEC
NTYHLLLRPGPEAFRALGGIHRFMRWDGPILTDSGGFQIFSLSHDRVITEAGARFRSYVD
CEEEEEECCCHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEECCCHHHHHHHHHHHHHCC
GRPQLLTPESSIAMQTAIGSDVMMVLDVCLPSTAEAAEIRAAMERTHRWALRSLAARTNP
CCCCEECCCHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCH
EQALFAIVQGGLDPALRAASAGFLTQHPFDGFAIGGLAVGDTRAERGEVVARAAELLPLD
HHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCEEECCEEECCCCHHHHHHHHHHHHHCCCC
RPRYLMGVGTPPDLLEAIGHGVDMFDCILPTTLAWQGTAFTSTGRVRLTRTEHRLSELPL
CCCEEEECCCCHHHHHHHHCCCHHHHHHHCHHHCCCCCEEECCCCEEEEEHHHHHHHCCC
DAACGCDTCRGWSRAYLHHLFKCREPLGPRLVSVHNLRHYLDLMRGARAAIEAGRYGAYQ
CHHCCCHHHCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
RERLEALDRHEHDRSARPPGRRRLPEPPPPTPAAPPAEAPRDGARFHLVVTSQGAPAVRD
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCC
SESGEVMHPVIGPAVEAERLYVAQSRLRERLAEPGPPLVLLDVGLGAGSNALAAIRAAGA
CCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHCC
VPPGGRGLEIVSFERDLGALGLATSDEGAARLGLGPEDLDAARALLRDGAWGSARVRWRI
CCCCCCCEEEEEECCCCCCEEECCCCCCCEECCCCHHHHHHHHHHHHCCCCCCCEEEEEE
VLGDLLETLPRQDLRAEVIFWDPFSPRANPGLWTLGAFAAAWARSGPRCALYTYSTATAT
HHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEEHHHHHHHHHHCCCCCEEEEEECCHHHH
RSALLLAGFHVGVGDPSGPKEQTTAAATDPALLARPLDARWLARLERSSAPFPPDAPPDA
HHHHEEEEEEECCCCCCCCCHHCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCCCCHHH
LARVRAHPQFSARREEEVA
HHHHHCCCCHHCCHHHCCC
>Mature Secondary Structure 
PFRFHPGPRDAETRARRGTLETPHGRVETPAFMPVGTRASVTGLTPADVREAGAEVILG
CCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEC
NTYHLLLRPGPEAFRALGGIHRFMRWDGPILTDSGGFQIFSLSHDRVITEAGARFRSYVD
CEEEEEECCCHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEECCCHHHHHHHHHHHHHCC
GRPQLLTPESSIAMQTAIGSDVMMVLDVCLPSTAEAAEIRAAMERTHRWALRSLAARTNP
CCCCEECCCHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCH
EQALFAIVQGGLDPALRAASAGFLTQHPFDGFAIGGLAVGDTRAERGEVVARAAELLPLD
HHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCEEECCEEECCCCHHHHHHHHHHHHHCCCC
RPRYLMGVGTPPDLLEAIGHGVDMFDCILPTTLAWQGTAFTSTGRVRLTRTEHRLSELPL
CCCEEEECCCCHHHHHHHHCCCHHHHHHHCHHHCCCCCEEECCCCEEEEEHHHHHHHCCC
DAACGCDTCRGWSRAYLHHLFKCREPLGPRLVSVHNLRHYLDLMRGARAAIEAGRYGAYQ
CHHCCCHHHCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
RERLEALDRHEHDRSARPPGRRRLPEPPPPTPAAPPAEAPRDGARFHLVVTSQGAPAVRD
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCC
SESGEVMHPVIGPAVEAERLYVAQSRLRERLAEPGPPLVLLDVGLGAGSNALAAIRAAGA
CCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHCC
VPPGGRGLEIVSFERDLGALGLATSDEGAARLGLGPEDLDAARALLRDGAWGSARVRWRI
CCCCCCCEEEEEECCCCCCEEECCCCCCCEECCCCHHHHHHHHHHHHCCCCCCCEEEEEE
VLGDLLETLPRQDLRAEVIFWDPFSPRANPGLWTLGAFAAAWARSGPRCALYTYSTATAT
HHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEEHHHHHHHHHHCCCCCEEEEEECCHHHH
RSALLLAGFHVGVGDPSGPKEQTTAAATDPALLARPLDARWLARLERSSAPFPPDAPPDA
HHHHEEEEEEECCCCCCCCCHHCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCCCCHHH
LARVRAHPQFSARREEEVA
HHHHHCCCCHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]