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The map label for this gene is nrgA [H]

Identifier: 220917564

GI number: 220917564

Start: 2722786

End: 2724156

Strand: Direct

Name: nrgA [H]

Synonym: A2cp1_2464

Alternate gene names: 220917564

Gene position: 2722786-2724156 (Clockwise)

Preceding gene: 220917563

Following gene: 220917565

Centisome position: 54.14

GC content: 75.78

Gene sequence:

>1371_bases
GTGAAGAAGCTCCTCGCACCGCTGCTGCTGGCCCTGCCCGCGCTCGCGTCCGCCGCCGACGGCATCGACACCGGCGACAC
CGCGCTCGTGCTCGTGAGCGCCGGGCTGGTCATGCTCATGACCCCGGGCCTCGCGTTCTTCTACGGCGGCCTGGTCCGCG
CCAAGAACGTCGTCCACACCATGAACCTGTCGATCGTGTGCATGGCGATCGTGGGCGTGCTGTGGGCGACGATCGGCTAC
TCGCTCGCGTTCGCCCCCGGGCCGGGCGCGCTCGACCGCGTGGTGGGCGGCCTGCGCTGGGCCGGGCTCGCCGGCGTGGG
CGACGCGCCCGAGCCGTCCCTCGCGGCCACGGTGCCGCACTCCGCGTTCATGCTCTTCCAGGCGATGTTCGCCGTGATCA
CGCCGGCGCTCATCTCGGGCGCGGTGGTCGAGCGCATGCGCATCAAGGCCTACGTGCTGTTCGTCGCGCTGTGGAGCCTG
GTGGTGTACACGCCGGTGGCGCACTGGGTGTGGGCGCCGGGCGGCTGGCTCCGCAACCTCGGCGTCCTCGACTTCGCCGG
CGGCACGGTGGTGCACGTGAACGCCGCCGCGGCGGCGCTGGTGGGCGCGGTGGTGCTGGGCAAGCGCCGCGGCCTGCGCG
CCCCCACGGTGCTCCCGCACAGCGTCCCGTTCGCCATCCTCGGCGCGGGCCTGCTCTGGTTCGGCTGGCTCGGCTTCAAC
GGCGGCAGCGCGCTGGCCGCGAACGGCCTCGCCAGCACCGCCTTCGCGAACACCTTCTTCGCGCCCGCGGCGGCGGCCGC
CGCCTGGGGGCTCGCCGAGCTGCTGATGCACGGGAAGGTCTCGGGCGTCGGGCTCGCCTCCGGCGCGGTGGCCGGCATGG
TGGCCATCACGCCGGCCGCCGGCTTCATCCGGCCCGGCGCGAGCCTGGTGCTCGGTGCGGTCGCGGCGCTCGCCTCCTTC
GGCGCCATCCGGCTGCGCCCGCGGCTCGGGCTGGACGACGCGCTGGACGTCTTCGCGGTCCACGGCGTGGCGGCCACGCT
CGGCGCGGTGCTCACCGGCGCGCTCGCCACCACCTCGGTGAACGCCGCCGGCGCGGACGCGAGCCTGGCGCTGGTGGGCA
AGCAGGCCCTGGGCGTGGCCGTGACGCTGGTGTTCTCCGGCGGGCTGTCCTACGGGCTGTACCGGCTGGTGGCGCTCGTC
ACGCCGTTGCGCGCCGACGAGCAGGACGAGTGGACCGGCCTCGACCAGGCCGAGGCCGGCGAGCGGGCGTACCTCTCCAC
CGACCTCGAGACGGCCGGCGCCGCGCCCAGCGCCGCGACGCACGCGCCGGTGCCGGTGGCCCGCCCGAGCCCCGGCTCCA
GCGCCGCCTGA

Upstream 100 bases:

>100_bases
GAGCGGGCTCCGGAACGCCCGCGATCCGCGCACCTCCGGCGGGCACGGGCGCTGCAGTGGCCCGGGGAGCGCGCCCCGCG
CACCCTGCCCTGGAGGCTCC

Downstream 100 bases:

>100_bases
TCGCGAGCCGCCCCGCGCCGCCCCGCGCCCGCCCCCGGCTCCCGGGGGCGGGCGTTCACGTTTCGTCACGACGCGCTCCG
TGAACGTGCGCCGCTTGCTC

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Membrane protein NrgA; Protein AmtB [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MKKLLAPLLLALPALASAADGIDTGDTALVLVSAGLVMLMTPGLAFFYGGLVRAKNVVHTMNLSIVCMAIVGVLWATIGY
SLAFAPGPGALDRVVGGLRWAGLAGVGDAPEPSLAATVPHSAFMLFQAMFAVITPALISGAVVERMRIKAYVLFVALWSL
VVYTPVAHWVWAPGGWLRNLGVLDFAGGTVVHVNAAAAALVGAVVLGKRRGLRAPTVLPHSVPFAILGAGLLWFGWLGFN
GGSALAANGLASTAFANTFFAPAAAAAAWGLAELLMHGKVSGVGLASGAVAGMVAITPAAGFIRPGASLVLGAVAALASF
GAIRLRPRLGLDDALDVFAVHGVAATLGAVLTGALATTSVNAAGADASLALVGKQALGVAVTLVFSGGLSYGLYRLVALV
TPLRADEQDEWTGLDQAEAGERAYLSTDLETAGAAPSAATHAPVPVARPSPGSSAA

Sequences:

>Translated_456_residues
MKKLLAPLLLALPALASAADGIDTGDTALVLVSAGLVMLMTPGLAFFYGGLVRAKNVVHTMNLSIVCMAIVGVLWATIGY
SLAFAPGPGALDRVVGGLRWAGLAGVGDAPEPSLAATVPHSAFMLFQAMFAVITPALISGAVVERMRIKAYVLFVALWSL
VVYTPVAHWVWAPGGWLRNLGVLDFAGGTVVHVNAAAAALVGAVVLGKRRGLRAPTVLPHSVPFAILGAGLLWFGWLGFN
GGSALAANGLASTAFANTFFAPAAAAAAWGLAELLMHGKVSGVGLASGAVAGMVAITPAAGFIRPGASLVLGAVAALASF
GAIRLRPRLGLDDALDVFAVHGVAATLGAVLTGALATTSVNAAGADASLALVGKQALGVAVTLVFSGGLSYGLYRLVALV
TPLRADEQDEWTGLDQAEAGERAYLSTDLETAGAAPSAATHAPVPVARPSPGSSAA
>Mature_456_residues
MKKLLAPLLLALPALASAADGIDTGDTALVLVSAGLVMLMTPGLAFFYGGLVRAKNVVHTMNLSIVCMAIVGVLWATIGY
SLAFAPGPGALDRVVGGLRWAGLAGVGDAPEPSLAATVPHSAFMLFQAMFAVITPALISGAVVERMRIKAYVLFVALWSL
VVYTPVAHWVWAPGGWLRNLGVLDFAGGTVVHVNAAAAALVGAVVLGKRRGLRAPTVLPHSVPFAILGAGLLWFGWLGFN
GGSALAANGLASTAFANTFFAPAAAAAAWGLAELLMHGKVSGVGLASGAVAGMVAITPAAGFIRPGASLVLGAVAALASF
GAIRLRPRLGLDDALDVFAVHGVAATLGAVLTGALATTSVNAAGADASLALVGKQALGVAVTLVFSGGLSYGLYRLVALV
TPLRADEQDEWTGLDQAEAGERAYLSTDLETAGAAPSAATHAPVPVARPSPGSSAA

Specific function: Functions as an ammonium and methylammonium transporter in the absence of glutamine. Required for ammonium utilization at low concentrations or at low pH values, when ammonium is the single nitrogen source. Required for binding of NrgB to the membrane [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=431, Percent_Identity=41.7633410672854, Blast_Score=291, Evalue=9e-80,
Organism=Caenorhabditis elegans, GI17550182, Length=449, Percent_Identity=31.1804008908686, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17550180, Length=425, Percent_Identity=29.4117647058824, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI32564042, Length=417, Percent_Identity=26.378896882494, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI133931100, Length=334, Percent_Identity=28.1437125748503, Blast_Score=109, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6324187, Length=423, Percent_Identity=35.4609929078014, Blast_Score=217, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6325396, Length=467, Percent_Identity=32.5481798715203, Blast_Score=215, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6321559, Length=411, Percent_Identity=33.3333333333333, Blast_Score=207, Evalue=3e-54,
Organism=Drosophila melanogaster, GI161078314, Length=416, Percent_Identity=28.8461538461538, Blast_Score=128, Evalue=6e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 45744; Mature: 45744

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: PS00027 HOMEOBOX_1 ; PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLLAPLLLALPALASAADGIDTGDTALVLVSAGLVMLMTPGLAFFYGGLVRAKNVVHT
CHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCHHHHHCCCHHHHHHHHHHHHHHHHH
MNLSIVCMAIVGVLWATIGYSLAFAPGPGALDRVVGGLRWAGLAGVGDAPEPSLAATVPH
HHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEECCH
SAFMLFQAMFAVITPALISGAVVERMRIKAYVLFVALWSLVVYTPVAHWVWAPGGWLRNL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHC
GVLDFAGGTVVHVNAAAAALVGAVVLGKRRGLRAPTVLPHSVPFAILGAGLLWFGWLGFN
CCEEECCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
GGSALAANGLASTAFANTFFAPAAAAAAWGLAELLMHGKVSGVGLASGAVAGMVAITPAA
CCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHEECCCC
GFIRPGASLVLGAVAALASFGAIRLRPRLGLDDALDVFAVHGVAATLGAVLTGALATTSV
CCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NAAGADASLALVGKQALGVAVTLVFSGGLSYGLYRLVALVTPLRADEQDEWTGLDQAEAG
CCCCCCCEEEEHHHHHHHHHEEEHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
ERAYLSTDLETAGAAPSAATHAPVPVARPSPGSSAA
CCEEEECCCHHCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKKLLAPLLLALPALASAADGIDTGDTALVLVSAGLVMLMTPGLAFFYGGLVRAKNVVHT
CHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCHHHHHCCCHHHHHHHHHHHHHHHHH
MNLSIVCMAIVGVLWATIGYSLAFAPGPGALDRVVGGLRWAGLAGVGDAPEPSLAATVPH
HHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEECCH
SAFMLFQAMFAVITPALISGAVVERMRIKAYVLFVALWSLVVYTPVAHWVWAPGGWLRNL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHC
GVLDFAGGTVVHVNAAAAALVGAVVLGKRRGLRAPTVLPHSVPFAILGAGLLWFGWLGFN
CCEEECCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
GGSALAANGLASTAFANTFFAPAAAAAAWGLAELLMHGKVSGVGLASGAVAGMVAITPAA
CCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHEECCCC
GFIRPGASLVLGAVAALASFGAIRLRPRLGLDDALDVFAVHGVAATLGAVLTGALATTSV
CCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NAAGADASLALVGKQALGVAVTLVFSGGLSYGLYRLVALVTPLRADEQDEWTGLDQAEAG
CCCCCCCEEEEHHHHHHHHHEEEHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
ERAYLSTDLETAGAAPSAATHAPVPVARPSPGSSAA
CCEEEECCCHHCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8282685; 9353933; 9384377 [H]