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The map label for this gene is yafJ [C]

Identifier: 220917550

GI number: 220917550

Start: 2708651

End: 2709424

Strand: Direct

Name: yafJ [C]

Synonym: A2cp1_2450

Alternate gene names: 220917550

Gene position: 2708651-2709424 (Clockwise)

Preceding gene: 220917549

Following gene: 220917551

Centisome position: 53.86

GC content: 75.84

Gene sequence:

>774_bases
ATGTGCCGCCTCTTCGGTCAGCACGCCCACCCCGGCCGCGATGCCTGCGAGCCGCTCTGCAGCGCAGAGAACGCCCTCCG
GTTCCAGTCGCACCGGCACCCGCACGGCTGGGGGATCGCCTGGTACGTGCAGGGGTCGCCGCTCGTGCGCCGGGGCATCC
TCCCCGCCCACGCCGACGCCGCCTTCGTCGAGGCGGGGCGCGAGATCCGCTCGGCGGTGGTGGTCGCGCACGTGCGCGAG
GCCAGCGTCGGGCCGGTGCTCCGCGAGAACACCCACCCGTTCGTGCACGATCGCTGGGTGTTCGCGCACAACGGGACGGT
CGCCCGCTTCAAGGACGCCCCGGACGTGCGCGAGCGGATCCTCGCCGAGATCGACCCCGACCTGCGCGGCCGCATCCGCG
GCGACACCGACAGCGAGCGCTGCTTCTACCTGTTCCTGACCCGGCTGCGCGCGCGCGGCGGGCTCGAGGCGCCCGGCGTC
GAGGCGGTGCGCGCGGCGCTCGCGGCCACCACCGACGCCGTGCTGCGCATCGCGGACGCGGTGCCGTCGCCCAAGCCGAG
CTCGCTCACCTTCCTCGTGTCGGACGGCCGGCTCCTCGCCGCCTGCCGCCGCGGCCGCACGCTGCACGCCGCGAGCGACG
CCGGGCCGCGCCACGCGTTCGTGGTGGCGAGCGAGCGGATCGGCCGAGCCCCTTGGAGCGAGGTGCCCGAGGGAGGCTTC
GTCGCCACCGAGGACGGGATCCGGGTGGTTGACGCGCCGCTGCACGCGCCCTGA

Upstream 100 bases:

>100_bases
CGCTCTGACGAGCACGCGAGGCGATCCCGCCCCGCGGCGGGCGCGCCCCCGCCCGGATTGGATCGGCGGGCGGCGCCCTG
CTAGATTCCCTGCGTCCGCG

Downstream 100 bases:

>100_bases
GCCCCTCCGCGCGGGGACCGCCCCGGCGCTTCCCCCTGCCCCGTGCATCGCAGGCGCGGCGGCTCGACCCGCCCGCGCGA
CCCCCGCCGTGAGGCCGCCG

Product: glutamine amidotransferase class-II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MCRLFGQHAHPGRDACEPLCSAENALRFQSHRHPHGWGIAWYVQGSPLVRRGILPAHADAAFVEAGREIRSAVVVAHVRE
ASVGPVLRENTHPFVHDRWVFAHNGTVARFKDAPDVRERILAEIDPDLRGRIRGDTDSERCFYLFLTRLRARGGLEAPGV
EAVRAALAATTDAVLRIADAVPSPKPSSLTFLVSDGRLLAACRRGRTLHAASDAGPRHAFVVASERIGRAPWSEVPEGGF
VATEDGIRVVDAPLHAP

Sequences:

>Translated_257_residues
MCRLFGQHAHPGRDACEPLCSAENALRFQSHRHPHGWGIAWYVQGSPLVRRGILPAHADAAFVEAGREIRSAVVVAHVRE
ASVGPVLRENTHPFVHDRWVFAHNGTVARFKDAPDVRERILAEIDPDLRGRIRGDTDSERCFYLFLTRLRARGGLEAPGV
EAVRAALAATTDAVLRIADAVPSPKPSSLTFLVSDGRLLAACRRGRTLHAASDAGPRHAFVVASERIGRAPWSEVPEGGF
VATEDGIRVVDAPLHAP
>Mature_257_residues
MCRLFGQHAHPGRDACEPLCSAENALRFQSHRHPHGWGIAWYVQGSPLVRRGILPAHADAAFVEAGREIRSAVVVAHVRE
ASVGPVLRENTHPFVHDRWVFAHNGTVARFKDAPDVRERILAEIDPDLRGRIRGDTDSERCFYLFLTRLRARGGLEAPGV
EAVRAALAATTDAVLRIADAVPSPKPSSLTFLVSDGRLLAACRRGRTLHAASDAGPRHAFVVASERIGRAPWSEVPEGGF
VATEDGIRVVDAPLHAP

Specific function: Unknown

COG id: COG0121

COG function: function code R; Predicted glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786417, Length=208, Percent_Identity=29.3269230769231, Blast_Score=67, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324138, Length=194, Percent_Identity=29.3814432989691, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00310 GATase_2 [H]

EC number: NA

Molecular weight: Translated: 27976; Mature: 27976

Theoretical pI: Translated: 8.53; Mature: 8.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCRLFGQHAHPGRDACEPLCSAENALRFQSHRHPHGWGIAWYVQGSPLVRRGILPAHADA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHCCCCCCCCH
AFVEAGREIRSAVVVAHVREASVGPVLRENTHPFVHDRWVFAHNGTVARFKDAPDVRERI
HHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCEECEEEEEECCEEEEECCCHHHHHHH
LAEIDPDLRGRIRGDTDSERCFYLFLTRLRARGGLEAPGVEAVRAALAATTDAVLRIADA
HHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VPSPKPSSLTFLVSDGRLLAACRRGRTLHAASDAGPRHAFVVASERIGRAPWSEVPEGGF
CCCCCCCCEEEEEECCEEEEHHHCCCEEEECCCCCCCEEEEEEHHHCCCCCHHHCCCCCE
VATEDGIRVVDAPLHAP
EEECCCCEEEECCCCCC
>Mature Secondary Structure
MCRLFGQHAHPGRDACEPLCSAENALRFQSHRHPHGWGIAWYVQGSPLVRRGILPAHADA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHCCCCCCCCH
AFVEAGREIRSAVVVAHVREASVGPVLRENTHPFVHDRWVFAHNGTVARFKDAPDVRERI
HHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCEECEEEEEECCEEEEECCCHHHHHHH
LAEIDPDLRGRIRGDTDSERCFYLFLTRLRARGGLEAPGVEAVRAALAATTDAVLRIADA
HHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VPSPKPSSLTFLVSDGRLLAACRRGRTLHAASDAGPRHAFVVASERIGRAPWSEVPEGGF
CCCCCCCCEEEEEECCEEEEHHHCCCEEEECCCCCCCEEEEEEHHHCCCCCHHHCCCCCE
VATEDGIRVVDAPLHAP
EEECCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]